Association of Handgrip Strength with Quality of Life in Breast Cancer Survivors: A Systematic Review and Meta-Analysis
Alana Cristina
Campos e Silva
Federal University of Pernambuco. Graduate program of Physical Therapy. Recife-PE, Brazil.
author
Anke
Bergmann
Clinical Epidemiology Program, Brazilian National Cancer Institute (INCA), Rio de Janeiro, Brazil.
author
Camilla
Araujo
Federal University of Pernambuco. Graduate program of Physical Therapy. Recife-PE, Brazil.
author
Andrea Karla
Montenegro
Federal University of Pernambuco. Graduate program of Physical Therapy. Recife-PE, Brazil.
author
Angelica da Silva
Tenorio
Federal University of Pernambuco. Graduate program of Physical Therapy. Recife-PE, Brazil.
author
Diego
Dantas
Federal University of Pernambuco. Graduate program of Physical Therapy. Recife-PE, Brazil.
author
text
article
2022
eng
Objective: Handgrip strength (HGS) is an indicator of general muscular strength and in cancer patients acts as a relevant marker associated with mortality and health. This study aimed to evaluate the association between peripheral muscle function and health-related quality of life (HRQoL) in breast cancer (BC) survivors. Methods: Systematic review registered on PROSPERO under number: CRD 42021225206. The searches were carried out on MEDLINE via Pubmed, PEDro, Cochrane Library, Embase, CINAHL via EBSCO and Science Direct databases. Observational studies evaluating the association between handgrip strength (HGS) and HRQoL in adult female BC survivors were included. No linguistic or time restrictions were applied. Two reviewers reviewed full texts for inclusion and performed data extraction and risk of bias using the Newcastle and Ottawa scale (NOS). Results: Five articles were included and involved 587 patients, mean age of 47 to 59 years. The percentage of decreased HGS ranged from 38.3% to 60.3%. HGS was associated with different quality of life measures. From meta-analysis including 220 patients, the correlation coefficient between HGS and HRQoL was 0.26 (95% CI: 0.07-0.35). Conclusions: Breast cancer survivors face decline of HGS. In this population HGS was correlated with HRQoL. However, more evidence are necessary.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3237
3245
https://journal.waocp.org/article_90318_ae4f07e683ae4692bcb05b1e31d5422d.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3237
Assessment of Cell Cycle and Induction of Apoptosis by Shiga-like Toxin Produced by Escherichia coli O157:H7 in T47D Breast Cancer Cells Using Flow Cytometry
I Wayan
Suardana
Department of Preventive Veterinary Medicine, Faculty of Veterinary Medicine, Udayana University. Jl. P.B. Sudirman, Denpasar-Bali. 80234. Indonesia.
author
Ida Bagus Ngurah
Swacita
Department of Preventive Veterinary Medicine, Faculty of Veterinary Medicine, Udayana University. Jl. P.B. Sudirman, Denpasar-Bali. 80234. Indonesia.
author
Komang Januartha Putra
Pinatih
Department of Clinical Microbiology, Faculty of Medicine, Udayana University. Jl. P.B.Sudirman, Denpasar-Bali. 80234. Indonesia.
author
Hamong
Suharsono
Department of Basic Veterinary Medicine, Faculty of Veterinary Medicine, Udayana University. Jl. PB. Sudirman, Denpasar-Bali. 80234. Indonesia.
author
Dyah Ayu
Widiasih
Department of Veterinary Public Health, Faculty of Veterinary Medicine, Gadjah Mada University. Jl. Fauna No. 2 Karangmalang, Yogyakarta 55281 Indonesia.
author
text
article
2022
eng
Background: The low general toxicity against tumors expressing globotriaosylceramide (Gb3) and Shiga-like toxins produced by E. coli have been proposed as an anti-cancer therapy because of their specific target. This study aimed to determine the potency of the local strains of E. coli O157:H7 isolated from humans and cattle as a new breast cancer therapy by analyzing the cell cycle’s inhibition and apoptosis induction. Material and Methods: Approximately 10 cultured T47D cells were subjected to Shiga-like toxin produced by four local isolates of E. coli O157:H7, including KL-48 (2) from humans, and SM-25 (1), SM-7 (1), DS-21 (4) from cattle. Using ATCC 43894 as a control, the treatment was observed for 24 h by two replications. In addition, a FITC-Annexin V and PI assay were used to observe apoptosis and necrosis effect, as well as to analyze the cell cycle using propidium iodide (PI) staining. Results: The results showed the toxicity effect of Shiga in the human T47 D cells line. The viability of the cells is subjected to Shiga-like toxins produced by KL-48 (2), SM7 (1), ATCC 43894, SM-25 (1), and DS-21 (4) isolates decreased with 15.20, 16.36, 22.17, 22.64, and 33.86%, in contrary to control of 94.36%. These were supported by the cells entering the late apoptosis of the cell cycle through each isolate with 67.66, 62.60, 63.68, 63.90, and 54.74%, and a control of 0.01%. Also, the necrosis cell for each treatment of 12.73, 19.3, 10.84, 10.53, and 4.86% was higher than the control of 5.51%. These were confirmed by the higher percentage of the cells treated with toxins of KL-48 (2), SM7(1), ATCC 43894, SM-25 (1), and DS-21 (4), which entered G0-G1 of the cell cycle phase with 66.41, 63.37, 61.52, 55.36, and 47.28%, respectively, than control of 40.69%. Additionally, the toxicity effect was supported by an increase in the cells entering the S and the G2-M phase of the cycle for each treatment. Conclusion: It is concluded that the Shiga-like toxin produced by E. coli O157:H7 local isolates can be developed as a drug against breast cancer based on its effect to arrest induction of the cell cycle and inducing apoptosis.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3247
3252
https://journal.waocp.org/article_90313_d317edb5134e9085177b25f137685948.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3247
Evaluation of Cytochrome P4502E1 mRNA Expression and Its Effects in Antioxidant Defenses, and Cell Toxicity in Printing Workers
Brenda
Rodríguez-Romero
National School of Medicine and Homeopathy, National Polytechnic Institute, Mexico City, Mexico.
author
Maritere
Dominguez-Rojas
Faculty of Higher Studies Cuautitlán, National Autonomous University of Mexico, Izcalli, State of Mexico, Mexico.
author
Maria
Medel-Flores
National School of Medicine and Homeopathy, National Polytechnic Institute, Mexico City, Mexico.
author
Nadia
Pérez-Vielma
Interdisciplinary Center for Health Sciences Santo Tomas Unit, National Polytechnic Institute, Mexico City, Mexico.
author
Mario
Mendoza-Garrido
National School of Medicine and Homeopathy, National Polytechnic Institute, Mexico City, Mexico.
author
Virginia
Sánchez-Monroy
Superior School of Medicine, National Polytechnic Institute, Mexico City, Mexico.
author
text
article
2022
eng
Background: Cytochrome P4502E1 (CYP2E1) metabolizes environmental toxins, however, compound metabolism can produce oxidative stress, causing in-cell toxicity and sometimes transformation. Aim: To evaluate CYP2E1 gene expression and its effects in antioxidant defenses, and cell toxicity in printing workers. Methods: The hierarchical method of health and chemical risk was used to evaluate chemical exposure in workplace. Blood samples and buccal epithelial cells were obtained from printing workers, and workers without any history of occupational exposure to chemicals (control group). Gene expression of CYP2E1, and antioxidant enzymes Superoxide dismutase (SOD) and Catalase (CAT) from leukocytes were evaluated. Hematic analysis and cell-free DNA from plasma were analyzed. Frequencies of cells with micronuclei (MN) and nuclear abnormalities from buccal epithelial cells were explored. Results: Evaluation of chemical exposure in working place demonstrated that ethyl alcohol, isopropyl alcohol, and isophorone represent 91% of the accumulated potential risk. CYP2E1 expression showed a 2.5-fold overexpression in the printing workers compared to the control group. SOD expression showed a 0.5-fold lower level in the printing workers than the control group, and CAT expression showed no differences between groups. Lower red blood cell and platelet values were detected in the printing workers than in the control group, and cell-free DNA plasma concentration was 3-fold higher in the printing workers than in the control group. The printing workers showed a higher frequency of cells with MN and nuclear anomalies than the control group. Conclusion: CYP2E1 overexpression triggers antioxidant defenses and toxic cell effects in printing workers.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3253
3259
https://journal.waocp.org/article_90312_f03b906d4ea7e807559017e68f59f5e3.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3253
Validation of Immune Status Questionnaire (ISQ) in Indonesian Bahasa Language as simple assessment of perceived immune status.
Yohana
Azhar
Division of Oncology Surgery, Department of Surgery, Faculty of Medicine, Universitas Padjajaran / Dr. Hasan Sadikin General Hospital, Bandung, Indonesia.
author
Zuldi
Erdiansyah
Department of Surgery, Faculty of Medicine, Universitas Padjadjaran / Dr. Hasan Sadikin General Hospital, Bandung, Indonesia.
author
Reno
Rudiman
Department of Surgery, Faculty of Medicine, Universitas Padjadjaran / Dr. Hasan Sadikin General Hospital, Bandung, Indonesia.
author
text
article
2022
eng
Objective: This study aimed to determine whether the ISQ can be used to assess immune status in daily clinical practice in Indonesia by translating the questionnaire into Bahasa and analyzing its validity and reliability. Methods: The ISQ was translated both forward and backward by a professional translator fluent in both English and Bahasa. In this study, a cross-sectional study design was used, and Pearson’s correlation test and Cronbach’s Alpha calculation were used to perform the validity and reliability test. The questionnaire is valid if the computed R-value exceeds the R table value. Furthermore, it will also be considered reliable if Cronbach’s Alpha value is greater than 0.60. Result: The computed R-value for each item is greater than the R table value (0.361), implying that all items in the questionnaire are valid. The Cronbach’s Alpha value of 0.706 demonstrates that the questionnaire is reliable. Conclusion: The ISQ is a reliable and valid questionnaire, which can be used in Bahasa to assess patients’ perspectives about their immune status.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3261
3263
https://journal.waocp.org/article_90311_ab4a9fd529008b9bee7aa5d9d197e32c.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3261
Measuring Occupational Fatigue among Higher and Middle Management at a Specialized Cancer Center during the COVID-19 Pandemic
Majeda
Al-ruzzieh
Nursing Department, King Hussein Cancer Center, Amman, Jordan.
author
Omar
Ayaad
Quality and Accreditation Department, Sultan Qaboos
Comprehensive Cancer Care and Research Center, Muscat, Oman.
author
text
article
2022
eng
Purpose: This study aims to measure the occupational fatigue level and describe the sources and consequences of occupational fatigue among middle and higher management at an international specialized cancer center during the COVID-19 pandemic. Methods: A mixed-method design was used in this study. A convenience sampling technique was utilized to select the participants from King Hussein Cancer Center, Amman, Jordan. The data was collected through a self-administered questionnaire. This questionnaire included both quantitative and qualitative sections. We utilized Fatigue Risk Assessment and Management in High-Risk Environments (FRAME)-26 items scale for the quantitative section. Two main questions were included in the survey to identify the sources and consequences of occupational fatigue.Results: The results show that the average mean of occupational fatigue was 2.95/5 (SD=0.70). The level of changes in fatigue levels after the COVID-19 pandemic increased by 0.87/3 (SD=1.45). The sources of occupational fatigue could be categorized into five themes: workload, work environment, staffing, psychological, and physical sources. There are four themes categorizing occupational fatigue: social, economic, health, and daily activity and lifestyle. Conclusion: This study affords valuable insight into the level, source, and consequences of occupational fatigue among middle and higher management at an international specialized cancer hospital in developing countries. The results indicate that the occupational fatigue level was moderate, and the fatigue level was negatively impacted by the COVID-19 pandemic.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3265
3271
https://journal.waocp.org/article_90341_21486d5174183a1498062f8d1603624f.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3265
Estimating the Cancer Treatment Cost for 5 Common Types of Cancer with Separating Out-of-Pocket and Governmental Costs in Afghanistan, 2020
Hasan
Rohani
Economic Research center, Kateb University, Kabul, Afghanistan.
author
Sayed Hamid
Mousavi
Medical Research Center, Kateb University, Kabul, Afghanistan.
author
Sayed Mahdi
Hashemy
Medical Research Center, Kateb University, Kabul, Afghanistan.
author
Shakardokht
Jafari
Medical Governance Research Institute, Tokyo, Japan.
author
Ghulam Yahya
Amiry
Medical Research Center, Kateb University, Kabul, Afghanistan.
author
Divya
Bhandari
Medical Governance Research Institute, Tokyo, Japan.
author
Akihiko
Ozaki
Department of Breast Surgery, Jyoban Hospital of Tokiwa Foundation, Fukushima, Japan.
author
Tayeba
Hashemy
Economic Research center, Kateb University, Kabul, Afghanistan.
