Experiences of Shaukat Khanum Memorial Cancer Hospital and Research Centre Biobank during COVID-19 Pandemic
Kashif
Asghar
Shaukat Khanum Memorial Cancer Hospital and Research Centre, Lahore, Pakistan.
author
Asim
Farooq
Shaukat Khanum Memorial Cancer Hospital and Research Centre, Lahore, Pakistan.
author
Asif
Loya
Shaukat Khanum Memorial Cancer Hospital and Research Centre, Lahore, Pakistan.
author
text
article
2022
eng
Pakistan has an approximate population of 228.9 million. In 2020, 178,388 new cancer cases were diagnosed in Pakistan. In 2019, we established the biobanking facility at Shaukat Khanum Memorial Cancer Hospital and Research Centre, Lahore, Pakistan. Shaukat Khanum Memorial Cancer Hospital and Research Centre is a tertiary care charitable, not-for-profit cancer hospital in Pakistan. In 2020-21, 22,745 new cancer patients were registered in the Shaukat Khanum Memorial Cancer Hospital and Research Centre for cancer treatment. The hospital treats around 75% of accepted cancer patients free of charge, regardless of race or nationality. In December 2019, a novel coronavirus SARS-Cov-2 (COVID-19) was identified in China. The World Health Organization acknowledged the COVID-19 outbreak as a pandemic. Pakistan was hit by the first wave of COVID-19 in March 2020. We have highlighted the challenges faced during the COVID-19 pandemic. We emphasized the significance of collaborations between low and middle-income countries’ biobanks and international biobanks to achieve the global perspective of biobanking.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
2879
2880
https://journal.waocp.org/article_90279_c4fd67565ffc4a3eb9fc316ffabf959a.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.2879
Systematic Review and Meta-Synthesis about Patients with Hematological Malignancy and Palliative Care
Mohammad
Alnaeem
School of Nursing, Irbid National University, Amman, Jordan.
author
Hala
Bawadi
School of Nursing, The University of Jordan Amman, Jordan.
author
text
article
2022
eng
Objective: The current study aims to review, appraise, and synthesize the available studies and recommend the significant clinical implications for healthcare professionals to understand the existing findings of palliative care experience among patients with hematology malignancy. Methods: After excluding the registered or ongoing systematic reviews in the PROSPERO database regarding the lived experience of palliative care among patients with HM, our systematic review and meta-analysis protocol was registered in PROSPERO [CRD42021270311]. A search for published articles in English between January 2000 and December 2020 was conducted among different electronic databases using PRISMA guidelines 2020. Meta-synthesis was accomplished using the JBI meta-aggregation method to synthesize the findings. The implemented approach involved all qualitative research and mixed-method studies that included a qualitative part. Results: This review contained eight studies which led to 25 codes and seven categories. Finally, three synthesized themes were developed: (1) Approaching the end of life among patients with Hematology Malignancy, (2) submission and surrender of patients with Hematology Malignancy during their terminal stage, and (3) Entrance to the palliative care world. Therefore, realizing the importance of palliative care services to patients with Hematology Malignancy by providing evidence-based education and timely referral is crucial. Conclusion: There was a substantial increase in the HMs rate with late referral to palliative care services. The results of this review may draw attention to some issues reported by patients with Hematology Malignancy. Scaling up palliative care services for those patients is essential to minimize end-of-life suffering and the long-term impact of inadequate palliative care for patients with Hematology Malignancy.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
2881
2890
https://journal.waocp.org/article_90264_d27aa0d931dece26b8080369b2f3c484.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.2881
The Effects of Combined Vitamin E and C for Treatment of Non-Alcoholic Fatty Liver Disease (NAFLD): A Systematic Review and Meta-Analysis of Randomized Controlled Trials
Piyakorn
Poonyam
Division of Gastroenterology, Department of Internal Medicine, Faculty of Medicine, Srinakharinwirot University, Thailand.
author
Chawan
Kritsanaviparkporn
Division of Gastroenterology, Department of Internal Medicine, Faculty of Medicine, Srinakharinwirot University, Thailand.
author
Piyanant
Chommaitree
Department of Internal Medicine, HRH Princess Maha Chakri Sirindhorn Medical Center, Faculty of Medicine, Srinakharinwirot University, Nakhon Nayok, Thailand.
author
Asawin
Soodcharoen
Division of Gastroenterology, Department of Internal Medicine, Faculty of Medicine, Srinakharinwirot University, Thailand.
author
text
article
2022
eng
Objective: Antioxidant therapy is a promising treatment option for non-alcoholic fatty liver disease (NAFLD) after failure of lifestyle modification. We aimed to explore the efficacy of combined vitamin E and C therapy compared to no treatment for NAFLD. Methods: A literature search was performed in Ovid Embase, Ovid Medline, PubMed, Cochrane Library, Scopus, and Web of Science from inception to 28th April 2020. A systematic review and meta-analysis were conducted on randomized controlled trials that assessed vitamin E and C co-treatment in NAFLD. Quality of evidence was appraised using the Grading of Recommendations, Assessment, Development, and Evaluation (GRADE) approach. Assessed outcomes were changes in imaging findings, histological features, and serum transaminases. Subgroup analyses that compared adult versus children were further explored. Results: Four studies (n=260) satisfied our eligibility criteria. Vitamin co-treatment did not improve ultrasonographic liver brightness, histological parameters of hepatocyte injury (steatosis, lobular inflammation, and ballooning), fibrosis grading (standardized mean difference [SMD ]: 0.02, 95% CI: -0.40 to 0.45, I2=13%), serum aspartate transaminase (mean difference [MD]: -0.05, 95% CI: -2.59 to 2.50, I2=0%), and serum alanine transaminase (MD: 2.82, 95% CI: -2.11 to 7.76, I2=57%). Subgroup stratifications illustrated similar findings. Conclusion: Vitamin co-treatment may have limited efficacy in NAFLD. However, we have little confidence in our effect estimates due to bias and other major constraints.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
2891
2899
https://journal.waocp.org/article_90301_0fc0f5cf11d5800f3d32945e562526b4.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.2891
The Need for Nicotine De-addiction Services among Newly Diagnosed Tobacco-Related Head and Neck Cancer Patients, South India
Bharathnag
Nagappa
Department of Epidemiology, Institute of Liver and Biliary Sciences, New Delhi, India.
author
Mahalakshmy
Thulasingam
Department of Preventive and Social
Medicine, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India.
author
Jagadesan
Pandjatcharam
Department of Radiation
Oncology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India.
author
Sivaraman
Ganesan
Department of ENT,
Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India.
author
Manikandanesan
Sakthivel
ICMR, National Institute of
Epidemiology, Puducherry, India.
author
Sitanshu
Sekhar Kar
Department of Preventive and Social Medicine, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India.
author
text
article
2022
eng
Objectives: This study aimed to determine the proportion of tobacco-related head and neck cancer patients in need of nicotine de-addiction services at the time of diagnosis and factors associated with it. Methods: Facility-based cross-sectional study was conducted in a tertiary care center. Tobacco-related head and neck cancer patients with a past and present history of tobacco usage registered in cancer clinic from March 2016 to February 2017 were recruited. Participants were interviewed using a pretested and semi-structured questionnaire to gather information on the socio-demographic, clinical characteristics, and tobacco usage. Data were entered in EpiData v3.1 and analyzed using STATA v14. Results: Among 220 participants recruited in the study, 83% were males, 47% were >60 years of age, and 40% had no formal education. Around 49% were smoking tobacco during the treatment period, 41% used smokeless tobacco, and 10% used both smoking and smokeless. The majority (56%) of them had stage T4 tumors. Around 71% of participants required de-addiction services. Those of age more than 70 years (aRR (95%CI) 1.43 (1.1-1.9)), currently employed (aRR (95%CI) 1.5 (1.2-1.9)), living alone (aRR (95%CI) 1.6 (1.0-2.5)) or in a nuclear family (aRR (95%CI) 1.5 (1.2-2)), who initiated tobacco use at a younger age (aRR (95%CI) 1.5 (1.0-2.2) were in higher need of de-addiction services.Conclusion: The majority of tobacco-related head and neck cancer patients required nicotine de-addiction treatment. Hence de-addiction services should be established as an integral unit of cancer clinics.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
2901
2906
https://journal.waocp.org/article_90288_de9bad67313d5e2c3f085b1773785c54.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.2901
Epidemiology of Bladder Cancer in the Arab World: 2019 Global Burden of Disease Data
Ibrahim
Al Saidi
School of Medicine, University of Jordan, Amman, Jordan.
author
Ali
Mohamedabugroon
School of Medicine, University of Jordan, Amman, Jordan.
author
Amer
Sawalha
School of Medicine, University of Jordan, Amman, Jordan.
author
Iyad
Sultan
Department of Pediatric Hematology and Oncology, King Hussein
Cancer Center, Amman, Jordan.
author
text
article
2022
eng
Background: Urinary bladder cancer (UBC) has unique epidemiology. It is well known that some Arab countries have higher incidence of UBC due to certain risk factors, including schistosomiasis and smoking. Methods: We systematically reviewed the Global Burden of Disease study (GBD) database for the year 2019. We queried GBD study database using results tool for UBC in the 22 Arab countries and compared the age-standardized incidence rate (ASIR), death rate (ASDR), mortality-to-incidence ratio (MIR) and Disability-Adjusted Life Years (ASDALYs) in Arab countries with global values. Results: The Arab population represents 5.6% of the global population. There was an estimated number of 27,503 patients diagnosed with UBC in 2019 in the Arab world. The ASIR for developing UBC in the Arab countries was 9.9/100,000; which is higher than the average global ASIR (6.5/100,000). Specifically, the ASIR was higher than the global average in 11 out of the 22 Arab countries. Notably, the age-specific incidence in Egypt showed that younger individuals had higher incidence of UBC than other countries, reflecting unique epidemiology. It is estimated that 10,532 patients died of UBC in the Arab world in 2019. The average mortality-to-incidence ratio (MIR) was estimated to be 0.448. The burden of UBC as estimated by the number of DALYs in the Arab world was 272,976 years representing 4% of cancer burden in Arab countries and 6.2% of the global DALYs related to UBC. Conclusion: UBC has high incidence in many Arab countries, particularly in North Africa. Risk factors are known and are modifiable, making prevention the most optimal way to reduce disease burden. High MIR in many Arab countries reflects suboptimal management and a window for improvement.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
2907
2919
https://journal.waocp.org/article_90289_d37d9529cfaf6e952a00fee1fc45a990.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.2907
Hemimycale Arabica Induced Non-Cytotoxic Anti-Migratory Activity in Hepatocellular Carcinoma In Vitro
Hanaa
Rady
National Research Centre, Dokki, Giza, Egypt.
author
Amal
Hassan
National Research Centre, Dokki, Giza, Egypt.
author
Howaida
Abd-Alla
National Research Centre, Dokki, Giza, Egypt.
author
Haiam
Abdel Raouf
National Research Centre, Dokki, Giza, Egypt.
author
Sohair
Salem
National Research Centre, Dokki, Giza, Egypt.
author
text
article
2022
eng
Objective: In this work, we represented new non-cytotoxic treatments to avoid serious side effects of current used cytotoxic anticancer drugs. These treatments can compensate in finding convenient treatment for each individual case using a single agent from marine sponge Hemimycale arabica. Methods: The ethanol extract was partitioned by cold sequential liquid-liquid extraction to afford petroleum ether, diethyl ether, dichloromethane and ethyl acetate fractions. Chemical composition of H. arabica was performed by gas-liquid chromatography and gas chromatography-mass spectroscopy. Anticancer activity was evaluated by means of cytotoxicity, apoptosis induction, tumor cell migration inhibition and expression analysis of proliferation and migration-related genes. Results: Our results revealed that all treatments were non-cytotoxic except for dichloromethane fraction which exhibited moderate cytotoxic activity. Caspase-independent apoptosis was induced by total ethanol and dichloromethane fractions while ethyl acetate fraction induces caspase-dependent apoptosis. All treatments inhibited matrix metalloproteinase-independent migration. Petroleum ether and dichloromethane inhibited migration through the down-regulation of FGF and it could be used as anticancer therapy for VEGF-resistance patients. While ethanol inhibited tumor cell migration through down-regulation of all tested genes expression. Ether and ethyl acetate fractions exerted anti-migratory activity without affecting the tested genes. All resuls were statistically significant at p˂0.05. Conclusion: Total ethanol extract is a promising non-cytotoxic anticancer agent because of its powerful apoptosis induction and capability to block tumor cell migration. Petroleum ether and ether fractions area weak non-cytotoxic anti-migratory agents. Dichloromethane could be a moderate cytotoxic anti-migratory agent induced caspase-independent apoptosis. It could be used in anticancer therapy for VEGF-resistance patients through downregulation of FGF. Ethyl acetate fraction considered a non-cytotoxic agent exerting moderate anti-migratory activity. The new sponge-derived treatments can solve different resistance problems to find a convenient treatment for each individual case using a single agent.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
2921
2928
https://journal.waocp.org/article_90297_cd14664cab5fde7df88392bb009b680c.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.2921
The Risk of Oral Cancer among Different Categories of Exposure to Tobacco Smoking in Sri Lanka
Sajith
Edirisinghe
Department of Anatomy, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka.
author
Manjula
Weerasekera
Department of Microbiology, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri
Lanka.
author
Dulmini
De Silva
Department of Anatomy, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka.
author
Indunil
Liyanage
Department of Anatomy, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka.
author
Malith
Niluka
Department of Anatomy, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka.
author
Kasuni
Madushika
Department of Anatomy, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka.
author
Sandeepani
Deegodagamage
Department of Anatomy, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka.
author
Chanuka
Wijesundara
Department of Anatomy, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka.
