@article { author = {}, title = {RNA Interference of Caveolin-1 Via Lentiviral Vector Inhibits Growth of Hypopharyngeal Squamous Cell Carcinoma FaDu Cells In Vitro and In Vivo}, journal = {Asian Pacific Journal of Cancer Prevention}, volume = {12}, number = {2}, pages = {397-401}, year = {2011}, publisher = {West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.}, issn = {1513-7368}, eissn = {2476-762X}, doi = {}, abstract = {Objective: To investigate the effects of caveolin-1 (CAV1) on the growth of hypopharyngeal squamous cellcarcinoma (HSCC) FaDu cells in vitro and in vivo. Methods: A CAV1-RNAi-lentivirus construct was transfectedinto FaDu cells and expression of caveolin-1 was tested by RT-PCR and western blotting analysis. Cell apoptosiswas analyzed by transferase-medisated dUTP nick-end labeling (TUNEL) assay. Tumor inhibition effects wereinvestigated by injecting rCAV1-RNAi-lentivirus construct into tumors created with FaDu cells in the HSCCmouse model, with the empty-vector lentivirus as a control. CAV1 expression in xenografts was tested by RT-PCRand immunohistochemistry. Results: RT-PCR and western blot analysis demonstrated successful construction ofthe CAV1-RNAi-lentivirus construct producing small hairpin RNA. The average weights and volumes of tumorin mice treated with CAV1-RNAi-lentivirus were lower than in mice with control treatment (P<0.05). RT-PCRrevealed weak positive expression of CAV1 in CAV1-construct–treated xenografts and immunohistochemistryconfirmed lower CAV1 expression than in controls.(P<0.05). In addition, downregulation of CAV1 increased cellapoptosis in vitro. Conclusion: The growth of HSCCs could be inhibited by recombinant CAV1-RNAi-lentivirusin vitro and in vivo.}, keywords = {caveolin-1,Lentivirus,RNA interference,Hypopharyngeal carcinoma cells}, url = {https://journal.waocp.org/article_25532.html}, eprint = {https://journal.waocp.org/article_25532_0a924374a35d38387d05121db181885f.pdf} }