UICC-ARO Symposium at the UICC 2016 World Cancer Congress How Can We Mobilize Action to Realize UHC in Asia?
Hideyuki
Akaza
Department of Strategic Investigation on Comprehensive Cancer Network, Research Center for Advanced Science and Technology
(RCAST), the University of Tokyo, UICC-Asia Regional Office (UICC-ARO), Japan.
author
Norie
Kawahara
Department of Strategic Investigation on Comprehensive Cancer Network, Research Center for Advanced Science and Technology
(RCAST), the University of Tokyo, UICC-Asia Regional Office (UICC-ARO), Japan.
author
Takashi
Fukuda
National Institute of Public Health, Japan.
author
Shigeo
Horie
Juntendo University Graduate School of Medicine, Japan.
author
Hasbullah
Thabrany
Universita Indonesia, Indonesia.
author
Shinjiro
Nozaki
WHO Kobe Center, Japan.
author
text
article
2017
eng
The 2016 World Cancer Congress, organised by UICC, was held in Paris in November 2016, under the theme "Mobilizing action – Inspiring Change." As part of Track 4 presentations on the theme of "Strengthening cancer control: optimizing outcomes of health systems," UICC-Asian Regional Office (UICC-ARO) held a symposium to discuss the issue of mobilizing action to realize UHC in Asia. Introducing the symposium, Hideyuki Akaza noted that universal health coverage (UHC) is included in the Sustainable Development Goals and one of the key issues for achieving UHC will be how to balance patient needs with the economic burden of cancer. Speakers from Japan and Indonesia addressed various issues, including the current status and challenges for medical economic evaluation in Asia, the importance of resource stratification, prospects for precision medicine, and the outlook for cancer control and UHC in developing and emerging countries in Asia. Key issues raised included how to respond to the rising costs of treating cancer as new and increasingly expensive drugs come to the market. Speakers and participants noted that health technology assessment programs are being developed around Asia in order to evaluate the cost-effectiveness of drugs in the face of budgetary constraints within increasingly pressurized national health systems. The importance of screening and early detection was also noted as effective means that have the potential to reduce reliance on expensive drugs for advanced cancers. The symposium was chaired jointly by Hideyuki Akaza and Shinjiro Nozaki (WHO Kobe Centre).
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
2897
2901
https://journal.waocp.org/article_52163_d443703bf3b053b606b3551b796542f2.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.2897
Clinicopathological Significance of MTA 1 Expression in Patients with Non-Small Cell Lung Cancer: A Meta-Analysis
Wei
Zhu
Department of Pathology, School of Basic Medicine, Guangdong Medical University, Dongguan, Guangdong Province, China.
author
Guixian
Li
Department of Pathology, School of Basic Medicine, Guangdong Medical University, Dongguan, Guangdong Province, China.
author
Haina
Guo
Department of Pathology, the Affiliated Donghua Hospital of Sun Yat-Sen University, Dongguan, Guangdong Province, China.
author
Honglong
Chen
Department of Phamacology, Guangdong Medical University, Dongguan, Guangdong Province, China.
author
Xiujuan
Xu
Department of Epidemiology and Biostatistics, School of Public
Health, Guangdong Medical University, Dongguan, Guangdong Province, China.
author
Jiali
Long
Department of Pathology, School of Basic Medicine, Guangdong Medical University, Dongguan, Guangdong Province, China.
author
Chao
Zeng
Department of Pathology, School of Basic Medicine, Guangdong Medical University, Dongguan, Guangdong Province, China.
author
Xiaojun
Wang
Department of Epidemiology and Biostatistics, School of Public
Health, Guangdong Medical University, Dongguan, Guangdong Province, China.
author
text
article
2017
eng
Background: Metastasis associated gene 1(MTA1) is one of the most deregulated molecules in human cancer and leads to cancer progression and metastasis. We performed a meta-analysis to determine the correlations between MTA1 expression and the clinicopathological characteristics of non-small cell lung cancer (NSCLC). Methods: We searched PubMed, Springer, Science Direct, Google Scholar and China National Knowledge Infrastructure (CNKI) for relevant articles. For statistical analyses, we used R3.1.1 software. The fixed or random effects model was employed based on the results of the statistical test for homogeneity. Results: Seven studies involving 660 NSCLC patients were included. The proportion of MTA1 overexpression with 95% confidence interval (95% CI) was 0.53(95%CI: 0.43-0.62) in NSCLC patients; 0.47(95%CI: 0.40-0.55) in age ) and 0.57(95%CI: 0.46-0.67) in adenocarcinoma (AC); 0.39(95%CI: 0.23-0.56) in well-differentiated tumors, 0.44(95%CI: 0.37-0.51) in moderately differentiated tumors and 0.55(95%CI: 0.37-0.51) in poorly differentiated tumors; 0.48(95%CI: 0.36-0.60) in clinical grade (III-IV) NSCLC and 0.75 (95%CI: 0.69-0.81) in clinical grade (I-II) NSCLC; 0.58(95%CI: 0.45-0.71) in T Stage (T1/T2) NSCLC; 0.68(95%CI: 0.49-0.82) in NSCLC patients with lymph node positivity and 0.51(95%CI: 0.43-0.58) in NSCLC patients with lymph node negativity. Conclusions: These results indicated that MTA1 might be a valuable biomarker in the diagnosis of NSCLC. MTA1 overexpression was significantly associated with age ≥60 years, gender, histopathological type, clinical grade (I-II), T stage (T1/T2) and lymph node positivity in NSCLC patients.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
2903
2909
https://journal.waocp.org/article_51943_a1088df3c69b3d9f8afd20d2896a762c.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.2903
Dual Function of Gold Nanoparticles in Synergism with Mitoxantrone and Microwave Hyperthermia Against Melanoma Cells
Ahmad
Shanei
Department of Medical Physics and Medical Engineering and Student Research Committe, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
author
Ameneh
Sazgarnia
Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
author
Elham
Dolat
Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
author
Leila
Hojaji-Najafabadi
Nanotechnology Department, Faculty of Advanced Sciences and Technologies, Isfahan University, Isfahan, Iran.
author
Mohammadreza
Sehhati
Department of Bioelectric and Biomedical Engineering, School of Advanced Technologies in Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
author
Milad
Baradaran-Ghahfarokhi
Department of Medical Physics and Medical Engineering and Student Research Committe, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
author
text
article
2017
eng
Background: This study was performed to evaluate any synergetic effects of mitoxantrone (MX) and gold nanoparticles (GNPs) as dual therapeutic approach, along with microwave (MW) hyperthermia for melanoma cancer. Methods: Various tests were performed on the DFW melanoma cell line in the presence of MX and different concentrations of GNPs, with and without MW irradiation. MTT [3-(4,5-dimethylthiazol–2-yl)-2,5-iphenyltetrazolium bromide] assays were conducted to evaluate the effectiveness of the used therapeutic methods in terms of cell survival. Relative lethal synergism (RLS) was calculated as the ratio of cell death following hyperthermia in the presence of a treatment agent to that after applying hyperthermia in the absence of the same treatment agent. Results: Results showed MX and GNPs under MW irradiation to provide maximum cell death (P < 0.001 compared to the other groups). The mean RLS for MW hyperthermia along with the MX-GNP combination was 4.14, whereas in the absence of GNP the value for MX chemotherapy was 0.94. Conclusion: MX chemotherapy in the presence of different concentrations of GNP did not alter cell survival as compared to in its absence.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
2911
2917
https://journal.waocp.org/article_51932_8dc1e7b2c317097b56104acd485360b1.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.2911
Anthocyanins from the Fruit of Vitis Coignetiae Pulliat Inhibit TNF-Augmented Cancer Proliferation, Migration, and Invasion in A549 Cells
Jing Nan
Lu
Department of Internal Medicine, Institute of Health Science, Gyeongsang National University School of Medicine, Jinju, 660-702 Korea.
author
Radha
Panchanathan
Department of Internal Medicine, Institute of Health Science, Gyeongsang National University School of Medicine, Jinju, 660-702 Korea.
author
Won Sup
Lee
Department of Internal Medicine, Institute of Health Science, Gyeongsang National University School of Medicine, Jinju, 660-702 Korea.
author
Hye Jung
Kim
Pharmacology, Institute of Health Science, Gyeongsang National University School of Medicine, Jinju, 660-702 Korea.
author
Dong Hoon
kim
Emergency Medicine, Institute of Health Science, Gyeongsang National University School of Medicine, Jinju, 660-702 Korea.
author
Yung Hyun
Choi
Department of Biochemistry, Dongeui University College of Oriental
Medicine and Department of Biomaterial Control (BK21 program), Dongeui University Graduate School, Busan 614-052, Korea.
author
GonSup
Kim
School
of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University, Jinju, 660-
701, Korea.
author
Sung Chul
Shin
Department of Chemistry, Research Institute of Life Science, Gyeongsang National University, Jinju, 660-701, Korea.
author
Soon Chan
Hong
Surgery Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju, 660-702, Korea.
author
text
article
2017
eng
Objective: Anthocyanins belong to a class of flavonoids, exhibiting antioxidant and anti-inflammatory actions have been reported to have anti-cancer effects. Here, we investigated whether anthocyanins can inhibit cancer cell proliferation, invasion, and angiogenesis in human lung cancer A549 cells, which are critically involved in cancer metastasis. Methods: We used anthocyanins from fruits of Vitis coignetiae Pulliat (AIMs) which has been used in Korean folk medicine for the treatment of inflammatory diseases and cancers. We have performed cell proliferation assays, cell invasion assay, gelatin zymography, wound healing assay and western blotting to examine whether anthocyanins can inhibit cancer cell proliferation, invasion, and angiogenesis in A549 cells. Result: AIMs did not inhibit cancer cell proliferation on A549 cells. Also, AIMs suppressed cancer migration, and invasion by supressing MMP-2 and MMP-9 expression. The Immuno-blotting results also revealed that AIMs suppressed the proteins involved in cancer proliferation (COX- 2, C-myc, cyclin D1), migration and invasion (MMP-2, MMP-9), anti-apoptosis (XIAP, and c-IAP2), adhesion and angiogenesis (ICAM-1, VEGF). Conclusion: This study demonstrates that the anthocyanins isolated from fruits of Vitis coignetiae Pulliat inhibit cancer proliferation, cancer migration, and invasion that is involve in cancer-metastasis. This study provides evidence that AIMs might have anti-cancer effects on human lung cancer.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
2919
2923
https://journal.waocp.org/article_51769_890bf8c451319e3ddef70eb3a44076c2.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.2919
The Lys469glu/K469E Polymorphism of the Inflammatory Gene Intercellular Adhesion Molecule-1 Lacks any Apparent Role in the Polycystic Ovary Syndrome in Kashmiri Women: A Case Control Study
Syed Douhath
Yousuf
Department of Biochemistry, University of Kashmir, J and K, India.
author
Mohammad Ashraf
Ganie
Sher-i- kashmir institute of medical sciences srinagar ,Srinagar, J and K, India.
author
Mohammad Afzal
Zargar
Department of Biochemistry, University of Kashmir, J and K, India.
author
shajr Ul
Amin
Department of Biochemistry, University of Kashmir, J and K, India.
author
Gulzar
Bhat A
Department of Biochemistry, University of Kashmir, J and K, India.
author
Akbar
Masood
Department of Biochemistry, University of Kashmir, J and K, India.
author
Fouzia
Rashid
Department of Biochemistry, University of Kashmir, J and K, India.
author
text
article
2017
eng
Background: Polycystic ovary syndrome (PCOS), associated with a state of low grade chronic inflammation, depends on multiple genetic and environmental factors. Elevated levels of inflammatory markers including intercellular adhesion molecule-1 (ICAM-1) have been demonstrated in affected women. Recent evidence indicates a significant linkage between chromosome 19p13 loci and multifactorial diseases that have an inflammatory component. The aim of this study was to assess the possible association of the lys469glu (K469E) polymorphism of the ICAM-1 gene located on chromosome 19p13 with risk of PCOS in Kashmiri women. Material and Methods: The K469E single nucleotide polymorphism (SNP) was analysed with DNA from peripheral blood leukocytes of 220 PCOS cases and 220 age matched non-PCOS healthy controls using PCR-RFLP. Results: Genotypic frequencies in cases were found to be 32 (14.5%) for EE, 98 (44.5%) for KE, and 90 (40.9%) for KK, with 130 (59.1%) for the KE+EE genotypes compared to healthy control values of 29 (13.2%) for EE, 113 (51.4%) for KE, 78 (35. 5%) for KK and 142 (64.5%) for KE+EE combined.The odds ratios for the EE, KE and KE:EE genotypes were 0.95(95% CI= 0.53-1.71)[p= 0.88], 0.75(95% CI= 0.50-1.12)[p =0.168] and 0.79 (95% CI =0.53-1.16) [p = 0.23], no statistically significant differences being found between cases and controls (χ2 =2.07; p=0.35). Conclusion: In conclusion, there was no apparent significant influence of the K469E polymorphism on risk of PCOS, or any clinical or laboratory parameters.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
2925
2930
https://journal.waocp.org/article_51461_0743e3074998384352691bdbdd0b1326.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.2925
Knowledge of Evidence-Based Cancer Risk Factors Remains Low Among New Zealand Adults: Findings from Two Cross-Sectional Studies, 2001 and 2015
Rosalina
Richards
Cancer Society Social and Behavioural Research Unit, Department of Preventive and Social Medicine, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand.