author
Alison
Rich
Department of Oral Diagnostic and Surgical Sciences, Faculty of Dentistry, University of Otago, Dunedin, New Zealand.
author
Harsha
De Silva
Department of Oral Diagnostic and Surgical Sciences, Faculty of Dentistry, University of Otago, Dunedin, New Zealand.
author
Haizal
Hussaini
Department of Oral Diagnostic and Surgical Sciences, Faculty of Dentistry, University of Otago, Dunedin, New Zealand.
author
Kanishka
De Silva
National Cancer Institute of Sri Lanka, Apeksha Hospital, Maharagama, Sri Lanka.
author
Surangi
Yasawardene
Department of Anatomy, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka.
author
text
article
2022
eng
Background: The global incidence of oral squamous cell carcinoma (OSCC) is on the rise with no improvement seen in survival rates. Tobacco consumption varies depending on geographic location, ethnicity and culture. The present case-controlled study aimed to determine the relative risk of OSCC for different tobacco consumption patterns in a selected Sri Lankan population. Methods: One hundred and five patients with histopathologically confirmed OSCC attending the National Cancer Institute (Apeksha Hospital) of Sri Lanka and 210 age and gender-matched controls from the community responded to an interviewer-administered questionnaire regarding their smoking and betel-quid chewing (with/ without smokeless tobacco) habits were included in the study. The odds ratios (OR) and 95% confidence intervals (CI) were calculated. p<0.05 was considered as statistically significant. Results: The overall risk of OSCC increased 2.93-fold for smokers. Those smoking two packets of cigarettes or more per day (OR=5.56; 95% CI-2.822-10.984; p=0.000) had more than double the risk of OSCC than those smoking 1-2 packets per day. Smoking for more than 20 years had a 3.4-fold risk of OSCC. Consumption of betel quid containing tobacco (smokeless tobacco) had a 4.26-fold higher risk for OSCC (OR=4.26; 95% CI-2.21-8.21; p=0.000), and the risk increased when all four ingredients (betel leaf, slaked lime, areca nut, and tobacco) were consumed together (OR=4.26; 95% CI-2.34-7.74; p=0.000). The combined effect from concurrent smoking and betel chewing emerged as the highest risk for OSCC (OR=15.34) which significantly exceeded the risks evident for the two habits practised in isolation from each other. Conclusions: Use of smokeless tobacco, consumption of all four ingredients together, duration of smoking, the number of cigarettes smoked per day and combined consumption of betel quid and smoking are significant risk factors in the development of OSCC among Sri Lankans.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
2929
2935
https://journal.waocp.org/article_90290_dec3057ae3feb3382e834b1a39ef8696.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.2929
Serum Levels of Interleukin-1β and Disease Progression in Multiple Myeloma Patients: A Case and Control Study
Shirin
Teimuri Nobari
Department of Biochemistry, Faculty of Medical Sciences, Urmia University of Medical Sciences, Urmia, Iran.
author
Yousef
Rasmi
Department of Biochemistry, Faculty of Medical Sciences, Urmia University of Medical Sciences, Urmia, Iran.
author
Mohammad Hassan
Khadem Ansari
Department of Biochemistry, Faculty of Medical Sciences, Urmia University of Medical Sciences, Urmia, Iran.
author
text
article
2022
eng
Objective: Multiple myeloma (MM) is known as an incurable heterogeneous plasma cell malignancy that presents with a variety of clinical manifestations. Inflammation plays an important role in this disease. Cytokines and Chemokines cause the progression of the disease. One of them is interleukin-1β (IL-1β), which may be involved in the pathogenesis of MM. Other markers such as calcium, albumin, creatinine, globulins, and total protein are also used to diagnose and prognosis patients. The main purpose of this study was to evaluate the serum level of IL-1β and various forms of calcium (total calcium, ionized calcium, and corrected calcium), albumin, creatinine, globulin, and total protein on stage-I of MM patients and healthy controls. Methods: Serum samples from 30 stage-I MM patients and 30 healthy subjects as controls were examined in this study. The protein concentrations of serum IL-1β was assessed by enzyme-linked immunosorbent assay (ELISA), total calcium, albumin, creatinine, total protein, and globulin Measured by auto analyzer BT3000, an electrolyte analyzer was used to measure ionized calcium (Ca++) and a special equation was used to calculate the corrected calcium. Result: The mean level of IL-1β was significantly elevated in stage-I MM. The mean levels of IL-1β were 7.04±1.15 ng/ml in stage-I MM and 3.12± 0.90 ng/ml in controls (p<0.001). The mean levels of total calcium (total Ca) were 9.45±0.56 mg/dl in stage-I MM and 9.09±0.43mg/dl in controls (p=0.008). The mean levels of ionized calcium (Ca++) was 4.65±0.28mg/dl in stage-I MM and 4.75±0.33mg/dl in controls (p=0.2). The mean ratio of serum ionized calcium to total calcium (Ca++/ total Ca) was 0.49±0.054 in stage-I MM and 0.52±0.047 in controls (p=0.02). The mean ratio of serum ionized calcium to corrected calcium (Ca++/corrected Ca) was 0.42±0.033 in stage-I MM and the Mean ratio of serum ionized calcium to calcium total (Ca++/ total Ca) was 0.52±0.047 in controls, Comparison of the mean of the two groups shows a significant difference (p<0.001). The mean level of albumin was 1.72±0.35 g/dl in stage-I MM and4.32±0.41g/dl in controls (p<0.001). The mean level of total protein was 12.65±0.81g/dl in stage-I MM and 7.07±0.4 g/dl in controls (p<0.001). The mean level of globulin was 11.00±0.96 mg/dl in stage-I MM and 2.85±0.77 mg/dl in controls (p<0.001). The mean level of creatinine was 1.15±0.25 mg/dl in stage-I MM and 0.96±0.15 mg/dl in controls (p=0.001). Conclusion: The results of the study indicate the possible involvement of IL-1β at stage-I MM and it can indicate the role of chemokines in the disease process, especially in the early stages. Changes in the chemical profiles mentioned can help in the diagnosis and prognosis of the disease.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
2937
2942
https://journal.waocp.org/article_90263_124a3a433763f29aafa1eed7c0262ae9.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.2937
The Anticancer Effects of Novel Imidazo[1,2-a]Pyridine Compounds against HCC1937 Breast Cancer Cells
Akram
Altaher
Department of Medical Sciences, University College of Science and Technology-Khan Yunis, Gaza Strip, Palestine.
author
Mohammed
Adris
Department
of Biochemistry, Faculty of Medicine, University of AL-Butana, Ruffaa, Sudan.
author
Saeb
Aliwaini
Department of Biology and Biotechnology, Islamic
University of Gaza, Gaza PO Box 108, Palestine.
author
Adel
Awadallah
Department of Chemistry, Faculty of Sciences, Islamic University of Gaza, Gaza
PO Box 108, Palestine.
author
Rami
Morjan
Department of Chemistry, Faculty of Sciences, Islamic University of Gaza, Gaza
PO Box 108, Palestine.
author
text
article
2022
eng
Background: Anticancer drugs confront clinical obstacles such as drug resistance and adverse effects. Imidazo[1,2-a]pyridines (IPs) compounds have lately gained considerable interest as possible anticancer therapeutics due to their potent inhibitory function against cancers cells. This study was to determine the anticancer activities of three novel IPs (IP-5, IP-6, and IP-7) against the HCC1937 breast cancer cell line in vitro. Materials and Methods: The cytotoxic and anti-proliferative effects of IPs compounds in HCC1937 cells were determined by cell viability (MTT) assay, trypan blue assay, and clonogenic survival assay. Scratch motility assay was used to test the antimigration ability of the IPs. Western blot analysis was carried out to detect the level of apoptosis and cell cycle protein markers and to understand the mechanism of action of IPs compounds. Results: IP-5 and IP-6 have a strong cytotoxic impact against HCC1937 cells with IC50 values of 45µM and 47.7µM respectively. IP-7 possesses less cytotoxic effect against HCC1937 cells with IC50 of 79.6µM. Trypan blue assay showed that the three compounds induce significant cell death in the HCC1937 cells. Clonogenic and mammosphere assays demonstrated that IP-5 reduced the HCC1937 cells survival rate by more than 25.0% at 1000 cell concentrations. Western blotting analysis showed that IP-5 compound causes cell cycle arrest as noted by the increasing levels of p53 and p21 in treated cells. IP-5 induced an extrinsic apoptosis pathway as reveals from the increased activity of caspase 7, caspase 8, and the increasing level of PARP cleavage in treated cells. Also, IP-5 treated cells revealed segmented chromatin which is characteristic of apoptotic cells as shown by DAPI stain. Importantly, In comparison to control cells, IP-5-treated cells exhibited lower levels of pAKT. Conclusions: The novel three IPs compounds represent potential active anticancer compounds against HCC1937 breast cancer cells in vitro.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
2943
2951
https://journal.waocp.org/article_90265_ef924252229db6df41e8601b86293b8c.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.2943
Invasion and Metastasis Suppression by Anti-Neonatal Nav1.5 Antibodies in Breast Cancer
Nur
Sharudin
Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Health Campus, 16150 Kubang Kerian,
Kelantan, Malaysia.
author
Ahmad
Murtadha Noor Din
Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Health Campus, 16150 Kubang Kerian,
Kelantan, Malaysia.
author
Irfan
Azahar
Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Health Campus, 16150 Kubang Kerian,
Kelantan, Malaysia.
author
Mawaddah
Mohd Azlan
Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Health Campus, 16150 Kubang Kerian,
Kelantan, Malaysia.
author
Nik
Yaacob
Department of Chemical Pathology, School of Medical Sciences, Universiti Sains Malaysia, Health Campus,
16150 Kubang Kerian, Kelantan, Malaysia.
author
Maria
Sarmiento
School of Health Sciences, Universiti Sains Malaysia, Health Campus, 16150 Kubang
Kerian, Kelantan, Malaysia.
author
Armando
Dominguez
School of Health Sciences, Universiti Sains Malaysia, Health Campus, 16150 Kubang
Kerian, Kelantan, Malaysia.
author
Noor Fatmawati
Mokhtar
Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Health Campus, 16150 Kubang Kerian,
Kelantan, Malaysia.
author
text
article
2022
eng
Background: Detectable neonatal Nav1.5 (nNav1.5) expression in tumour breast tissue positive for lymph node metastasis and triple-negative subtype serves as a valid tumour-associated antigen to target and prevent breast cancer invasion and metastasis. Therapeutic antibodies against tumour antigens have become the predominant class of new drugs in cancer therapy because of their fewer adverse effects and high specificity. Objective: This study was designed to investigate the therapeutic and anti-metastatic potential of the two newly obtained anti-nNav1.5 antibodies, polyclonal anti-nNav1.5 (pAb-nNav1.5) and monoclonal anti-nNav1.5 (mAb-nNav1.5), on breast cancer invasion and metastasis. Methods: MDA-MB-231 and 4T1 cells were used as in vitro models to study the effect of pAb-nNav1.5 (59.2 µg/ml) and mAb-nNav1.5 (10 µg/ml) (24 hours treatment) on cell invasion. 4T1-induced mammary tumours in BALB/c female mice were used as an in vivo model to study the effect of a single dose of intravenous pAb-nNav1.5 (1 mg/ml) and mAb-nNav1.5 (1 mg/ml) on the occurrence of metastasis. Real-time PCR and immunofluorescence staining were conducted to assess the effect of antibody treatment on nNav1.5 mRNA and protein expression, respectively. The animals’ body weight, organs, lesions, and tumour mass were also measured and compared. Results: pAb-nNav1.5 and mAb-nNav1.5 treatments effectively suppressed the invasion of MDA-MB-231 and 4T1 cells in the 3D spheroid invasion assay. Both antibodies significantly reduced nNav1.5 gene and protein expression in these cell lines. Treatment with pAb-nNav1.5 and mAb-nNav1.5 successfully reduced mammary tumour tissue size and mass and prevented lesions in vital organs of the mammary tumour animal model whilst maintaining the animal’s healthy weight. mRNA expression of nNav1.5 in mammary tumour tissues was only reduced by mAb-nNav1.5. Conclusion: Overall, this work verifies the uniqueness of targeting nNav1.5 in breast cancer invasion and metastasis prevention, but more importantly, humanised versions of mAb-nNav1.5 may be valuable passive immunotherapeutic agents to target nNav1.5 in breast cancer.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
2953
2964
https://journal.waocp.org/article_90291_277b77cc565ea8d5e899edce529edff2.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.2953
Detection of Occult Hepatitis C Virus Infection in Egyptian Patients Who Achieved a Sustained Virologic Response to Direct-Acting Antiviral Agents
Ahmed
Mashaal
Hepatogastroentrology, Theodor Bilharz Research Institute, Egypt.
author
Mohamed
Abd El‐Hameed
Hepatogastroentrology, Theodor Bilharz Research Institute, Egypt.
author
Ahmed
El Ray
Hepatogastroentrology, Theodor Bilharz Research Institute, Egypt.
author
Tarek
Mahmoud Diab
Department of Immunoparasitology, Theodor Bilharz Research Institute, Egypt.
author
Reham
Dawood
Department
of Microbial Biotechnology, Biotechnology Research Institute, National Research Centre, Egypt.
author
Mohamed
Shemis
Department of Biochemistry & Mol. Biology, Theodor
Bilharz Research Institute, Egypt.
author
Moataz
Seyam
Hepatogastroentrology, Theodor Bilharz Research Institute, Egypt.
