@article { author = {}, title = {Triptolide Inhibits Proliferation and Induces Apoptosis of Human Melanoma A375 Cells}, journal = {Asian Pacific Journal of Cancer Prevention}, volume = {13}, number = {4}, pages = {1611-1615}, year = {2012}, publisher = {West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.}, issn = {1513-7368}, eissn = {2476-762X}, doi = {}, abstract = {Triptolide, a diterpenoid obtained from Tripteryglum wilfordii Hook.f, has attracted interest for its antitumoractivities against human tumor cell lines in recent years. This report focuses on anti-proliferative andpro-apoptotic activities in human melanoma A375 cells assessed by CCK8 assay, Hoechst 33258 staining andflow cytometry. In addition, triptolide-induced arrest in the S phase was also observed. Caspase assays showedthe apoptosis induced by triptolide was caspase-dependent and probably through intrinsic apoptotic pathways.Furthermore, expression of NF-kB (p65) and its downstream factors such as Bcl-2, Bcl-XL was down-regulated.Taken together, the data indicate that triptolide inhibits A375 cells proliferation and induces apoptosis by acaspase-dependent pathway and through a NF-kB-mediated mechanism.}, keywords = {Triptolide,melanoma A375 cells,Apoptosis,caspase,NF-KB}, url = {https://journal.waocp.org/article_26381.html}, eprint = {https://journal.waocp.org/article_26381_e0e7068f4dd8356d9dd57751c8b2d966.pdf} }