author
Bronwen
McNoe
Cancer Society Social and Behavioural Research Unit, Department of Preventive and Social Medicine, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand.
author
Ella
Iosua
Department of Preventive and Social Medicine, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand.
author
Anthony I
Reeder
Cancer Society Social and Behavioural Research Unit, Department of Preventive and Social Medicine, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand.
author
Richard
Egan
Cancer Society Social and Behavioural Research Unit, Department of Preventive and Social Medicine, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand.
author
Louise
Marsh
Cancer Society Social and Behavioural Research Unit, Department of Preventive and Social Medicine, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand.
author
Lindsay
Robertson
Cancer Society Social and Behavioural Research Unit, Department of Preventive and Social Medicine, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand.
author
Brett
Maclennan
Cancer Society Social and Behavioural Research Unit, Department of Preventive and Social Medicine, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand.
author
Anna TF
Latu
Kōhatu – Centre for Hauora Māori, Division of Health Sciences, University of Otago, Dunedin, New Zealand.
author
Robin
Quigg
Cancer Society Social and Behavioural Research Unit, Department of Preventive and Social Medicine, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand.
author
Anne-Cathrine
Petersen
Cancer Society Social and Behavioural Research Unit, Department of Preventive and Social Medicine, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand.
author
text
article
2017
eng
Objective: Cancer risk reduction messages are a part of cancer control efforts around the world. The complex reality is that risk factors differ for different types of cancer, making clear communication of desired behavioural changes more difficult. This study aims to describe awareness of risk factors for breast, bowel, cervical, prostate and lung cancer and cutaneous melanoma among New Zealanders in 2014/15 and identify changes in awareness since 2001. Methods: Two national telephone surveys, the first (CAANZ01) conducted in 2001, included 438 adults (231 females and 207 males, 64% response rate). The second, conducted in 2014/15 (CAANZ15), included 1064 adults (588 females and 476 males, 64% response rate). Results: In 2014/5, most participants could identify evidence-based risk factors for lung cancer and melanoma. In contrast, many participants were unable to name any risk factors (evidence-based or otherwise) for bowel (34.8%), breast (48.8%), cervical (53.9%) and prostate cancer (60.9%). Between 2001 and 2014/5 there were increases in the proportion of individuals identifying sunbeds as increasing melanoma risk, and alcohol consumption and family history as increasing risk for bowel and breast cancer. Conclusions: Effective communication of risk information for specific cancers remains a challenge for cancer control. Although some positive changes in awareness over the 14 year period were observed, there remains substantial room for progressing awareness of evidence-based risk factors.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
2931
2936
https://journal.waocp.org/article_51936_95c39ed06bed2a1dfa838f78d80b7dd2.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.2931
Modulatory Effects of Chemoradiation on Angiogenic Factors and Laminin in Cervical Cancer: Link with Treatment Response
Manoj
Sharma
Department of Radiotherapy and Oncology, Maulana Azad Medical College and Lok Nayak Hospital, New Delhi, India.
author
Rehan
Khan
Department of Biochemistry,
All India Institute of Medical Sciences, New Delhi, India.
author
Mayank
Aggarwal
Department of Radiotherapy and Oncology, Maulana Azad Medical College and Lok Nayak Hospital, New Delhi, India.
author
Alpana
Sharma
Department of Biochemistry,
All India Institute of Medical Sciences, New Delhi, India.
author
text
article
2017
eng
Objective: Carcinoma of the uterine cervix is either the first or second most common malignancy in Indian women, depending on the registry. Tumor growth and metastasis primarily are determined by angiogenesis and parameters of the molecular environment including extracellular matrix elements, growth factors and cytokines. Effects of chemo-irradiation on biomarkers like vascular endothelial growth factor (VEGF), angiopoietin-2 (Ang-2) and laminin in patients with carcinoma cervix therefore need to be explored. Methods: Circulatory and mRNA levels of VEGF, Ang-2 and laminin in patients with stage III carcinoma cervix (n=40) were compared with those of normal healthy women (n=20). Measurement was prior to treatment, and after chemotherapy and teleradiation, using high sensitivity ELISA kits and Q-PCR. Clinical response was evaluated as per WHO criteria and was assessed for correlation with the biochemical markers. Results: Levels of all the studied molecules were significantly (p<0.001) higher in patients than in controls. After treatment significant decline (p<0.001) was noted. Out of 40 patients, 33 were complete responders and 7 were non-responders on clinical assessment. On comparison of before and after treatment levels of these molecules complete responders showed significant decline whereas non-responders showed non-significant decrease. Follow-up of the responders for 3 years, revealed 28 of 33 patients to still be disease free, the other 5 demonstrating recurrence. Conclusions: Higher levels of angiogenic factors along with laminin indicate roles played in disease progression aiding angiogenesis. These markers may serve as useful tools in post treatment disease mapping, for which available imaging methods may not provide a true picture.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
2937
2944
https://journal.waocp.org/article_51778_1b24d0a185582aca995e3c2a301c4a56.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.2937
Predicting Intention Perform Breast Self-Examination: Application of the Theory of Reasoned Action
Triana Kesuma
Dewi
Department of Clinical Psychology and Mental Health, Faculty of Psychology, Universitas Airlangga, Surabaya, Indonesia.
author
Rizqy Amelia
Zein
Department of Personality and Social PsychologyFaculty of Psychology, Universitas Airlangga, Surabaya, Indonesia.
author
text
article
2017
eng
Objective: The present study aimed to examine the applicability of the theory of reasoned action to explain intention to perform breast self-examination (BSE). Methods: A questionnaire was constructed to collect data. The hypothesis was tested in two steps. First, to assess the strength of the correlation among the constructs of theory of reasoned action (TRA), Pearson’s product moment correlations were applied. Second, multivariate relationships among the constructs were examined by performing hierarchical multiple linear regression analysis. Result: The findings supported the TRA model, explaining 45.8% of the variance in the students’ BSE intention, which was significantly correlated with attitude (r = 0.609, p = 0.000) and subjective norms (r = 0.420, p =0 .000). Conclusion: TRA could be a suitable model to predict BSE intentions . Participants who believed that doing BSE regularly is beneficial for early diagnosis of breast cancer and also believed that their significant referents think that doing BSE would significantly detect breast cancer earlier, were more likely to intend to perform BSE regularly. Therefore, the research findings supported the conclusion that promoting the importance of BSE at the community/social level would enhance individuals to perform BSE routinely.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
2945
2952
https://journal.waocp.org/article_51456_226bd4ac41252aa0ba82706695080605.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.2945
Different Sources of Dignity-Related Distress in Women Receiving Chemotherapy for Breast Cancer
Somaye
Shahhoseini
Nursing and Midwifery School, Shahid Beheshti University of Medical Science, Tehran, Iran.
author
Fariba
Borhani
Medical Ethics and Law Research Center, Medical Surgical Department, Nursing and Midwifery School, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
author
Foroozan
Atashzadeh Shoorideh
Department of Nursing Management, Nursing and Midwifery School, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
author
Amir
Kavousi
School of Health, Safety and Environment Shahid Beheshti University of Medical Sciences, Tehran, Iran.
author
Hossein
Bagheri
School of Nursing and Midwifery, Shahroud University of Medical Sciences,
Shahroud, Iran.
author
Amir
Almasi-Hashiani
Department of Epidemiology and Reproductive Health, Reproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
author
text
article
2017
eng
Background: Identification of different sources of dignity-related distress experienced by people nearing the end of life may help nurses to provide better care services. This study was conducted to determine sources of dignity-related distress from the perspective of women with breast cancer undergoing chemotherapy. Materials and Methods: In this cross sectional study, the participants comprised 207 women with breast cancer undergoing chemotherapy in chemotherapy clinics in hospitals of Tehran, Iran. The Cronbach’s coefficient alpha for the PDI was 0.76. Validity of PDI by confirmatory factor analysis shows that the comparative Fit Index of this instrument is 0.96 and so it is appropriate for application in different setting. Data were analyzed by Stata version 13. Results: Patients were mostly concerned about the distress caused by disease symptoms (mean; 2.4061, S.D.; 0.96), followed by existential distress (mean; 1.8784, S.D.; 0.75), peace of mind (mean; 1.871, S.D.; 0.77), dependence (mean; 1.8647, S.D.; 0.98), and social support (mean; 1.4097, S.D.; 0.99), respectively, in order of highest scores. Conclusion: Considering that the patients were mostly concerned about the distress caused by disease symptoms, followed by existential distress, peace of mind, dependency, and social support, it seems necessary to take further measures toward addressing these issues.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
2953
2958
https://journal.waocp.org/article_51798_e5261aa32d78304cb99d4feda3fb9f9c.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.2953
SNPs in the Insulin-Like Growth Factor Gene and Obesity Impact on Colorectal Cancer in Egyptians
Ayman
Yosry
Department of Endemic Medicine and Hepatogastroenterology, Faculty of Medicine, Cairo University, Egypt.
author
Dalia
Omran
Department of Endemic Medicine and Hepatogastroenterology, Faculty of Medicine, Cairo University, Egypt.
author
Mohamad
Yousef
Department of Endemic Medicine and Hepatogastroenterology, Faculty of Medicine, Cairo University, Egypt.
author
Mohamad
Salah
Department of Endemic Medicine and Hepatogastroenterology, Faculty of Medicine, Cairo University, Egypt.
author
Heba
Omar
Department of Endemic Medicine and Hepatogastroenterology, Faculty of Medicine, Cairo University, Egypt.
author
Sherif
Hamdy
Department of Endemic Medicine and Hepatogastroenterology, Faculty of Medicine, Cairo University, Egypt.
author
Olfat
Shaker
Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Egypt.
author
Yahya
Elsherif
Elmanial Specialized Hospital, Cairo University, Egypt.
author
Mohamad S
Marie
Department of Endemic Medicine and Hepatogastroenterology, Faculty of Medicine, Cairo University, Egypt.
author
text
article
2017
eng
Background and aims: The insulin pathway may play a role in development of colorectal cancer (CRC). In this study, we investigated associations between CRC and obesity in Egyptians with reference to single nucleotide polymorphisms (SNPs) in the insulin-like growth factor-1 (IGF-I) gene. We also studied serum levels of IGF-1in Egyptian CRC patients with different BMI values. Methods: This prospective study included 66 CRC patients and 30 healthy individuals, for whom body mass index (BMI) was estimated, patients and controls being categorized into overweight or obese in one group and average weight in the other. Serum levels of IGF-1 were assessed by ELISA and SNPs in the IGF-I gene at rs6214C/T, rs6220 T/C and rs35767 C/T were examined by PCR- RFLP. Results: Serum levels of IGF-1 were significantly lower in both CRC average weight and overweight cases. IGF-1 could negatively predict CRC at a cut-off of 154 ng/ml with 87.5% sensitivity and 72.6 specificity. IGF-1 rs6214 CT and TT (T allele) genotypes were associated with a significantly increased risk of CRC. Univariate logistic regression showed that CRC risk significantly decreases by 0.14 for each one unit increase in IGF1. Conclusion: BMI could be considered as effect modifier for CRC risk. IGF-1 SNP rs6214 (TT and CT) are significantly associated with risk regardless of the BMI.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
2959
2964
https://journal.waocp.org/article_51933_6c50458a127ad9d155f04613e6444cef.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.2959
Dosimetric Validation of Volumetric Modulated Arc Therapy (VMAT) Using AAPM TG-119 Benchmark Plans in an Upgraded CLINAC 2100CD for Flattening Filter Free (FFF) Photon Beams
Ashokkumar
Sangaiah
Research and Development center, Bharathiar University, Coimbatore-641046, India.