author
text
article
2022
eng
Background: Occult hepatitis C virus (HCV) infection (OCI) is diagnosed based on the detection of HCV-RNA in non-serum reservoirs, such as peripheral blood mononuclear cells (PBMCs) and/or hepatocytes with undetectable HCV-RNA in the serum. The current study was designed to shed more light on the presence of occult HCV in a population of cases who achieved an SVR after receiving treatments for HCV-infection and its significance. Methods: This cross-sectional study evaluated 111 chronic HCV patients treated at Theodor Bilharz Research Institute, Egypt and achieved a sustained virological response (SVR) 12 -24 weeks after treatment with Direct acting antiviral drugs (DAAs). The treatment lasted 12 or 24 weeks using generic medications including Sofosbuvir (SOF) 400 mg/day and Daclatasvir (DCV) 60 mg/day ± weight-based Ribavirin (RBV) 600-1000 mg/day. After achieving the SVR 12 -24 weeks, all patients were subjected to clinical examination and full laboratory investigations. All the candidates were assessed for fibrosis pre/post-treatment by transient elastography (Fibroscan©). Eighty-seven patients (78.4%) received dual therapy (SOF/DCV) and 24 patients (21.6%) received triple therapy (SOF/DCV/RBV). One hundred and seven patients received the regimen for 12 weeks (96.4%) and only four patients received the regimen for 24 weeks (3.6%). All patients were examined in terms of HCV RNA in plasma and PBMCs. Results: Nine patients (8.1%) were positive for PBMCs HCV RNA. The presence of Occult HCV infection (OCI) was significantly correlated with age, level of AFP, and the degree of liver stiffness. Conclusion: The OCI was present in 8.1% of the patients who achieved an SVR 12 – 24 weeks. These patients were mostly aged and with elevated AFP and advanced fibrosis. Monitoring and follow-up of those patients may help to assess the outcomes.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
2965
2971
https://journal.waocp.org/article_90292_eb915e953c12f6b926ae0bb0775c887a.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.2965
Estimation of Surface Dose in the Presence of Unwanted Air Gaps under the Bolus in Postmastectomy Radiation Therapy: A Phantom Dosimetric Study
Lobo
Dilson
Department of Radiation Oncology, Kasturba Medical College (A Constituent Institution of Manipal Academy of Higher Education),
Mangalore, Karnataka, India.
author
Srinivas
Challapalli
Department of Radiation Oncology, Kasturba Medical College, Mangalore , Manipal Academy of Higher Education, Manipal, India.
author
Banerjee
Sourjya
Department of Radiation Oncology, Kasturba Medical College (A Constituent Institution of Manipal Academy of Higher Education),
Mangalore, Karnataka, India.
author
M S
Athiyamaan
Department of Radiation Oncology, Kasturba Medical College (A Constituent Institution of Manipal Academy of Higher Education),
Mangalore, Karnataka, India.
author
Ravichandran
Ramamoorthy
Department of Radiation Oncology, Kasturba Medical College (A Constituent Institution of Manipal Academy of Higher Education),
Mangalore, Karnataka, India.
author
Sunny
Johan
Department of Radiation Oncology, Kasturba Medical College (A Constituent Institution of Manipal Academy of Higher Education),
Mangalore, Karnataka, India.
author
Krishna
Abhishek
Department of Radiation Oncology, Kasturba Medical College (A Constituent Institution of Manipal Academy of Higher Education),
Mangalore, Karnataka, India.
author
text
article
2022
eng
Background: The aim of this study is to design and fabricate a thorax phantom with irregularly shaped trapezoidal slots across the left side of the chest wall, allowing for the creation of unwanted air gaps under the bolus. Method: Surface dose (Dsurf) measurements were made with Gaf Chromic EBT3 films at air gaps (0.0, 5.0, 10.0 and 15.0 mm) under gel bolus of thickness (5.0 mm & 10.0 mm), for 3DCRT technique (2 and 3 field) with clinical 6 MV photon beam under uniform and non-uniform air gap condition. The obtained values were compared with TPS estimated ones. Results: In the presence of 15.0 mm uniform air gap, the mean estimated and measured Dsurf values with two and three field techniques decreased by 14.0 % to 15.2% and 14.7% to 17.4% under 5.0 mm and 10.0 mm bolus applications respectively. In presence of non-uniform air gap condition, the effect on Dsurf was minimal (3 to 3.5%) compared with the uniform air gap condition. Conclusions: Based on the study’s findings, it is recommended that when using bolus in clinical radiotherapy applications, special care be taken to avoid unwanted air gaps under the bolus in order to achieve a uniform surface dose across the treatment region, where a customized 3D printed bolus may be a better option.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
2973
2981
https://journal.waocp.org/article_90266_36eb6989576ec956abe1c7bd18f8f67f.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.2973
Evaluation of TS and ENOSF1 Variants as a Biomarker in Response to Neoadjuvant Chemotherapy based on 5FU in Gastric Cancer Patients
Khadijeh
Arjmandi
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
author
Fereshteh
Ameli
Department of Pathology, Cancer
Institute, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran.
author
Iman
Salahshourifar
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
author
mhsen
Esfandbod
Department of Hematology and
Oncology, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences TUMS, Tehran, Iran.
author
Shiva
Irani
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
author
text
article
2022
eng
Objective: Neoadjuvant chemotherapy with 5-fluorouracil (5FU) is one of the most effective treatment options for gastric cancer patients. However, treatment response varies significantly between patients based on their genetic profile. The purpose of this study was to determine the association between thymidylate synthase (TS) and enolase superfamily member 1 (ENOSF1) polymorphisms, treatment response, and overall survival in patients with gastric cancer. Methods: The TS and ENOSF1 variants were analyzed in formalin-fixed paraffin-embedded (FFPE) tissue from 100 gastric cancer patients receiving neoadjuvant 5FU-based chemotherapy. Polymerase chain reaction (PCR) amplification and restriction fragment length polymorphism (RFLP) were used to determine TS polymorphisms’ genotypes, and the Tetra Arms PCR method was used to identify ENOSF1 polymorphisms. Patients were followed for up to five years, and the association between variants, treatment response, and overall survival (OS) was examined. Results: There was a significant association between the TS 5’ UTR polymorphism and response to treatment in patients with gastric cancer who received neoadjuvant 5FU therapy (P=0.032). Patients with the 2R3R genotype responded better to treatment, whereas those with the 3R3R genotype did not respond to treatment. Patients with the 2R2R and 3R3R genotypes had the longest and shortest median survival times, respectively, and the observed differences were significant (p=0.003). There was a statistically significant relationship between rs2612091 and chemotherapy response (P=0.017). Patients with genotype AG did not respond to treatment. Conclusion: This study established that the TS 5’ UTR and ENOSF1 rs2612091 polymorphisms could be used to predict treatment response and overall survival in patients with gastric cancer who received neoadjuvant chemotherapy based on 5FU.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
2983
2989
https://journal.waocp.org/article_90280_41b6c76968b7c5346b916d68ae40dab9.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.2983
Adolescents and E-cigarettes in India: A Qualitative Study of Perceptions and Practices
Himanshu
Gupte
Narotam Sekhsaria Foundation, Mumbai, India.
author
Nilesh
Chatterjee
Narotam Sekhsaria Foundation, Mumbai, India.
author
Gauri
Mandal
Salaam Bombay Foundation, Mumbai, India.
author
Marina
D’Costa
Narotam Sekhsaria Foundation, Mumbai, India.
author
text
article
2022
eng
Purpose: Global Youth Tobacco Survey-4, India conducted in 2019 showed ‘ever use’ of e-cigarettes among adolescents to be 2.8%. However, there is dearth of qualitative data on adolescent use of e-cigarettes in the country. This study was conducted to explore and gain better understanding on adolescents’ perceptions and practices about e-cigarette use. Methods: In-depth interviews were conducted with 24 adolescents who self-reported use of e-cigarettes. The participants were recruited from ten municipal schools of Mumbai, India that cater to students from lower socio-economic background. Participants were from 7th to 9th grades, and aged 11-16 years. Data from in-depth interviews were analyzed using inductive thematic analysis. Results: Adolescents referred to ‘e-cigarette’ as ‘pen-hookah.’ E-cigarettes were perceived as relatively harmless compared to regular hookahs and conventional cigarettes. Initiation was influenced by a friend, peer, or sibling. A variety of flavors, the after-taste, the ability to perform playful tricks with smoke, and fun-time spent with friends were cited as reasons for continued use. Social media influenced both initiation and continuation. Most adolescents’ regular use was with a group of friends; the device was shared with or obtained from friends or siblings. Adolescents were unclear about the presence of nicotine in refill liquids and the harmful health effects. Conclusion: Increasing awareness among adolescents about the harms of e-cigarettes is urgently required through comprehensive tobacco-prevention programs. More research is needed to examine the role of flavors in increasing acceptability of e-cigarettes and how it affects perceived harmfulness of tobacco products.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
2991
2997
https://journal.waocp.org/article_90267_36ff3815b5167ee56294649861560279.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.2991
Generation and Characterization of Novel Diagnostic Mouse Monoclonal Antibodies Against Human Estrogen Receptor Alpha and Progesterone Receptor
Masoud
Hassanzadeh Makoui
Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
author
Maryam
Mobini
Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
author
Jalal
Khoshnoodi
Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
author
Mohammad Ali
Judaki
Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
author
Tannaz
Bahadori
Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
author
Hengameh
Ahmadi Zare
Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
author
Forough
Golsaz-Shirazi
Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
author
Hediyeh
Moradi Tabriz
Department of Pathology, Tehran University of Medical Sciences, Tehran, Iran.
author
Zahra
Madjd
Oncopathology Research Center, Iran University of Medical Sciences (IUMS), Tehran, Iran.
author
Mahmood
Jeddi-Tehrani
Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
author
Amir-Hassan
Zarnani
Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
author
Mohammad Mehdi
Amiri
Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
author
Fazel
Shokri
Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
author
text
article
2022
eng
Background: Estrogen and progesterone regulate the growth and development of several human cells and tissues. Their corresponding receptors (ER and PR) are important diagnostic and prognostic indicators for cancers of the breast and reproductive organs. Immunohistochemical analysis of ER and PR is the current standard method for evaluating the expression of these receptors in different cancers. Nonetheless, there is a significant lack of reproducibility of IHC results in laboratories worldwide, necessitating to develop more sensitive and specific antibodies for ER and PR IHC staining. Methods: ER and PR-specific monoclonal antibodies (MAbs) were generated by immunizing mice with synthetic peptides from ERα and PR. The isotypes and affinity constants of the selected MAbs were determined, and their specificities were assessed by peptide-specific ELISA, IHC, Western-blot analysis, and flow cytometry. In addition, the reactivity of generated MAbs was compared with that of the commercially-available anti-ER and anti-PR antibodies in IHC using normal and cancerous tissue sections. Moreover, 200 breast cancer tissue samples were stained using the newly generated MAbs along with commercial antibodies by IHC, and the sensitivity, specificity and accuracy of our MAbs were evaluated. Results: Among different MAbs generated in this study, two anti-ER and one anti-PR MAbs specifically detected the target antigens in normal and cancerous tissues in IHC. Further analyses confirmed the specificity of the MAbs in Western blotting and flow cytometry using a panel of ER and PR positive cell lines. The sensitivity, specificity and accuracy calculated for clone 1B9 (anti-ER) were 92.3%, 94.8% and 93%, and for clone 3D6 (anti-PR) were 93.0%, 94.3% and 93.5%, respectively. Conclusion: Our novel anti-ER and PR MAbs could be considered as suitable tools for diagnostic and research purposes.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
2999
3007
https://journal.waocp.org/article_90268_0b3369a0da3b188e22f9f035193e2477.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.2999
Plan Quality Index – An Integrated Dosimetric Approach for Plan Evaluation with Consideration of Quality of Dose Coverage to Tumors and Quality of Organs’ Sparing
Jayapalan
Krishnan
Department of Radiation Oncology, Yenepoya Medical College Hospital, Mangaluru, India.
author
Suresh
Rao
Department of Radiation Oncology,
Mangalore Institute of Oncology, Mangaluru, India.
author
Sanath
Hegde
Department of Radiation Oncology,
Mangalore Institute of Oncology, Mangaluru, India.
author
text
article
2022
eng
Purpose: To find an integrated solution for plan evaluation with multiple dosimetric parameters in order to ensure quality of target dose coverage and sparing of organs collectively. Materials and Methods: A mathematical logical expression called Plan Quality Index (PQI) was formulated. It includes two integrated indices 1.Integrated Dosimetric Index for tumor (IDITumor) dose coverage and 2.Integrated Dosimetic Index (IDIorgans) for Organs At Risk (OARs). PQI was used to select better dosimetric plan form multiple VMAT plans of a Nasopharynx case. In addition, the PQI was used for comparison of VMAT and IMRT plans of 64 patients with Head and Neck cancer. The plan with lesser PQI was considered as a better dosimetric plan. For statistical comparison between two techniques, paired‘t’ and Wilcoxon signed rank test were used with consideration p< 0.05 as a statistically significant. Results: The unambiguous evaluation results with PQI showed that VMAT plan was achieved the entire given dose constraints significantly better than IMRT plan in all the patients (p<0.001). Conclusion: Plan evaluation with PQI can be an unambiguous method. It evaluates the quality of tumor dose coverage (IDITumor) and sparing of OARs (IDIorgans) collectively. It enables to change the priority of evaluation criteria of a specified structure based on the clinical requirements. It does not require any specialized program. It can be calculated using ordinary excel program. Using the single value as PQI, the effective determination of a quality plan among many plans can be possible and it can be used for comparison of different techniques.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
3009
3017
https://journal.waocp.org/article_90269_849630151139b56a8102ea39726697fb.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.3009
Health-Related Quality of Life, Functioning, and Physical Symptoms of Adult Omani Colorectal Cancer Survivors
Maryam
Al-Shandudi
Department of Family Medicine and Public Health, College of Medicine and Health Sciences, Sultan Qaboos University, Muscat,
Oman.
author
Mansour
Al-Moundhri
Sultan Qaboos Comprehensive Cancer Care and Research Center, Muscat, Oman.