author
text
article
2022
eng
The objective this study was to estimate the cost of cancer treatment services for 5 common types of cancer (Breast, Esophageal, Colorectal, Stomach and lung) by the public sector and patients. This study was a cross-sectional study that conducted using the medical records of patients who were registered in the Jamhuriyat Hospital from 1, 2020 to 12, 2020. The prevalence-based approach was used to estimate the costs of five major cancer types. The data of 769 patients were eligible to include for the analysis. The considered cost of services was obtained from the average cost in 3 private hospitals in Kabul. Also all costs converted in to US dollar using the exchange rate of each USD equaling to AFN 77 in 2020. The data was analyzed using Ms. Excel Program. The total number of patients with 5 types of cancer were 769. Of these, female, in the age group of 40-60 years and were illiterate. The most common cancers based on the cancer treatment services’ costs of health services was breast cancer, followed by esophagus, colorectal, stomach and lung. Moreover, the most common cancers based on the cancer treatment services’ costs of health services which cancer patients needed was breast cancer, followed by esophagus, colorectal, stomach and lung. Moreover, total cost of cancer treatment is US$590,662.98, in which Patients paid USD 82,537 of it out-of-pocket. The costs of cancer treatment services for 5 common types of cancer are high which a considerable portion are paid by patients. There is a need for the government to pay more attention to the provision of these services and to increase the centers providing these services and the existing facilities. Also, support of the international community in this process can enable more people to access these services and reduce the economic burden on patients.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3273
3279
https://journal.waocp.org/article_90340_29422873fee27407bc6ad8b5adc05277.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3273
Effect of Replacement of Wharton Acellular Jelly With FBS on the Expression of Megakaryocyte Linear Markers in Hematopoietic Stem Cells CD34+
zahra
Jalili
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
author
Behnam
Emamgolizadeh
Student research committee, Tabriz University of
Medical Sciences, Tabriz, Iran.
author
Hossein
Abbaszadeh
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
author
Shahla
Jalili
Department of Applied Cell
Sciences, School of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
author
Mehdi
Derakhshani
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
author
Mehdi
Yousefi
Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz,
Iran.
author
Mehdi
Talebi
Hematology and Oncology
Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
author
Karim
Shams Asenjan
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
author
Ali Akabr
Movassaghpour
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
author
text
article
2022
eng
Objective: Animal environments for the growth of stem cells cause the transmission of some diseases and immune problems for the recipient. Accordingly, replacing these environments with healthy environments, at least with human resources, is essential. One of the media that can be used as an alternative to animal serums is Wharton acellular jelly (AWJ). Therefore, in this study, we intend to replace FBS with Wharton jelly and investigate its effect on the expression of megakaryocyte-related genes and markers in stem cells. Materials and Methods: In this study, cord blood-derived CD34 positive HSCs were cultured and expanded in the presence of cytokines including SCF, TPO, and FLT3-L. Then, the culture of expanded CD34 positive HSCs was performed in two groups: 1) IMDM culture medium containing 10% FBS and 100 ng / ml thrombopoietin cytokine 2) IMDM culture medium containing 10% AWJ, 100 ng / ml thrombopoietin cytokine. Finally, CD41 expressing cells were analyzed with the flow cytometry method. The genes related to megakaryocyte lineage including FLI1 and GATA2 were also evaluated using the RT-PCR technique. Results: The expression of CD41, a specific marker of megakaryocyte lineage in culture medium containing Wharton acellular jelly was increased compared to the FBS group. Additionally, the expression of GATA2 and FLI1 genes was significantly increased related to the control group. Conclusion: This study provided evidence of differentiation of CD34 positive hematopoietic stem cells from umbilical cord blood to megakaryocytes in a culture medium containing AWJ.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3281
3286
https://journal.waocp.org/article_90336_a5809e0471cb0cb2ea542634dd5f090c.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3281
Comparison of Classification Success Rates of Different Machine Learning Algorithms in the Diagnosis of Breast Cancer
Irem
Ozcan
Department of Computer Engineering, Faculty of Engineering and Architecture, Istanbul Gelisim University, Istanbul, Turkey.
author
Hakan
Aydin
Department of Computer Engineering, Faculty of Engineering, Istanbul Topkapı University, Istanbul, Turkey.
author
Ali
Cetinkaya
Department of Electronics Technology, Istanbul Gelisim Vocational School, Istanbul Gelisim University, Istanbul, Turkey.
author
text
article
2022
eng
Objective: To identify which Machine Learning (ML) algorithms are the most successful in predicting and diagnosing breast cancer according to accuracy rates. Methods: The “College of Wisconsin Breast Cancer Dataset”, which consists of 569 data and 30 features, was classified using Support Vector Machine (SVM), Naive Bayes (NB), Random Forest (RF), Decision Tree (DT), K-Nearest Neighbor (KNN), Logistic Regression (LR), Multilayer Perceptron (MLP), Linear Discriminant Analysis (LDA), XgBoost (XGB), Ada-Boost (ABC) and Gradient Boosting (GBC) ML algorithms. Before the classification process, the dataset was preprocessed. Sensitivity, accuracy, and definiteness metrics were used to measure the success of the methods. Result: Compared to other ML algorithms used in the study, the GBC ML algorithm was found to be the most successful method in the classification of tumors with an accuracy of 99.12%. The XGB ML algorithm was found to be the lowest method with an accuracy rate of 88.10%. In addition, it was determined that the general accuracy rates of the 11 ML algorithms used in the study varied between 88-95%.Conclusion: When the results obtained from the ML classifiers used in the study are evaluated, the efficiency of the GBC algorithm in the classification of tumors is obvious. It can be said that the success rates obtained from 11 different ML algorithms used in the study are valuable in terms of being used to predict different cancer types.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3287
3297
https://journal.waocp.org/article_90310_cf7bd74db51492ae781704d383c21ece.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3287
Risk Factors for the Diagnosis of Lung Cancer in Poland: A Large-Scale, Population-Based Case-Control Study
Anna
Lewandowska
Institute of Healthcare, State School of Technology and Economics, 37-500 Jaroslaw, Poland.
author
Tomasz
Lewandowski
Institute of Technical Engineering, State School of Technology and Economics in Jaroslaw, 37-500 Jaroslaw, Poland.
author
Barbara
Zych
Institute of Health Sciences, Medical College of Rzeszow University, 35-310 Rzeszow, Poland.
author
Katalin
Papp
Faculty of Health, University of Debrecen, Sóstói út 2-4 4400 Nyíregyháza, Hungary.
author
Dana
Zrubcová
Faculty of Social Sciences and Health Care Constantine the Philosopher, University in Nitra, Kraskova 1 949 74 Nitra, Slovak Republic.
author
Serap
Ejder Apay
Faculty of Health Science, Midwifery Department, Ataturk University, Bölümü 25240 Erzurum, Turkey.
author
Małgorzata
Nagorska
Institute of Medical Sciences, Medical College of Rzeszow University, 35-310 Rzeszow, Poland.
author
text
article
2022
eng
Objective: Lung cancer is one of the most common and deadly malignant neoplasms. Currently, it is one of the main causes of cancer deaths worldwide. The study aimed to identify and evaluate patient characteristics, demographic and lifestyle factors that are associated with lung cancer at diagnosis. Methods: The study included 400 patients diagnosed with lung cancer and 400 within the control group. The research was based on a clinical, direct, individual, structured, in-depth and focused interview. Assessment of activity and BMI was used according to WHO recommendations, as well as the expert system. Results: The mean age of the patients was 74.53 ± 7.86 years, while in the control group 59.5 (7.93). There was a strong positive relationship between the incidence of tuberculosis and chronic obstructive pulmonary disease and the risk of lung cancer (p <0.001). The risk of lung cancer was significant in the case of smoking 20 or more than 20 cigarettes a day and smoking for more than 20 years (p = 0.01). Conclusions: Active and passive smoking, are a leading risk factor for lung cancer, which shows that understanding of the long-term and fatal effects of smoking is still very low in society. No significant correlation has been found between lifestyle and risk of lung cancer. However, there was a strong positive correlation between tuberculosis and chronic obstructive pulmonary disease and the risk of lung cancer. Occupation is a predisposing factor for lung cancer occurrence.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3299
3307
https://journal.waocp.org/article_90309_378a802a3b99fa0e943cc48bbf47d35a.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3299
Mesenchymal Stem Cells Treatment Aggravates Tumor Growth Regardless Its Route of Administration: An In vivo Study
Abeer
Mostafa
Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt.
author
shimaa
Mohamed Abdelsalam
Department
of Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt.
author
W
.s.sabbah
Department of Anatomy, Faculty of Medicine for girls, Al Azhar
University, Egypt.
author
Dina
Mekawey
Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt.
author
text
article
2022
eng
Objectives: to clarify the effect of MSCs in cancer growth and to detect whether the rout of administration (either locally inside the tumor tissue or systemic )could affect the outcome of treatment or not. Methods: Eighteen female mice were involved in the study. All mice were subcutaneously inoculated with Ehrlich tumor cells into the right flank. After three week of tumor growth; the mice were divided randomly in to three groups six mice for each ; group I: untreated Erlish tumor group; group II: Erlish tumor treated by local injection of 1 x106 MSCs/week inside the tumor tissue, group III: Erlish tumor treated by systemic injection of 1 x106 MSCs iv in tail vein/week. Tumor growth was recorded .After 4 weeks of stem cells injection, all rats were sacrificed by cervical dislocation and tumor tissues were collected for histopathological study. inflammatory cytokine TNF was assessed by ELISA, lncRNA MALAT ,NFKB and MMP2 genes expression were assessed by Quantitative RT-PCR. Results: Erlish tumor was developed as a well-defined capsule composed by connective tissue infiltrated by inflammatory and neoplastic cells surrounded the tumors. The tumor growth regarding size and weight of tumor tissue was significantly aggravated after both local and systemic treatment MSCs (p value =0.007, 0.001) respectively. Inflammatory cytokines TNF and NFKB were significantly elevated (p value <0.0001), lncRNA MALAT, MMP2 expressions were significantly induced (p value <0.0001), after MSCs treatment with more significant increase in those treated by local intratumor injection of MSCs compared to those treated by systemic MSCs(p value <0.0001). Conclusion: Ehrlich tumor model is feasible and easily monitored tumor model. Although MSCs have anti-inflammatory effect and the ability to regenerate the damaged tissue; it could aggravate tumor growth as it exploited by cancer cells for behave of tumor cells.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3309
3315
https://journal.waocp.org/article_90338_c4312028dd5b3cdd29fc0d24d776b480.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3309
Study on Preoperative Neutrophil/Lymphocyte (NLR) and Platelet/Lymphocyte Ratio (PLR) as a Predictive Factor in Endometrial Cancer
Teerapat
Muangto
Department of Obstetrics and Gynaecology, Faculty of Medicine, Thammasat University, Pathumthani, Thailand.
author
Karicha
Maireang
Department of Obstetrics and Gynaecology, Faculty of Medicine, Thammasat University, Pathumthani, Thailand.
author
Yenrudee
Poomtavorn
Department of Obstetrics and Gynaecology, Faculty of Medicine, Thammasat University, Pathumthani, Thailand.
author
Yuthadej
Thaweekul
Department of Obstetrics and Gynaecology, Faculty of Medicine, Thammasat University, Pathumthani, Thailand.
author
Awassada
Punyashthira
Department of Obstetrics and Gynaecology, Faculty of Medicine, Thammasat University, Pathumthani, Thailand.
author
Nopwaree
Chantawong
Department of Obstetrics and Gynaecology, Faculty of Medicine, Thammasat University, Pathumthani, Thailand.
author
Pattra
Wisarnsirirak
Department of Obstetrics and Gynaecology, Faculty of Medicine, Thammasat University, Pathumthani, Thailand.
author
Junya
Pattaraarchachai
Chulabhorn International College of Medicine, Thammasat University, Pathumthani, Thailand.
author
Komsun
Suwannarurk
Department of Obstetrics and Gynaecology, Faculty of Medicine, Thammasat University, Pathumthani, Thailand.
author
text
article
2022
eng
Objective: To evaluate predictive factors between serum inflammatory markers and malignancy potential of endometrium. Methods: This retrospective study was conducted at the gynecological oncology unit, department of obstetrics and gynecology, at the faculty of medicine of Thammasat University. The study period was from 2017 to 2020. Endometrial cancer and benign gynecologic disease cases who underwent hysterectomy (with or without adnexectomy) during the study period were recruited. Demographic characteristics, histopathology reports and serum markers were also collected. Results: The study included a study group of 49 participants with endometrial cancer and a control group consisting of 119 cases of benign uterine disease. The study group had statistically significantly higher mean ages, proportional menopausal status and instances of underlying diseases when compared with the control group. Neutrophil/Lymphocyte ratios (NLR) and Platelet/Lymphocyte ratios (PLR) could not meaningfully predict the malignant potential of endometrium in hysterectomy specimens. NLR and PLR were statistically associated with depth of myometrial invasion (MI) in endometrial cancer cases. NLR equal to or greater than 1.93 predicted MI more than half thickness with sensitivity, specificity, accuracy, positive (PPV) and negative predictive value (NPV) at a percentage of 83.3, 52.8, 37.0, 90.5 and 60.4, respectively. PLR equal to or greater than 134.95 predicted MI of greater than fifty percent thickness with sensitivity, specificity, accuracy, PPV and NPV at 75.0, 55.6, 36.0, 87.0 and 60.4 percent, respectively. Conclusion: NLR and PLR have positive associations with myometrial invasion of endometrial cancer.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3317
3322
https://journal.waocp.org/article_90305_8ba120d36dc11e2507c7e1e58aaa48cb.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3317
Efficacy of Short Hydration for Intermediate to High-Dose Cisplatin-Based Chemotherapy for Outpatients: SHORTCIS Trial
Chaichana
Chantharakhit
Division of Medical Oncology, Department of Internal Medicine, Buddhasothorn Hospital, Chachoengsao, Thailand.
author
Nantapa
Sujaritvanichpong
Division of Medical Oncology, Department of Internal Medicine, Buddhasothorn Hospital, Chachoengsao, Thailand.