author
K M
Ganesh
Research and Development center, Bharathiar University, Coimbatore-641046, India.
author
K
Ramalingam
Research and Development center, Bharathiar University, Coimbatore-641046, India.
author
K
Karthikeyan
Research and Development center, Bharathiar University, Coimbatore-641046, India.
author
N
Jagadeeshkumar
Department of Radiation Oncology, Yashoda Hospitals, Secunderabad-500003, India.
author
text
article
2017
eng
Background: Recently we have upgraded our Varian Clinac 2100CD with a 6MV FFF beam, this upgrade being the first of its kind in our country. Even though the dosimetric characteristics of FFF beams have been reported both in experimental and Monte Carlo studies, application in planning and delivery is complex. The aim of this study was to validate the commissioning of upgraded FFF beams dosimetrically using AAPM TG-119 bench mark plans for VMAT and to make a comparison with IMRT plans for both flattened filtered and FFF beams. Materials and Methods: AAPM TG-119 proposes a set of test clinical cases for testing the accuracy of IMRT planning and delivery systems. For these clinical cases we generated four treatment plans using IMRT FF, IMRT FFF, VMAT FF and VMAT FFF on a Varian Clinac 2100CD machine equipped with a millennium 120 MLC in Eclipse treatment planning system. Dose prescription and planning objectives were set according to the TG-119 goals and plans were scored based on planning objectives. Plans were compared using dose coverage, the conformity index and the homogeneity index. Point doses were measured at points recommended by TG-119 using a CC13 ion chamber. Planar dosimetry was accomplished using Imatrix and gamma evaluation was conducted using Omnipro IMRT software. Results: Dose distributions of FFF beam based plans were comparable to FF plans for both IMRT and VMAT. Our planning results matched TG-119 planning results. Measured point doses were within ±2% of planned doses and planar dosimetry gamma values were 95% of data points for all plans. Conclusion: We found a reduction of 40% treatment time for FFF against FF beams for sliding window IMRT. Upgraded FFF beams were in good agreement with TG-119 benchmark plans and goals.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
2965
2970
https://journal.waocp.org/article_51770_b811fe8644513395d312eb0d56982e98.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.2965
Breast Cancer Risk Assessment Using the Gail Model and It’s Predictors in Saudi Women
Hala Hazam
Al Otaibi
Department of Food Sciences and Nutrition, College of Agriculture and Food Science, King Faisal University, Saudi Arabia.
author
text
article
2017
eng
Background: The Gail Model has been widely implemented in developed and developing countries and is considered to be the best available instrument to estimate breast cancer (BC) risk for early prevention. Objective: The goals of the study were to determine five-year and lifetime BC risks and to assess BC predictors among female Saudi teachers using the Gail model. Methods: A cross sectional study with convenience sampling was conducted among 180 female Saudi secondary school teachers. The Gail model was used to evaluate the five-year and lifetime risks of developing BC. Included were a one-day 24-hour recall to assess daily serving sizes and food groups for food intake and questions regarding daily exercise, BMI, and demographic data. Result: The mean age of the teachers was 41±7.2 years, with a 0.87±0.93 mean for the five-year risk and a 9.6±5.4 mean lifetime risk of developing BC. According to the general linear model, the BC risk predictors were age, age at menarche, age at first pregnancy, family history, BMI, fruit and vegetable intake, and meat intake. Conclusion: The present study provided new information regarding the potential factors for five-year and lifetime invasive BC risk among Saudi women. Moreover, we could confirm that the Gail model is an appropriate BC risk assessment tool for Saudi women for early prevention, particularly among women at high risk of BC.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
2971
2975
https://journal.waocp.org/article_51771_4cfa37930222c9ca7ea29926d0b15a82.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.2971
Effect of Targeted Therapy With Pazopanib on Expression Levels of Transcription, Growth Factors and Components of AKT/m-TOR Signaling Pathway in Patients with Renal Cell Carcinoma
Liudmila V
Spirina
Cancer Research Institute, Tomsk National Research Center, Russian Academy of Medical Sciences, Tomsk, Russia.
author
Evgeny A
Usynin
Cancer Research Institute, Tomsk National Research Center, Russian Academy of Medical Sciences, Tomsk, Russia.
author
Zahar A
Yurmazov
Cancer Research Institute, Tomsk National Research Center, Russian Academy of Medical Sciences, Tomsk, Russia.
author
Elena M
Slonimskaya
Cancer Research Institute, Tomsk National Research Center, Russian Academy of Medical Sciences, Tomsk, Russia.
author
Irina V
Kondakova
Cancer Research Institute, Tomsk National Research Center, Russian Academy of Medical Sciences, Tomsk, Russia.
author
text
article
2017
eng
Background: The effect of the targeted therapy on cancer molecular markers remains currently unknown. The aim of the study was to investigate the expression and content of transcription, growth factors and components of the AKT/m-TOR signaling pathway in kidney cancer patients before and after targeted therapy with pazopanib. Methods: A total of 157 patients with renal cell carcinoma were enrolled into the study. The level of mRNA expression was investigated by real-time PCR, and the contents of transcription and growth factors, as well as the levels of AKT/m- TOR signaling pathway components were determined by ELISA and Western blotting. Results: Targeted therapy with pazopanib resulted in a 3.1-fold decrease in HIF-2α expression that was accompanied by a reduction in the levels of NF-κB p65 and p50, HIF-1α and CAIX. The levels of GSK-3ß and AKT mRNA were increased; however, the levels of corresponding proteins remained low. The targeted therapy with pazopanib did not influence the level of PTEN phosphatase. A 1.9-fold increase in the level of p70 S6 (S371) was observed after therapy. Conclusion: The efficacy of tyrosine kinase inhibitors is associated with the changes in the angiogenic factors. Molecular characteristics of cancer could determine markers of disease progression as well as potential targets for anticancer therapies
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
2977
2983
https://journal.waocp.org/article_51937_43ec1b4857979e5b78c095ebae2a6c7b.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.2977
Positive Impact of Social Media Use on Depression in Cancer Patients
Hamid Reza
Farpour
Bone and Joint Diseases Research Center, Department of Physical Medicine and Rehabilitation, Shiraz University of Medical Sciences, Shiraz, Iran.
author
Leila
Habibi
Department of Management, University of Tehran, Tehran, Iran.
author
Seyed Hossein
Owji
tudent Research Committee, Shiraz,Shiraz University of Medical Sciences, Shiraz, Iran.
author
text
article
2017
eng
Objective: The focus of attention was the prevalence of depression among cancer patients using social networks. An attempt was made to determine if social media could help cancer patients overcome their stress and depression, causes of serious emotional and mental problems for them and their families. Methods: To ascertain the prevalence of depression among cancer patients with reference to use of social networks, 316 cancer patients in the Association of Cancer Patients and cancer-related centers in Tehran at 2015 were evaluated. Depression was measured using the Beck Depression Inventory. Data were analyzed by the Chi-square test with SPSS software. Results: Using the Beck criteria, 61% (N=192) of patients were depressed. Interestingly, a significant difference was observed between depression in users and non-users of social networks (p=0.001), 33.9% and 66.1% being affected, respectively. Conclusion: These results verified a high incidence of depression in cancer patients, but a beneficial effect of social network use. Therefore access to social networks should be promoted for prevention and amelioration of depression. Moreover, it is recommended that particular attention be paid to the patient sex and educational level in designing counseling and psychological skill training programs.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
2985
2988
https://journal.waocp.org/article_51772_ec07b607dc42821ba54592764301bc03.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.2985
18-Fluorodeoxy-Glucose Positron Emission Tomography- Computed Tomography (18-FDG-PET/CT) for Gross Tumor Volume (GTV) Delineation in Gastric Cancer Radiotherapy
Kinga
Dębiec
Radiotherapy and Chemotherapy I Clinic, Maria Skłodowska-Curie Memorial Institute of Oncology, Gliwice Branch, Poland.
author
Jerzy
Wydmański
Department of Radiotherapy, Maria Skłodowska-Curie Memorial Institute of Oncology, Gliwice Branch, Poland.
author
Izabela
Gorczewska
Department of PET Diagnostics, Maria Skłodowska-Curie Memorial Institute of Oncology, Gliwice Branch, Poland.
author
Paulina
Leszczyńska
Department of Radiotherapy Planning, Maria Skłodowska-Curie Memorial Institute of Oncology, Gliwice Branch, Poland.
author
Kamil
Gorczewski
Department of PET Diagnostics, Maria Skłodowska-Curie Memorial Institute of Oncology, Gliwice Branch, Poland.
author
Wojciech
Leszczynski
Department of Radiotherapy Planning, Maria Skłodowska-Curie Memorial Institute of Oncology, Gliwice Branch, Poland.
author
Andrea
d’Amico
Department of PET Diagnostics, Maria Skłodowska-Curie Memorial Institute of Oncology, Gliwice Branch, Poland.
author
Michał
Kalemba
Department of Nuclear Medicine and Endocrine Oncology, Maria Skłodowska-Curie Memorial Institute of Oncology, Gliwice Branch, Poland.
author
text
article
2017
eng
Purpose: Evaluation of the 18-fluorodeoxy-glucose positron emission tomography-computed tomography (18-FDG-PET/CT) for gross tumor volume (GTV) delineation in gastric cancer patients undergoing radiotherapy. Methods: In this study, 29 gastric cancer patients (17 unresectable and 7 inoperable) were initially enrolled for radical chemoradiotherapy (45Gy/25 fractions + chemotherapy based on 5 fluorouracil) or radiotherapy alone (45Gy/25 fractions) with planning based on the 18-FDG-PET/CT images. Five patients were excluded due to excess blood glucose levels (1), false-negative positron emission tomography (1) and distant metastases revealed by 18-FDG-PET/CT (3). The analysis involved measurement of metabolic tumor volumes (MTVs) performed on PET/CT workstations. Different threshold levels of the standardized uptake value (SUV) and liver uptake were set to obtain MTVs. Secondly, GTVPET values were derived manually using the positron emission tomography (PET) dataset blinded to the computed tomography (CT) data. Subsequently, GTVCT values were delineated using a radiotherapy planning system based on the CT scans blinded to the PET data. The referenced GTVCT values were correlated with the GTVPET and were compared with a conformality index (CI). Results: The mean CI was 0.52 (range, 0.12-0.85). In 13/24 patients (54%), the GTVPET was larger than GTVCT, and in the remainder, GTVPET was smaller. Moreover, the cranio-caudal diameter of GTVPET in 16 cases (64%) was larger than that of GTVCT, smaller in 7 cases (29%), and unchanged in one case. Manual PET delineation (GTVPET) achieved the best correlation with GTVCT (Pearson correlation = 0.76, p <0.0001). Among the analyzed MTVs, a statistically significant correlation with GTVCT was revealed for MTV10%SUVmax (r = 0.63; p = 0.0014), MTVliv (r = 0.60; p = 0.0021), MTVSUV2.5 (r = 0.54; p = 0.0063); MTV20%SUVmax (r = 0.44; p = 0.0344); MTV30%SUVmax (r = 0.44; p = 0.0373). Conclusion: 18-FDG-PET/CT in gastric cancer radiotherapy planning may affect the GTV delineation.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
2989
2998
https://journal.waocp.org/article_51914_c5ccffe0a81303f730278068b69c0642.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.2989
Association between ERCC1 Polymorphism and the Risk and Clinicopathological Features of Breast Cancer in Thai Women in the Lower Northeastern Region
Malinee
Pongsavee
Department of Medical Technology, Faculty of Allied Health Sciences,
Thammasat University, Patumthani, Thailand.
author
Kamol
Wisuwan
Graduate program in Medical Technology, Faculty of Allied Health Sciences,
Thammasat University, Patumthani, Thailand.
author
Danai
Tiwawech
Department of Anatomy, Faculty of Medical Science, Naresuan University,
Phitsanulok, Thailand.