author
Moon Fai
Chan
Department of Family Medicine and Public Health, College of Medicine and Health Sciences, Sultan Qaboos University, Muscat,
Oman.
author
Thuraya
Al-Hajri
National Oncology Centre, Royal
Hospital, Muscat, Oman.
author
Muna
Al-Balushi
Sultan Qaboos Comprehensive Cancer Care and Research Center, Muscat, Oman.
author
Mohammed
Al-Azri
Department of Family Medicine and Public Health, College of Medicine and Health Sciences, Sultan Qaboos University, Muscat,
Oman.
author
text
article
2022
eng
Background: Colorectal (CRC) survivors often experience physical and psychological symptoms affecting their health-related quality of life (HRQoL). This study aimed to identify factors impacting HRQoL-related functioning and physical symptoms among adult Omani CRC survivors. Methods: A cross-sectional study of 124 adult CRC survivors was conducted at the two main oncology referral hospitals in Oman. A validated Arabic version of the European Organization for Research and Treatment of Cancer-Quality of Life Questionnaire 30 was used to collect data. Results: A total of 118 CRC survivors participated in the study (response rate: 95.2%). The mean age was 52 years and there were an equal number of male and female participants (n = 59 each; 50.0%). A total of 62 survivors (52.5%) had been diagnosed with CRC at stages III or IV. The overall score for global health was high (81.7). With regards to functioning, high mean scores were observed for domains of role (91.0) and social (90.7) functioning. In terms of symptoms, high mean scores were reported for constipation (25.4), insomnia (25.1), pain (20.1), and fatigue (18.9). Survivors under 60 years old (β=15.5, p=.004) and those with no comorbidities (β=16.0, p=.001) demonstrated better functional HRQoL. Being male was predictive of better functional HRQoL in the emotional (β=13.9, p<0.008), cognitive functioning (β=12.5, p=.013), role functioning (β=14.0, p=0.006) and physical functioning (β=17.8, p<0.001) domains. Conclusions: Healthcare professionals in Oman should implement measures to enhance the HRQoL of CRC survivors, particularly women and those with coexisting morbidities.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
3019
3027
https://journal.waocp.org/article_90281_0ecfbe1fb45e4a8097c0b2c13bc88146.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.3019
Home-based Palliative Intervention to Improve Quality of Life in Children with Cancer: A Randomized Controlled Trial
Murti
Andriastuti
Department of Child Health, Faculty of Medicine Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta, Indonesia.
author
Pricilia
Halim
Department of Child Health, Faculty of Medicine Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta, Indonesia.
author
Elnino
Tunjungsari
Pediatric Nursing, Cipto Mangunkusumo Hospital, Jakarta, Indonesia.
author
Dwi
Widodo
Department of Child Health, Faculty of Medicine Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta, Indonesia.
author
text
article
2022
eng
Objective: Over the past few years, an integrated approach of palliative care (PC) to chronic and/or life-threatening conditions care has been widely used. Home-based PC (HBPC) service is developed to meet the needs of patients at home; however, it has not been used widely. This study is aimed at determining the benefits of integrated HBPC for the quality of life (QoL) and symptoms intensity in Indonesian children with malignancies. Method: A randomized controlled trial was carried out to compare the quality of life between patients who were given PC (a three-month home visit) and those who did not receive PC (control group). Each group was constituted of thirty children with cancer and aged 2-18 years old and were consulted by a palliative team. The participants were randomly allocated to two groups. In the first and twelfth weeks of the intervention, all patients were assessed using the Pediatric Quality of Life Inventory (PedsQLTM) questionnaire cancer module 3.0 (report by proxy or self-report). Symptoms intensity (pain, anorexia, sleep disturbance) were scored by using Edmonton Symptoms Assessment Scale (ESAS). The mean score and each dimension score of both groups were compared and analyzed using bivariate analysis. Results: In total, fifty participants were included in the study. A significant difference was found between the two groups in terms of the mean total score in control group 62.39 and intervention group 81.63 (p<0.001). The QoL was improved in the intervention group, while it was declined in the control group as the disease progressed. The main improvements were in the pain and nausea aspects (p<0.001), followed by procedural anxiety (p=0.002), treatment anxiety (p=0.002), and worry (p=0.014). Palliative intervention significantly reduced sleep disturbances (p=0.003) and anorexia (p<0.001). Conclusion: Home-based PC improved several aspects of QoL and caused better symptom management in children with malignancies. An early intervention concurrent with the underlying treatment can improve QoL in these children.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
3029
3034
https://journal.waocp.org/article_90295_b9e57255a96e03f2fe5426bda17af3d7.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.3029
Adherence to Chemotherapy among Women with Breast Cancer Treated at Tikur Anbessa Specialized and Teaching Hospital, Addis Ababa, Ethiopia
Fatuma
Hassen
School of Public Health, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopi.
author
Fikre
Enquselassie
School of Public Health,
College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia.
author
Ahmed
Ali
School of Public Health,
College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia.
author
Adamu
Addissie
School of Public Health,
College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia.
author
Girma
Taye
School of Public Health,
College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia.
author
Mathewos
Assefa
Department of Oncology, School of Medicine,
College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia.
author
Aster
Tsegaye
Department of Medical
Laboratory Sciences, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia.
author
text
article
2022
eng
Background: Adherence is important for women with breast cancer because it is a primary determinant for effectiveness of treatment and optimum clinical benefit. Though Breast cancer is the leading cancer in Ethiopia,adherance to chemotherapy is not investigated in Ethiopian women. Objective: This study aimed to assess adherence to chemotherapy among women with breast cancer treated at Tikur Anbessa specialized and Teaching Hospital. Methods: Cross-sectional study was conducted among 164 breast cancer patients with chemotherapy. After eligible participants were identified, data were collected using face-to-face interviews, card reviews and telephone interviews. Adherence was calculated as the number of doses taken divided by number of recommended or expected doses. Pearson chi-square test was used to evaluate predictors of adherence. Results: Among a total of 164 breast cancer patients, majority, 119, (72.6%) of them were urban residents. The mean age of study participants was 41.99 + 10.9 years. The majority 149, (90.9%) of patients were married. More than half 94, (57.3%) of the women were literate. In this study, 137 out of 164 (83.5%) women were adherent to their chemotherapy. Of the 27 non adherent participants. he reason for non-adherence to chemotherapy was unknown for 7, (25.9%) of women. Among different identified reasons for non-adherent, sever illness prevents patients to receive chemotherapy. Based on Pearson chi square test, distance from referral center and treatment regimen were significantly associated with non-adherence rate. Conclusion: The present study the results showed that the majority 137, (83.5%) of patients were in good adherence to their chemotherapy. The most identified factor of non-adherence was inability to come for their therapy as a result of severity of illness. Therefore, expansion of cancer diagnosis and treatment centers should be encouraged in order to maximize patient’s access and adherence to chemotherapy.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
3035
3041
https://journal.waocp.org/article_90282_699852e51f162e5821b0222d54a5b292.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.3035
Cervical Cancer Prevention on Instagram: Content and Social Interaction Analysis of Brazilian Accounts
Elen
Vicente
Postgraduate Program in Health Promotion, Universidade Cesumar, Brazil.
author
Sergio Eduardo Emydgio
de Faria
Postgraduate Program in Health Promotion, Universidade Cesumar, Brazil.
author
Ana Beatriz
Almeida
Faculty of Medicine, Cesumar University, Brazil.
author
Patricia Ayumi
Yamada
Faculty of Medicine, Cesumar University, Brazil.
author
Tiago
Lucena
Faculty of Communication and Multimedia, State University of Maringá, Brazil.
author
Tania
Silva
Postgraduate Program in Health Promotion, Universidade Cesumar, Brazil.
author
Marcelo
Bernuci
Postgraduate Program in Health Promotion, Universidade Cesumar, Brazil.
author
text
article
2022
eng
Abstract: Objective: The aim of the present study was to analyse the content of posts on Instagram about cervical cancer. Methods: It was conducted a qualitative analysis using the 50 most popular publicly available Portuguese-language Instagram posts, containing the hashtags #cervicalcancer, #papsmear, #hpv, #papillomavirus, and #hpvvac-cine, during the Brazilian national cervical cancer prevention campaign in March 2018. Results: Posts recruited using #cervicalcancer provided 60% of posts with contents related to secondary prevention; the #papsmear provided 46% of posts with irrelevant contents; the #hpv and #papillomavirus provided 50% and 64% of posts with informative content, respectively; and the #hpvvaccine provided 58% of posts with content related to primary prevention. The posts that received the highest number of likes were those from the hashtags #hpv and #papillomavirus with 151.33 and 78.00 likes/post, respectively. The majority of posts presented less than 05 comments/post, except for the #hpv, which had 64.76 comments/post. According to the users’ profiles, the majority of the posts, regardless of the hashtag used, were made by health professionals. Conclusion: The focus of Instagram posts about cervical cancer is on secondary prevention, which can contribute to the promotion of health behaviours not directed to aspects of primary prevention of the disease.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
3043
3049
https://journal.waocp.org/article_90283_152d02ac58dca5db65254a270a0efe6f.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.3043
Expression of CD133 Cancer Stem Cell Marker in IDH-Mutant and IDH-wildtype (Isocitrate Dehydrogenase) Astrocytoma
Olivia
Sabunga
Department of Pathology, Faculty of Medicine, Hasanuddin University, Indonesia.
author
Cahyono
Kaelan
Department of Pathology, Faculty of Medicine, Hasanuddin University, Indonesia.
author
Andi
Zainuddin
Department of Public Health, Faculty of Medicine, Hasanuddin University, Indonesia.
author
Ni
Sungowati
Department of Pathology, Faculty of Medicine, Hasanuddin University, Indonesia.
author
Muhammad Husni
Cangara
Department of Pathology, Faculty of Medicine, Hasanuddin University, Indonesia.
author
Upik
Miskad
Department of Pathology, Faculty of Medicine, Hasanuddin University, Indonesia.
author
text
article
2022
eng
Objective: This study evaluated the differences between IDH1-R132H and CD133 expression in different categories of astrocytoma. Material and methods: This study used a cross-sectional design. Sixty-seven paraffin embedded block of Diffuse Astrocytoma (DA), Anaplastic Astrocytoma (AA) and Glioblastoma (GB) were assessed using using the monoclonal antibody IDH1-R132H and Rabbit polyclonal antibody CD133. Results: It was found that there was a significant relationship between the expression of IDH1-R132H and CD133 in DA, AA and GB (p<0.001). Astrocytoma with IDH-mutant molecular status will express more markers of cancer stem cell CD133 than IDH-wildtype. Conclusion: The IDH1-R132H and CD133 can provide predictive value on treatment success, disease prognosis, recurrence and can be considered as target combination therapy with chemotherapy.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
3051
3059
https://journal.waocp.org/article_90270_f3c271070823b2e450d03548c3044ad0.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.3051
Pharmacogenetic Study of the Dihydropyridine Dehydrogenase Gene in Jordanian Patients with Colorectal Cancer
Nusaiba
Almashagbah
Department of Biotechnology and Genetic Engineering, Jordan University of Science and Technology, Irbid, Jordan.
author
Amjad
Mahasneh
Department of Biotechnology and Genetic Engineering, Jordan University of Science and Technology, Irbid, Jordan.
author
khaldon
Bodoor
Department of Biotechnology and Genetic Engineering, Jordan University of Science and Technology, Irbid, Jordan.
author
text
article
2022
eng
Introduction: Several studies have shown an association between 5-fluorouracil toxicity and variations in the dihydropyrimidine dehydrogenase (DPYD) gene. Objectives: This cross-sectional study aims to elucidate the association between genetic variations in the DPYD gene and 5-fluorouracil toxicity among Jordanians with colorectal cancer (CRC). Methods: 80 CRC Patients were recruited to screen for mutations in the DPYD gene using the Sanger sequencing technique. Sequencing results were analyzed using Mutation Surveyor software, and mutational effects were predicted by the Mutation Tester bioinformatics tool. Results: Three reported variants (c.85T>C, c.1740+40A>G, c.1740+39C>T) and one novel (g.97515583_97515584insA) variant were identified in this study. Results showed a significant association between these variants and toxicity to 5-Fluorouracil with P-values 0.002, 0.005, 0.019, 0.017, respectively. However, there was no significant association between variants and cancer free survival. Conclusion: The present study identified several variants in the DPYD gene among Jordanians with colorectal cancer, which are associated with toxicity to 5-Fluorouracil treatment.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
3061
3069
https://journal.waocp.org/article_90271_7519ecb118f1d464bb751c294b801090.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.3061
FOXD1-mTOR Signaling Pathway on Oral Squamous Cell Carcinoma and Its Inhibition by Rosemary Extract (Invitro-Study)
Yasmine
Alaa El-din
Oral and Maxillofacial Pathology, Faculty of Dentistry, October 6 University, Cairo, Egypt.
author
Dina
Sabry
Medical Biochemistry and Molecular
Biology, Faculty of Medicine-Cairo University, Cairo, Egypt.
author
Sahar
H. Ahmed
Department of Lab Technology, Faculty of Applied Medical Science, Misr University for Science
andTechnology, Egypt.
author
Abbas
Mohamed
Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Giza,
Egypt.
