@article { author = {}, title = {Inhibitory Effects of β-Cyclodextrin-Helenalin Complexes on H-TERT Gene Expression in the T47D Breast Cancer Cell Line - Results of Real Time Quantitative PCR}, journal = {Asian Pacific Journal of Cancer Prevention}, volume = {14}, number = {11}, pages = {6949-6953}, year = {2013}, publisher = {West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.}, issn = {1513-7368}, eissn = {2476-762X}, doi = {}, abstract = {Background: Nowadays, the encapsulation of cytotoxic chemotherapeutic agents is attracting interest as amethod for drug delivery. We hypothesized that the efficiency of helenalin might be maximized by encapsulationin β-cyclodextrin nanoparticles. Helenalin, with a hydrophobic structure obtained from flowers of Arnicachamissonis and Arnica Montana, has anti-cancer and anti-inflammatory activity but low water solubility andbioavailability. β-Cyclodextrin (β-CD) is a cyclic oligosaccharide comprising seven D-glucopyranoside units,linked through 1,4-glycosidic bonds. Materials and Methods: To test our hypothesis, we prepared β-cyclodextrinhelenalincomplexes to determine their inhibitory effects on telomerase gene expression by real-time polymerasechain reaction (q-PCR) and cytotoxic effects by colorimetric cell viability (MTT) assay. Results: MTT assayshowed that not only β-cyclodextrin has no cytotoxic effect on its own but also it demonstrated that β-cyclodextrinhelenalincomplexes inhibited the growth of the T47D breast cancer cell line in a time and dose-dependent manner.Our q-PCR results showed that the expression of telomerase gene was effectively reduced as the concentrationof β-cyclodextrin-helenalin complexes increased. Conclusions: β-Cyclodextrin-helenalin complexes exertedcytotoxic effects on T47D cells through down-regulation of telomerase expression and by enhancing Helenalinuptake by cells. Therefore, β-cyclodextrin could be superior carrier for this kind of hydrophobic agent.}, keywords = {Helenalin,Telomerase,real time quantitative PCR,β-cyclodextrin}, url = {https://journal.waocp.org/article_28393.html}, eprint = {https://journal.waocp.org/article_28393_ccaf493291e891d5c3272c01d2358eb3.pdf} }