author
text
article
2017
eng
Background: Breast cancer is a major public health problem around the world, including Thailand and it has the highest ranking among female cancer. Currently, the diversity or polymorphism of ERCC1 gene (excision repair cross-complementary group 1 gene or ERCC1) was reported to associate with an increased risk of breast cancer. This study aims to investigate the relationship between ERCC1 polymorphism and the breast cancer risk in the lower northeastern region women of Thailand. Materials and Methods: One hundred fifty one samples from breast cancer patients and 120 samples from healthy control group were analysed. Genomic DNA was extracted from white blood cell of all samples. The real-time polymerase chain reaction (qPCR) was used to demonstrate genetic polymorphism of ERCC1. Results: The results showed that the ERCC1 rs11615 polymorphism variant AG was associated with an increased risk of breast cancer. This study demonstrated that the frequency of ERCC1 rs11615 in patients with breast cancer was higher than healthy control group. The ERCC1 polymorphism variant AG carrier presented 3.53-folds high risk of breast cancer [odds ratio (OR) = 3.53, 95% CI = 1.61-7.74, P = 0.001]. In addition, when age, menopause period, number of child, smoking and alcohol drinking were adjusted, the ERCC1 rs11615 variant AG carrier was associated with increased breast cancer risk to 3.97 folds, with OR = 3.79, 95% CI = 1.62-8.84, P = 0.002. Conclusions: This study showed that ERCC1 rs11615 genotype AG was associated with breast cancer risk in the lower northeastern region women of Thailand.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
2999
3002
https://journal.waocp.org/article_51168_ce9bcc7c79d5da6513a7e37b910f9219.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.2999
Promoter Hypermethylation of the ATM Gene as a Novel Biomarker for Breast Cancer
Nasrin
Begam
Jawaharlal Nehru Institute of Advanced Studies (JNIAS), School of Life Sciences, Centre for Biotechnology and Bioinformatics, Secunderabad- 500003,Telangana, India.
author
Kaiser
Jamil
Jawaharlal Nehru Institute of Advanced Studies (JNIAS), School of Life Sciences, Centre for Biotechnology and Bioinformatics, Secunderabad- 500003,Telangana, India.
author
Suryanarayana G
Raju
Nizam's Institute of Medical Sciences, Department of Surgical Oncology, Hyderabad, Telangana, India.
author
text
article
2017
eng
Background: Breast cancer may be induced by activation of protooncogenes to oncogenes and in many cases inactivation of tumor suppressor genes. Ataxia telangiectasia mutated (ATM) is an important tumor suppressor gene which plays central roles in the maintenance of genomic integrity by activating cell cycle checkpoints and promoting repair of double-strand breaks of DNA. In breast cancer, decrease ATM expression correlates with a poor outcome; however, the molecular mechanisms underlying downregulation are still unclear. Promoter hypermethylation may contribute in downregulation. Hence the present investigation was designed to evaluate promoter methylation and expression of the ATM gene in breast cancer cases, and to determine links with clinical and demographic manifestations, in a South Indian population. Methods: Tumor biopsy samples were collected from 50 pathologically confirmed sporadic breast cancer cases. DNA was isolated from tumor and adjacent non-tumorous regions, and sodium bisulfite conversion and methylation-specific PCR were performed using MS-PCR primers for the ATM promoter region. In addition, ATM mRNA expression was also analyzed for all samples using real-time PCR. Results: Fifty eight percent (58%) of cancer tissue samples showed promoter hypermethylation for the ATM gene, in contrast to only 4.44% of normal tissues (p= 0.0001). Furthermore, ATM promoter methylation was positively associated with age (p = 0.01), tumor size (p=0.045) and advanced stage of disease i.e. stages III and IV (p =0.019). An association between promoter hypermethylation and lower expression of ATM mRNA was also found (p=0.035). Conclusion: We report for the first time that promoter hypermethylation of ATM gene may be useful as a potential new biomarker for breast cancer, especially in the relatively young patients.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
3003
3009
https://journal.waocp.org/article_51915_c1530ac5c6340f76f246655073f8bd7c.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.3003
Sonographic Diagnosis of Tubal Cancer with IOTA Simple Rules Plus Pattern Recognition
Theera
Tongsong
Department of Obstetrics and Gynecology, Chiang Mai University, Thailand.
author
Chanane
Wanapirak
Department of Obstetrics and Gynecology, Chiang Mai University, Thailand.
author
Charuwan
Tantipalakorn
Department of Obstetrics and Gynecology, Chiang Mai University, Thailand.
author
Dangcheewan
Tinnangwattana
Department of Obstetrics and Gynecology, Chiang Mai University, Thailand.
author
text
article
2017
eng
Objective: To evaluate diagnostic performance of IOTA simple rules plus pattern recognition in predicting tubal cancer. Methods: Secondary analysis was performed on prospective database of our IOTA project. The patients recruited in the project were those who were scheduled for pelvic surgery due to adnexal masses. The patients underwent ultrasound examinations within 24 hours before surgery. On ultrasound examination, the masses were evaluated using the well-established IOTA simple rules plus pattern recognition (sausage-shaped appearance, incomplete septum, visible ipsilateral ovaries) to predict tubal cancer. The gold standard diagnosis was based on histological findings or operative findings. Results: A total of 482 patients, including 15 cases of tubal cancer, were evaluated by ultrasound preoperatively. The IOTA simple rules plus pattern recognition gave a sensitivity of 86.7% (13 in 15) and specificity of 97.4%. Sausage-shaped appearance was identified in nearly all cases (14 in 15). Incomplete septa and normal ovaries could be identified in 33.3% and 40%, respectively. Conclusion: IOTA simple rules plus pattern recognition is relatively effective in predicting tubal cancer. Thus, we propose the simple scheme in diagnosis of tubal cancer as follows. First of all, the adnexal masses are evaluated with IOTA simple rules. If the B-rules could be applied, tubal cancer is reliably excluded. If the M-rules could be applied or the result is inconclusive, careful delineation of the mass with pattern recognition should be performed.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
3011
3015
https://journal.waocp.org/article_51938_261b5e548a851473a2e2ffdccba113db.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.3011
Delay in Diagnosis and Treatment of Breast Cancer among Women Attending a Reference Service in Brazil
Tiara Cristina
Romeiro Lopes
Department of Health Science, Faculty Post Graduate in Health Science, State University Maringa, Parana, Brazil.
author
Angela Andréia França
Gravena
Department of Health Science, Faculty Post Graduate in Health Science, State University Maringa, Parana, Brazil.
author
Marcela de Oliveira
Demitto
Nursing Department,State University Maringa, Parana, Brazil.
author
Deise Helena Pelloso
Borghesan
Department of Health Science, Faculty Post Graduate in Health Science, State University Maringa, Parana, Brazil.
author
Cátia Millene
Dell Agnolo
Health Sciences, State University Maringa, Parana, Brazil.
author
Sheila Cristina Rocha
Brischiliari
Department of Health Science, Faculty Post Graduate in Health Science, State University Maringa, Parana, Brazil.
author
Maria Dalva de Barros
Carvalho
Department of Health Science, Faculty Post Graduate in Health Science, State University Maringa, Parana, Brazil.
author
Sandra Marisa
Pelloso
Department of Health Science, Faculty Post Graduate in Health Science, State University Maringa, Parana, Brazil.
author
text
article
2017
eng
Background: Cancer is a major public health problem. Early diagnosis and treatment are essential for reducing mortality. This study aimed to analyze factors associated with delay in breast cancer diagnosis and treatment among women attending a reference cancer service. Methods: This retrospective, cross-sectional study was performed with data collected from medical records and interviews conducted with women diagnosed with breast cancer and treated from October 2013 to October 2014 at a cancer reference hospital in Paraná, Southern Brazil. Results: A total of 82 participants were enrolled during the study period; their average age was 58.2 ± 11.5 years. The average time taken for final diagnosis of breast cancer was 102.5 ± 165.5 days. Treatment onset was delayed in the majority of cases, and the average time elapsing from diagnostic biopsy to onset of primary treatment was 72.3 ± 54.0 days. The odds of treatment delay were higher among the women with a low educational level. Conclusions: The results underline the need for proposals aimed at early detection, identification of risk factors and timely provision of treatment by health managers that focus on this group.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
3017
3023
https://journal.waocp.org/article_51773_5b645b74784185faa7d8336bd4ae6b5d.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.3017
Interleukin-6 Genetic Variation and Susceptibility to Gastric Cancer in an Iranian Population
Marzieh
Attar
Medical Cellular and Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
author
Maryam
Mansoori
Medical Cellular and Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
author
Majid
Shahbazi
Medical Cellular and Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
author
text
article
2017
eng
Background: Despite recent decrease in the incidence of gastric cancer, it is still a common type of cancer in the north of Iran. Many evaluations have shown that polymorphisms of cytokine genes like that for interleukin 6 (IL-6), which play important roles in regulation of the immune response, can increase the risk of gastric cancer. This study examined the role of the IL-6-174 gene polymorphism in susceptibility in an Iranian population. Method: Genomic DNA was extracted from peripheral whole blood of 100 patients and 361 healthy controls. Genotyping was accomplished by the sequence-specific primer-polymerase chain reaction (SSP-PCR) method and statistical analyses were carried out using Fisher’s exact test. Frequencies of the IL-6-174 G/C genotypes were determined under co-dominant, dominant, and recessive genetic models. Results: An association between the polymorphism of IL-6 -174 G/C and susceptibility to gastric cancer was observed. The frequency of G allele was higher in patients (78%) than in controls (70.5 %) (OR=1.48, 95% CI=1.01-2.20, P=0.04). Conclusions: The high G allele and G/G genotype frequency in patients compared to control subjects suggests that the IL-6 -174 G/C polymorphism may influence the susceptibility to gastric cancer. In addition, the demographic information showed that most of the subjects were male (69.0%) that gastric cancer is related to environmental factors.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
3025
3029
https://journal.waocp.org/article_51776_6da71d1b61dadeb6a3b2944d15306d32.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.3025
FLT3-ITD, NPM1, and DNMT3A Gene Mutations and Risk Factors in Normal Karyotype Acute Myeloid Leukemia and Myelodysplastic Syndrome Patients in Upper Northern Thailand
Piyanan
Mevatee
Department of Anatomy, Faculty of Medicine, Chiang Mai University, Thailand.
author
Adisak
Tantiworawit
Division of Hematology, Department of Internal Medicine, Faculty of Medicine, Chiang Mai University, Thailand.
author
Patrinee
Traisathit
Department of Statistics, Faculty of Science, Chiang Mai University, Thailand.
author
Chaniporn
Puaninta
Department of Anatomy, Faculty of Medicine, Chiang Mai University, Thailand.
author
Umnat
Mevatee
Department of Anatomy, Faculty of Medicine, Chiang Mai University, Thailand.
author
Sirinda
Angsuchawan
Department of Anatomy, Faculty of Medicine, Chiang Mai University, Thailand.
author
Kanokkan
Bumroongkit
Department of Anatomy, Faculty of Medicine, Chiang Mai University, Thailand.
author
text
article
2017
eng
Objective: Approximately 40-45% of AML and MDS patients have a cytogenetically normal karyotype (CN-AML and CN-MDS). The frequency and types of gene mutations in these cases may differ among various populations. The objective of this study was to identify frequencies and types of FLT3-ITD, NPM1, and DNMT3A mutations, and associations of them with clinical data and risk factors in CN-AML and CN-MDS cases in upper Northern Thailand. Methods: Bone marrow samples of 40 CN-AML and 60 CN-MDS patients were analyzed for gene mutations by direct sequencing. In addition, data for potential risk factors were obtained for comparison. Results: Frequencies of FLT3-ITD, NPM1, and DNMT3A mutations were 25.0%, 17.5%, and 10.0%, respectively in CN-AML, but all zero in CN-MDS cases. NPM1 mutations were found at a median age older than the wild type (58 vs 47 years) while DNMT3A mutations were associated with an increase in the white blood cell count. In all patients, factors for the mutations of these three genes included age ≤ 60 years, and a history of hypertension. Conclusion: When considering mutations in only normal karyotype patients, the frequency of FLT3-ITD, NPM1, DNMT3A mutations in CN-AML patients in upper Northern Thailand were found to occur at lower rates than in Western patients and to differ from other Asian populations including parts of Thailand. No mutations were observed in CN-MDS cases. Some types of gene mutations differed from previous studies, possibly attributable to differences in geography, lifestyle and genetic backgrounds. Links with age ≤ 60 years and history of hypertension were found. Investigation of these three genes in an intermediate risk group with a normal karyotype is useful for a better understanding of molecular leukemogenetic steps in CN-AML and CN-MDS patients and may be beneficial for planning treatment and prevention in the population of upper Northern Thailand.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
3031
3039
https://journal.waocp.org/article_51940_3d34b3725f96160aa9b1b6629cf33d40.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.3031
DNA Replication Licensing Proteins for Early Detection of Lung Cancer
Veena
VS
Division of Pathology, Regional Cancer Centre, Medical College Campus, Thiruvananthapuram, India.
author
Rajan
K
Respiratory Medicine, Medical College,Thiruvananthapuram, India.
author
Saritha
VN
Division of Cancer Research, Regional Cancer Centre, Medical College Campus, Thiruvananthapuram, India.
