@article { author = {}, title = {Punarnavine Induces Apoptosis in B16F-10 Melanoma Cells by Inhibiting NF-kB Signaling}, journal = {Asian Pacific Journal of Cancer Prevention}, volume = {10}, number = {6}, pages = {1031-1032}, year = {2009}, publisher = {West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.}, issn = {1513-7368}, eissn = {2476-762X}, doi = {}, abstract = {The objective of this study was to assess the effect of Punarnavine, an alkaloid isolated from Boerhaaviadiffusa, on apoptosis in B16F-10 melanoma cells. Treatment of B16F-10 melanoma cells with nontoxicconcentrations of Punarnvine resulted in the presence of apoptotic bodies and DNA fragmentation in a dosedependent manner. Cell cycle analysis and TUNEL assays also confirmed the observation. The apoptotic genesp53 and caspase-3 were found upregulated in Punarnavine treated cells, whereas the antiapoptotic gene Bcl-2was downregulated. The inhibited nuclear translocation of NF-κBp65, NF-κBp50, NF-κB-c-Rel, c-Fos, ATF-2and CREB-1 in Punarnavine treated B16 F-10 cells pointed to suppression of NF-κB signaling by Punarnavine.All these results demonstrate that Punarnavine induces apoptosis via activation of p53 induced caspase-3 mediatedpro-apoptotic signaling and suppression of NF-κB induced Bcl-2 mediated survival signaling.}, keywords = {Punarnavine,apoptosis induction,B16F-10 melanoma cells,NF-κB inhibition}, url = {https://journal.waocp.org/article_25053.html}, eprint = {https://journal.waocp.org/article_25053_3ac04b71f674c365174f67511fc41e55.pdf} }