author
text
article
2022
eng
Objective: Supporting data exists concerning short hydration to prevent cisplatin-induced nephrotoxicity. However, only a few studies exist. Further, data remains limited, comprising mostly retrospective data. Therefore, the study would like to evaluate the efficacy of short hydration using a prospective cohort study. Materials and Methods: This is a prospective cohort non-randomized controlled study in patients receiving intermediate to high doses of cisplatin. Short hydration was set as the intervention arm, while conventional hydration was set as the controlled arm. The consecutive estimates glomerular filtration rates (eGFR) were compared at baseline, Week 3, Week 6, Week 9, Week 12, and Week 15 for both groups by using multilevel regression analysis with the random-effects model with double adjustment (propensity score and confounding adjustment) was used. The trial was registered with the Thai Registry of Clinical Trials, SHORTCIS ThaiClinicalTrials.org, number TCTR20210128002. Results: 30 patients were registered. 14 were assigned to a short hydration group, while 16 were assigned to a conventional hydration group. The levels of consecutive eGFR of the group receiving short hydration were stable (regression coefficients 0.05), while the levels of consecutive eGFR of the group receiving conventional hydration were declined (regression coefficients -1.94). The multilevel regression analysis of consecutive eGFR between conventional group and short hydration group when adjusted for random-effects parameters and double adjustment were significantly different (p-value = 0.001). When analyzing the relationship of received short hydration, it could significantly reduce the risk of nephrotoxicity as well, i.e. acute kidney injury (odds ratio 0.06, 95%CI 0.003, 0.990, p-value 0.049). Conclusion: Short hydration was more efficient for preventing nephrotoxicity than conventional hydration protocols in patients receiving intermediate to high doses of cisplatin.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3323
3330
https://journal.waocp.org/article_90306_cb1b2e5278d331185aadd20dd687a2be.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3323
Association between PIK3CA Mutations in Blood and Tumor-Infiltrating Lymphocytes in Peruvian Breast Cancer Patients
Carlos
Castaneda
Department of Medical Oncology, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
author
Miluska
Castillo
Department of Research, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
author
Luis
Bernabe
Department of Research, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
author
Nancy
Suarez
Department of Research, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
author
Atocha
Romero
Department of Medical Oncology, Puerta de Hierro Majadahonda University Hospital, Madrid, Spain.
author
Joselyn
Sanchez
Department of Research, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
author
Ebert
Torres
Department of Pathology, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
author
Javier
Enciso
Laboratorio de Cultivo Celular e Inmunologia, Universidad Cientifica del Sur, Lima 15067, Peru.
author
Katherine
Tello
Department of Research, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
author
Nataly
Enciso
Laboratorio de Cultivo Celular e Inmunologia, Universidad Cientifica del Sur, Lima 15067, Peru.
author
Marco
Velarde
Department of Breast and Soft Tissues, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
author
Miguel
De La Cruz
Department of Breast and Soft Tissues, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
author
Jorge
Dunstan
Department of Breast and Soft Tissues, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
author
Jose
Cotrina
Department of Breast and Soft Tissues, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
author
Julio
Abugattas
Department of Breast and Soft Tissues, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
author
Miguel
Pinillos
Department of Breast and Soft Tissues, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
author
Katia
Roque
Department of Medical Oncology, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
author
Hugo
Fuentes
Department of Medical Oncology, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
author
Ebert
Poquioma
Department of Epidemiology, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
author
Henry
Guerra
Department of Pathology, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
author
Henry
Gomez
Department of Medical Oncology, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
author
text
article
2022
eng
Objective: To evaluate the relationship between circulating tumor DNA (ctDNA) presence and tumor features including tumor-infiltrating lymphocyte (TIL) levels in Peruvian breast cancer patients. Materials and Methods: This was a prospective study conducted at the Instituto Nacional de Enfemedades Neoplasicas, Peru. We evaluated level of TIL and PIK3CA mutations in ctDNA. Clinical characteristics, including outcome data, were collected from the patient file. Survival was calculated from the date of blood sample drawn to the event time. Data collected were analyzed using SPSS software version 25. Results: We analyzed plasma samples from 183 breast cancer patients. most cases were of Luminal-B (44.8%) phenotype and stage II (41.5%), and median stromal TIL was 30%. PIK3CA mutation in ctDNA was detected in 35% cases (most with E545K) and was associated with lower TIL level (p=0.04). PIK3CA in ctDNA tended to be associated with advanced stages (p=0.09) in the whole series and with higher recurrence rates (p=0.053) in the non-metastatic setting. Patients with presence of PIK3CA in ctDNA tended to have shorter survival (p=0.083). Conclusion: Presence of PIK3CA mutation in ctDNA was frequently found in our Peruvian breast cancer series, was associated with lower TIL levels and tended to predict poor outcomes.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3331
3337
https://journal.waocp.org/article_90307_f10e514bd68dc8a6c0da9d5f4526fb5b.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3331
The Relation of Work Ability and Return to Work Among Iranian Cancer Survivors
Mostafa
Ghasempour
Tabriz University of medical sciences Tabriz University of Medical Sciences, Tabriz, Iran.
author
Majid
Porabdolah
Tabriz University of medical sciences Tabriz University of Medical Sciences, Tabriz, Iran.
author
Azad
Rahmani
Tabriz University of medical sciences Tabriz University of Medical Sciences, Tabriz, Iran.
author
Javad
Dehghannezhad
Tabriz University of medical sciences Tabriz University of Medical Sciences, Tabriz, Iran.
author
Saeed
Mousavi
Tabriz University of medical sciences Tabriz University of Medical Sciences, Tabriz, Iran.
author
Simin
Sattarpour
Tabriz University of medical sciences Tabriz University of Medical Sciences, Tabriz, Iran.
author
text
article
2022
eng
Objective: Cancerous diseases are known to disrupt a person’s ability and inflict physical, psychological, financial, and social complications on the person, thereby challenging an individual’s returning to work. The aim of this study was to investigate the ability of cancer patients to work after returning to work. Methods: This descriptive-correlational study examined a total of 227 surviving cancer patients, having picked the participants through convenience sampling. Data were collected by the return to work and work ability index (WAI) questionnaires and analyzed by descriptive statistics and inferential statistics using SPSS software. Results: A total of 166 (73.2%) of the participants had returned to work after completing the basic treatment. The mean (standard deviation) of the work ability score was 29.52 (9.43), ranging from 9 to 43 while the average daily work hours dropped from 12.30 to 5.50. The chi-square test showed a significant relationship between the work ability score and the type of return to work. Moreover, the rank logistic regression analysis revealed that work ability was the most important predictor of return to work. Conclusion: Survivors of cancer face reduced working hours and limited ability to work after returning to work, and it is possible to facilitate the return to work in these patients by identifying their job needs in relation to their abilities and barriers of returning to work through the appropriate interventions.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3339
3346
https://journal.waocp.org/article_90308_46290c83af18f6f5cc46a435ae6b9a1b.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3339
The Immune-related ceRNA Network in Prognosis of Cervical Cancer
Hemavathi
Dhandapani
Department of Molecular Oncology, Cancer Institute [WIA], Dr. Krishnamurthi campus, 38, Sardar Patel road, Chennai, India.
author
Mayilvahanan
Bose
Department of Molecular Oncology, Cancer Institute [WIA], Dr. Krishnamurthi campus, 38, Sardar Patel road, Chennai, India.
author
Sabitha
Kesavan
Department of Molecular Oncology, Cancer Institute [WIA], Dr. Krishnamurthi campus, 38, Sardar Patel road, Chennai, India.
author
text
article
2022
eng
Background: Immunotherapy is gaining attention and it is being included as one of the treatment strategies for cancer patients. However, the molecular mechanisms of immune-related genes and their affinity for cervical cancer progression remain unclear. In this study, we have developed an immune-related competing endogenous RNA [ceRNA] network and assessed the tumour infiltrating immune cells towards the prognosis of cervical cancer. Methods: Differential RNA expression pattern between stages I and II-IV of cervical cancer patients from The Cancer Genome Atlas [TCGA] was analyzed. Immune-related ceRNA network based on the immune gene signatures were retrieved and their targets were predicted using miRwalk 3.0. CIBERSORT was employed to identify the immune cell types based on their respective transcripts. The prognostic significance of RNAs in the ceRNA network and immune cell subsets was analyzed. Results: Significant differences in 22 long non-coding RNAs [lncRNAs], 15 microRNAs [miRNAs], and 252 messenger RNAs [mRNAs] between stages I and II-IV of cervical cancer were observed. Further, we shortlisted the 49 immune-related mRNAs based on immune gene signature and predicted their target miRNAs and lncRNAs. A potential ceRNA network of 4 lncRNAs, 10 miRNAs, and 11 mRNAs had a strong correlation for prognosis. Out of 11 protein-coding immune mRNAs, IRF4 and AZGP1 had high degrees of interaction. In addition, the evaluation of immune cell subsets showed increased infiltration of M1 macrophages had better survival outcome. Conclusions: We have identified an immune-related ceRNA network based on differentially expressed transcripts between stages I and II-IV which may help predict the prognosis of cervical cancer.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3347
3354
https://journal.waocp.org/article_90314_3d26710e7cb443b1c721130ecb81e4c2.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3347
Estimation of Neural Tissue Mobility in Breast Cancer Survivors with Lymphedema
Sandeep
Shinde
Department of Musculoskeletal Sciences, Krishna College of Physiotherapy, Krishna Institute of Medical Sciences, Deemed to Be University, Karad, Maharashtra, India.
author
Devanshi
Joshi
Department of Musculoskeletal Sciences, Krishna College of Physiotherapy, Krishna Institute of Medical Sciences, Deemed to Be University, Karad, Maharashtra, India.
author
Sanjaykumar
Patil
Department of Obstetrics and Gynaecology,Krishna Institute of Medical Sciences Deemed to Be University, Karad, Maharashtra, India.
author
Piyusha
Pawar
Department of Musculoskeletal Sciences, Krishna College of Physiotherapy, Krishna Institute of Medical Sciences, Deemed to Be University, Karad, Maharashtra, India.
author
text
article
2022
eng
Background: Lymphedema in breast cancer survivors is a very common condition which progressively may lead to entrapment neuropathy. In lymphedema there is accumulation of fluid due to removal of lymph nodes which causes stretching of nerve fibres within the skin, compression on top of the nerve bundle leading to nerve entrapment. This will increase the neural mechanosensitivity and functional impairment of shoulder as a protective neural response to movement or traction. Methods: This study was carried out by assessing the total 72 breast cancer survivor women, with lymphedema. Out of 72, 28 of women underwent lumpectomy, 12 underwent quadrantectomy and 32 underwent unilateral mastectomy. These subjects were assessed for neural tissue mobility by taking pain assessment using visual analogue scale (VAS), range of motion (ROM) using goniometer, lymphedema measurement using an inch tape. The neural tissue mobility for median nerve, ulnar nerve and radial nerve was measured using limb tension test. Result: The result obtained from this study showed that neural tissue mobility was significantly impaired in breast cancer survivors with lymphedema. The result of the upper limb tension tests showed 32 women with mild lymphedema had median nerve affected on the involved side 54.1%, about 21 women had moderate lymphedema with 75% of women had median and 25% ulnar nerve affected with median nerve affected in majority of women. Only 19 women with severe lymphedema had all the three nerves affected. Conclusion: This study of women who have undergone surgical intervention for breast cancer concludes that there was significant amount of neural tissue impairment noted to mechanical provocation test post operatively after 6 months of surgery. The study suggests that severity of lymphedema was directly related to the nerves affected due to neural tissue impairment.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3355
3360
https://journal.waocp.org/article_90315_9bb1707a2de6bfe01c09ea2b0f59e48a.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3355
The Role of miRNA-182 and FOXO3 Expression in Breast Cancer
Noha
Kandil
Department of Chemical Pathology, Medical Research Institute, Alexandria University, Egypt.
author
Lamia
Kandil
Department of Pharmacology and Therapeutics, Faculty of Pharmacy, Pharos University, Egypt.
author
Radwa
Mohamed
Department of Pathology, Medical Research Institute, Alexandria University, Egypt.
author
Mohamed
Selima
Department of Surgery, Medical Research Institute, Alexandria University, Egypt.
author
Mohamed
El Nemr
Department of Cancer Management and Research, Medical Research Institute, Alexandria University, Alexandria, Egypt.
author
Abdelrahman Rafik
Barakat
MB BCh , October 6 University, Egypt.
author
Yasmine
Alwany
Department of Cancer Management and Research, Medical Research Institute, Alexandria University, Alexandria, Egypt.
author
text
article
2022
eng
Objective: evaluating the role of FOXO3 mRNA and mi RNA 182-5P expression levels in BC patients. Method: 25 Samples of breast cancer and paired samples of non-cancerous tissues from the same resected breast were obtained from 25 female patients suffering from breast cancer and examined and analyzed by real time PCR to detect the expression levels of FOXO3 mRNA and mi RNA 182-5P. Patients’ data were collected from patients medical records. Results: Foxo3 m RNA expression was down regulated in BC tissues (1.37± 1.96) as compared to control group (23.62 ± 54.39) and decreased FOXO3 expression was associated with larger tumor size (p= 0.046), late histopathological grading (p= 0.002), late TNM staging (<0.001) and increased miR-182 expression (p= 0.025). We found that expression level of miR-182 was significantly higher among breast cancer group (1.10±1.15) as compared to the control group (0.58±0.96 ) with p value = 0.017. We noted a significant increased expression associated with larger tumor size (p= 0.002), late histopathological grading (p= 0.008), late TNM staging (p= 0.002) and decreased FOXO3 expression (p= 0.025). A significant negative correlation between miR-182 and FOXO3 mRNA fold expression with r = - 0.447, and a p value of 0.025, this could be attributed to miRNA targeting FOXO gene. Coclusion: Down regulation of FOXO3 and up regulation of miR-182 expression was associated with advanced breast cancer. The negative correlation between miR-182 and FOXO3 mRNA could be attributed to miRNA targeting FOXO gene.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3361
3370
https://journal.waocp.org/article_90316_6f29d45bdb5f0523b0bb846a7e433854.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3361
Survival Analysis and Clinical Outcomes between Paclitaxel and Carboplatin Versus Carboplatin and Gemcitabine in Patients with Advanced-stage Non-small-cell Lung Cancer: A Single-center Cohort Study
Thitaya
Boonsong
Division of Pulmonology, Department of Internal Medicine, Hatyai Hospital, Songkhla, Thailand.