author
Preethi
Sara George
Division of Cancer Epidemiology and Biostatistics, Regional Cancer Centre, Medical College Campus, Thiruvananthapuram, India.
author
Chandramohan
K
Surgical Oncology, Regional Cancer Centre, Medical College Campus, Thiruvananthapuram, India.
author
Jayasree
K
Division of Pathology, Regional Cancer Centre, Medical College Campus, Thiruvananthapuram, India.
author
Thara
S
Division of Pathology, Regional Cancer Centre, Medical College Campus, Thiruvananthapuram, India.
author
K
Sujathan
Division of Cancer Research, Regional Cancer Centre, Medical College Campus, Thiruvananthapuram, India.
author
text
article
2017
eng
Background: To identify and characterize malignant and premalignant cells in sputum and matched tissue samples with reference to expression of minichromosome maintenance proteins (MCM2, MCM5) and cell division cycle protein 6 (CDC 6) and to assess their potential as biomarkers of premalignant and malignant lesions of the lung and associations with clinicopathological features. Methods: Expression of MCM2, MCM5 and 6 proteins in sputum samples and corresponding tissues was assessed by immunocytochemistry, and correlated with histological findings. Results: For characterization of malignant, metaplastic or dysplastic cells, CDC6 protein had the highest sensitivity of 87.7%. All the three markers together had a sensitivity of 94.4%. Furthermore these proteins could be employed to assess the proliferative potential of precancerous or atypical cells, as overexpression increasing with the stage of disease and degree of metastasis. Conclusion: The assessed markers can be utilized in routine cytopathology laboratories to supplement conventional morphological evaluation so that the sensitivity of sputum cytology can be enhanced. Potential applications in predicting the clinical behavior of lung lesions and predicting prognosis and survival deserve further attention.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
3041
3047
https://journal.waocp.org/article_51929_fa12939c9275fc1932540e0acb40c5b9.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.3041
P16ink4a Subcellular Expression Patterns in Colorectal Adenocarcinoma, Adenoma and Non-Neoplastic Tissue Samples
Enam Alhagh
Charkhat Gorgich
Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences,
Zahedan, Iran.
author
Zahra
Heidari
Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences,
Zahedan, Iran.
author
Hamidreza
Mahmoudzadeh-Sagheb
Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences,
Zahedan, Iran.
author
text
article
2017
eng
Background: Colorectal cancer (CRC) is one of the most common neoplasms with high mortality at advanced stages worldwide. Thus diagnosis of CRC at an early stage with sensitive molecular methods is a high priority. The aim of this study was to evaluate P16ink4a subcellular expression patterns in colorectal adenocarcinoma, adenoma and non-neoplastic tissue samples. Methods: A total of 137 colorectal formalin fixed paraffin-embedded tissue blocks from the pathology archives of Ali-Ebne-Abitaleb central hospital, Zahedan, Iran, were examined in three groups: adenocarcinoma (n= 63), adenoma (n= 38) and non-neoplastic (n= 36). The subcellular expression pattern was determined by immunocytochemistry. Data analysis was performed using Kruskal-Wallis and Fisher exact tests with the significance level set as p˂0.05. Results: P16ink4a subcellular localization was observed in three different patterns, nuclear+cytoplasmic (73.33%), cytoplasmic (13.33%) and nuclear (13.33%). In most samples, nuclear+cytoplasmic was the predominant subcellular pattern. However, a significant difference in P16ink4a subcellular expression patterns was observed along the non-neoplastic, adenoma, adenocarcinoma sequence (p˂0.001). An association with the histological tumor type was also noted (p=0.021). Conclusion: Considering variation in localization of P16ink4a under different pathological conditions, P16ink4a night be sensitive prognostic biomarker for benign colon lesions. Its use may improve strategies for screening, prognostic assessment and management of patients with CRC. Further studies are recommended in this field.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
3049
3054
https://journal.waocp.org/article_51930_741e12399796ba3d02585d06b59800b6.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.3049
Anti-Vascular Endothelial Growth Factor Targeting by Curcumin and Thalidomide in Acute Myeloid Leukemia Cells
Mahdieh
Salemi
Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran.
author
saeed
mohammadi
Hematology Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran.
author
Ardeshir
Ghavamzadeh
Hematology Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran.
author
Mohsen
Nikbakht
Hematology Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran.
author
text
article
2017
eng
Acute myeloid leukemias (AMLs) are blood disorders that exhibit uncontrolled growth and reduction of apoptosis rates. As with other malignancies, progression may be result of induction and formation of new blood vessels influenced by disease conditions. Cancer cells produce a variety of factors which play important roles in angiogenesis. Vascular endothelial growth factor (VEGF) is critical for many malignancies, including AMLs. Curcumin, as a natural compound, is able to enhance apoptosis via a mechanism affecting regulatory genes. As a new strategy we here evaluated anti- VEGF properties of curcumin, alone and in combination with thalidomide, in leukemic cell lines. Growth inhibitory effects were assessed by MTT assay and apoptosis was detected by annexin/PI staining in U937 and KG-1 cell lines. mRNA expression levels of VEGF isoforms were evaluated by qRT-PCR. Curcumin inhibited proliferation and induced apoptosis in both KG-1 and U937 cells and this effect was stronger in combination with thalidomide. In KG-1 cells, the level of VEGF (A, B, C and D) mRNA was decreased in curcumin-treated as compared to untreated cells. Maximum effects were obtained at the concentration of 40 μM curcumin in U937 cells. Taken together, the results indicate that the VEGF autocrine loop may have an impact on AML development and progression and could be considered as a therapeutic target. Thalidomide as a VEGF inhibitor in combination with curcumin appears to have a synergistic impact on inhibition of cell proliferation and promotion of apoptosis.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
3055
3061
https://journal.waocp.org/article_51179_39e609aae9ff56e43c18f379ab6fa1c2.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.3055
Relationships of Ex-Vivo Drug Resistance Assay and Cytokine Production with Clinicopathological Features in the Primary Cell Culture of Thai Ovarian and Fallopian Tube Cancer Patients
May Thuu
Mon
Center for Research and Development of Natural Products for Health, Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
author
Supachai
Yodkeeree
Center for Research and Development of Natural Products for Health, Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
author
Wanisa
Punfa
Center for Research and Development of Natural Products for Health, Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
author
Sonthaya
Umsumarng
Center for Research and Development of Natural Products for Health, Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
author
Suree
Lekwanavijit
Department of Pathology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
author
Sumalee
Siriaunkgul
Department of Pathology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
author
Prapaporn
Suprasert
Department of Gynecology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
author
Pornngarm
Limtrakul
Center for Research and Development of Natural Products for Health, Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
author
text
article
2017
eng
Objective: Our goal was to determine the ex-vivo drug resistance assay, as well as the cytokine production, in response to platinum-based chemotherapy treatment in primary culture cells established from the tumor tissue of ovarian or fallopian tube carcinoma patients, and to predict the clinical responses to chemotherapy. Methods: Sensitivity to the platinum-based drug was analyzed in two ovarian cancer cell lines and 19 tumor samples using the primary cell culture obtained from 19 patients having ovarian or fallopian tube cancer that had undergone surgery from 2014 to 2017. Results: Our findings in the ovarian cancer cell lines showed that SKOV3 cells displayed 10-fold greater resistance to cisplatin and 5.8 times more resistance to carboplatin than A2780 cells. SKOV3 cells displayed platinum-induced IL-6 and IL-8 overproduction whereas wild type A2780 displayed no detectable cytokine production. Regarding the primary cell culture obtained from patients, ex-vivo drug resistance assay results revealed that although extreme drug resistance was correlated with late stage ovarian cancer (P= 0.031), it could not independently predict or alter the outcomes of patients with ovarian or fallopian tube cancer. No relationship was found between basal cytokine secretion and the clinical parameters. However, carboplatin-induced IL-6 and IL-8 production had a significant association with the clinical response to chemotherapy (P=0.016 and P=0.038 respectively). Carboplatin-induced IL-8 overproduction was correlated with FIGO staging III-IV (P=0.026), but no correlation between carboplatin-induced IL-6 and FIGO staging (P= 0.061) was noted. Conclusion: These results suggest that cytokine production in response to platinum-based chemotherapy in primary culture cells may be useful as a predictive marker for the therapeutic outcomes among ovarian or fallopian tube cancer patients.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
3063
3071
https://journal.waocp.org/article_51942_a927e069cb0f3fa057e390256ff49029.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.3063
SNP rs16969968 as a Strong Predictor of Nicotine Dependence and Lung Cancer Risk in a North Indian Population
Namita
Pandey
Department of Genetics, University of Delhi South Campus, New Delhi, India.
author
Soumyadip
Pal
author
Lokesh
Sharma
Department of Biochemistry, Dr Ram ManoharLohia Hospital, Baba Kharak Singh Marg, New Delhi, Delhi, India.
author
Randeep
Guleria
Department of Pulmonary Medicine and Sleep Disorder, All India Institute of Medical
Sciences, New Delhi, Delhi, India.
author
Anant
Mohan
Department of Pulmonary Medicine and Sleep Disorder, All India Institute of Medical
Sciences, New Delhi, Delhi, India.
author
Tapasya
Srivastava
Department of Genetics, University of Delhi South Campus, New Delhi, India.
author
text
article
2017
eng
Background: The 15q24-25 loci contain genes (CHRNA5 and CHRNA3) encoding nicotinic acetylcholine receptor subunits. We here determined for the first time the association of genetic variants rs16969968 and rs3743074 in CHRNA5 and CHRNA3, respectively, on nicotine dependence and lung cancer risk in a North Indian population by a case-control approach. Methods: Venous blood samples were obtained from 324 participants (108 lung cancer patients and 216 healthy individuals). DNA was extracted and PCR amplified with primers flanking the SNPs rs16969968 and rs3743074. Amplicons were subjected to sequencing and logistic regression was used to analyze association between variables. Results: The risk variant SNP rs16969968 in both heterozygous and homozygous forms appeared to exert a significant effect on nicotine dependence [GA (OR=2.77) and AA (OR=2.53)]. As expected, smoking was strongly associated with lung cancer (OR= 2.62). Risk allele rs16969968 in CHRNA5 also showed a significant association with increased lung cancer risk in our cohort, alone (OR= 4.99) and with smoking as a co-variable (OR= 4.28). Comparison of our analysis with other populations suggested that individuals with rs16969968 risk allele in the Indian population are more susceptible to lung cancer. Conclusion: Overall, the results strongly indicated that, in our cohort North Indian population, the genetic variant rs16969968, but not rs3743074, is significantly associated with both nicotine dependence and increased risk of lung cancer. While the results are significant, there is further need to increase the sample size and improve precision of our risk prediction.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
3073
3079
https://journal.waocp.org/article_51781_c787a5eecd568f66b0defe11ff96ab3c.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.3073
Primary Locations of Malignant Melanoma Lesions Depending on Patients’ Gender and Age
Karolina
Stanienda-Sokol
Department of Ophthalmology, Regional Specialist Hospital No. 3 in Rybnik, Rybnik, Poland.
author
Natalia
Salwowska
Department of Dermatology, Andrzej Mielęcki Memorial Independent Public Clinical Hospital in Katowice, Poland.
author
Martyna
Slawinska
Department of Dermatology, Venereology and Allergology, Medical University of Gdańsk, Poland
author
Katarzyna
Wicherska-Pawlowska
Department of Hematology, Blood Cancers and Marrow Transplants, Independent Public Teaching Hospital No. 1 in Wrocław, Poland
author
Anna
Lorenc
District Railway Hospital in Katowice, Poland
author
Dominika
Wcislo-Dziadecka
Department of Dermatology, Andrzej Mielęcki Memorial Independent Public Clinical Hospital in Katowice, Poland.
