@article { author = {}, title = {Berberine Hydrochloride Impact on Physiological Processes and Modulation of Twist Levels in Nasopharyngeal Carcinoma CNE-1 Cells}, journal = {Asian Pacific Journal of Cancer Prevention}, volume = {15}, number = {4}, pages = {1851-1857}, year = {2014}, publisher = {West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.}, issn = {1513-7368}, eissn = {2476-762X}, doi = {}, abstract = {Objective: The main purpose of this work was to investigate the effect of berberine hydrochloride (BH) onthe proliferation, apoptosis, migration, and invasion of CNE-1 nasopharyngeal carcinoma cells. Our results shedlight on the functional components of traditional Chinese herbs for potential use in modern medicine. Methods:The CNE-1 cell line was treated with different concentrations of BH and effects on cell viability and proliferationwere evaluated using the Cell Counting Kit-8 (CCK-8) assay. Anti-migratory and anti-invasive actions of BHwere investigated using wound healing assays and the Millicell Hanging cell culture insert system, respectively.Expression of the epithelial-mesenchymal transition (EMT)-related gene twist (Twist) was analyzed by real-timePCR and Western blotting. Apoptosis was estimated with an annexin-V fluorescein (FITC) apoptosis detectionkit, as well as with reference to levels of activated caspase-3 of CNE-1 cells before and after treatment with BHutilizing fluorescence spectroscopy. Results: BH was capable of reducing proliferation and viability of CNE-1cells in a dose- and time-dependent manner, also demonstrating anti-migratory and anti-invasive capacities whichcorrelated with reduction in expression of Twist. Finally, BH was able to induce significant amounts of apoptosisin CNE-1 cells, as demonstrated by an increase in the activity of caspase-3 and in annexin-V staining followingtreatment. Conclusion: BH extracted from rhizoma coptidis demonstrated an ability to block proliferation,induce apoptosis, and impair the migration and invasion of the CNE-1 cell line Considering these properties, ourresults suggest that BH could be an important compound for consideration in the treatment of nasopharyngealcarcinoma.}, keywords = {Berberine hydrochloride,Invasion,migration,Apoptosis,Cell proliferation,CNE-1}, url = {https://journal.waocp.org/article_28841.html}, eprint = {https://journal.waocp.org/article_28841_70f9562c7d78f960081915832b26ac88.pdf} }