author
text
article
2022
eng
Background: FOXD1 expression in oral squamous cell carcinoma remains uncovered. The aim was to detect the anticancer effect of Rosemary Extract RE through the evaluation of FOXD1 gene expression in (OSCC) by quantitative PCR. Methods: OSCC cell line was served as a control group. Moreover, the OSCC cell line (SCC-15) was treated with RE (OSCC/ RE group) at 24, 48, and 72 hs time intervals. We assessed the antioxidant activity of RE by evaluation of lipid peroxidation (MDA) and superoxide dismutase (SOD) levels. The cytotoxic effects of RE were examined by MTT assay. mTOR and LC3 I/II autophagy protein markers were assessed by western blot. Apoptosis activity was assessed. Results: The study results were statistically assessed. Intergroup comparisons were analyzed, whereas intragroup comparisons were conducted utilizing one-way repeated measures ANOVA, followed by multiple pairwise paired t-tests with Bonferroni correction revealed a significant increase of FOXD1 gene expression in the control OSCC group in comparison to the OSCC/RE group (p-value <0.001). A significant decrease of mTOR/LC3I/II proteins expression in the OSCC/RE group compared to the control OSCC group (p-value <0.001). Conclusion: FOXD1 can be considred a diagnostic biomarker for OSCC. RE inhibits autophagy of oral human cancer cells via mTOR/LC3I/II-dependent pathways and decrease caspase -3 apoptotic level.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
3071
3081
https://journal.waocp.org/article_90272_c19f85b971f201bd2469c82b56d91baf.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.3071
A Comparative Study of the Bone Marrow- and Umbilical Cord-Derived Mesenchymal Stem Cells (MSCs) Efficiency on Generating MSC-Educated Macrophages (MEMs)
Mahsa
Shirin
Hematology, Oncology and Stem Cell Transplantation Research Center, Shariati Hospital, Tehran University of Medical Science, Tehran, Iran.
author
Mina
Agharezaeei
Hematology, Oncology and Stem Cell Transplantation Research Center, Shariati Hospital, Tehran University of Medical Science, Tehran, Iran.
author
Shaban
Alizadeh
Department of Hematology, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran.
author
Davood
Bashash
Department of Hematology and Blood Banking, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
author
Fatemeh
Sheikhsaran
Hematology, Oncology and Stem Cell Transplantation Research Center, Shariati Hospital, Tehran University of Medical Science, Tehran, Iran.
author
Bahram
Chahardouli
Hematology, Oncology and Stem Cell Transplantation Research Center, Shariati Hospital, Tehran University of Medical Science, Tehran, Iran.
author
Seied A
Mousavi
Hematology, Oncology and Stem Cell Transplantation Research Center, Shariati Hospital, Tehran University of Medical Science, Tehran, Iran.
author
Mohammad
Ahmadvand
Cell Therapy and Hematopoietic Stem Cell Transplantation Research Center, Research Institute for Oncology, Hematology and Cell Therapy, Tehran University of Medical Sciences, Tehran, Iran.
author
Seyed H
Ghaffari
Hematology, Oncology and Stem Cell Transplantation Research Center, Shariati Hospital, Tehran University of Medical Science, Tehran, Iran.
author
text
article
2022
eng
Background: Mesenchymal stem cells (MSCs) have gained much more attention in cell therapy and regenerative medicine due to their immunosuppressive effects. MSCs have interaction with other immune cells, such as macrophages (MQs). Bone marrow (BM)-derived MSCs can educate MQs toward MSC-educated MQs (MEMs) which possess an anti-inflammatory immunophenotype. Given this and based on the important limitations of BM collection, we hypothesized whether co-culture of MQs with umbilical cord (UC)-derived MSCs can result in the MEM phenotype. Methods: First, isolated monocytes cultured for five days to obtain M0 MQs. Then, they were co-cultured with either BM- or UC-MSCs under direct and indirect conditions. After three days of co-culture, MEM-specific surface markers, as well as the gene expression of inflammatory and anti-inflammatory cytokines, were evaluated. Results: Surface expression of CD163/CD206, as specific markers for M2 MQs, increased in MEMs after co-culture with BM- and UC-derived MSCs, while CD80/CD86 expression (specific markers for M1 MQs) didn’t change significantly. The mRNA expressions of PDL-1 as well as anti-inflammatory cytokines, including IL-6, IL-10, and TGFβ also increased in MEMs after co-culture of UC-MSCs compared to control MQs (p <.05), while the expression of IL-12 was significantly decreased (p<.001). Conclusions: To the best of our knowledge, this study shows for the first time that the co-culture of MQs with UC-derived MSCs efficiently contributes to the generation of MEMs even greater than BM-MSCs; shedding light on the promising potential of UC as an alternative source to educate MQs in vitro.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
3083
3092
https://journal.waocp.org/article_90273_7e5a76d1736b509322f8bdf175d0d689.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.3083
Apoptotic and Anti-metastatic Effects of Atractylodes lancea (Thunb.) DC. in a Hamster Model of Cholangiocarcinoma
Paradon
Sonsomnuek
Chulabhorn International College of Medicine, Thammasat University, Rangsit Center, Klong Luang, Pathum Thani, Thailand.
author
Mayuri
Tarasuk
Center of Excellence in Pharmacology and Molecular Biology of Malaria and Cholangiocarcinoma, Chulabhorn International
College of Medicine, Thammasat University, Rangsit Center, Klong Luang, Pathum Thani, Thailand.
author
Tullayakorn
Plengsuriyakarn
Center of Excellence in Pharmacology and Molecular Biology of Malaria and Cholangiocarcinoma, Chulabhorn International
College of Medicine, Thammasat University, Rangsit Center, Klong Luang, Pathum Thani, Thailand.
author
Kanyarat
Boonprasert
Center of Excellence in Pharmacology and Molecular Biology of Malaria and Cholangiocarcinoma, Chulabhorn International
College of Medicine, Thammasat University, Rangsit Center, Klong Luang, Pathum Thani, Thailand.
author
Kesara
Na-Bangchang
Center of Excellence in Pharmacology and Molecular Biology of Malaria and Cholangiocarcinoma, Chulabhorn International
College of Medicine, Thammasat University, Rangsit Center, Klong Luang, Pathum Thani, Thailand.
author
text
article
2022
eng
Objectives: Cholangiocarcinoma (CCA) is a highly aggressive tumor with a greater risk of distant metastasis. The promising anti-CCA activity and safety profile of Atractylodes lancea (AL) have previously been reported in a series of in vitro, in vivo and clinical studies. The present study investigated the effect of AL extract on apoptosis and metastasis signaling pathways in the Opisthorchis viverrini/dimethylnitrosamine (OV/DMN)-induced CCA hamster model. Materials and Methods: Hamster liver tissues were obtained from the four groups (n = 5 per group), i.e., (i) 5-FU treated CCA (40 µg/mL); (ii) CCA; (iii) AL-treated CCA (5,000 mg/kg), and (iv) normal hamsters. Total RNA was isolated, and the expression levels of apoptosis-related and metastasis-related genes were determined by qRT-PCR analysis. Results: The expression levels of p16, caspase-3, caspase-8, caspase-9, Apaf-1, p53 and Eef1a1 were downregulated, while that of the remaining genes were upregulated in CCA hamsters compared with normal hamsters. AL treatment increased the expression of p16, caspase-9, caspase-3, Apaf-1, p53 and E-cadherin and decreased the expression of cyclin D1, cdk4, Bax, Akt/PKB, Bcl-2, Mfge-8, Lass4, S100A6, TGF-β, Smad-2, Smad-3, Smad-4, MMP-9, and N-cadherin. The expression of Eef1a1 was unchanged. Conclusion: The anti-CCA activity of AL in OV/DMN-induced CCA hamsters could be due to the induction of cell cycle arrest at the G1 phase and activation of the apoptosis pathway, resulting in cancer cell death. The activation of the apoptosis pathway mainly involved the intrinsic pathway (activation of caspase-3 and caspase-9 through p53 and Mfge-8 modulation and downregulation of anti-apoptotic genes Akt and Bcl-2). In addition, AL could also inhibit the canonical TGF-β signaling pathway, MMP-9 and N-cadherin to suppress tumor metastasis.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
3093
3101
https://journal.waocp.org/article_90284_c5c8b8b2be5dbea0c198bd7a12dfb98d.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.3093
A Comprehensive Evaluation of Single Isocenter Multiple Target SRS Plans and the Analytical Relationship between Plan Quality Indices with the Number and Volume of Targets
Hemalatha Priyadarsini
V
Tamil Nadu Medical Services Corporation Ltd, 417, Pantheon road, Egmore, Chennai, Tamil Nadu, India.
author
Murali
R
Department
of Physics, School of Advanced Sciences, Vellore Institute of Technology, Vellore, Tamil Nadu, India.
author
Arunai Nambi Raj
N
Department
of Physics, School of Advanced Sciences, Vellore Institute of Technology, Vellore, Tamil Nadu, India.
author
Saravanan
S
Department of
Radiation Oncology, Government Royapettah Hospital, 1, West Cott road, Royapettah, Chennai, Tamil Nadu, India.
author
text
article
2022
eng
Objective: To study the relationship of plan quality indices with the number and volume of target for 5 to 10 brain metastases treated with LINAC-based Single Isocenter Multiple Target (SIMT) Stereotactic radiosurgery (SRS) planning and to determine the maximum volume of spherical targets treated without exceeding the normal tissue tolerances. Methods: Spherical targets of 5 to 10 numbers per plan, with individual target volumes ranging from 0.025 cc to 11.5 cc, were simulated with randomly drawn planning target volumes (PTVs) within the brain. SIMT SRS plans were generated for the 21 Gy prescription dose with a 6 MV Flattening Filter Free photon beam. Target coverage, organ at risk sparing, plan quality indices, R50%, and gradient measure were studied. Mean brain dose, V12 for Brain minus PTV (BmP), V10, V12, V15, V18, V20, and V24 for brain volume were evaluated. Equations relating the gradient index, mean brain dose, and V12 (BmP) to the given number and volume of the targets were constructed. Results: PTV coverage D98 was 98.77 ± 1.37 %. The mean CIRTOG, QRTOG, HIRTOG, CIP, GI, and R50% of the individual targets were 1.02 ± 0.08, 0.94 ± 0.02, 1.49 ± 0.11, 0.91 ± 0.06, 4.74 ± 2.3, and 4.95 ± 2.67, respectively. The gradient measure achieved was in the range of 0.49 to 1.35 cm. The mean brain dose was in the range of 1.62 to 6.69 Gy. The mean V12 (BmP) per target obtained was 3.85 ± 2.83 cc. Conclusion: Equations relating the number and volume of targets to the gradient measure, mean brain dose, V12 (BmP), and V10-24 can serve as a baseline for multiple brain metastases SIMT planning. The target volume for 5, 6, 7, 8, 9, and 10 targets that can be treated without exceeding V12 (BmP) is 6, 5, 4.7, 4, 3.7, and 3.4 cc, respectively, for the 21 Gy prescription dose.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
3103
3112
https://journal.waocp.org/article_90293_be64065db6f9afef5b0ea6ca89a94b6f.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.3103
A New Complex Design of Fe (II) Isoleucine Dithiocarbamate as a Novel Anticancer and Antivirus against SARSCOV-2 (COVID-19)
Rizal
Irfandi
Doctoral Program, Department of Chemistry, Faculty of Mathematics, and Natural Science, Hasanuddin University Makassar,
Indonesia.
author
Riswandi
Riswandi
Department of Chemistry, Faculty of Mathematics, and Natural Science, Hasanuddin University Makassar,
Indonesia.
author
Indah
Raya
Department of Chemistry, Faculty of Mathematics, and Natural Science, Hasanuddin University Makassar,
Indonesia.
author
Ahyar
Ahmad
Department of Chemistry, Faculty of Mathematics, and Natural Science, Hasanuddin University Makassar, Indonesia.
author
Ahmad
Fudholi
Solar Energy Research Institute, Universiti Kebangsaan Malaysia 43600 UKM Bangi, Selangor, Malaysia.
author
Sulistiani
Jarre
Department of Chemistry, Faculty of Mathematics, and Natural Science, Hasanuddin University Makassar, Indonesia.
author
Dewi Ratih
Tirto Sari
Department of Pharmacy, Faculty of Medical Science, Universitas Ibrahimy, Situbondo, East Java, Indonesia.
author
Santi
Santi
Medical Laboratory Technology, Faculty of Health Technology, Megarezky University, Makassar, Indonesia.
author
Ronald Ivan
Wijaya
Department of Chemistry, Faculty of Mathematics, and Natural Science, Hasanuddin University Makassar, Indonesia.
author
Prihantono
Prihantono
Department of Surgery, Faculty of Medical, Hasanuddin University, Makassar, Indonesia.
author
text
article
2022
eng
Background: This study was carried out to synthesize a new complex of Fe(II) with isoleucine dithiocarbamate ligand and to determine its potential as an anticancer and antiviral agent for SARSCOV-2. Methods: The synthesized complexes were then characterized by UV-vis and FT-IR spectroscopy and their melting points. The value of the conductivity of the complex compound is also determined. Anti-cancer activity was tested in vitro and molecular docking. Its potential as an antiviral against SARSCOV-2 was also carried out by molecular docking. Pharmacokinetics/ADMET properties were also carried out on the complex. Result: Spectral results showed the successful synthesis of Fe(II) isoleucine dithiocarbamate complex. The complex produced UV-vis spectra at 268 and 575 nm, and the IR data at 399–599 cm-1 showed the coordination between the Fe(II) atoms with sulphur, nitrogen and oxygen of the isoleucine dithiocarbamate ligand. Fe(II) isoleucine dithiocarbamate had a cytotoxicity effect on the MCF-7 cell line (IC50 =613 µg/mL). The complex significantly caused morphological changes in the breast cancer cell line, finally leading to cell apoptosis. Conclusion: Cytotoxic test of Fe(II) isoleucine dithiocarbamate showed moderate anticancer activity on MCF-7 cancer cells and showed antiviral activity against SARSCOV-2 by interfering with spike glycoprotein –ACE2 receptors, and inhibiting major proteases and 3Clpro.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
3113
3123
https://journal.waocp.org/article_90274_37438b89c32e753292b0842f704736e0.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.3113
Efficacy of Curcumin in Combination with Intralesional Dexamethasone with Hyaluronidase in the Treatment of Oral Submucous Fibrosis: A Randomized Controlled Trial
Sagar
Adhikari
Department of Oral Medicine and Radiology, College of Dental Surgery, B.P. Koirala Institute of Health Sciences, Dharan, Nepal.