author
Sirikate
Usaha
Division of Pulmonology, Department of Internal Medicine, Hatyai Hospital, Songkhla, Thailand.
author
Narongwit
Nakwan
Division of Pulmonology, Department of Internal Medicine, Hatyai Hospital, Songkhla, Thailand.
author
Thidarat
Ruklerd
Department of Nursing, Hatyai Hospital, Songkhla, Thailand.
author
Phungern
Khongthong
Division of Medical Oncology, Department of Internal Medicine, Hatyai Hospital, Songkhla, Thailand.
author
Arunchai
Chang
Division of Gastroenterology, Department of Internal Medicine, Hatyai Hospital, Songkhla, Thailand.
author
text
article
2022
eng
Background: Palliative chemotherapy using platinum-based doublet chemotherapy was recommended as one of the standard treatments in patients with advanced-stage non-small cell lung cancer (NSCLC) with negative EGFR mutation. This study aimed to compare clinical outcomes between patients treated with paclitaxel and carboplatin (PC) and those treated with carboplatin and gemcitabine (CG). Methods: We conducted a retrospective cohort study comparing PC and CG at Hatyai Hospital between 2012 and 2019. The primary outcome was survival analysis, and the secondary outcome was chemotherapy-related adverse events, and the rate and reason for stopping chemotherapy.Result: The median overall survivals of both groups was comparable (9.0 months for the PC group and 9.6 months for the CG group; log-rank, p=0.287). The CG group had a higher incidence of adverse events (89.7% vs. 77.9%, p=0.010) and tended to have a lower rate of chemotherapy discontinuation (29.6% vs. 41.2%, p=0.080) than the PC group. In the multivariate analysis, female sex (odds ratio [OR]=0.351; 95% confidence interval [CI], 0.158-0.780; p=0.010) and higher performance status (OR=76.374; 95%CI, 32.533-179.295; p<0.001) were independent predictive factors for stopping chemotherapy. In the proportional hazards model, the factors associated with decreased survival included higher performance status (hazard ratio [HR]=1.939; 95%CI, 1.388-2.709; P<0.001) and discontinuation of chemotherapy (HR=2.572; 95%CI, 1.792-3.691; p=<0.001). Conclusion: These two platinum-based regimens had comparable effects on overall survival. The CG group had a higher incidence of chemotherapy-related adverse events, while the PC group had a marginally significantly higher rate of stopping chemotherapy from unacceptable adverse events and deterioration of patients’ clinical status.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3371
3378
https://journal.waocp.org/article_90317_7cef7511068533b4cd6110d989190b31.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3371
Low Dose Berberine Suppresses Cholangiocarcinoma Cell Progression as a Multi-Kinase Inhibitor
Sumalee
Obchoei
Division of Health and Applied Sciences, Faculty of Science, Prince of Songkla University, Songkhla 90110, Thailand.
author
Marutpong
Detarya
Department of Biochemistry, Center for Translational Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
author
Piyanard
Boonnate
Division of Hematopoiesis, Joint Research Center for Human Retrovirus Infection and Graduate School of Medical Sciences, Kumamoto University, Kumamoto, 860-0811 Japan.
author
Paksiree
Saranaruk
Department of Biochemistry, Center for Translational Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
author
Kulthida
Vaeteewoottacharn
Department of Biochemistry, Center for Translational Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
author
Panupong
Mahalapbutr
Department of Biochemistry, Center for Translational Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
author
Seiji
Okada
Division of Hematopoiesis, Joint Research Center for Human Retrovirus Infection and Graduate School of Medical Sciences, Kumamoto University, Kumamoto, 860-0811 Japan.
author
Sopit
Wongkham
Department of Biochemistry, Center for Translational Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
author
text
article
2022
eng
Background: Berberine (BBR), a natural isoquinoline alkaloid, possesses diverse pharmacological properties and anti-cancer effects that have been demonstrated in many in vitro and in vivo studies. In this study, the inhibitory effects and molecular mechanism of low dose BBR on EMT-induced cell migration, and invasion capability of cholangiocarcinoma (CCA) cell lines were demonstrated. Methods: The commercially available BBR chloride powder with purity ≥ 95% was used in this study. Effects of BBR on cell growth of two human CCA cell lines, KKU-213A and KKU-213B were measured using MTT assay. The progressive phenotypes-cell adhesion, migration, and invasion were evaluated using cell adhesion, wound healing, and Boyden chamber assays. Molecular docking analysis was performed to assess the possible binding mode of BBR against EGFR, Erk, STAT3 and Akt. The effects of BBR on the activations of EGF/EGFR and its downstream effectors were demonstrated using Western blotting. Results: BBR inhibited growth of CCA cells in a dose dependent manner. At sub-cytotoxic dose, BBR significantly inhibited cell adhesion, migration, invasion and decreased expression of vimentin, slug, and VEGFA of both CCA cell lines. Molecular docking suggested the simultaneous inhibitory activity of BBR on EGFR, Erk, STAT3 and Akt. The Western blot analyses revealed that upon the EGF/EGFR activation, BBR considerably attenuated the activations of EGFR, Erk, STAT3 and Akt. Conclusion: Low dose of BBR suppresses EMT and thus aggressiveness of CCA cells, in part by its multi-kinase inhibitor property on EGFR and its downstream pathways. BBR might be beneficial for therapy of human CCA.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3379
3386
https://journal.waocp.org/article_90319_498076efd7456dab74b415860b443340.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3379
Diffusion-Weighted MRI as Non-Invasive Diagnostic Tool for Rectal Cancer Aggressiveness and Correlation with KI-67 Expression in Tumor Tissue
Fadila
EL Sayed
Department of Radiodiagnosis, Faculty of Medicine For Girl, Al-Azhar University, Cairo, Egypt.
author
Eman
Nassef
Department of Internal
Medicine, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt.
author
Neamat
Abdelmageed
Department of Hepatogastroenterology and Infectious Diseases, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt.
author
Rania
Abdel Maqsoud
Department of Pathology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt.
author
Amany
Abosaif
Department of Pathology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt.
author
text
article
2022
eng
Background and aim: Apparent diffusion coefficient (ADC) was suggested as a prognostic marker in rectal carcinoma (RC). However, reported data are inconsistent. The present study aimed to assess the relation between ADC value and Ki-67 expression index and other pathological parameters in Egyptian RC patients. Materials and Methods: The study included 39 patients with newly diagnosed RC (non-mucinous adenocarcinoma). All patients underwent magnetic resonance imaging (MRI) scan by 1.5T magnet. Mean ADC value was calculated. Pathological features were assessed and Ki- 67 immunohistochemical expression was applied as a proliferative index (PI) biomarker. Results: It was shown that patients with T4 tumors had significantly lower ADC values when compared with patients with T2 and T3 (0.903 ± 0.24 versus 1.157 ± 0.31 and 0.971 ± 0.26 respectively, p<0.001). Also, patients with circumferential resection margin (CRM) involvement had significantly lower ADC values when compared with patients without (0.905 ± 0.24 versus 1.109 ± 0.30, p=0.036). Patients with T4 tumors expressed significantly higher ki-67 PI when compared with patients with T2 and T3 tumors (75.71 ± 5.14 versus 46.25 ± 5.18 and 75.71 ± 5.14 respectively, p<0.001). Pearson’s correlation coefficient identified a significant inverse correlation between ADC values and ki-67 PI (r=-367, p=0.027). Conclusion: ADC values of RC may reflect tumor staging and Ki-67 is closely related to the ADC value confirm this result.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3387
3391
https://journal.waocp.org/article_90320_ae98ac6ab9aca9bf3f9ea179458ed128.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3387
Association between Prestored Smartphone Monitored Physical Activity and the Risk of HPV Infection and Cervical Cancer
Jie
Xing
Department of Gynecological Oncology, The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer
Hospital), Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, China.
author
Tao
Zhu
Department of Gynecological Oncology, The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer
Hospital), Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, China.
author
Jiejie
Zhang
Department of Gynecological Oncology, The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer
Hospital), Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, China.
author
Wenjun
Yang
Department of Gynecological Oncology, The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer
Hospital), Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, China.
author
Ai-Jun
Yu
Department of Gynecological Oncology, The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer
Hospital), Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, China.
author
text
article
2022
eng
Objective: This study was to determine the prevalence of HPV in non-vaccinated women from East China, and the association between prestored smartphone monitored physical activity and the risk of human papillomaviruses (HPV) infection and cervical cancer. Methods: We retrospectively reviewed medical records of unvaccinated women received first-time cervical HPV screening in the Affiliated Cancer Hospital of University of Chinese Academy of Sciences between March 2018 and December 2019. HPV genotyping was examined by the GenoArray. Physical activity defined by any movements at speeds of 0.5-2 m/s was obtained from smartphones. We collected prestored physical activity data for 6 months prior to the HPV screening. Logistic regression models were applied to determine the association between physical activity and the risk of HPV infection and cervical cancer. Results: A total of 11,730 women were initially included. Women with cervical cancer had significantly higher prevalence of infection with any high-risk (HR) HPV, or with individual HPV16, 18, 31, 33, 45, 52 and 58. Among them, 896 controls and 289 cervical cancer women had information of smartphone monitored physical activity. Multivariate logistic regression analysis showed that more daily physical activity time (or distance) was a protective factor for infection with any HR HPV, or infection with HPV16, but not other individual HPVs. Increased age, less physical activity time (or distance), and infection with any HR HPV (16, 18, 31, 52 and 58) were associated with a significantly increased risk of cervical cancer. In contrast, obesity was not associated with risk of HPV infection and cervical cancer. Conclusion: The high prevalence of HPV infection in unvaccinated women highlights the importance of prevention. More daily physical activity time (or distance) may help to reduce the risk of HPV infection and cervical cancer. Smartphone monitoring is an effective tool for recording physical activity.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3393
3404
https://journal.waocp.org/article_90342_c3faf6c62c7144ba2eaa89a9de1af421.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3393
P16/Ki-67 Dual Staining in Positive Human Papillomavirus DNA Testing for Predictive Diagnosis of Abnormal Cervical Lesions in Northeastern Thai Women
Sirinart
Aromseree
Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
author
Weerayut
Wongjumpa
HPV & EBV and
Carcinogenesis Research Group, Khon Kaen University, Khon Kaen, 40002, Thailand.
author
Tipaya
Ekalaksananan
Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
author
Amornrat
Temtanakitpaisan
Department of Obstetrics and Gynecology,
Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
author
Pilaiwan
Kleebkaow
Department of Obstetrics and Gynecology,
Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
author
Sawarot
Srisathaporn
Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
author
Panwad
Tongchai
HPV & EBV and
Carcinogenesis Research Group, Khon Kaen University, Khon Kaen, 40002, Thailand.
author
Chamsai
Pientong
Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
author
text
article
2022
eng
Objective: Cervical cancer screening can effectively reduce new cervical cancer cases, including in Thailand. The abnormal results are subsequently referred for colposcopy. To avoid unnecessary colposcopy, an efficient triage is still needed for validation. This study aimed to investigate the overall positivity of cytology-based screening, HPV detection, and p16/Ki-67 dual staining and evaluate different triage strategies for predictive diagnosis of abnormal cervical lesions in northeastern Thailand. Methods: Cervical cells were collected from 191 women who came for cervical screening in the gynecological outpatient department during March 2019-February 2020. Pap smear samples were classified into 6 groups including 17 atypical glandular cells (AGC), 21 atypical squamous cells of undetermined significance (ASC-US), 7 atypical squamous cells - cannot exclude HSIL (ASC-H), 26 low-grade squamous intraepithelial lesions (LSILs), 19 high-grade SILs (HSILs) and 101 no squamous intraepithelial lesion (noSIL). Polymerase chain reaction (PCR) was performed for HPV DNA detection. HPV genotyping was determined by reverse line blot hybridization. P16/Ki-67 dual staining was performed by using CINtec PLUS Cytology kit. Biopsies from abnormal screening were collected for surgical pathology classification. Results: High-risk HPV (HR-HPV) infection was 2.97%, 29.41%, 38.10%, 57.14%, 46.15% and 84.21% in noSIL, AGC, ASC-US, ASC-H, LSIL and HSIL cytology respectively. P16/ Ki-67 in noSIL, AGC, ASC-US, ASC-H, LSIL and HSIL was 0.99%, 5.88%, 9.52%, 42.86%, 26.92% and 63.16%, respectively (P-value < 0.001). Among p16/Ki-67 positive cases, 96.15% (25/26) were infected with HPV and 84.62% (22/26) were HR-HPV. The overall positivity of each and co-testing between cytology or HPV DNA testing or p16/Ki-67 dual staining was evaluated. In each cervical lesion, primary HPV DNA testing showed the highest sensitivity, but low specificity. The combined all HPV/HR-HPV with p16/Ki-67 detection increased the specificity of abnormal cervical lesions. Conclusion: P16/Ki-67 dual stain cytology in HPV-positive women performs well for diagnosis of abnormal cervical lesions and should be considered for management of HPV-positive women to avoid unnecessary colposcopy referrals.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3405
3411
https://journal.waocp.org/article_90321_23d8d8a3e9b97b634710979612154901.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3405
Full Compliance of Adjuvant Endocrine Therapy Is Associated with Higher Disease-Free Survival in Hormone Receptor-Positive and HER2-Negative Chinese Breast Cancer Patients with First Tumor Recurrence
Zhensheng
Li
Department of Radiation Oncology, the Fourth Hospital of Hebei Medical University, Shijiazhuang, China.