author
Jerzy
Wydmanski
Radiotherapy Department, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Poland
author
Wojciech
Majewski
Radiotherapy Department, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Poland
author
text
article
2017
eng
Introduction: Location of malignant melanoma lesions depends on environmental, genetic, sociological and demographical factors. Available sources do not provide enough information on such dependencies in various populations. There is no data concerning the role of socio-demographic factors for the population of the Central and Eastern Europe. Aim: The aim of this work was to evaluate the anatomical location of the primary malignant melanoma lesion in correlation to patients’ gender and age. Material and methods: A retrospective analysis of medical documentation of 363 patients has been performed. The patients had been diagnosed with malignant melanoma and were undergoing treatment in the years 2010-2014 in two Polish oncologic hospitals. The subject group consisted of 199 (55%) females and 164 (45%) males. The age varied between 19 - 90 years, with the median of 62 years. Results: In women, the melanoma lesions seem to appear more often in their lower extremities, while in case of men such lesions seem to be more often on their torsos. In both cases, the difference was statistically significant (ppatients. The lesions located on heads and necks were most common in older patients, and the lesions located in lower extremities were most common in younger ones. Conclusion: Differences in location of malignant melanoma lesions may be due to either genetic or environmental reasons. It is often emphasized in literature that correlation between the socio-demographic factors and the process of oncogenesis requires intensive research. In our work, we have tried to fill this gap for the population of Central and Eastern Europe to determine the exact epidemiology of this kind of cancer. This knowledge may be then used for developing cancer prevention methods specific to gender and age.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
3081
3086
https://journal.waocp.org/article_51774_a499f9650325a7f4c122774230ee4cdc.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.3081
Repair Index in Examination of Nuclear Changes in the Buccal Mucosa of Smokers: A Useful Method for Screening of Oral Cancer
Sareh
Farhadi
Oral and Maxillofacial Pathology Department, Dental Branch of Tehran, Islamic Azad University, Tehran, Iran.
author
Maryam
Mohamadi
Oral and Maxillofacial Pathology Department, Dental Branch of Tehran, Islamic Azad University, Tehran, Iran.
author
Mehdi
Mohamadi
Oral and Maxillofacial Pathology Department, Dental Branch of Tehran, Islamic Azad University, Tehran, Iran.
author
text
article
2017
eng
Background: Smoking is one of the major risk factors for cancers, especially in the oral cavity. Nuclear changes occur in the early stages of cancer. The aim of this study was therefore to investigate nuclear changes and calculate a "repair index" for the buccal mucosa of smokers. Material and Methods: This historical cohort study was conducted by selecting samples including smokers and non-smokers. In addition, the smoker group were divided into 2 subgroups with a smoking history of >10 and ≤10 years. Buccal mucosa smears were obtained and Papanicolaou staining was employed to detect nuclear changes. Micronuclei, karyorrhexis and karyolysis were assessed and eventually a repair index was calculated. Statistical analysis was performed using the t-test. Results: In the 60 samples studied, differences were significant in smopkers vs. nonsmokers for micronuclei, (P=0.002) but not karyorrhexis or karyolysis. (P=0.789 and P=0.578, respectively). Also, the repair index demonstrated no statistically significant variation (P=0.107). Comparison of the two subgroups of smokers demonstrated that the frequency of micronuclei in those with a history >10 years was significantly higher and the RI was significantly lower than with ≤10 years (P=0.0001 and 0.04, respectively). While karyorrhexis and karyolysis were also higher in the longer exposure individuals the differences were not significant (P=0.07 and 0.78, respectively). Conclusion: Among the nuclear changes investigated, micronuclei proved the more reliable indicator to assess the adverse effects of smoking on the oral mucosa, becoming prominent with increase in smoking history. In addition, while a "repair index" may have benefits for assessment of nuclear damage caused by smoking, further research is necessary in this field.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
3087
3090
https://journal.waocp.org/article_51169_be44c31014bb3701f347b2504aaa0ade.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.3087
Inhibitory Effect of Aspirin on Cholangiocarcinoma Cells
Parichart
Boueroy
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
author
Ratchadawan
Aukkanimart
Neglected, Zoonosis and Vector-Borne Disease Group, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
author
Thidarut
Boonmars
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
author
Pranee
Sriraj
Neglected, Zoonosis and Vector-Borne Disease Group, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
author
Panaratana
Ratanasuwan
Department of Anesthesiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
author
Amornrat
Juasook
Faculty of Veterinary Medicine, Mahasarakham University, Maha Sarakham 44000, Thailand.
author
Nadchanan
Wonkchalee
Neglected, Zoonosis and Vector-Borne Disease Group, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
author
Kulthida
Vaeteewoottacharn
Cholangiocarcinoma Research Institue, Cholangiocarcinoma Screening and Care Program (CASCAP), Khon Kaen University, Khon Kaen 40002, Thailand.
author
Sopit
Wongkham
Cholangiocarcinoma Research Institue, Cholangiocarcinoma Screening and Care Program (CASCAP), Khon Kaen University, Khon Kaen 40002, Thailand.
author
text
article
2017
eng
Aspirin and other non-steroidal anti-inflammatory drugs reduce the risk of cancer due to their anti-proliferative and apoptotic effects, which are the important mechanisms for their anti-tumor activity. Here, the effect of aspirin on human cholangiocarcinoma cells (KKU-214) and the underlying mechanisms of its action were explored. Cell proliferation was measured by sulforhodamine B (SRB) assay, while cell cycle distribution and apoptosis were determined by flow cytometry. Western blotting was used to explore protein expression underlying molecular mechanisms of anti-cancer treatment of aspirin. Aspirin reduced cell proliferation in a dose- and time-dependent manner, and altered the cell cycle phase distribution of KKU-214 cells by increasing the proportion of cells in the G0/G1 phase and reducing the proportion in the S and G2/M phases. Consistent with its effect on the cell cycle, aspirin also reduced the expression of cyclin D1 and cyclin-dependent kinase 4 (Cdk-4), which are important for G0/G1 cell cycle progression. Treatment with aspirin led to increased induction of apoptosis in a dose-dependent manner. Further analysis of the mechanism underlying the effect of this drug showed that aspirin induced the expression of the tumor-suppressor protein p53 while inhibiting the anti-apoptotic protein B-cell lymphoma-2 (Bcl-2). Correspondingly, the activation of caspase-9 and -3 was also increased. These findings suggest that aspirin causes cell cycle arrest and apoptosis, both of which could contribute to its anti-proliferative effect.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
3091
3096
https://journal.waocp.org/article_51649_7ed9b46b01e205a4304b975f69a1723d.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.3091
Body Mass Index and Breast Cancer Risk among Thai Premenopausal Women: a Case-Control Study
Wisit
Chaveepojnkamjorn
Department of Epidemiology, Faculty of Public Health, Mahidol University, Ratchathewi, Bangkok, Thailand.
author
Rungsinoppadol
Thotong
Research and Technology Assessment Division, National Cancer Institute, Bangkok, Thailand.
author
Pratana
Sativipawee
Department of Biostatistics, Faculty of Public Health, Mahidol University, Ratchathewi, Bangkok, Thailand.
author
Supachai
Pitikultang
Department of Family Health, Faculty of Public Health, Mahidol University, Ratchathewi, Bangkok, Thailand.
author
text
article
2017
eng
Background: Breast cancer (BC) is the leading malignancy in women with high incidence and mortality worldwide. Obesity is one of several established risk factors for chronic diseases including cancer. The objective of this research was to determine the association of body mass index (BMI) with BC among Thai premenopausal women (TPW). Materials and Methods: A case-control study was conducted among TPW attending the National Cancer Institute in Bangkok, with 257 cases and 257 controls in 2013-2014. Cases and controls were matched by age (± 5 years), residential area and duration of attending. Data were collected with a questionnaire comprising 2 parts: part 1 socio-demographic characteristics, and part 2 health risk behavior and reproductive factors and BMI. The obtained data were analyzed using descriptive and analytic statistics with a computerized statistical package. Results: The study participants were mainly 40-44 years old (60 %) with an average age of 39 years. The major type of BC was the invasive ductal carcinoma (91.8%). On univariate analysis, risk factors for BC among the TPW were family history of BC, history of benign breast tumors, younger age at menarche, parity, miscarriage, contraceptive use, passive smoking, multivitamin use, and BMI (p<0.05). Multivariable conditional logistic regression analysis, controlling for possible confounding factors, revealed that a BMI 25-29.9 and ≥ 30 kg/m2 increased the risk of BC by a factor of 2.09 and 2.37 times, respectively (OR=2.09, 95%CI =1.09-3.97; OR=2.37, 95%CI =1.24-10.06). Conclusions: A surveillance system of obesity should be conducted in cooperation with information regarding physical activities and weight control among TPW as an essential measure to reduce BC risk.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
3097
3101
https://journal.waocp.org/article_51779_c0d601075ee5419ee7c92e0870183f2d.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.3097
Epitope mapping of human HER2 specific mouse monoclonal antibodies using recombinant extracellular subdomains of HER2
Reza
Hosseini-Ghatar
Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
author
Tahereh
Soltantoyeh
Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
author
Motahareh
Bahadori
Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
author
Maryam
Golara
Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
author
Hadi
Hassannia
Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
author
Roya
Khosravi-Eghbal
Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
author
Jalal
Khoshnoodi
Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
author
Mohammad Ali
Judaki
Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
author
Forough
Golsaz-Shirazi
Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
author
Mahmood
Jeddi-Tehrani
Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
author
Mohammad Mehdi
Amiri
Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
author
Fazel
Shokri
Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
author
text
article
2017
eng
Background: Human epidermal growth factor receptor 2 (HER2) is overexpresed in several human malignancies. Numerous studies show that HER2 plays an important role in the development and maintenance of the malignant phenotype. Targetting of HER2 molecule by monoclonal antibodies (mAbs) is a promising therapeutic approach. Anti-HER2 mAbs affect cancer cells differently, targeting distinct epitopes of HER2. Methods: Reactivity of a panel of 8 mouse anti-HER2 mAbs was investigated by ELISA and Western blotting using different subdomains of the extracellular domain (ECD) of HER2. All subdomains of HER2, including I, II, III, IV, I+II, III+IV and full HER2-ECD were constructed and expressed in CHO cells. Cross-reactivity of the mAbs with other members of the human HER family and Cynomolgus HER2 was also studied by ELISA. The mAbs were also tested by immunohistochemistry (IHC) using HER2 positive breast cancer tissues. Results: Our results demonstrated that 3 out of 8 mAbs detected conformational epitopes (1T0, 2A8 and 1B5), while 5 mAbs identified linear epitopes (1F2, 1H9, 4C7, 1H6 and 2A9). Three of the mAbs recognized subdomain I, one mAb reacted with subdomain I+II, 2 mAbs recognized either subdomain III or IV and 2 mAbs recognized subdomain III+IV. However, none of our mAbs recognized subdomain II of HER2 alone. The mAbs displayed either inhibitory or stimulatory effect on HER2-overexpressing tumor cells and did not react with other members of the human HER family. The pattern of IHC results implies better reactivity of the linear epitopes recognizing mAbs. Conclusion: Our findings suggest that paired subdomains of HER2 are essential for mapping of mAbs recognizing conformational epitopes. Moreover, it seems to be no association between subdomain specificity and antitumor activity of our anti-HER2 mAbs.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
3103
3110
https://journal.waocp.org/article_53213_3096fe3774c37255d03954deccea7454.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.3103
Polycystic Ovarian Morphology may be a Positive Prognostic Factor in Patients with Endometrial Cancer who Achieved Complete Remission after Fertility-Sparing Therapy with Progestin
Yamato
Fukui
Department of Obstetrics and Gynecology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
author
Ayumi
Taguchi
Department of Obstetrics and Gynecology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
author
Katsuyuki
Adachi
Department of Obstetrics and Gynecology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
author
Marie
Sato
Department of Obstetrics and Gynecology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
author
Akira
Kawata
Department of Obstetrics and Gynecology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
author
Michihiro
Tanikawa
Department of Obstetrics and Gynecology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
author
Kenbun
Sone
Department of Obstetrics and Gynecology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
author
Mayuyo
Mori
Department of Obstetrics and Gynecology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
author
Kazunori
Nagasaka
Department of Obstetrics and Gynecology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
author
Yoko
Matsumoto
Department of Obstetrics and Gynecology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
author
Takahide
Arimoto
Department of Obstetrics and Gynecology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
author
Katsutoshi
Oda
Department of Obstetrics and Gynecology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
author
Yutaka
Osuga
Department of Obstetrics and Gynecology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
author
Tomoyuki
Fujii
Department of Obstetrics and Gynecology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
author
text
article
2017
eng
Background: The most studied fertility-sparing therapy for endometrial cancer (EC) is oral progestin therapy. However, complete remission (CR) rate after progestin therapy is not enough ranging from 60 to 80 %, with high recurrence rate. Clinical features that predict treatment efficacy and recurrence after progestin therapy have not yet been revealed in detail. The aim of this study was to investigate prognostic factors in patients with EC who achieved CR after medroxyprogesterone acetate (MPA) therapy. Methods: We retrospectively reviewed 35 EC patients treated with MPA at our institution between 2000 and 2016. Following confirmation of endometrioid adenocarcinoma G1, patients orally took 600 mg MPA daily for 26 weeks. Patients with CR periodically took oral contraceptives. The association of recurrence-free survival (RFS) with several clinical features including age, body mass index (BMI), and polycystic ovarian morphology (PCOM) was analyzed. Results: Of 35 patients, 25 (71%) achieved CR, whereas 10 (29%) underwent hysterectomy due to failure of MPA therapy. Eleven (44%) of 25 patients with CR successfully gave birth after MPA therapy, whereas 8 (32%) developed recurrence. On univariate analysis, PCOM was significantly associated with better recurrence-free survival (RFS) (P=0.009), and BMI ≥25 kg/m2 exhibited a nonsignificant trend for longer RFS (P=0.0674). Although multivariate analysis failed to detect any valid hazard ratio (HR), absence of PCOM and non-obesity were both independent risk factors for recurrence (P=0.00293 and P=0.0201, respectively). Notably, none of 10 cases with PCOM experienced recurrence under maintenance with oral contraceptives. Conclusion: PCOM might be a good prognostic factor in those achieving CR after MPA therapy for EC.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
3111
3116
https://journal.waocp.org/article_51928_0085bcec2fdb072b884ec5852eea3e29.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.3111
Prevalence of Abnormal Pap Smears: A Descriptive Study from a Cancer Center in a Low-Prevalence Community
Bayan
Maraqa
Department of Pathology King Hussein Cancer Center, (KHCC) Amman, Jordan.