author
Jyotsna
Rimal
Department of Oral Medicine and Radiology, College of Dental Surgery, B.P. Koirala Institute of Health Sciences, Dharan, Nepal.
author
Iccha
Maharjan
Department of Oral Medicine and Radiology, College of Dental Surgery, B.P. Koirala Institute of Health Sciences, Dharan, Nepal.
author
Ashish
Shrestha
Department of Public Health Dentistry, College of Dental Surgery, B.P. Koirala Institute of Health Sciences, Dharan, Nepal.
author
text
article
2022
eng
Objective: The objective of this study was to determine the efficacy of curcumin in combination with intralesional dexamethasone with hyaluronidase in the treatment of oral submucous fibrosis (OSF). Methods: This randomized, double blind, parallel design, clinical trial was conducted at B.P. Koirala Institute of Health Sciences, Nepal. Thirty-four patients with clinically diagnosed OSF were randomized into two groups (17 participants in each) with baseline treatment of intralesional dexamethasone with hyaluronidase for 6 weeks for the both. Curcumin (2gm/day) was provided to Group A (Test) and Group B (Control) received placebo. Interincisal mouth opening, tongue protrusion, cheek flexibility and visual analogue scale (VAS) scoring of burning sensation of oral mucosa was recorded at baseline, 6, 8 and 12 weeks follow-up and independent t-test was used to compare the improvements in two groups. Results: On comparing the 6 weeks and baseline values, in Group A and B the mean difference in mouth opening was 8.82±1.33 mm and 5.53±1.17 mm respectively (p<0.001), in cheek flexibility was 2.94±1.02 mm and 1.94±1.24 mm respectively (p=0.02) and in tongue protrusion was 6.23±1.48 and 3.65±1.37 mm respectively (p<0.001). The findings were consistent in the 8 weeks follow-up. In 12 weeks follow-up, on comparing with the baseline values, in Group A and B, the mean difference in mouth opening was 8.71±1.16 mm and 5.35±1.22 mm respectively (<0.001), ), in cheek flexibility was 2.81±1.01 mm and 1.76±1.35 mm respectively (p=0.02) and in tongue protrusion was 6.06±1.48 and 3.35±1.50 mm respectively (p<0.001). Both the arms showed 100% improvement in burning sensation in 6, 8 and 12 weeks follow-up. Conclusion: Curcumin in combination with intralesional dexamethasone with hyaluronidase is efficacious in the treatment of OSF.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
3125
3132
https://journal.waocp.org/article_90275_277e18dd6ab1e3c366e246aa5e7e1db8.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.3125
A Distributed Cancer Care Model with a Technology-Driven Hub-and-Spoke and further Spoke Hierarchy: Findings from a Pilot Implementation Programme in Kerala, India
Venkataramanan
Ramachandran
Institute of Digital Healthcare, WMG, University of Warwick, Coventry, CV4 7AL, United Kingdom.
author
Akash
Pradhan
Karkinos Healthcare, 301 -
3rd Floor, Poonam Chambers, A Wing, Worli, Mumbai, India.
author
Abhishek
Kumar
Karkinos Healthcare, 301 -
3rd Floor, Poonam Chambers, A Wing, Worli, Mumbai, India.
author
Bharat
Sarvepalli
Karkinos Healthcare, 301 -
3rd Floor, Poonam Chambers, A Wing, Worli, Mumbai, India.
author
Sripriya
Rao
Karkinos Healthcare, 301 -
3rd Floor, Poonam Chambers, A Wing, Worli, Mumbai, India.
author
Kunal
Oswal
Karkinos Healthcare, 301 -
3rd Floor, Poonam Chambers, A Wing, Worli, Mumbai, India.
author
Raja
Kommu
Karkinos Healthcare, 301 -
3rd Floor, Poonam Chambers, A Wing, Worli, Mumbai, India.
author
Manish
Sharma
Karkinos Healthcare, 301 -
3rd Floor, Poonam Chambers, A Wing, Worli, Mumbai, India.
author
Sarika
Pathak
Karkinos Healthcare, 301 -
3rd Floor, Poonam Chambers, A Wing, Worli, Mumbai, India.
author
Ramdas
Kunnambath
Karkinos Healthcare, 301 -
3rd Floor, Poonam Chambers, A Wing, Worli, Mumbai, India.
author
Moni Abraham
Kuriakose
Karkinos Healthcare, 301 -
3rd Floor, Poonam Chambers, A Wing, Worli, Mumbai, India.
author
Sankaranarayanan
Rengaswamy
Karkinos Healthcare, 301 -
3rd Floor, Poonam Chambers, A Wing, Worli, Mumbai, India.
author
Mohannad
Alajlani
Institute of Digital Healthcare, WMG, University of Warwick, Coventry, CV4 7AL, United Kingdom.
author
Theodoros N.
Arvanitis
Institute of Digital Healthcare, WMG, University of Warwick, Coventry, CV4 7AL, United Kingdom.
author
text
article
2022
eng
Background: The technology enabled distributed model in Kerala is based on an innovative partnership model between Karkinos Healthcare and private health centers. The model is designed to address the barriers to cancer screening by generating demand and by bringing together the private health centers and service providers at various levels to create a network for continued care. This paper describes the implementation process and presents some preliminary findings. Methods: The model follows the hub-and-spoke and further spoke framework. In the pilot phases, from July 2021 to December 2021, five private health centers (partners) collaborated with Karkinos Healthcare across two districts in Kerala. Screening camps were organized across the districts at the community level where the target groups were administered a risk assessment questionnaire followed by screening tests at the spoke hospitals based on a defined clinical protocol. The screened positive patients were examined further for confirmatory diagnosis at the spoke centers. Patients requiring chemotherapy or minor surgeries were treated at the spokes. For radiation therapy and complex surgeries the patients were referred to the hubs. Results: A total of 2,459 individuals were screened for cancer at the spokes and 299 were screened positive. Capacity was built at the spokes for cancer surgery and chemotherapy. A total of 189 chemotherapy sessions and 17 surgeries were performed at the spokes for cancer patients. 70 patients were referred to the hub. Conclusion: Initial results demonstrate the ability of the technology Distributed Cancer Care Network (DCCN) system to successfully screen and detect cancer and to converge the actions of various private health facilities towards providing a continuum of cancer care. The lessons learnt from this study will be useful for replicating the process in other States.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
3133
3139
https://journal.waocp.org/article_90294_07b35a4a26e6770177fbbb58e2212b82.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.3133
Alterations in p53 Influence hTERT, VEGF and MMPs Expression in Oral Cancer Patients
Ragini
Singh
Department of Biochemistry, All India Institute of Medical Sciences (AIIMS), Rajkot-360110, Gujarat, India.
author
Kinjal
Patel
Molecular Oncology
Laboratory, Cancer Biology Department, The Gujarat Cancer & Research Institute, Asarwa, Ahmedabad -380 016, Gujarat, India.
author
Jayendra
Patel
Molecular Oncology
Laboratory, Cancer Biology Department, The Gujarat Cancer & Research Institute, Asarwa, Ahmedabad -380 016, Gujarat, India.
author
Prabhudas
Patel
Molecular Oncology
Laboratory, Cancer Biology Department, The Gujarat Cancer & Research Institute, Asarwa, Ahmedabad -380 016, Gujarat, India.
author
text
article
2022
eng
Background: Mutant p53 is the crucial molecule in the etiopathogenesis of oral cancer. Therefore, we aimed to evaluate the impact of alterations of the p53 gene and its negative feedback regulator, MDM2, on the expression of hTERT, VEGF, and MMPs; the critical genes involved in oral cancer progression. Material and methods: p53 and MDM2 genotyping were done by PCR-RFLP. p53 mutation analysis was performed using PCR-SSCP and sequencing. hTERT, VEGFA isoforms, MMP2, and MMP9 mRNA levels were analyzed by semi-quantitative Reverse Transcriptase PCR. Results: Arg allele at p53 exon 4 was significantly associated with overexpression of hTERT, MMP2, and MMP9 individually. Expression of hTERT, VEGF A isoforms, MMP2 and MMP9 were significantly altered in the presence of p53 and MDM2 polymorphisms and p53 mutations in a specific combination. Mutant p53, Arg allele at p53 exon 4 locus, and G/G/or T/T genotype at MDM2revealed increased expression of hTERT, VEGF A isoforms, and MMP2/9.Conclusion: This study provides evidence that apart from mutant p53, naturally occurring sequence variants in p53codon 72 (Arg72Pro) (rs1042522) and MDM2 (rs2279744) significantly alter the expression of hTERT, VEGF-A isoforms, and MMP2/9 in a specific combination. The differential interaction of codon 72 variants with MDM2, hTERT, VEGF-A isoforms and MMP2/9 play a role in the aggressiveness of oral cancer. The results have important implications for oral cancer progression and should be explored for innovative treatment options.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
3141
3149
https://journal.waocp.org/article_90276_9146beb3036790beceae5206673d4006.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.3141
The Effect of MicroRNA-409-3p for Treatment and Response to Tumor Proliferation of Lung Cancer Cell Lines (In Vitro)
hany
Al-hussaniy
Department of Pharmacy, Bilad Alrafidain University College, Diyala Junction, Baqubah, Diyala, Iraq.
author
text
article
2022
eng
Objective: Monitoring the result of miR 409 3p and its response to tumor proliferation and its mechanism of action on some types of lung cancer in vitro (A549 cell line). Methods: Two A549 cell line group negative control group with oligonucleotide cultured under conventional conditions and transfected with positive control nucleotides. Experiments Based on the control group, chemically synthesized miR 409 3p mimics were used in the positive group with liposome transfection to construct A549 cells with high miR-409-3p expression. Result: miR4093p expression was estimated using the qPCR method in the two groups after 48 h. Later, the miR-409-3p expression in A549 cells obviously increased significantly with a positive attitude in the positive control group that was transfected by miR-409-3p (mimics) (P<0.20). As a result of this investigation, a significant increase in the percentage of total cell apoptosis was significantly increased in the positive group compared to the control group (22.68% ± 4.62%), (7.79%±1.94%) respectively, (P<0.05). However, in terms of the G1 phase, the rate is obviously low compared to the control group (40.22%±5.36%); (56.08%±5.21%) (P<0.05). endogenous ELF2 was considerably reduced after overexpression of the miR-409-3p mimic (P<0.05). Conclusion: miR-409-3p may prevent non-small cell lung cancer (NSCLC) by affecting ELF2 transcription and other cellular regulators to regulate A549 cell division and induce apoptosis.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
3151
3156
https://journal.waocp.org/article_90277_0bcada47dcca23f4731fc822bc932a8f.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.3151
The Association between Abdominal Obesity, Metabolic Syndrome and Survival Outcomes in Patients with Breast Cancer
Kartika
Taroeno-Hariadi
Division of Hematology and Medical Oncology, Department of Internal Medicine, Faculty of Medicine, Public Health and
Nursing, Universitas Gadjah Mada; Dr. Sardjito Hospital, Yogyakarta, Indonesia.
author
Yasjudan
Putra
Division of Hematology and Medical Oncology, Department of Internal Medicine, Faculty of Medicine, Public Health and
Nursing, Universitas Gadjah Mada; Dr. Sardjito Hospital, Yogyakarta, Indonesia.
author
Nina
Ariesta
Department of Internal Medicine, Faculty
of Medicine, Public Health and Nursing, Universitas Gadjah Mada; Dr. Sardjito Hospital, Yogyakarta, Indonesia.
author
Hemi
Sinorita
Division
of Endocrinology and Metabolic Disease, Department of Internal Medicine, Faculty of Medicine, Public Health and Nursing,
Universitas Gadjah Mada; Dr. Sardjito Hospital, Yogyakarta, Indonesia.
author
Mardiah
Hardianti
Division of Hematology and Medical Oncology, Department of Internal Medicine, Faculty of Medicine, Public Health and
Nursing, Universitas Gadjah Mada; Dr. Sardjito Hospital, Yogyakarta, Indonesia.
author
Irianiwati
Widodo
Department of Pathology, Faculty of Medicine, Public
Health and Nursing, Universitas Gadjah Mada; Dr. Sardjito Hospital, Yogyakarta, Indonesia.
author
Teguh
Aryandono
Div. Oncology Surgery, Department
of Surgery, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada; Dr. Sardjito Hospital, Yogyakarta,
Indonesia.
author
text
article
2022
eng
Background: Obesity and metabolic syndrome (MetS) have been linked to the risk of developing certain cancers. This study aimed to analyze the association between obesity markers, MetS and survival outcomes of patients with breast cancer. Methods: This study retrospectively investigated patients with hormone receptor-positive, human epidermal growth factor receptor 2-negative (HR+/HER2-), nonmetastatic breast cancer diagnosed between January 2010 and December 2019. Data on clinical conditions, body mass index (BMI), waist-to-hip ratio (WHR), MetS, time of metastasis and death were collected. Results: A total of 223 breast cancer patient records were eligible for analysis. Obesity (BMI ≥ 25) was found in 38.1% of cases. Abdominal obesity measured as WHR ≥ 0.85 was found in 48.9%. Metabolic syndrome was detected in 56.1% of patients and was associated with older age (OR = 2.196, p = 0.005), postmenopausal status (OR = 2.585, p = 0.001), obesity (OR = 5.684, p = 0.001) and abdominal obesity (OR = 2.612, p = 0.001). Obesity was not associated with poor disease-free survival (DFS) or overall survival (OS), while abdominal obesity was modestly associated with poor DFS (HR = 1.539, p = 0.083) and OS (HR = 3.117; p = 0.019). Multivariate analysis revealed that WHR ≥ 0.85 was independently associated with unfavorable DFS (HR = 1.907, p = 0.027). Patients with MetS had a similar survival rate to those with normal metabolism. Conclusion: In Indonesian women with HR+/HER2- breast cancers, obesity and MetS were not associated with poor survival outcomes. The abdominal obesity marker (WHR) was more accurate in predicting unfavorable DFS.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
3157
3165
https://journal.waocp.org/article_90278_67e6adab0cca183df99c093677a1e898.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.3157
Psychometric Properties of MD Anderson Symptoms Inventory for Acute Myeloid Leukemia Patients in Thailand
Thanawat
Rattanathammethee
Division of Hematology, Department of Internal Medicine, Faculty of Medicine, Chiang Mai University, Thailand.