author
Yunjiang
Liu
Department of Breast
Surgery, the Fourth Hospital of Hebei Medical University, Shijiazhuang, China.
author
Yue
Li
Department of Radiation Oncology, the Fourth Hospital of Hebei Medical University, Shijiazhuang, China.
author
Yuguang
Shang
Department of Radiation Oncology, the Fourth Hospital of Hebei Medical University, Shijiazhuang, China.
author
Kaiye
Du
Department of Radiation Oncology, the Fourth Hospital of Hebei Medical University, Shijiazhuang, China.
author
Xiaohui
Ji
Department of Ultrasound, the Fourth Hospital of
Hebei Medical University, Shijiazhuang, China.
author
Huina
Han
Department of Radiation Oncology, the Fourth Hospital of Hebei Medical University, Shijiazhuang, China.
author
Jun
Zhang
Department of Radiation Oncology, the Fourth Hospital of Hebei Medical University, Shijiazhuang, China.
author
text
article
2022
eng
Purpose: To characterize the compliance status of adjuvant endocrine therapy (aET) and its relationship with disease-free survival (DFS) in hormone receptor-positive (HR+) and HER2-negative (HER2−) in Chinese breast cancer (BC) patients with first tumor recurrence. Methods: All women with primary unilateral stage I – III HR+HER2− BC and first tumor recurrence in 2008 - 2018 at our institution were identified. Full (vs. none/partial) compliance of aET was classified from records. Multivariate Cox regression estimated the hazard ratio (HR), its 95% confidence interval (CI), and p value. DFS. Covariates included age, T stage, N stage, pathology, tumor grade, LVI, chemotherapy, radiotherapy. Results: A total 258 patients had average age 47.4 years at BC diagnosis and median DFS 31.7 months. Patients with ipsilateral (contralateral) region and organ recurrence were 47.7% (19.8%) and 71.9%. Compared to the patients with none/partial compliance of aET, the full compliance patients (54.3% ) had a higher DFS (median 35.0 vs. 25.2 months, p=0.009). Multivariate analysis showed that the full compliance of aET was associated with a lower HR 0.614 (95%CI 0.467 – 0.807, p<0.001) on recurrence. Early discontinuation (67.5%, 56/83) due to the drug side effects was the top reason for partial compliance of aET. Conclusions: Full compliance of aET was quite low in Chinese HR+HER2− BC patients. However, it was associated with a 38.6% lower risk of first tumor recurrence. To search for effective tools to improve the compliance of aET in this population should be stressed.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3413
3420
https://journal.waocp.org/article_90322_76898e8071711b7de6e322c6ef3c3192.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3413
Triplet (FOLFOXIRI) Versus Doublet (FOLFOX or FOLFIRI) Regimen as First Line Treatment in Metastatic Colorectal Carcinoma, a Prospective Phase II, Randomized Controlled Trial
Khaled
Khalil
Department of Clinical Oncology, Kasr El Ainy Hospital, Cairo University, Cairo, Egypt.
author
Hanan
Musallam
Department of Clinical Oncology, Kasr El Ainy Hospital, Cairo University, Cairo, Egypt.
author
Mohamed
Hassan
Department of Clinical Oncology, Kasr El Ainy Hospital, Cairo University, Cairo, Egypt.
author
Inas
Mahmoud
Department of Clinical Oncology, Kasr El Ainy Hospital, Cairo University, Cairo, Egypt.
author
text
article
2022
eng
Background: The outcomes of treatment of metastatic colorectal cancer (mCRC) is still unsatisfactory. Several trials approved that, the upfront treatment with triplet regimen included fluorouracil, leucovorin, irinotecan and oxaliplatin improved the outcomes of patients with metastatic disease as compared to standard doublet regimen. The objective of our study is evaluating the impact of upfront treatment with triplet (FOLFOXIRI) regimen on both oncological outcomes (response rate and survival) and patients’ tolerability in comparison to the standard doublet regimen. Methods: We randomly enrolled 64 patients with a newly diagnosed unresectable mCRC to receive either FOLFOXIRI (experimental arm) or FOLFIRI or FOLFOX4 (control arm) biweekly up to 12 cycles. The primary endpoints are overall response rate (RR) and patients’ tolerability. The secondary endpoints are the progression free and overall survival. Result: There was a significantly increase in RR (59% vs 37%) and complete remission rate (CR) (6.3% and 3.1%, respectively (P = 0.045) for the triplet therapy group compared to control group. Consequently, an increased rate of secondary resection of metastasis (21.9% vs 3.1% respectively; P=0.023). The FOLFOXIRI regimen was associated with higher rate of grade 3/4 toxicity but not statistically significant except febrile neutropenia (6.2%; P=0.03). There was numerical prolongation in the median PFS in the FOLFOXIRI group on compared to control group but not significantly (9 versus 8 months; P=0.11). The median OS was 20 and 22 months in FOLFOXIRI arm and control arm respectively with no statistically significant difference (P=0.57). Conclusion: FOLFOXIRI had a higher efficacy and higher conversion rate to secondary resection over the doublet regimen as an upfront treatment option, coupled with a manageable adverse event, but failed to improve the survival outcomes.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3421
3429
https://journal.waocp.org/article_90323_d8738f885ffb4672018b22c9a3c9450b.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3421
Cigarette Display Regulation At Point Of Sales (POS) And Its Impact On Cigarette Sales
Evina
Widianawati
Health Information Management Program, Faculty of Health, Dian Nuswantoro University, Semarang, Indonesia.
author
Faik
Agiwahyuanto
Health Information Management Program, Faculty of Health, Dian Nuswantoro University, Semarang, Indonesia.
author
Widya
Wulan
Public Health Program, Faculty of Health Sciences, Universitas Dian Nuswantoro, Semarang, Indonesia.
author
Adian
Khoironi
Health Information Management Program, Faculty of Health, Dian Nuswantoro University, Semarang, Indonesia.
author
abdillah
ahsan
Faculty of Economy and Business, Universitas Indonesia.
author
text
article
2022
eng
Objective: This study aims to determine the impact of cigarette display settings on cigarette sales in the stores in Semarang City.Methods: The research was conducted in August-October 2020 using a quasi-experimental study approach. It was conducted by setting the cigarette display in 3 methods where (1) the cigarette display was opened for 35 days in 5 stores, (2) the cigarettes display was covered with cloth for a pre and post-intervention period of 15 and 30 days, respectively, at five stores and (3) the cigarette display was hidden for a pre and post-intervention period of 15 and 30 days, respectively, at three stores. Cigarette sales were recorded based on how many packs or sticks were sold per day, and a visit to the store was made every two or three days without prior notification to the store owner to ensure the cigarette display was appropriate and recorded data. The sales data was descriptively evaluated using a paired t-test with a 95% confidence level. Result: The results showed that one hidden cigarette display store (R3-1) experienced a significant decrease in sales, three hidden/covered display stores had a less significant decrease in cigarette sales and four hidden/covered display stores didn’t have any decline in cigarette sales while the average decline in cigarette consumption in stores was -1.4packs/day. From the analysis of sales per day and the paired pre-post intervention test, the store with a covered display showed no difference in the pre-post intervention sales, while the stores with hidden displays showed differences. Conclusion: Thus, covered displays did not affect stores and cigarette sales, while stores with hidden displays showed differences in sales.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3431
3435
https://journal.waocp.org/article_90324_9acd337af68f37ff47d187dc5f36187c.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3431
The Effect of LL37 Antimicrobial Peptide on FOXE1 and lncRNA PTCSC 2 Genes Expression in Colorectal Cancer (CRC) and Normal Cells
Mustafa
Al-Darraji
Department of Biology, College of Science, University of Al-Anbar, Iraq.
author
Saade
Jasim
Medical Laboratory Techniques Department, Al-maarif
University College, Iraq.
author
Omer
Salah Aldeen
Al-maarif University College, Medical Laboratory Techniques Department, Anbar, Ramadi, Iraq..
author
Abdolmajid
Ghasemian
Noncommunicable Diseases Research Center, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran.
author
Mohammed
Rasheed
Applied
Sciences Department, University of Technology, Iraq.
author
text
article
2022
eng
Objective: As an innate immune system component, antimicrobial peptides (AMPs) exert various effects, such as anticancer properties. This study aimed to evaluate the LL37 AMP anticancer effect against colorectal cancer (CRC) cells and the expression of FOXE1 and lncRNA PTCSC2 genes. Methods: The LL37 AMP was purchased from GenScript USA, Inc. Various CRC cell lines (HCT-116, HT29, WiDr, and SW742) were cultured in the DMEM medium. Various concentrations ranging from (5-400) μg/mL of LL37 AMP were prepared, added to cell cultures, and incubated for (24 and 48) hours. A nontoxic level of 30 µg/mL of LL37 was investigated for FOXE1 and lncRNA PTCSC2 gene expression. Results: At 24 hours, the (50 and 90) % lethal concentrations of LC50 and LC90, respectively, of LL37 against NCM460 normal cells were (640 and > 640) g/mL. Additionally, these values at 48 hours included (160 and > 640) µg/mL, respectively. After 24 hours of treatment, the LC50 and LC90 of LL37 AMP against CRC cell lines included (20 and 200) µg/mL. The LC50 and LC90 of the LL37 at 48 hours included (20 and 50) µg/mL and at 72 hours. they included (~10 and 40) μg/mL, respectively. FOXE1 but not the PTCSC 2 gene expression was significantly higher in CRC cells than normal cells (NCM460 and HaCaT). The LL37 AMP significantly decreased FOXE1 gene expression by 1.95-fold in CRC cells (p < 0.001). Conclusion: The FOXE1 gene can be considered a biomarker of CRC development. The expression of FOXE1 but not the PTCSC2 gene was significantly affected by the LL37 AMP. The effects of LL37 AMP against CRC cells were time and dose-dependent. Future studies are warranted to verify these effects.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3437
3442
https://journal.waocp.org/article_90325_3ed1757a7dbbeef21a5cb0530b13a9d0.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3437
LINE-1 and Alu Methylation in hrHPV-Associated Precancerous Cervical Samples
Arkom
Chaiwongkot
Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand.
author
Supranee
Buranapraditkun
Division of Allergy and Clinical Immunology, Department of Medicine, King Chulalongkorn Memorial Hospital, Faculty of Medicine, Chulalongkorn University, Thai Red Cross Society, Bangkok 10330, Thailand.
author
Suthipong
Chujan
Laboratory of Pharmacology, Chulabhorn Research Institute, Bangkok 10210, Thailand.
author
Nakarin
Kitkumthorn
Department of Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok 10400, Thailand.
author
text
article
2022
eng
Objective: This study was conducted to determine global DNA methylation patterns in cervical cells cytologically identified as atypical squamous cells of unknown significance (ASCUS) with a normal, LSIL, or HSIL histopathological result. Methods: Methylation patterns of long interspersed nuclear elements (LINE-1) and short interspersed element (Alu) sequences were assessed using the combined bisulfite restriction analysis (COBRA) method in cervical samples with cytology-diagnosed cervical lesions. Results: In cervical precancerous lesions with hrHPV positive, the percentage of overall (mC) and mCmC LINE-1 methylation levels showed a stepwise increase from hrHPV positive normal to HSIL with significant differences (p<0.001). However, both methylation levels were significantly higher in hrHPV negative normal than in hrHPV positive normal (p<0.001). The overall (mC) Alu methylation in hrHPV positive LSIL and HSIL was lower than in hrHPV positive normal, with a significant difference (p<0.05). Remarkably, the percentage of uCmC and mCuC of LINE-1 and Alu in three different hrHPV positive cervical lesions showed a stepwise decrease from hrHPV positive normal, LSIL and HSIL, respectively. Furthermore, receiver operating characteristic (ROC) curve analyses revealed that the LINE-1 mC and mCmC patterns have high sensitivity and specificity for distinguishing HSIL from normal/LSIL in hrHPV positive cases at the appropriate cutoff levels. Conclusion: We have demonstrated the LINE-1 and Alu methylation data in normal and premalignant cervical epithelia. LINE-1 hypomethylation was found in hrHPV positive normal cells, with lower methylation levels associated with cancer features. In cytologically diagnosed Atypical Squamous Cells of Unknown Significance (ASCUS), the levels of mC and the mCmC pattern could be utilized in concert with hrHPV detection to classify the ASCUS sample prior to colposcopy.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3443
3448
https://journal.waocp.org/article_90326_36915db57a5f222fe168e6da828bd37e.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3443
TNF-α-308A allele Carrier Induced to Development of Chronic Lymphocytic Leukemia in Sudanese Population at Earlier Age
Ameen
Basabaeen
Department of Hematology, Faculty of Medical Laboratory Sciences, Al Neelain University, Khartoum, Sudan.
author
Enaam
Abdelgader
Department of Pathology, Faculty of Medicine, Al Neelain University, Khartoum, Sudan.
author
Ebtihal
Ahmed Babekir
Department of Hematology, Faculty of Medical Laboratory Sciences, Al Neelain University, Khartoum, Sudan.
