@article { author = {}, title = {Let-7c Inhibits NSCLC Cell Proliferation by Targeting HOXA1}, journal = {Asian Pacific Journal of Cancer Prevention}, volume = {14}, number = {1}, pages = {387-392}, year = {2013}, publisher = {West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.}, issn = {1513-7368}, eissn = {2476-762X}, doi = {}, abstract = {Objective: The aim of the present study was to explore mechanisms by which let-7c suppresses NSCLC cellproliferation. Methods: The expression level of let-7c was quantified by qRT-PCR. A549 and H1299 cells weretransfected with let-7c mimics to restore the expression of let-7c. The effects of let-7c were then assessed by cellproliferation, colony formation and cell cycle assay. Mouse experiments were used to confirm the effect of let-7con tumorigenicity in vivo. Luciferase reporter assays and Western blotting were performed to identify targetgenes for let-7c. Results: HOXA1 was identified as a novel target of let-7c. MTS, colony formation and flowcytometry assays demonstrated that forced expression of let-7c inhibited NSCLC cell proliferation by inducingG1 arrest in vitro, consistent with inhibitory effects induced by knockdown of HOXA1. Mouse experimentsdemonstrated that let-7c expression suppressed tumorigenesis. Furthermore, we found that let-7c could regulatethe expression of HOXA1 downstream effectors CCND1, CDC25A and CDK2. Conclusions: Collectively, theseresults demonstrate let-7c inhibits NSCLC cell proliferation and tumorigenesis by partial direct targeting ofthe HOXA1 pathway, which suggests that restoration of let-7c expression may thus offer a potential therapeuticintervention strategy for NSCLC.}, keywords = {Let-7c,NSCLC,HOXA1,G1 arrest}, url = {https://journal.waocp.org/article_27336.html}, eprint = {https://journal.waocp.org/article_27336_9b8e5279f2ca3fcdb18c34a313340678.pdf} }