author
Omjai
Wongkhut
Department of Internal Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
author
Teerachat
Punnachet
Division of Hematology, Department of Internal Medicine, Faculty of Medicine, Chiang Mai University, Thailand.
author
Nonthakorn
Hantrakun
Division of Hematology, Department of Internal Medicine, Faculty of Medicine, Chiang Mai University, Thailand.
author
Pokpong
Piriyakhuntorn
Division of Hematology, Department of Internal Medicine, Faculty of Medicine, Chiang Mai University, Thailand.
author
Sasinee
Hantrakool
Division of Hematology, Department of Internal Medicine, Faculty of Medicine, Chiang Mai University, Thailand.
author
Chatree
Chai-Adisaksopha
Division of Hematology, Department of Internal Medicine, Faculty of Medicine, Chiang Mai University, Thailand.
author
Ekarat
Rattaritamrong
Division of Hematology, Department of Internal Medicine, Faculty of Medicine, Chiang Mai University, Thailand.
author
Adisak
Tantiworawit
Division of Hematology, Department of Internal Medicine, Faculty of Medicine, Chiang Mai University, Thailand.
author
Lalita
Norasetthada
Division of Hematology, Department of Internal Medicine, Faculty of Medicine, Chiang Mai University, Thailand.
author
text
article
2022
eng
Background: The MD Anderson Symptoms Inventory for acute myeloid leukemia/myelodysplastic syndrome (MDASI-AML/MDS) is a specific patient-reported outcome measure (PROM) and widely used to assess the quality of life of acute myeloid leukemia (AML) patients. This study aimed to validate the inventory in Thai AML patients. Methods: After receiving permission, the original MDASI-AML/MDS was translated and cross-culturally adapted to Thai. Twenty AML patients were included in the study. Internal consistency was evaluated using Cronbach’s alpha and test-retest reliability was analyzed using intraclass correlation coefficient (ICC). Spearman’s rank correlation was used to investigate the subscales of Thai MDASI-AML/MDS and Thai version of European Quality of Life-5 Dimension-5 Level (Thai EQ-5D-5L). Results: All subscales of Thai MDASI-AML/MDS showed an acceptable Cronbach’s alpha (0.64-0.91). The test-retest reliability of each subscale was adequate (ICC = 0.88-0.95). The core symptoms subscale in the Thai MDASI-AML/MDS strongly correlated to the anxiety/ depression subscale in the Thai EQ-5D-5L (r = 0.69, p = 0.0006). A strong correlation was demonstrated between the interference subscale of the Thai MDASI-AML/MDS and the usual activities subscale of Thai EQ-5D-5L (r = 0.77, p = 0.0001). A weak correlation was found between the MDS/AML specific symptoms subscale in the Thai MDASI-AML/MDS and anxiety and depression subscale in the Thai EQ-5D-5L (r = 0.49, p = 0.0285). The Thai MDASI-AML/MDS had strong correlation with Thai EQ-5D-5L (r = 0.71, p = 0.0050). Conclusions: The Thai MDASI-AML/MDS provides adequate internal consistency in all subscales as well as good construct validity and reliability for Thai patients.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
3167
3172
https://journal.waocp.org/article_90296_59b9d8f5fd876b440ef07804bb839067.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.3167
Comparison of Overall Survival between Transarterial Chemoembolization and Best Supportive Care in Intermediate- Stage Hepatocellular Carcinoma
Keerati
Akarapatima
Division of Gastroenterology, Department of Internal Medicine, Hatyai Hospital, Songkhla, Thailand.
author
Arunchai
Chang
Division of Gastroenterology, Department of Internal Medicine, Hatyai Hospital, Songkhla, Thailand.
author
Tanaporn
Prateepchaiboon
Department of Internal Medicine, Hatyai Hospital, Songkhla, Thailand.
author
Nuttanit
Pungpipattrakul
Department of Internal Medicine, Hatyai Hospital, Songkhla, Thailand.
author
Apiradee
Songjamrat
Division of Intervention Radiology, Department of Radiology, Hatyai Hospital, Songkhla, Thailand.
author
Songklod
Pakdeejit
Division of Intervention Radiology, Department of Radiology, Hatyai Hospital, Songkhla, Thailand.
author
Attapon
Rattanasupar
Division of Gastroenterology, Department of Internal Medicine, Hatyai Hospital, Songkhla, Thailand.
author
text
article
2022
eng
Objective: The Thailand management guideline allows the use of transarterial chemoembolization (TACE) for the treatment of intermediate-stage hepatocellular carcinoma (HCC) in patients with decompensated cirrhosis, whereas other guidelines do not. The aim of this study was to compare the overall survival between TACE and the best supportive care (BSC) in HCC patients with Child–Pugh score 5–8 cirrhosis and in subgroups with compensated cirrhosis (Child–Pugh score 5–6) and early decompensated cirrhosis (Child–Pugh score 7–8). Methods: This retrospective study comprised 118 patients with intermediate-stage HCC. The overall survival was compared between TACE and BSC using the Kaplan–Meier method. Results: The median overall survival time for all patients was 21.4 months in the TACE group and 8.2 months in the BSC group (P <0.001). In the subgroup analyses, the overall survival times for TACE and BSC were 26 months and 9 months, respectively, for compensated cirrhosis (P <0.001), and 14.5 months and 6.9 months, respectively, for early decompensated cirrhosis (P <0.001). In the Cox proportional-hazards model, TACE was an independent prognostic factor for prolonged overall survival in all patients [hazard ratio (HR) 0.29; 95% confidence interval (CI), 0.17–0.49; P <0.001], patients with compensated cirrhosis (HR, 0.31; 95% CI, 0.16–0.62; P <0.001), and patients with early decompensated cirrhosis (HR, 0.16; 95% CI, 0.061–0.44; P <0.001). Conclusion: TACE improves the overall survival in patients with intermediate-stage HCC and compensated or early decompensated cirrhosis.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
3173
3178
https://journal.waocp.org/article_90285_244b53dabf4403fc1ab82580f26bcb1b.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.3173
KRAS Mutation in Pediatric Intracranial Germ Cell Tumors
Chusana
Khaiman
Integrative and Innovative Hematology/Oncology Research Unit, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
author
Piti
Techavichit
Integrative and Innovative Hematology/Oncology Research Unit, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
author
Hansamon
Poparn
Integrative and Innovative Hematology/Oncology Research Unit, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
author
Kanhatai
Chiangthong
Integrative and Innovative Hematology/Oncology Research Unit, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
author
Supanun
Lauhasurayotin
Integrative and Innovative Hematology/Oncology Research Unit, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
author
Darintr
Sosothikul
Integrative and Innovative Hematology/Oncology Research Unit, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
author
Kanjana
Shotelersuk
Division of Radiation Oncology, Department of Radiology, King Chulalongkorn Memorial Memorial Hospital, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
author
Chonnipa
Nantavithya
Division of Radiation Oncology, Department of Radiology, King Chulalongkorn Memorial Memorial Hospital, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
author
Ketsuda
Jakchairoongruang
Department of Radiology, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
author
Jiraporn
Amornfa
Division of Neurosurgery, Department of Surgery, King Chulalongkorn Memorial Hospital, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
author
Ekkachai
Khongkhaduead
Chulalongkorn GenePRO Center, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
author
Sakun
Santisukwongchote
Department of Pathology, King Chulalongkorn Memorial Hospital, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
author
Chinachote
Teerapakpinyo
Chulalongkorn GenePRO Center, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
author
Shanop
Shuangshot
Chulalongkorn GenePRO Center, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
author
text
article
2022
eng
Background: Intracranial germ cell tumors (IGCTs) are rare, highly curable neoplasms. KRAS is a gene in the KIT/RAS signaling pathway, and KRAS mutations have been reported in patients diagnosed with IGCTs. Objectives: To describe the clinicopathologic and molecular features of KRAS mutation and the treatment outcome of children diagnosed with IGCTs. Methods: Patients diagnosed with IGCTs at the Department of Pediatrics, King Chulalongkorn Memorial Hospital from 2007 to 2016 were retrospectively reviewed. DNA was extracted from formalin-fixed, paraffin-embedded tissue and used for molecular study. Mutations in codons 12, 13, and 61 of the KRAS gene were detected using the cobas® KRAS mutation test and pyrosequencing. Results: Eighteen patients were diagnosed with IGCTs (11 males and 7 females): nine with germinomas and nine with non-germinomatous GCTs (NGGCTs). The age range of the patients was 5–14 years (median 10.5 years). Elevated markers were revealed in approximately 25% of the patients. Four patients (two with germinomas and two with NGGCTs) had leptomeningeal involvement. All patients underwent tumor biopsy and received neoadjuvant chemotherapy. Radiotherapy was administered in 16 patients, and craniospinal radiation was administered only in patients with leptomeningeal metastasis. With a median follow-up of 26 months, overall survival was 88.9% in the patients with germinomas and 37% in the patients with NGGCTs. Mutation of the KRAS gene was detected using pyrosequencing in one patient. The mutation located at codon 61, with frequency 38.3% units, nucleotide substitution CAA > CTA, and amino acid substitution, was Q61L. The patient carrying the mutant gene was diagnosed with germinoma with cerebrospinal fluid metastasis and eventually died from treatment-related toxicity. Conclusion: Our study revealed the treatment outcomes of IGCTs in Thai children. The metastatic germinoma patient with KRAS codon 61 mutation had a poor outcome, supporting that Q61L has a clinical correlation with IGCTs.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
3179
3185
https://journal.waocp.org/article_90286_239732b4d2d2b233953131e60c69b76c.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.3179
Evaluation of In Vitro Cytotoxic Property Against Cholangiocarcinoma Cell Line and GC/MS Analysis from Leaf of Erythrophleum succirubrum Gagnep
Sukanda
Chaiyong
Department of Biology, Faculty of Science, Chandrakasem Rajabhat University, Bangkok 10900, Thailand.
author
Nataya
Sutthanont
Department of
Medical Entomology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand.
author
Apaporn
Menakongka
Department of Basic Medical
Science, Faculty of Medicine Vajira Hospital, Navamindradhiraj University, Bangkok 10300, Thailand.
author
text
article
2022
eng
Objective: Plants are valuable sources of new pharmaceuticals. Secondary metabolites of the genus Erythrophleum exhibit cytotoxicity and may have therapeutic value. The cytotoxic activity of ethanolic leaf extract of Erythrophleum succirubrum Gagnep. against a human cholangiocarcinoma cell line was assessed. Methods: Crude extract of E. succirubrum was prepared by ethanol extraction. The ethanolic leaf extract of E. succirubrum was evaluated for cytotoxicity against the human cholangiocarcinoma cell line KKU-M213 using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assays. The chemical composition of E. succirubrum leaf extract was analyzed using GC/MS. Result: The ethanolic leaf extract of E. succirubrum reduced the viability of KKU-M213 cells in a dose- and time-dependent manner. It showed high cytotoxicity, with IC50 values of 65.22 ± 1.18 µg/mL and 1.19 ± 1.38 µg/mL at exposure times of 24 and 96 h, respectively. GC/MS analysis of the ethanolic leaf extract of E. succirubrum identified 22 components. The main constituents identified were Cyclohexanone, 2-[2-nitro-1-(2-naphthyl)ethyl]-(14.79%) followed by allomycin (14.65%), mome inositol (14.30%), campesterol (11.80%) and ethyl linolenate (10.83%), respectively. Conclusion: Five major groups of compounds were found, with lipids dominating, followed by carbohydrates, benzenoids, phenylpropanoids, polyketides and organoheterocyclic compounds. Many of the bioactive components discovered in the ethanolic leaf extract of E. succirubrum might be responsible for its cytotoxic properties.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
3187
3194
https://journal.waocp.org/article_90298_18047dff6bd93a841948c2ce2cd7077e.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.3187
The Effect of Intragastric Gavage of High Dose Green Tea Extract on Serum Status of Magnesium, Calcium, and Zinc
Ameera
Khaleel
School of Dental Sciences, Health Campus, Universiti Sains Malaysia, Kubang Kerian, 16150 Kota Bharu, Kelantan, Malaysia.
author
Ramizu
Shaari
School of Dental Sciences, Health Campus, Universiti Sains Malaysia, Kubang Kerian, 16150 Kota Bharu, Kelantan, Malaysia.
author
Mohamad Arif
Nawi
School of Dental Sciences, Health Campus, Universiti Sains Malaysia, Kubang Kerian, 16150 Kota Bharu, Kelantan, Malaysia.
author
Ali Mihsen
Al-Yassiri
Head of Dentistry Department, Hilla University College, Babylon, Iraq.