author
Nour
Abdelateif
Department of Hematology, Faculty of Medical Laboratory Sciences, Al Neelain University, Khartoum, Sudan.
author
Sadia
Osman Abdelrahim
Department of Hematology, Faculty of Medical Laboratory Sciences, Al Neelain University, Khartoum, Sudan.
author
Awadalkareem
Awadalkareem Omer
Department of Microbiology, Faculty of Medical Laboratory Sciences, Al Neelain University, Khartoum, Sudan.
author
Osama
Altayeb
Flow Cytometry Laboratory for Leukemia & Lymphoma Diagnosis, Khartoum, Sudan.
author
Eman
Fadul
Flow Cytometry Laboratory for Leukemia & Lymphoma Diagnosis, Khartoum, Sudan.
author
Ibrahim
Ibrahim
Department of Hematology, Faculty of Medical Laboratory Sciences, Al Neelain University, Khartoum, Sudan.
author
text
article
2022
eng
Background: several studies have been performed to investigate the association of TNF-α-308G>ASNP and CLL susceptibility However, the results are inconsistent. This study aimed to investigate the association between TNF-α-308G>ASNP of the TNF-α gene and CLL risk in the Sudanese population and correlated genotypes with clinicopathological features. Methods: A case-control study was conducted in Khartoum state, during the period from April 2017 to April 2018, involved 110 CLL patients and 50 healthy volunteers. Physical examination, Complete Blood Count, and immunophenotype were performed in all patients to confirm the diagnosis. Clinical staging such as Rai and Binet were studied. CD38 and ZAP70 were performed by Flow Cytometry. Blood samples were collected from all participants; DNA was extracted by using ANALYTIKJENA Blood DNA Extraction Kit and analyzed TNF-α-308G>ASNP by using AS-PCR. The statistical analysis was performed using SPSS. Results: TNF-α-308G>A genotype frequencies were GG (10.0%), GA (87.3%), and AA (2.7%) among the CLL patients, and GG (14.0%), GA (80.0%), and AA (6.0%) in the control group. The comparison of CLL patients with the control group did not show any statistically significant relationship for the genotypic and allelic frequencies. Furthermore, no association was observed between the TNF-α-308G>ASNP and gender, hematological parameters, clinical stages systems, CD38 expression, and ZAP-70 expression. The presence of theTNF-α-308Aallele was associated with a lower mean age. Conclusions: These results indicate that TNF-α-308G>A genotypes are not involved in the predisposition to the development of CLL. TNF-α-308A allele carrier induced to development of CLL at an earlier age.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3449
3455
https://journal.waocp.org/article_90327_a0a96a2e41f670ba84103b8f5c35fe4a.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3449
Metabolite Profiling and Biological Activity Assessment of Casuarina equisetifolia Bark after Incorporating Gold Nanoparticles
Wael Mahmoud
Aboulthana
Biochemistry Department, Biotechnology Research Institute, National Research Centre, Dokki, Giza, Egypt.
author
Esraa
Refaat
Pharmacognosy
Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, Dokki, Giza, Egypt.
author
Sally
Khaled
Pharmacognosy
Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, Dokki, Giza, Egypt.
author
Noha
Ibrahim
Microbial
Biotechnology Department, Biotechnology Research Institute, National Research Centre, Dokki, Giza, Egypt.
author
Ahmed
Youssef
Packaging Materials
Department, National Research Centre, Dokki, Giza, Egypt.
author
text
article
2022
eng
Objective: Casuarina equisetifolia bark is rich in various active metabolites and selected to be studied due to limitation of the synthetic antioxidants that have adverse side effects. The present study aimed to enhance efficiency of the most effective extract by incorporating gold nanoparticles (Au-NPs). Methods: The phytochemical and biological measurements were carried out in total methanolic extract and its successive fractions. Moreover, these measurements were assayed in the most effective extract after incorporating Au-NPs. Results: The study revealed that total methanolic extract exhibited the highest biological and cytotoxic activities as compared to other fractions. Therefore, it is considered as good candidate for nano-extract preparation. The methanolic extract incorporated with Au-NPs showed higher antioxidant, scavenging and cytotoxic activities in addition to higher inhibitory effect against α-amylase activity as compared to native extract itself. To pinpoint active agents in total methanolic extract, the secondary metabolite profiling via HPLC-MS showed that 33 and 17 metabolites were annotated in the extract before and after incorporating Au-NPs, respectively. The median lethal dose (LD50) showed that gold total methanolic nano-extract is safer than total methanolic extract. Conclusion: This study concluded that total methanolic C. equisetifolia bark extract is a valuable bioresource to synthesize an eco-friendly Au-NPs with health-enhancing effect as antioxidant, antidiabetic and cytotoxic agents. The present study is considered as the first report on utilization of C. equisetifolia bark in synthesis of Au-NPs by mean of green nanotechnology and investigation of its biological activity in relation to its metabolite fingerprint.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3457
3471
https://journal.waocp.org/article_90328_7a283b95d3fc6095b1639941945649e9.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3457
Constipation Severity and Quality of Life among Patients with Cancer Who Received Prophylactic Laxatives: Quasi-Experimental Study
Muayyad
Ahmad
Clinical Nursing Department, School of Nursing, the Universe of Jordan, Amman, Jordan.
author
Mohammad
Alnaeem
Palliative Care and Pain Management, School of Nursing, Al-Zaytoonah University of Jordan, Amman, Jordan.
author
text
article
2022
eng
Background: Prophylactic laxatives were associated with decreasing the incidence of Opioid-induced constipation among patients with cancer. This study aims to evaluate the effectiveness of early prophylactic laxatives therapy on the severity of constipation and quality of life among patients with cancer receiving opioids. Methods: Using a quasi-experimental design with 64 patients assigned to control group and 66 patients assigned to intervention group. The final sample was composed from 112 patients (57 in control group and 55 in intervention group), who were selected from an oncology clinic. Patients in the intervention group have received prophylactical laxatives The intervention included an oral colonic stimulant laxative (i.e., Bisacodyl, Dose= 3 tab/ day and/or Senna 6.8 mg twice daily) and an oral colonic osmotic laxative (i.e., Lactulose, Dose = 15 ml three times per day). Patients in the control group continue to receive their routine care without laxatives. Results: Patients in the intervention group have reported a significant reduction in the severity of constipation symptoms at eight weeks post the intervention (p < 0.001). Furthermore, the patients in the intervention group have revealed a significant improvement in their quality of life (QoL) (p< 0.001). Conclusions: Patients with cancer need to use the first line of laxatives as prophylactic alongside with opioids to minimize the severity of Opioid-induced constipation symptoms and to enhance the QoL.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3473
3480
https://journal.waocp.org/article_90329_f487339a9006f1d49bb6f6e8e1385f93.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3473
Investigating the Role of Glutathione S- Transferase Genes, Histopathological and Molecular Subtypes, Gene-Gene Interaction and Its Susceptibility to Breast Carcinoma in Ethnic North- Indian Population
Priyanka
Gautam
Department of Biochemistry, University of Allahabad, Prayagraj, India.
author
Zainab
Feroz
Department of Biochemistry, University of Allahabad, Prayagraj, India.
author
Sonia
Tiwari
Department of Radiation Oncology, Kamala Nehru
Memorial Hospital, Prayagraj, India.
author
Sivakumar
Vijayraghavalu
Department of Life Science (Zoology), Manipur Central University (A Central University),
Imphal, India.
author
Girish
Shukla
Department of Biological Sciences and Center for Gene Regulation in Health and Disease, Cleveland State
University, Cleveland, OH, 44115, USA.
author
Munish
Kumar
Department of Biochemistry, University of Allahabad, Prayagraj, India.
author
text
article
2022
eng
Background: Breast Cancer (BC) is a genetically and clinically heterogeneous disease including complex interactions between gene-gene and gene-environment components. This study aimed, to explore whether the Glutathione S- transferase (GSTs) gene polymorphism has role in BC susceptibility. We further evaluated the frequency of four subtypes of BC based on molecular classification followed by microscopic histological analysis to study the grades of invasive ductal carcinoma (IDC). Materials and Method: Polymorphism in GST genes in North-Indian BC patients was assessed by multiplex-PCR and PCR-RFLP methods. 105 BC patients and 145 healthy controls were enrolled for this study. Data was analyzed by calculating the odds ratio (OR) and 95% CI from logistic regression analyses. Results: Our findings revealed that GSTM1 null genotype (OR = 2.231; 95% CI = 1.332–3.737; p-value= 0.002) is significantly associated to BC risk in ethnic North- Indian population. However, the risk for BC susceptibility in North–Indians does not appear to be associated with GSTT1 null genotype. The GSTP1 (Val/Val) genotype (OR=1.545; CI=0.663-3.605; p-value= 0.314) was also found to be susceptible for BC risk. Combination of three high risk GST genotypes association exhibiting gene-gene interaction further confirmed the increased risk to BC in this region. Conclusions: The results of present study indicated that polymorphism in GSTM1 and rs1695 of GSTP1 genes may influence BC development among North-Indian women. Thus, the screening of GSTM1 and GSTP1 gene should be recommended for the earlier investigation for BC as a precautionary measure.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3481
3490
https://journal.waocp.org/article_90330_a4160444106a8838acfda14dd4854485.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3481
The Importance of Skin Health Promotion for Children: Care with Makeup Use and Skin Cancer Prevention
Flavia
Teixeira
Pharmacology and Pharmacometry Laboratory, Federal University of Pampa, Uruguaiana, Brazil.
author
Felipe
Carvalho
Pharmacology and Pharmacometry Laboratory, Federal University of Pampa, Uruguaiana, Brazil.
author
Camila
Pacheco
Pharmacology and Pharmacometry Laboratory, Federal University of Pampa, Uruguaiana, Brazil.
author
Khayan
Pimentel
Pharmacology and Pharmacometry Laboratory, Federal University of Pampa, Uruguaiana, Brazil.
author
Marcelo
Gomes
Pharmacology and Pharmacometry Laboratory, Federal University of Pampa, Uruguaiana, Brazil.
author
Sandra
Haas
Pharmacology and Pharmacometry Laboratory, Federal University of Pampa, Uruguaiana, Brazil.
author
text
article
2022
eng
Background: Numerous pathogenic complications affect the skin and are preventable, such as skin cancer, microbial diseases, dermal irritations, and anaphylaxis. In this context, the correct use of skin products, including sunscreens and child makeup, is important for promoting skin health and preventing adverse health conditions. Objective: This study aimed to use educational and playful activities to promote skin health for students. Methods: This project was development in a municipal elementary school (Rio Grande do Sul State, Brazil). The interventions were divided into three moments. In the first day, a questionnaire was applied to find out the students’ previous knowledge about photoprotection. On the second day, an intervention lecture was held addressing issues related to photoprotection and the use of makeup. Finally, we played educational and ludic games and after, the questionnaire was reapplied. This was done to evaluate these actions’ effectiveness regarding photoprotection and record their habits by applying a structured questionnaire at the beginning and end of the activities. Results: Students received positively and interacted significantly during all activities performed. Regarding the impact of this study, we observed that ten times more students considered using sunscreen as something important at the end of the project, as only 8.16% of participants knew what skin cancer was at the beginning of the experiment. After the educational activities, this number rose to 72.37%, and 92.86% of girls reported wearing makeup, with more than half being expired or unlabeled and only 21.6% being appropriate for child use. Conclusion: The measures demonstrated effectively improve students’ level of information regarding skin cancer prevention and indicated that inappropriate habits concerning makeup use in childhood are quite common, demonstrating the importance of educational interventions for children, since can improve your health.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3491
3499
https://journal.waocp.org/article_90331_bbd81c7ef3d5826d9fd05820a41f4df0.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3491
Correlations among KRAS Mutation, Microsatellite Instability, and 18F-FDG Uptake in Colon Cancer
Sun Seong
Lee
Department of Nuclear Medicine, Busan Paik Hospital, University of Inje College of Medicine, Busan, Republic of Korea.
author
Su Jung
Choi
Department of Nuclear Medicine, Busan Paik Hospital, University of Inje College of Medicine, Busan, Republic of Korea.
author
Ji sun
Park
Department of Nuclear Medicine, Busan Paik Hospital, University of Inje College of Medicine, Busan, Republic of Korea.
author
text
article
2022
eng
Objective: This study aimed to evaluate the correlation of the maximum standardized uptake value (SUVmax) with the Kirsten ras sarcoma viral oncogene (KRAS) mutation and microsatellite instability (MSI) status in colon cancer. Methods: This retrospective study included 195 patients with colon cancer who underwent 18F-fluorodeoxyglucose positron emission tomography (18F-FDG PET/CT) before surgery between January 2014 and December 2017. All patients underwent KRAS mutation and MSI analyses using surgical specimens of the primary tumor. The associations of SUVmax with KRAS mutation and MSI were analyzed. Results: The SUVmax differed significantly between the microsatellite stable (MSS) and MSI groups (14.5 ± 7.0 vs. 19.1 ± 10.9; P = 0.0249), and between the KRAS wild-type and KRAS mutation groups (14.1 ± 7.6 vs. 17.5 ± 7.9; P = 0.0017). Conclusions: SUVmax obtained using 18F-FDG PET/CT showed significant differences in relation to KRAS mutation and MSI status. 18F-FDG PET/CT could be used as a supplemental modality for assessing KRAS mutations and MSI status in colon cancer.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3501
3506
https://journal.waocp.org/article_90343_4e01c46eaeea4f2810f4b22b20189a0c.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3501
Cancer Stem Cells as a Prognostic Biomarker and Therapeutic Target Using Curcumin/ Piperine Extract for Multiple Myeloma
Sara
Mekkawy
Molecular Biotechnology program, Faculty of Science, Helwan University, Cairo, Egypt.
