@article { author = {}, title = {TP53 - Molecular Soldier’s Mutations in Bladder Cancer in the Kashmiri Population}, journal = {Asian Pacific Journal of Cancer Prevention}, volume = {12}, number = {1}, pages = {67-72}, year = {2011}, publisher = {West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.}, issn = {1513-7368}, eissn = {2476-762X}, doi = {}, abstract = {Purpose: We made a preliminary attempt to study mutations in exons 5-8 (the DNA binding domain) ofthe tumor suppressor gene TP53, in urinary bladder cancer patients from Kashmir. Further the relation ofclinicopathological characteristics with mutation status was asessed. Materials and Methods: The study populationconsisted of 60 patients diagnosed with transitional cell carcinomas who underwent transurethral resection and/or radical cystectomy. Mutations in 5-8 exons of TP53 gene were detected by means of single strand conformationpolymorphism (SSCP). All samples which showed different migration bands in SSCP were confirmed by DNAsequencing. Results: 19 of 60 (31.6%) bladder cancers had mutations of the TP53 gene (11 transitions and 8transversions), three were G→A transitions, two G→T transversions, three A→C transversions, five C→Ttransitions and six A→T transversions. Predominantly missense mutations (66%) were detected but no deletionsor insertions were found. Statistically significant associations (<0.05) were noted with higher tumor stage (T2 orhigher), recurrence and large tumor size (>3cm). No correlation was found between smoking and tumor gradeand the presence of TP53 mutations. Conclusions: Mutation of the TP53 gene is one of the commonest geneticchanges in human bladder cancer, also in a high risk ethnic Kashmiri population.}, keywords = {TP53 mutations,SSCP,bladder cancer,Kashmir}, url = {https://journal.waocp.org/article_25475.html}, eprint = {https://journal.waocp.org/article_25475_29be49f640990a76f405511cd13d5277.pdf} }