author
text
article
2022
eng
Objective: Green tea (GT) contains polyphenolic flavonoids, different minerals like magnesium, calcium, and zinc, vitamins, amino acids, carbohydrates, proteins, and others. It has a different health benefit. The aim of the present study was to investigate the effect of intragastric gavage of a high dose GT extract on serum biochemical analysis of magnesium, calcium, and zincin juvenile Wistar albino rats. Methods: Twelve rats were used in the study and divided in to two equal groups. All the animals in the control group were intragastically gavaged by distilled water and continues for ten days, from day 24 to day 34 of age, while the animals in the study group were intragastically gavaged by GT extract (300mg/kg/day) which continues also for ten days from day 24 to day 34 of age. On day 34 of age, and two hours after the last dose, the rats were anaesthetized and blood collection by cardiac puncture was taken. Results: The results showed that the intragastric gavage of a high dose of GT extract caused a non-significant increase in serum magnesium, and calcium levels (p>0.05), but a significant increase in zinc serum level was seen(p< 0.05). Conclusion: GT can cause a significant increase in zinc serum level, and this may explain the significant role of GT in the response to different oxidative stress. It is recommended to measure the Zn serum level in rats after a period longer than two hrs from the time of the last dose of intragastric gavage of GT extract.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
3195
3199
https://journal.waocp.org/article_90287_da49c69a13662d21e632b5b64842c0c9.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.3195
Detection of High-Risk Human Papillomavirus DNA in Invasive Ductal Carcinoma Specimens
Mastaneh
Alinezhadi
Cancer Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
author
Manoochehr
Makvandi
Department of Virology, School of
Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
author
Gholam
Kaydani
Department of Laboratory Sciences, School of Allied
Health Sciences Ahvaz Jundishapur University of Medical sciences Ahvaz, Iran.
author
Seyed
Jazayeri
Department of Pathology, Imam Khomeini
Hospital, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
author
Javad
Charostad
Department of Virology, School of
Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
author
Abdolhassan Talaiezadeh
Talaeizadeh
Cancer Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
author
Kambiz
Angali
Department of Biostatistics and Epidemiology, Faculty
of Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
author
text
article
2022
eng
Background: According to several studies, there is an association between human papillomavirus (HPV) and breast cancer. Therefore, detection and genotyping of HPV seem important. The present study aimed to investigate the presence of HPV DNA in breast tissues by analyzing the L1 gene. Materials and Methods: This case-control study was conducted on 63 formalin-fixed paraffin-embedded (FFPE) tissues of invasive ductal carcinoma (IDC) as the case group and 32 FFPE tissues of fibroadenoma as the control group. HPV DNA was detected using the polymerase chain reaction assay. Positive samples were then subjected to genotyping. All statistical analyses were performed in SPSS version 22.0. Results: The patients’ age ranged from 15 to 92 years, with a mean age of 43.54±16.36 years. HPV DNA was detected in 17/95 (17.89%) samples, including 9/32 (28.12%) fibroadenoma samples and 8/63 (12.69%) IDC samples. No significant difference was observed regarding the presence of HPV DNA between the IDC and fibroadenoma tissues (P=0.08). However, a significant difference was found in the detection of high-risk HPV (HR-HPV) between the case and control groups (P=0.03). In the case group, 87.5% of the detected viruses (7/8 samples) were HR-HPV, while in the control group, 22.22% of positive samples (2/9 samples) were HR-HPV (P=0.03). Based on the results, HR-HPV and low-risk HPV genotypes were detected in 53% (9/17) and 47% (8/17) of positive samples, respectively. Conclusion: In this study, 12.69% of IDC samples were positive for HPV genomes, and HR-HPV was detected in 87.5% of these samples. The present results suggest the important role of HR-HPV in the development of breast cancer.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
3201
3207
https://journal.waocp.org/article_90303_9986ffe525fee737671eaa9544dcbb7b.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.3201
Could Amifostine Prevent Experimental Radiotherapy-Induced Acute Pericarditis?
Zumrut
Bahat
Karadeniz Technical University, Faculty of Medicine, Department of Radiation Oncology, 61080, Trabzon, Turkey.
author
Umit
Cobanoglu
Karadeniz
Technical University, Faculty of Medicine, Department of Pathology, 61080, Trabzon, Turkey.
author
Cunay
Ulku
Karadeniz Technical University,
Faculty of Medicine, Department of Pharmacology, 61080, Trabzon, Turkey.
author
Nuri
Kalyoncu
Karadeniz Technical University,
Faculty of Medicine, Department of Pharmacology, 61080, Trabzon, Turkey.
author
Suleyman
Caner Karahan
Karadeniz Technical University, Faculty of Medicine,
Department of Biochemistry, 61080, Trabzon, Turkey.
author
Melek
Yavuz
Karadeniz Technical University, Faculty of Medicine, Department of Radiation Oncology, 61080, Trabzon, Turkey.
author
text
article
2022
eng
Background: Amifostine is a powerful antioxidant that is one of the documented three chemo-radio prototectants recommended for clinical use. There is no data exploring amifostine in prevention of acute pericardial damage. We aimed to investigate whether amifostine has protective effect against acute pericardial injury due to radiotherapy in an experimental rat model. Methods: Twenty-four rats were divided into four groups: control group, radiotherapy-only group, amifostine-only group, radiotherapy+amifostine group. In groups receiving radiotherapy, hearts were irradiated with a Co 60 teletherapy device at a distance of 80 cm and 20 Gy at a depth of 2 cm. Thirty minutes before interventions, 200 mg/kg amifostine or same volume 0.9% NaCl were administered intraperitoneally. Subjects were sacrificed 24 hours after the procedure. Pericardial histopathological changes were investigated by light microscopy. Results: There was focal inflammation of >= 50% in all rats exposed-to-radiotherapy. All groups receiving radiotherapy revealed a significant increase in pericardial inflammation compared to the groups that did not receive irradiation (p<0.05). There was no difference between the radiotherapy-only group and amifostine+radiotherapy group for pericardial inflammatory response (p>0.05). Conclusion: Acute pericarditis was detected in all rats receiving radiotherapy. There was no positive effect of amifostine administration before radiotherapy on acute pericardial inflammation.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
3209
3213
https://journal.waocp.org/article_90304_7d5e963d2b437210b36a76b399b3a4ae.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.3209
Blockage of Wnt/β-catenin Signaling Pathway in Colorectal Cancer Resistant Cells by Nitazoxanide Effects on Peptidylarginine Deiminases Expression
Mohsen
Hemmati-Dinarvand
Department of Clinical Biochemistry, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran.
author
Shahnaz
Kheirandish
Department of Medical Genetics, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
author
Ali
Khodadadian
Department of Medical Genetics, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
author
Mostafa
Mostafazadeh
Department of Clinical Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
author
Atefeh
Seghatoleslam
Department of Clinical Biochemistry,
Faculty of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
author
text
article
2022
eng
Background: Multidrug resistance (MDR) is a major cause of unsuccessful cancer treatment in which drugs are not effective. Therefore, it is necessary to identify the critical mechanisms of the development of MDR and target those with novel compounds. Accordingly, the current study is the first to investigate the combination effect and molecular mechanism of nitazoxanide (NTZ) and oxaliplatin (OXP) on LS174T/OXP-resistant cells. Methods: The effect of NTZ on OXP cytotoxicity in LS174T and LS174T/OXP cell lines was evaluated by MTT assay. Changes in expression levels of MDR1, MRP1, CTNNB1, peptidylarginine deiminase (PAD)2, and PAD4 genes and proteins were evaluated by RT-qPCR and western blotting methods, respectively. Lastly, the apoptosis assay was performed by flow cytometer. Results: OXP resistant and sensitive cells were identified based on the IC50 values (11567 nM vs. 1745 nM, p<0.05 for 24 h treatment; and 5161 nM vs. 882 nM, p<0.05 for 48 h incubation). The combination of NTZ and OXP for 48 h led to a reduction in IC50 values in resistant cells (2154 nM, p<0.05). The effect of NTZ plus OXP significantly decreased the expression of MDR1 (p<0.001), MRP1 (p<0.05), and CTNNB1 (p<0.001), while PAD2 and PAD4 expression was significantly increased (p<0.001). This combination therapy enhanced the percentage of the sub-G1 population (apoptosed) compared to other groups. Conclusion: The results showed that NTZ leads to notable upregulation of PAD2 and PAD4, which can disrupt the Wnt/β-catenin signaling pathway and reverse the MDR by reducing MDR1 and MRP1 expression.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
3215
3222
https://journal.waocp.org/article_90299_9b8bb0a92282df474e07110e2c1803ea.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.3215
Factors Associated with Dysplastic Changes in Sinonasal Inverted Papilloma: Study of Tumor Infiltrating Lymphocytes (TILs) FOXP3, CD4, CD8, and expression of p53
Lisnawati
Rachmadi
Department of Pathology, Faculty of Medicine Universitas Indonesia, Cipto Mangunkusumo Hospital Jakarta, Indonesia.
author
Yayi
Billianti Susanto
Department of Pathology, Faculty of Medicine Universitas Indonesia, Cipto Mangunkusumo Hospital Jakarta, Indonesia.
author
Amelia
Manatar
Department of Pathology, Faculty of Medicine Universitas Indonesia, Cipto Mangunkusumo Hospital Jakarta, Indonesia.
author
David
Sitinjak
Department of Pathology, Faculty of Medicine Universitas Indonesia, Cipto Mangunkusumo Hospital Jakarta, Indonesia.
author
text
article
2022
eng
Objective: This study examine FOXP3, CD4, CD8 and p53 expression in the transformation of the Sinonasal Inverted Papilloma (SIP) malignancy into sinonasal carcinoma. Materials and Methods: This study used a cross-sectional approach. The research sample from thirty-six paraffin block preparations with the diagnosis of SIP. Then, immunohistochemical staining was performed using FOXP3 mouse monoclonal antibody (236A/E7), CD8 rabbit monoclonal antibody (CD8/1179R), CD4 mouse monoclonal antibody (4B12) and p53 rabbit monoclonal antibody. Results: There was a significant difference between Foxp3 expression in SIP without dysplasia and SIP with dysplasia (p= 0.013). There was no significant difference between the expression of CD4 and CD8 in the two groups with p-values 0.1 and 0.062, respectively. The mean percentage of positive p53 expression in SIP without dysplasia was 0.45+0.63 and in the SIP with dysplasia 29.31+38.96. There was a significant difference between the two groups (p<0.001). Conclusion: FOXP3 and p53 were overexpressed in SIP with malignant transformation. FOXP3 together with p53 status is associated with dysplastic changed in the SIP. FOXP3 and p53 status could be potential biomarker of malignant transformation in sinonasal inverted papilloma.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
3223
3227
https://journal.waocp.org/article_90302_1a1c8cacad7575ed0202854de519d100.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.3223
Interleukin-10-1082A>G (rs1800896) Single Nucleotide Polymorphism is Not a Risk Factor of Chronic Lymphocytic Leukemia in Sudanese Population
Ameen
Basabaeen
Department of Hematology, Faculty of Medical Laboratory Sciences, Al Neelain University, Khartoum, Sudan.
author
Enaam
Abdelgader
Department of Pathology, Faculty of Medicine, Al Neelain University, Khartoum,
Sudan.
author
Ebtihal
Ahmed
Department of Hematology, Faculty of Medical Laboratory Sciences, Al Neelain University, Khartoum, Sudan.
author
Nour
Abdelateif
Department of Hematology, Faculty of Medical Laboratory Sciences, Al Neelain University, Khartoum, Sudan.
author
Sadia
Osman Abdelrahim
Department of Hematology, Faculty of Medical Laboratory Sciences, Al Neelain University, Khartoum, Sudan.
author
Omnia
Sharif
Department of Haematology, Faculty of Medical Laboratory Sciences, Sudan University of Science and Technology,
Khartoum, Sudan.
author
Osama
Altayeb
Flow Cytometry Laboratory for Leukemia & Lymphoma Diagnosis, Khartoum, Sudan.
author
Eman
Fadul
Flow Cytometry Laboratory for Leukemia & Lymphoma Diagnosis, Khartoum, Sudan.
author
Ibrahim
Osman Ibrahim
Department of Hematology, Faculty of Medical Laboratory Sciences, Al Neelain University, Khartoum, Sudan.
author
text
article
2022
eng
Objective: The present study was conducted to examine the association between the IL-10-1082A>G (rs 1800896) polymorphism and risk of Chronic Lymphocytic Leukemia and to assess the correlation between this polymorphism and clinicopathological characters. Methods: A case-control study was conducted in Khartoum state, Sudan, during the period from April 2017 to April 2018, involved 110 CLL patients and 80 healthy volunteers as a control group. Physical examination, complete blood count, and immunophenotype were performed in all patients to confirm the diagnosis. Clinical staging such as Rai and Binet were studied. CD38 and ZAP70 were performed by flow cytometry. Blood samples were collected from all participants; DNA was extracted by using ANALYTIKJENA Blood DNA Extraction Kit and analyzed IL-10-1082A>G polymorphism by using Allele Specific-Polymerase Chain Reaction. The statistical analysis was performed using statistical package for social sciences version 23.0 software. Results: Frequency of AA, AG, and GG genotypes was 32.7%, 55.5%, and 11.8% for the patient group and 31.25%, 51.25%, and 17.5% in the control group, respectively. The genotype of IL-10 (-1082A>G) did not associate with susceptibility of CLL in our population. The study showed that the G allele of the IL-10 gene (-1082A>G) is associated with the male sex. However, no significant association was found between -1082A>G genotype and clinicopathological characters. Conclusion: Our results do not support the involvement of the IL-10 −1082A>G promoter gene polymorphism in the increased CLL susceptibility. IL-10-1082G allele (IL-10-1082AG or IL-10-1082GG) was found more frequently in males. Furthermore, no association was observed between the IL-10-1082A>G polymorphism and clinical stages systems as well as established poor prognostic markers. Finally, within the group of patients with CLL, there was no difference in the age at diagnosis and hematological parameters according to genotype distributions.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
9
no.
2022
3229
3235
https://journal.waocp.org/article_90300_2a7b760b34ddff7df92f5f8cd67b6afe.pdf
dx.doi.org/10.31557/APJCP.2022.23.9.3229