author
Mohga
Abdalla
Chemistry Department, Faculty of
Science, Helwan University, Cairo, Egypt.
author
Mohamed
Omran
Chemistry Department, Faculty of
Science, Helwan University, Cairo, Egypt.
author
Naglaa
Hassan
Clinical Pathology Department, National Cancer Institute, Cairo University, Egypt.
author
Raafat
Abdelfattah
Medical Oncology Department, National cancer institute, Cairo University, Egypt.
author
Ibrahim
Abdel-Salam
Tumor Biology Department, National Cancer
Institute, Cairo University, Egypt.
author
text
article
2022
eng
Background: Multiple myeloma (MM) is a hematological bone marrow malignancy that can be treated but is usually fatal. Medication resistance is the major cause of relapses due to cancer stem cells (CSCs). As a result, this study aimed to identify multiple myeloma cancer stem cells (MMCSCs) in the bone marrow of twelve MM patients with pathological complete response (pCR) after chemotherapy and to investigate the potential effect of Curcumin/Piperine (C/P) extract as an anti-MMCSCs treatment in twenty newly diagnosed patients. Methods: This study included twenty bone marrow (BM) samples from newly diagnosed MM patients and twelve BM samples from pCR patients after a year of treatment. The MTT test was performed to assess the treatment’s effective dosage. A flow cytometer was used to identify MMCSCs, cell cycle profile, extract’s apoptotic activity, and proliferation marker in the selected samples. Also, a colony formation test and stemness protein were investigated. Results: In newly diagnosed MM patients, the C/P extract suppressed MMCSCs by 64.71% for CD138-/CD19- and 38.31% for CD38++. In MM patients’ samples obtained after one year of treatment, the MMCSCs inhibition percentage reached 44.71% (P < 0.008) for CD138-/CD19- and 36.94% (P < 0.221) for CD38++. According to cell cycle analyses, the number of cells treated with C/P extract was significantly reduced in the S and G0/G1 phases (87.38%: 35.15%, and 4.83%: 2.17% respectively), with a rapid increase in the G2/M phases (1.1%: 2.2%.). MMCSCs apoptosis was identified using a flow cytometer and Annexin-V. Multiple myeloma stem cell (MMCSC) proliferation was inhibited. Clonogenicity was suppressed by 60%, and stemness protein expression was reduced by 70%. Conclusion: MMCSCs in the bone marrow of MM-pCR patients can be utilized as a prognostic tool to predict recurrent multiple myeloma incidence. Also, the therapeutic potential of C/P extract as a prospective anti-MM drug targeting MMCSCs.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3507
3515
https://journal.waocp.org/article_90344_8d79692020983492e2c2d06c1960c5e3.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3507
Relapsed Childhood Acute Lymphoblastic Leukemia: Experience from a Single Tertiary Center in Thailand
Thirachit
Chotsampancharoen
Division of Hematology and Oncology, Department of Pediatrics, Prince of Songkla University, Hat Yai, Thailand.
author
Natsaruth
Songthawee
Division of Hematology and Oncology, Department of Pediatrics, Prince of Songkla University, Hat Yai, Thailand.
author
Shevachut
Chavananon
Division of Hematology and Oncology, Department of Pediatrics, Prince of Songkla University, Hat Yai, Thailand.
author
Pornpun
Sripornsawan
Division of Hematology and Oncology, Department of Pediatrics, Prince of Songkla University, Hat Yai, Thailand.
author
Edward
McNeil
Epidemiology
Unit, Faculty of Medicine, Prince of Songkla University, Hat Yai, Thailand.
author
text
article
2022
eng
Background: The outcomes of relapsed childhood acute lymphoblastic leukemia (ALL) in developed countries have improved over time as a result of risk-adapted, minimal residual disease-directed therapy, hematopoietic stem cell transplantation, and immunotherapy. There are few studies that have examined survival in relapsed childhood ALL in resource-limited countries. Therefore, this study aimed to assess the prognostic factors and survival outcome of relapsed childhood ALL in a major tertiary center in Southern Thailand. Methods: The medical records of patients with ALL aged <15 years between January 2000 and December 2019 were retrospectively reviewed. The Kaplan-Meier method was used to depict the overall survival (OS). Results: A total of 472 patients with ALL were enrolled and relapsed ALL was found in 155 (32.8%) patients. Of these, 131 (84.5%) and 24 (15.5%) had B-cell and T-cell phenotypes, respectively. One hundred thirteen (72.9%) and 42 (27.1%) patients had early and late relapses, respectively. The most common site of relapse was bone marrow in 102 patients (65.8%). One hundred twenty-eight (82.6%) patients received treatment while 27 (17.4%) patients refused treatment. The 5-year OS of all relapsed patients was 11.9%. The 5-year OS among the patients with early relapse was significantly lower than in the patients with late relapse (5.3% vs. 29.1%, respectively, p <0.0001). Site and immunophenotype were not associated with survival of relapsed ALL. The median survival times among the patients who received and refused relapse chemotherapy were 11.8 and 3.1 months, respectively (p <0.0001). Conclusion: The relapse rate accounted for one third of patients with ALL with the 5-year OS of 12%. Early relapse and those who refused treatment were associated with poor survival outcome.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3517
3522
https://journal.waocp.org/article_90332_3c764d463aff5e3466b9d3ebf503e62b.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3517
Role of EFNA1 SNP (rs12904) in Tumorigenesis and Metastasis of Colorectal Cancer: A Bioinformatic Analysis and HRM SNP Genotyping Verification
Elham
Salem
Department of Molecular Medicine and Genetics, Medical School, Hamadan University of Medical Sciences, Hamadan, Iran.
author
Amir
Keshvari
Department of Surgery, Tehran University of Medical Sciences, Tehran, Iran.
author
Ali
Mahdavinezhad
Department of Molecular Medicine and Genetics, Medical School, Hamadan University of Medical Sciences, Hamadan, Iran.
author
Ali Reza
Soltanian
Biostatistics and Epidemiology, School of Health, Hamadan University of Medical Sciences, Hamadan, Iran.
author
Massoud
Saidijam
Research Center for Molecular Medicine, Hamadan University of Medical Sciences,
Hamadan, Iran.
author
Saeid
Afshar
Department of Molecular Medicine and Genetics, Medical School, Hamadan University of Medical Sciences, Hamadan, Iran.
author
text
article
2022
eng
Objective: Colorectal cancer is a prevalent disease with a poor prognosis and is known as a heterogeneous disease with many differences in clinical Symptoms and molecular profiles. The present study aimed to systematically evaluate the association of SNPs in miRNA binding sites of target genes that are involved in CRC angiogenesis, epithelial to mesenchymal transition, and cytoskeleton organization with tumorigenesis and metastasis of CRC. Methods: A case-control study was performed on 146 samples of CRC patients and 132 healthy samples. After that, the DNA of all samples was isolated by the salting-out method. Finally, the genotypes for EFNA1 SNP (rs12904) were identified by HRM (High-resolution melting analysis) method. In order to evaluate the results of genotyping, two samples from each genotype were sequenced using the sanger sequencing method. Result: The frequency of AA genotype and the frequency of GG for rs12904 in satge4 and other stages are different from each other (P-value <0.0001) (P-value = 0.008). Also, the frequency of AA genotype in patients with different grades is different from each other (P-value = 0.035), while the frequency of AG genotype and the frequency of GG genotype is not significantly different in patients with different grades (P-value = 0.377) (P-value = 0.284). Conclusion: Results of this study indicated that patients carrying the GA and GG genotypes reduced the risk of disease progression compared to the AA genotype. As a result, this polymorphism plays a key role in CRC pathogenesis and metastasis and could be used as a biomarker in molecular diagnosis and metastatic state prediction in the near future after further study of its signaling pathways and molecular mechanism.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3523
3531
https://journal.waocp.org/article_90333_15ebdf62159c6d4e2a78505310175d0f.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3523
Anti-Proliferative Effect of Potential LSD1/CoREST Inhibitors Based on Molecular Dynamics Model for Treatment of SH-SY5Y Neuroblastoma Cancer Cell Line
Hiba
Zalloum
Hamdi Mango Research Center for Scientific Research, The University of Jordan, Amman 11942, Jordan.
author
Waleed
Zalloum
Department of Pharmacy,
Faculty of Health Science, American University of Madaba, P.O. Box 2882, Amman 11821, Jordan.
author
Tareq
Hameduh
Hamdi Mango Research Center for Scientific Research, The University of Jordan, Amman 11942, Jordan.
author
Husam
ALSalamat
Hamdi Mango Research Center for Scientific Research, The University of Jordan, Amman 11942, Jordan.
author
Malek
Zihlif
Department of Pharmacology, Faculty of Medicine, The University of Jordan, Amman
11942, Jordan.
author
text
article
2022
eng
Background: Lysine-specific demethylase is a demethylase enzyme that can remove methyl groups from histones H3K4me1/2 and H3K9me1/2. It is expressed in many cancers, where it impedes differentiation and contributes to cancer cell proliferation, cell metastasis and invasiveness, and is associated with inferior prognosis. LSD1 is associated with its corepressor protein CoREST, and utilizes tetrahydrofolate as a cofactor to accept CH2 from the demethylation process. The fact that the cofactor is best bound to the active site inspired us to explore its interactions to LSD1/CoREST enzyme complex utilizing molecular dynamics simulation, which aids designing novel and potent inhibitors. Objective: In this study we minted to identify a new potential LSD1/CoREST inhibitors and test the potency and the safety of such inhibitors against human neuroblastoma and fibroblast cells lines. Methods: We have implemented a previously derived model from the molecular dynamics simulation study and the key contacts to the active site in a subsequent structure based drug design and in-silico screening, which revealed a number of potential inhibitors toward LSD1/CoREST complex. The anti-proliferative activities of the identified compounds will be tested against neuroblastoma SH-SY5Y cancer cell line which known to highly express LSD1/CoREST complex. Results: In-silico mining on National Cancer Institute (NCI) database identified 55 promising and structurally diverse inhibitors. Applying the abovementioned molecular modeling procedure yielded four compounds of LSD1/CoREST inhibiters with IC50 < 2µM. The four lead compounds were tested against SH-SY5Y neuroblastoma cell line that known to express high level of LSD1 and illustrated a potent activity with an IC50 ranging from 0.195 to 1.52µM. To estimate the toxicity of the selective leads, they were tested against normal fibroblast cells and scored a relatively high IC50 ranging from 0.303 to ≥ 100µM. Conclusion: Our model revealed promising inhibitors that can be used in treating cancers that overexpress the LSD1 enzyme such as the SH-SY5Y neuroblastoma.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3533
3540
https://journal.waocp.org/article_90334_26329a8020b71dc756dd08080b4fe756.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3533
Identification of Malignancy in PAP Smear Samples Using the CGB3 and NOP56 Genes as Methylation Markers
Palak
Singh
Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok 10330 Thailand.
author
Nakarin
Kitkumthorn
Department of Oral Biology,
Faculty of Dentistry, Mahidol University, Bangkok 10400 Thailand.
author
Pattamawadee
Yanatatsaneejit
Human Genetics Research Group, Department of Botany,
Faculty of Science, Chulalongkorn University, Center of Excellence in Molecular Genetics of Cancer and Human Diseases,
Chulalongkorn University, Bangkok 10330, Thailand.
author
text
article
2022
eng
Background: Although various improvements have been made in the reporting of the Papanicolaou (PAP) test in recent years, there remain several challenges that have yet to be addressed in terms of determining a standardized methodology for categorizing atypical squamous cells of undetermined significance (ASC US). Methods: The present study focuses on evaluating the performance of the methylation status of two genes (CGB3 and NOP56) using a total of 200 PAP samples, which were divided into the “determined” group, with 78 samples based on cytology, and the “undetermined” group (ASC US), with 122 samples. The promoter methylation status of the CGB3 and NOP56 genes was detected for the 200 PAP samples using methylation specific PCR (MSP). The diagnostic abilities of the CGB3 and NOP56 genes in PAP samples were measured, and receiver operating characteristic (ROC) curves were generated using Python programming language. Results: Based on the validation of CGB3 and NOP56 methylation in the 200 PAP samples, both genes exhibited higher methylation percentages in abnormal samples compared with normal samples. In addition, on the basis of diagnostic performance analysis, the CGB3 gene exhibited the highest sensitivity and specificity in both histology based ASC US and cytology based ‘determined’ PAP samples, with significant diagnostic abilities [area under the curve (AUC) values of 0.83 and 0.74, respectively, where AUC ≥0.5 was determined to be significant] to distinguish between the “normal” and “abnormal” samples. Conclusion: The findings of the present study will contribute toward identifying a DNA methylation marker for the early detection of abnormal samples before they reach the initial stages of cervical cancer, and should prove to be helpful for clinicians in terms of diagnosing patients whose cells are ASC US.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3541
3551
https://journal.waocp.org/article_90337_5603dcd352e70ce6e76095ee78737065.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3541
SNP rs9387478 at ROS1-DCBLD1 Locus is Significantly Associated with Lung Cancer Risk and Poor Survival in Indian Population
Jonita
Chongtham
Department of Genetics, University of Delhi South Campus, New Delhi, India.
author
Namita
Pandey
Department of Genetics, University of Delhi South Campus, New Delhi, India.
author
Lokesh
Sharma
Ram Manohar Lohia Hospital, New Delhi, India.
author
Anant
Mohan
Department of Pulmonary,
Critical Care and Sleep Medicine, All India Institute of Medical Sciences (AIIMS), New Delhi, India.