author
Isam
Lataifeh
Department of Surgery,
King Hussein Cancer Center, (KHCC) Amman, Jordan.
author
Lian
Ottai
Department of Surgery,
King Hussein Cancer Center, (KHCC) Amman, Jordan.
author
Osama
Badran
Department of Surgery,
King Hussein Cancer Center, (KHCC) Amman, Jordan.
author
Yasar Qutaiba
Nouri
Early Detection/Community Outreach, King Hussein Cancer Center, (KHCC) Amman, Jordan.
author
Ibtihal
Issam
Early Detection/Community Outreach, King Hussein Cancer Center, (KHCC) Amman, Jordan.
author
Maysa
Al-Hussaini
Department of Pathology King Hussein Cancer Center, (KHCC) Amman, Jordan.
author
text
article
2017
eng
Background and aims: Cervical cancer is the fourth most common cancer in women worldwide and the 13th in Jordan. The cervical smear (Pap smear) is a simple approach to detect pre-cancerous cervical lesions. The aim of this study was to evaluate the prevalence of abnormal cervical smears in women seen at the Early Detection/Community Outreach clinic of King Hussein Cancer Center (KHCC). Materials and Methods: In this retrospective study, reports of routine cervical Pap smears performed in the pathology department at KHCC from January 2007 to December 2016, were reviewed. During this period, a total of 5,529 routine smears were assessed for epithelial abnormalities and histopathological grading. Results: A total of 210 (3.8%) abnormal Pap smears were found, with atypical squamous cell of undetermined significance (ASC-US) reported in 110 (52.4%) cases, atypical glandular cells of undermined significance (AGUS) in 58 (27.6%), low grade squamous intra-epithelial lesion (LSIL) in 27 (12.9%) and high grade intra-epithelial lesion (HSIL) in 13 (6.2%). Only single cases of ASC-H and squamous cell carcinoma were reported. The available biopsies showed benign findings in 70.1% of cases, low grade squamous intraepithelial lesions in 11.5% and high grade squamous intraepithelial lesions in 18.4%. Conclusions: The low epithelial cell abnormality (EPCA) prevalence illustrated in this study argues against introduction of population-based HPV testing and vaccination. It calls for a more cost-effective measures in a country with limited resources, where a more widely available Pap-smear testing might suffice.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
3117
3121
https://journal.waocp.org/article_51697_8744d7a1ccbd77c107c3b32108c6a235.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.3117
Prevalence of Helicobacter Pylori Infection in Dyspeptic Patients in Andkhoy Afghanistan
Mohammad Hussain
Hamrah
Curative Clinic, Andkhoy, Afghanistan.
author
Mohammad
Hamrah
Department of Cardiology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
author
Mohammad Hassan
Hamrah
Curative Clinic, Andkhoy, Afghanistan.
author
Mitsuro
Kanda
Department of Gastroenterological Surgery (Surgery II), Nagoya University Graduate, School of Medicine, Nagoya, Japan.
author
Ahmad Edris
Hamrah
Curative Clinic, Andkhoy, Afghanistan.
author
Ahmad Elias
Dahi
Curative Clinic, Andkhoy, Afghanistan.
author
Farhat
Homayoun
College of Stomatology, Kunduz University, Kunduz, Afghanistan.
author
Mohammad Hashem
Hamrah
Curative Clinic, Andkhoy, Afghanistan.
author
Akbar
Fotouhi
Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
author
Junichi
Sakamoto
Tokai Central Hospital, Kakamigahara, Japan.
author
text
article
2017
eng
Background: The prevalence of Helicobacter pylori (H. pylori) infection is reported to be more than 50% worldwide. It has been associated with peptic ulcer disease, gastric carcinoma, and mucosal-associated lymphoid tissue lymphoma. The seroprevalence of H. pylori varies greatly among societies and geographical locations. The objective of this study was to determine the seroprevalence and factors associated with H. pylori infection among dyspeptic patients in Afghanistan. Materials and Methods: This cross-sectional study was designed to determine the seroprevalence of H. pylori among dyspeptic patients in an outpatient clinic in Andkhoy, Afghanistan from January 2017 to April 2017. Data were obtained from patients using a questionnaire, including socio-demographic data and history of smoking and dyspeptic symptoms. Serum samples were also collected from the patients and tested for H. pylori antibodies using the enzyme-linked immunosorbent assay. Results: A total of 152 patients with dyspepsia were included in the analysis; 59 were men (40.0%), and 93 were women (60.0%). The overall seroprevalence of H. pylori was 75.6%. The predictor of seroprevalence of H. pylori was found to be illiteracy (odds ratio [OR] 4.99, 95% confidence interval [CI] 1.0324.17), epigastric pain (OR 12.01, 95% CI 3.56- 40.52), and rural population (OR 9.66, 95% CI 3.36- 27.82). There was an inverse association between employed status and seroprevalence of H. pylori (OR 0.08, 95% CI 0.01- 0.52). Conclusions: The seroprevalence of H. pylori was high among patient with dyspepsia in an outpatient clinic in Afghanistan. Illiteracy, unemployment and rural populations, and epigastric pain were significantly associated with H. pylori infection.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
3123
3127
https://journal.waocp.org/article_51457_a7ae7fa6a154fcaebbcde9fecc394274.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.3123
Multi-Gene Expression in Anthracosis of the Lungs as One of the Risk Factors for Non-Small Cell Lung Cancer
Hamidreza
Jamaati
Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran.
author
Naghmeh
Bahrami
Craniomaxillofacial Research Center, Tehran University of Medical Sciences,Tehran,Iran.
author
Payam
Tabarsi
Clinical Tuberculosis and Epidemiology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran.
author
Adnan
Khosravi
Tobacco Prevention and Control Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRI TLD),Shahid Beheshti University of Medical Sciences, Tehran, Iran.
author
Arda
Kiani
Tracheal Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran.
author
Atefeh
Abedini
Tracheal Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran.
author
Rahim
Ahmadi
Department of Physiology, Faculty of Basic Sciences, Hamedan Branch, Islamic Azad University, Hamedan, Iran.
author
Somayeh
Sharifynia
Clinical Tuberculosis and Epidemiology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran.
author
Abdolreza
Mohamadnia
Virology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran.
author
text
article
2017
eng
Background: Anthracosis of the lung occurs due to the deposition of carbon and silica in the mucosa and submucosa, manifested as black lesions. The association of anthracosis with lung cancer has remained to be clearly elucidated The current study aimed to assess the P16, CDH1 and LUNX genes expression level to evaluate the association of anthracotic lesions in the lungs with the occurrence of non-small cell lung cancer. Methods: Forty biopsy samples were taken from the center and 40 from the margins of black anthracotic lesions in the lungs; RNA was extracted from the samples and cDNA was synthesized. Real-time reverse-transcription polymerase chain reaction (RT-PCR) was performed to assess the expression of P16, CDH1 and LUNX genes. All steps were performed in triplicate. Results: A significant reduction in P16 gene expression was noted at the center compared to the margins of the lesions (P<0.001). expression level of CDH1 at the center of lesions was significantly lower than margins (P<0.001). However, LUNX gene had significantly higher expressionlevel at the center compared to margins (P<0.001). Conclusion: Decreased expression of P16 and CDH1 and increased expression of LUNX tumor genes were noted at the center of anthracotic lesions. Significant increase in expression of LUNX gene in NSCLC indicates an association between anthracosis and NSCLC, according to which, anthracotic patients may carry a high risk for NSCLC.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
3129
3133
https://journal.waocp.org/article_51948_ee5ba09319dc34a8dd3d9c964b3504b0.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.3129
The Utilization of Karyotyping, iFISH, and MLPA for the Detection of Recurrence Genetic Aberrations in Multiple Myeloma
Suchada
Sommaluan
Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
author
Budsaba
Rerkamnuaychoke
Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
author
Teeraya
Pauwilai
Division of Hematology, Department of Medicine, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
author
Suporn
Chuncharunee
Division of Hematology, Department of Medicine, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
author
Preeyaporn
Onsod
Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
author
Pitichai
Pornsarayuth
Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
author
Takol
Chareonsirisuthigul
Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
author
Rachaneekorn
Tammachote
Department of Botany, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.
author
Teerapong
Siriboonpiputtana
Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
author
text
article
2017
eng
Multiple myeloma (MM) is a hematological malignancy characterized by abnormal accumulation of clonal plasma cells in the bone marrow. Recently, multiplex ligation-dependent probe amplification (MLPA) has emerged as an effective and robust method for detection of common genetic alterations in MM patients. Here, we aimed to confirm MLPA utility for this purpose and furthermore to test the feasibility of a combination of karyotyping, interphase fluorescence in situ hybridization (iFISH) and MLPA methods for diagnosis, prognostic assessment and risk stratification of MM. Thirty-five genomic DNA samples isolated from CD138-enriched plasma cells from bone marrow of MM patients were analyzed using the MLPA method. We found that amp (1q) was the most frequent genetic alteration (48.6%) in the tested samples, followed by del (1p) and del (13q) (34.3%). Moreover, concordant results between sensitivity and specificity of iFISH and MLPA for the detection of del (13q) (p-value >0.05) and del (17p) (p-value >0.05) were obtained. In summary, we could provide evidence of MLPA assay utility for the detection of common genetic alterations in MM. The combination of karyotyping, iFISH, and MLPA proved very helpful for clinical risk stratification.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
3135
3142
https://journal.waocp.org/article_51458_7f7fc6ed14654e9c096e9019e72a20d8.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.3135
Detection of Juxtapleural Nodules in Lung Cancer Cases Using an Optimal Critical Point Selection Algorithm
sara
Saraswathi
MCA Department, St.Xavier’s College, Tamilnadu,India.
author
L MaryvImmaculate
Sheela
Research Supervisor Dilla University, Ethiopia.
