@article { author = {}, title = {Effects of miR-155 Antisense Oligonucleotide on Breast arcinoma Cell Line MDA-MB-157 and Implanted Tumors}, journal = {Asian Pacific Journal of Cancer Prevention}, volume = {14}, number = {4}, pages = {2361-2366}, year = {2013}, publisher = {West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.}, issn = {1513-7368}, eissn = {2476-762X}, doi = {}, abstract = {Diverse studies have shown that miR-155 is overexpressed in different tumor types. However, the precisemolecular mechanism of the ectopic expression of miR-155 in breast cancer is still poorly understood. Tofurther explore the role of miR-155 in breast tumorigenesis, we here assessed the influence of miR-155 antisenseoligonucleotide (miR-155 ASO) on MDA-MB-157 cell viability and apoptosis in vitro. Furthermore, the effectsof inhibitory effects of miR-155 on the growth of xenograft tumors in vivo were determined with performanceof immunohistochemistry to detect expression of caspase-3, a pivotal apoptosis regulatory factor, in xenografts.Transfection efficiency detected by laser confocal microscope was higher than 80%. The level of miR-155expression was significantly decreased (P<0.05) in the cells transfected with miR-155 ASO, compared withthat in cells transfected with a negative control. After being transfected with miR-155 ASO, the viability ofMDA-MB-157 cells was reduced greatly (P<0.05) and the number of apoptotic cells was increased significantly.Additionally, miR-155 ASO inhibited the growth of transplanted tumor in vivo and significantly increased theexpression of caspase-3. Taken together, our study revealed that miR-155 ASO can induce cell apoptosis andinhibit cell proliferation in vitro. Moreover, miR-155 ASO could significantly repress tumor growth in vivo,presumably by inducing apoptosis via caspase-3 up-regulation. These findings provide experimental evidencefor using miR-155 as a therapeutic target of breast carcinoma.}, keywords = {Breast Neoplasms,microRNA-155,antisense oligonucleotide,MDA-MB-157 cells,nude mice}, url = {https://journal.waocp.org/article_27648.html}, eprint = {https://journal.waocp.org/article_27648_b5f0ebf31f832de7bc6e5720eda34e93.pdf} }