author
Tapasya
Srivastava
Department of Genetics, University of Delhi South Campus, New Delhi, India.
author
text
article
2022
eng
Objective: Receptor tyrosine kinases (RTK) are relevant therapeutic targets in the treatment of lung cancer. Germline susceptibility variants that influence these RTKs may provide new insights into their regulation. rs9387478 is located in the genomic interval between two RTK-genes ROS1/DCBLD1, of which ROS1 alterations are implicated in lung carcinogenesis and treatment response while the latter remains poorly understood. Materials and methods: Venous blood was drawn from 100 control and 231 case subjects. Genotype was scored by restriction fragment length polymorphism (RFLP), PCR amplification followed by HindIII digestion. Logistic regression was applied to compare the association between variables. Survival curve was plotted to draw a correlation between the genotype and overall survival. Also, eQTL and chromatin state changes were analyzed and correlated with the survival of patients using available datasets. Results: In our population smoking correlated significantly with lung cancer [OR= 2.607] with the presence of the minor allele ‘A’ enhancing the nicotine dependence [CA (OR=3.23)]. Individuals with homozygous risk allele ‘A’ had a higher chance of developing lung cancer [OR=2.65] than individuals with CA/CC implying a recessive model of association. Patients with CC/CA genotype had better overall survival than patients with AA genotype [161 days/142 days vs 54 days, p=0.005]. The homozygous risk allele was significantly associated with increased DCBLD1 and ROS1 expression in lung cancer, with enriched active histone marks due to the polymorphism. Interestingly, increased DCBLD1 expression was associated with poor outcomes in lung cancer. Conclusion: Overall, our study provides strong evidence that rs9387478 is significantly associated with both nicotine dependence and lung cancer in our North Indian cohort. The association of the SNP with prognostic genes, DCBLD1 and ROS1 make rs9387478 a promising prognostic marker in the North Indian population. The results obtained are significant, however, the study needs to be performed in a larger sample size.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3553
3561
https://journal.waocp.org/article_90345_8b790716810e7d4a654f7fe80e73873d.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3553
Phycosynthesis of Silver Nanoparticles Using Cladophora Glomerata and Evaluation of Their Ability to Inhibit the Proliferation of MCF-7 and L20B Cell Lines
Raghad
Fayyad
Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq.
author
Alaa
Mohammed Ali
Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq.
author
Noor Al-Huda
Saeed
Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq.
author
Israa
Hamzah
Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq.
author
Ahmed
Dwaish
Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq.
author
text
article
2022
eng
Background: Nanotechnology is receiving greater attention these days as a result of its applications in numerous industrial, medical, and environmental fields. Objective: To synthesize silver nanoparticles with a green alga, Cladophora glomerata, and determine their inhibitory activity against tumor cell (MCF-7) and transgenic mouse cell (L20B) lines. Materials and Methods: Methanol extract was prepared from Cladophora glomerata and used as a safe factory for the synthesis of silver nanoparticles (AgNPs). UV-visible spectrophotometer, X-ray diffraction, scanning electron microscopy, and EDX analyses were used to characterize the biosynthesized AgNPs. The anti-tumor activity of the phycosynthesized AgNPs was tested against the MCF-7 and L20B cell lines. Furthermore, the bioactive compounds in the algal extract were determined by gas chromatography-mass spectroscopy (GC-MS). Results: The phycosynthesis produced clusters of spherical and polydispersed cuboidal pure AgNPs with an average size of 32 nm. The phycosynthesized AgNPs possess anti-cancer and anti-tumor activities on the MCF-7 and L20B cell lines, with significant anti-proliferation percentages of 52.8 and 65.8%, respectively, after 48 hours of treatment with 100 μg/ml AgNPs. Both treated cell lines showed a significant change in cellular shape and tissue detachment. The GC-MS analysis revealed the presence of a high proportion of octadecanoic acid (47.59%) and hexadecanoic acid (14.97%). Conclusion: Cladophora glomerata contains chemicals that improve the stabilization and reduction properties of the nanoparticles. It can be used as a safe, local, and natural source for the synthesis of AgNPs and can also be used as a benign factory for many other metal nanoparticles. The phycosynthesized AgNPs have anti-cancer and anti-tumor activities on the test cell lines and provide an insight into the potential for using them as a trend in cancer nanotherapy.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3563
3569
https://journal.waocp.org/article_90335_33662971ed5c09ef7657d8c3d7f2012a.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3563
The Correlation Between CD44 and Angiogenesis in Oral Squamous Cell Carcinoma Induced in Buccal Pouch in Syrian Hamster that Underwent Radiotherapy
Reham
Alsafadi
Department of Oral Pathology, Faculty of Dentistry, Damascus University, Syria.
author
Ahmad
Manadili
Department of Oral Pathology, Faculty of Dentistry, Damascus University, Syria.
author
text
article
2022
eng
Background: Angiogenesis with radiotherapy is a significant focus of recent studies to confirm the importance of combined treatments, as vascular control can have a great therapeutic target. Vascular endothelial growth factor is the key mediator of angiogenesis in cancer. In addition, some studies suggest the value of CD44 as a potential early marker of angiogenesis. Objectives: Investigating the expression of vascular endothelial growth factor (VEGF) and CD44 in oral squamous cell carcinoma (OSCC) after inducing it in hamsters then undergoing radiotherapy and comparing outcomes before and after therapy to verify changes of these markers. Materials & methods: an experimental study consisted of 18 samples of OSCC which induced in right buccal pouch of hamsters (group1) and 18 samples of OSCC which induced in the same way and were exposed to radiation therapy (group2), Biopsies were taken and fixed with formalin, paraffin waxed in conventional H&E and immunostained with monoclonal anti-VEGF and anti-CD44. Results: our findings didn’t reveal a statistically significant difference in the expression of VEGF(p =0.342) and CD44 (p=0.187) between group1 & group 2. moreover, we found tumor cells which weren’t affected and resistant to radiotherapy, also revealed positive expression of VEGF & CD44, otherwise, we noticed Pearson coefficient was a significant correlation that indicated to a moderate relation. Conclusion: cancerous cells that showed a high expression of these markers, give elevated radiosensitivity and resist the treatment. Subsequently, we assure the importance of applying anti-VEGF and/or anti-CD44 as a supportive therapy with radiation therapy.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3571
3576
https://journal.waocp.org/article_90339_b2fc238389e60645c4b2b80f3146b411.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3571
DNA Repair Genes Polymorphisms: Impact on Acute Myeloid Leukemia Patients Outcome
Salah
Aref
Hematology Unit, Clinical Pathology Department, Faculty of Medicine, Mansoura University, Egypt.
author
Nadia
El Menshawy
Hematology Unit, Clinical Pathology Department, Faculty of Medicine, Mansoura University, Egypt.
author
Tarek
Abou Zeid
Unit,
Mansoura University Oncology Center (MUOC), Mansoura University, Egypt.
author
Enas
Gouda
Hematology Unit, Clinical Pathology Department, Faculty of Medicine, Mansoura University, Egypt.
author
Nora
Abdel Aziz
Hematology Unit, Clinical Pathology Department, Faculty of Medicine, Mansoura University, Egypt.
author
text
article
2022
eng
Background: ATM; XRCC6 and LIG4 genes play an important role in repairing the double-strand DNA breaks and maintaining the genome stability. Single nucleotide polymorphisms (SNPs) in these genes could affect these genes expression and function. The aim of this study was to address the effect of SNP of the DNA repairing genes on corresponding gene expression as well as AML patient’s outcome. Subjects and Methods: This is cross sectional study included 95 newly diagnosed AML patients. For all subjects included in our study SNPs and expression of ATM (rs189037G>A), XRCC6 (rs2267437C>G) and LIG4 (rs1805388C>T) genes were evaluated by RFLP and real time PCR. Results:The following SNPs in ATM (AA); XRCC6 (GG); and LIG4 (TT) are associated with down regulation of the corresponding genes (P<0.001). The lower expression of ATM and LIG4 genes are associated with shorter OS and DFS. Cox regression multivariate analysis revealed that lower expression of ATM HR : 2.02 (CI: 1.12-3.64; p=0.020. Conclusion: The following SNPs of ATM (AA); XRCC6 (GG); and LIG4 (TT) are associated with down regulation of corresponding genes expression. ATM and XRCC6 lower expression are predictors of OS while ATM is predictor of DFS and could be used for optimizing the AML therapy.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3577
3585
https://journal.waocp.org/article_90346_18422bc9e9816258788dcd0a54b195cd.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3577
Nurses’ Perceived Challenges in the Management of Hospitalized Cancer Patients in a Comprehensive Cancer Center in Southeastern Nigeria
Peace
Iheanacho
Department of Nursing Sciences, Faculty of Health Science and Technology, University of Nigeria, Enugu Campus, Nigeria.
author
Hope
Opara
Department of Nursing Sciences, Faculty of Health Science and Technology, University of Nigeria, Enugu Campus, Nigeria.
author
Juliana
Ifezulumba
Department of Nursing Sciences, Faculty of Health Science and Technology, University of Nigeria, Enugu Campus, Nigeria.
author
Ngozi
Omotola
Department of Nursing Sciences, Faculty of Health Science and Technology, University of Nigeria, Enugu Campus, Nigeria.
author
Justin
Ingwu
Department of Nursing Sciences, Faculty of Health Science and Technology, University of Nigeria, Enugu Campus, Nigeria.
author
Agnes
Anarado
Department of Nursing Sciences, Faculty of Health Science and Technology, University of Nigeria, Enugu Campus, Nigeria.
author
text
article
2022
eng
Objective: Nurses’ challenges in poor-resource countries like Nigeria have been understudied. This study determined nurses’ perceived challenges in management of hospitalized cancer patients in a comprehensive cancer center in southeast of Nigeria. Methods: The descriptive study included 133 registered nurses working in medical-surgical and oncology wards. A 37-item questionnaire included seven (7) questions on socioeconomic, 16 questions related to nurse’s knowledge, and 14 questions related to cancer treatment and the hospital facility. A significant challenge was defined as a mean score of more than 3 in a Likert scale 5 points. Results: Challenges included a shortage of nurses (inadequate numbers of skilled nurses in oncology and other wards with mean±sd score (4.73±0.58), lack of continuing education on current trends in the management of cancer (4.03±0.45), and lack of oncology trained nursing experts for job mentoring (4.24±0.77). Others were managing patients facing chemotherapy related side-effects (3.06±2.12), high cost of treatment borne by cancer patients (4.41±0.68) and exorbitant hospital bills (4.72±0.48), non-availability of drugs (4.09±0.87) and institutional policy bottlenecks affecting subsidizing treatment costs (4.09±0.84). Cancer care affected mainly by inadequate and functional equipment (4.24±0.55), and with no staff remuneration (4.53± 0.85). Conclusion: Nurses’ Perceived Challenges were related to professional, institutional, and those related to patient.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3587
3593
https://journal.waocp.org/article_90347_94ce11d8d867429c8011e119f8a0e1ec.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3587
Association between Serum Progastrin Biomarker Level and Gastric Cancer
Omolbanin
Amjadi
Gastrointestinal Cancer Research Center, Non-communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran.
author
Reza
Alizadeh-Navaei
Gastrointestinal Cancer Research Center, Non-communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran.
author
Mahsa
Rezapour
Gastrointestinal Cancer Research Center, Non-communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran.
author
Versa
Omrani-Nava
Gastrointestinal Cancer Research Center, Non-communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran.
author
Mahmood
Moosazadeh
Gastrointestinal Cancer Research Center, Non-communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran.
author
Hossein
Azadeh
Department of Internal Medicine, Rheumatology Division, Mazandaran University of Medical Sciences, Sari, Iran.
author
Ehsan
Zaboli
Gastrointestinal Cancer Research Center, Non-communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran.
author
Mohadeseh
Ahmadi
Gastrointestinal Cancer Research Center, Non-communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran.
author
Akbar
Hedayatizadeh-Omran
Gastrointestinal Cancer Research Center, Non-communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran.
author
text
article
2022
eng
Background and Objective: gastric cancer is the fifth most prevalent cancer and the fourth cause of death because of cancer. In Iran, northern and northwestern regions are considered gastric cancer hot spots. Identifying serum biomarkers could be helpful in early diagnosis of patients with gastric adenocarcinoma (GAC). Increase in progastrin level has been reported in different cancers. Given the diagnostic value of this biomarker, this study aimed to determine the diagnostic role of progastrin serum biomarker in patients with gastric cancer. Methodology: In this case-control study, forty patients with gastric cancer who were diagnosed by endoscopy and pathologic findings and visited Mazandaran Comprehensive Cancer Center. The participants had received no treatment yet and entered this study. The participants in case group were compared with the control group including forty-two individuals with no history of gastrointestinal cancer in their first-degree relatives and visiting the lab for routine tests. Progastrin serum level was assessed using ELISA kit. The Kruskal-Wallis test and Mann Whitney test, both non-parametric) were used for statistical analysis and the relation between the variables was examined using Pearson’s correlation coefficient at 95% confidence level in SPSS 16. Findings: In this study, progastrin serum level was significantly higher in patients with gastric cancer compared with normal participants (P = 0.035). Progastrin serum level had no significant relation with tumor clinicopathologic parameters (p-value > 0.05). Conclusion: Increase in progastrin may be utilized as a predictive factor for gastric cancer.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
23
v.
10
no.
2022
3595
3599
https://journal.waocp.org/article_90348_4ce4df8b0b96b1473fc630d0489234a1.pdf
dx.doi.org/10.31557/APJCP.2022.23.10.3595