author
text
article
2017
eng
Detection of lung cancer through image processing is an important tool for diagnosis. In recent years, image processing techniques have become more widely used. Lung segmentation is an essential pre-processing step for most(CAD) schemes. An automated system is proposed in this paper for identifying lung cancer from the analysis of computed tomography images by performing nodule segmentation using an optimal critical point selection algorithm (OCPS) which improves the detection of shape- and size-based juxtapleural nodules located at the lung boundary. A suspect area of nodule is obtained with the help of a bidirectional chain code (BDC) approach and the OCPS This algorithm is used to reduce the time consumption to detect the lung nodule and thereby reduce the computational complexity. Shape and size features are extracted for the area between two critical points to facilitate classification as nodule or non-nodule with the help of a support vector machine and random forest classifiers. This automated method was tested on computed tomography (CT) studies from the lung imaging database consortium (LIDC). The results are compared with the existing techniques using various performance measures such as precision rate, recall rate, accuracy and F-measure. The obtained experimental results indicate that the OCPS combined with a random forest classifier performs better in terms of performance evaluation metrics than existing approaches, with less requirement for computation.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
3143
3148
https://journal.waocp.org/article_51944_7ce20d3536560237609a8ebb00c5e9f0.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.3143
Oncologists’ Perspectives on Consolidation Radiation Treatment after Chemotherapy in Lymphoma: A Survey Study by the Lymphoma Working Committee of the Turkish Oncology Group (TOG)
Ozgur
Tanriverdi
Mugla Sitki Kocman University Faculty of Medicine, Department of Oncology
author
Ibrahim
Barista
Hacettepe University Oncology Institute, Department of Medical Oncology, Ankara, Turkey
author
Semra
Paydaş
Cukurova University Faculty of Medicine, Department of Medical Oncology, Adana, Turkey
author
Erdinc
Nayir
Necip Fazil City Hospital, Medical Oncology Clinic, Kahramanmaras, Turkey
author
Yusuf
Karakas
Hacettepe University Oncology Institute, Department of Medical Oncology, Ankara, Turkey
author
text
article
2017
eng
In this study, we aimed to determine the perspectives of medical and radiation oncologists on consolidation radiotherapy in patients with complete response after chemotherapy in Hodgkin’s and non-Hodgkin’s lymphoma. The survey was designed to identify demographic and occupational features of medical and radiation oncologists and their perspectives on consolidation radiotherapy in their clinical practices, as based on a five-point Likert scale (never, rarely, sometimes, often, and always). The study consisted of 263, out of 935, physicians working in the oncology field as either a medical or a radiation oncologist; the rate of return on the invitations to participate was 28%. The majority of the participants were male radiation oncologists, with a duration of between 5 and 10 years of work as a university hospital official, and the mean age was 38 ± 14 (years). Although the most commonly followed international guidelines were NCCN, among the physicians, the majority of the respondents suggested that the guidelines were unclear regarding recommendations for consolidative radiotherapy. The administered dose for consolidative radiotherapy in patients with lymphoma was indicated as 40 Gy by 49% of all the physicians and the most common cause of hesitancy from consolidative radiation treatment among the physicians was the risk of secondary malignancies as a long-term adverse effect (54%). In conclusion, we suggested that medical oncologists could be most active on the treatment of lymphoma through a continuous training program about lymphomas and current national guidelines.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
3149
3155
https://journal.waocp.org/article_53216_c9c8ded1a6c00b7a6ba157d4b296daa9.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.3149
Association of Voltage-Gated Sodium Channel Genetic Polymorphisms with Oxaliplatin-Induced Chronic Peripheral Neuropathy in South Indian Cancer Patients
Sreenivasulu
palugulla
Department of Pharmacology, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Pondicherry, India.
author
Dimpal N
Thakkar
Department of Pharmacology, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Pondicherry, India.
author
Smita
Kayal
Department of Medical Oncology, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Pondicherry, India.
author
Sunil
Narayan
Department of Neurology, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Pondicherry, India.
author
Steven
Dkhar
Department of Clinical Pharmacology, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Pondicherry, India.
author
text
article
2017
eng
Oxaliplatin is a platinum drug active against digestive tract cancers. Among its side effects, peripheral neuropathy is one of the dose-limiting toxicities. This affects around 50 to 70% of patients but the pathophysiology of development of oxaliplatin-induced peripheral neuropathy (OXAIPN) remains unclear. Sodium channels (SCNAs) play major role in neuronal electrical signaling processes and mutations in SCNAs lead to various neuronal diseases involving the central and peripheral nervous systems. In this study, we evaluated whether SCNA genetic variants might be associated with risk of chronic OXAIPN in patients with digestive tract cancers treated with oxaliplatin. Methodology: Blood samples from 228 digestive tract cancer patients who had received oxaliplatin in adjuvant and neoadjuvant or metastatic settings were obtained and genomic DNA was extracted by phenol-chloroform extraction. Genotyping was performed with the real-time polymerase chain reaction (RT-PCR) using validated real-time TaqMan single nucleotide polymorphism (SNP) genotyping assays. Neuropathy was evaluated and graded according to National Cancer Institute Common Toxicity Criteria (NCI-CTC) version 4.03. Results: We found that the rs6746030 polymorphic variant of SCN9A was significantly associated with a higher incidence of chronic OXAIPN (GA+AA vs GG: OR=1.8, 95% CI=1.04-3.4, P=0.04; dominant model) while the rs6754031 variant was linked with a lower incidence (OR=0.45, 95% CI=0.22-0.77, P=0.005; dominant model). The SCN 10A polymorphic variant was associated with severity of chronic OXAIPN (P=0.006, OR=2.0, 95% CI=1.2 - 3.3). Conclusion: The results of the present prospective study provide evidence in support of a causal relationship between chronic OXAIPN and voltage gated sodium channel polymorphisms. However, further studies from independent groups are required to validate these results.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
3157
3165
https://journal.waocp.org/article_51782_33b0b1384400a1d74e42a4a1fe8932b0.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.3157
Evaluation of Mir-224, Mir-215 and Mir-143 as Serum Biomarkers for HCV Associated Hepatocellular Carcinoma
Samah
Mamdouh
Department of Biochemistry and Molecular Biology, Theodor Bilharz Research Institute, Egypt.
author
Fatma
Khorshed
Department of Biochemistry and Molecular Biology, Theodor Bilharz Research Institute, Egypt.
author
Tarek
Aboushousha
Department of Pathology, Theodor Bilharz Research Institute, Giza, Egypt.
author
Hussam
Hamdy
Department of Surgery, Theodor Bilharz Research Institute, Giza, Egypt.
author
Ayman
Diab
Faculty of Biotechnology,October University for Modern Sciences and Arts, Giza, Egypt.
author
Mohamed
Seleem
Department of Surgery, National Hepatology and Tropical Medicine Research Institute, Cairo, Egypt.
author
Mohamed
Saber
Department of Biochemistry and Molecular Biology, Theodor Bilharz Research Institute, Egypt.
author
text
article
2017
eng
HCV induced hepatitis and hepatocellular carcinoma as its sequel are major health problems world-wide and especially in Egypt. For diagnosis and during treatment of liver diseases, liver functions are monitored through determination of serum levels of liver enzymes and α-fetoprotein although the obtained information is generally not sufficient for either early detection of hepatic insult or effective follow up of therapeutic effects. More sensitive biomarkers may help to achieve these goals. MiRNAs are small non-coding RNAs that have an important role in gene expression and regulation. Many, such as miR-224, miR-215, miR-143 are correlated with tumor appearance and with the degree of fibrosis in lung, breast and colon cancer. This study was performed to estimate the level of these miRNAs in serum of patients with HCV-associated hepatitis and HCC in relation to grade of hepatitis, stage of fibrosis and differentiation of tumor tissue. In addition, correlations between serological and tissue levels were assessed. A total of 80 patients were examined, out of which 50 were included in the study. Blood samples and tissue specimens from malignant tumor and corresponding non-tumor tissue of HCV hepatitis patients were collected. Blood samples from 20 healthy volunteers were also obtained as controls. It was found that miRNAs profiles differed in HCC patients compared to controls and HCV-associated hepatitis cases. Distinction of tumor grade and fibrosis stage of patients as well as between different grades of tumor differentiation proved possible, making miRNAs promising biomarkers for diagnosis and assessment of treatment response of HCC patients.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
3167
3171
https://journal.waocp.org/article_51945_c92a87d3fbe7f61fc2be84d7928c2143.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.3167
Significance of the Cytological Signs of Human Papillomavirus Infection in Anal Pap Smears of Human Immunodeficiency Virus-Infected Japanese Men Who Have Sex with Men
kaori
Okayama
School of Medical Technology, Faculty of Health Science, Gunma Paz University, Japan.
author
Mitsuaki
Okodo
2Department of Pathology, Faculty of Health Sciences, Kyorin University, Japan.
author
Hiroshi
Kitamura
Department of Medical Oncology, School of Medicine, Kyorin University, Japan.
author
Ichiro
Itoda
Shirakaba Clinic, Japan.
author
text
article
2017
eng
Purpose: The incidence of invasive anal cancer (IAC) has been increasing among human immunodeficiency virus (HIV)-positive men who have sex with men (MSM). Although cytological diagnosis is the modality of choice for screening cases of IAC, it is associated with lower sensitivity and specificity. Therefore, the present study aimed to evaluate new cytological signs of human papillomavirus (HPV) infection that may contribute to improving anal cytology. Methods: Anal cytology and HPV testing were performed using SurePath liquid-based cytology on samples obtained from 37 HIV-positive Japanese MSM. Subsequently, a histological biopsy based on high-resolution anoscopy was performed in MSM with abnormal cytological findings indicative of atypical squamous cells of undetermined significance (ASC-US) +. Also, anal Papanicolaou (Pap) smears were performed to determine cellularity, presence of dysplastic squamous cells, and other cytological signs of HPV infection. Results: Of the 37 MSM who underwent anal cytology, six tested negative for intraepithelial lesion or malignancy, three cases exhibited ASC-US, 17 exhibited low-grade squamous intraepithelial lesion (LSIL), nine exhibited high-grade squamous intraepithelial lesion (HSIL), and two remained undiagnosed. The anal Pap smears of 28 (96.6%) of the 29 MSM with abnormal cytological findings of ASC-US+ exhibited anal intraepithelial neoplasia (AIN), as revealed by histological biopsy. The median value (minimum–maximum) of the cellularity of anal Pap smears was 12 (0–70.5) nsc/hpf. In 26 MSM with LSIL and HSIL, the median dysplastic squamous cells count was 14 (2–152) dsc/smear and the cytological sign of HPV infection was 11 (2–71) hpv/smear. Of all anal Pap smears that revealed ASC-US+, 96.6% exhibited cytological signs of HPV infection. Compression-positive binucleated cells were the most prevalent among all cytological signs of HPV infection. Conclusion: For anal cytology, instead of considering a small number of dysplastic squamous cells, screening based on cytological signs of HPV infection may be beneficial for improving the diagnosis of AIN.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
3173
3178
https://journal.waocp.org/article_51777_e9280e16f5084afb04dbf614250092e3.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.3173
Impact of Smoking on Health-Related Quality of Life: A General Population Survey in West Iran
Satar
Rezaei
Research Center for Environmental Determinants of Health (RCEDH), Kermanshah University of Medical Sciences, Kermanshah,
Iran.
author
Behzad
Karami Matin
Research Center for Environmental Determinants of Health (RCEDH), Kermanshah University of Medical Sciences, Kermanshah,
Iran.
author
Ali
Kazemi Karyani
Research Center for Environmental Determinants of Health (RCEDH), Kermanshah University of Medical Sciences, Kermanshah,
Iran.
author
Abraha
Woldemichael
School of Public Health, College of Health Sciences, Mekelle University, Mekelle, Tigray, Ethiopia.
author
Farid
Khosravi
Research Center for Environmental Determinants of Health (RCEDH), Kermanshah University of Medical Sciences, Kermanshah,
Iran.
author
Masoud
Khosravipour
Research Center for Environmental Determinants of Health (RCEDH), Kermanshah University of Medical Sciences, Kermanshah,
Iran.
author
Shahab
Rezaeian
Research Center for Environmental Determinants of Health (RCEDH), Kermanshah University of Medical Sciences, Kermanshah,
Iran.
author
text
article
2017
eng
This study was aimed at assessing any association between smoking and health-related quality of life (HRQoL) among adults aged 18 years and above living in Kermanshah city, western Iran. A cross-sectional study was conducted on a total sample of 1,543 participants obtained by convenient sampling during the period from February 1st to May 30th, 2017. Data were collected using a self-administrated questionnaire. The HRQoL of the study participants was assessed with reference to the EuroQol 5-dimensions-3-level (EQ-5D-3L). The impact of smoking behavior of the participants on HRQoL with controls for potential confounders was examined by multiple regression. Out of the total of 1,543 participants, current smokers, past smokers, and never smokers accounted for 19.7%, 4.2% and 76.1%, respectively. The mean EQ-5D indices were 0.69 ±SD 0.20, 0.70 ± SD 0.22, and 0.78 ± SD 0.16. The highest proportion of self-reported problems (including both ‘some’ and ‘severe’) were related to current, heavy smokers, with high nicotine dependence. Regression analysis indicated that current smokers had a significantly lower HRQoL compared to past smokers and never smokers (p < 0.05). The heavy smokers also had a significantly lower HRQoL score than moderate and light smokers (p < 0.05) and there was an inverse relationship between the HRQoL score and nicotine dependence (p<0.05). The current smokers, heavy smokers, and high nicotine dependent smokers had lower HRQoL scores. These findings provide inputs for better understanding and for devising interventions for smoking cessation, reducing the number of cigarettes smoked per day and nicotine dependency.
Asian Pacific Journal of Cancer Prevention
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
1513-7368
18
v.
11
no.
2017
3179
3185
https://journal.waocp.org/article_51775_f345a8ebe1088e11d0ef880f74278a88.pdf
dx.doi.org/10.22034/APJCP.2017.18.11.3179