@article { author = {}, title = {Proteomics Analysis of Gastric Epithelial AGS Cells Infected with Epstein-Barr Virus}, journal = {Asian Pacific Journal of Cancer Prevention}, volume = {14}, number = {1}, pages = {367-372}, year = {2013}, publisher = {West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.}, issn = {1513-7368}, eissn = {2476-762X}, doi = {}, abstract = {Effects of the Epstein-Barr virus (EBV) on cellular protein expression are essential for viral pathogenesis. Tocharacterize the cellular response to EBV infection, differential proteomes of gastric epithelial AGS cellswereanalyzed with two-dimensional gel electrophoresis (2-DE) followed by matrix-assisted laser desorption/ionizationtimeof flight (MALDI-TOF) and liquid chromatography electrospray/ionization ion trap (LC–ESI–IT) massspectrometry identification. Mass spectrometry identified 9 altered cellular proteins, including 5 up-regulatedand 4 down-regulated proteins after EBV infection. Notably 2-DE analysis revealed that EBV infection inducedincreased expression of heat shock cognate 71 kDa protein, actin cytoplasmic 1, pyridoxine-5’-phosphate oxidase,caspase 9, and t-complex protein 1 subunit alpha. In addition, EBV infection considerably suppressed thosecellular proteins of zinc finger protein 2, cyclin-dependent kinase 2, macrophage-capping protein, and growth/differentiation factor 11. Furthermore, the differential expressional levels of partial proteins (cyclin-dependentkinase 2 and caspase 9) were confirmed by Western blot analysis.Thus, this work effectively provided usefulprotein-related information to facilitate further investigation of the mechanisms underlying EBV infection andpathogenesis.}, keywords = {Epstein-Barr Virus,gastric epithelial cells,Two-dimensional gel electrophoresis,Mass spectrometry}, url = {https://journal.waocp.org/article_27333.html}, eprint = {https://journal.waocp.org/article_27333_7957f05d6e05f6b7c34db345a7d018e7.pdf} }