@article { author = {}, title = {Preparation of Selenium-enriched Bifidobacterium Longum and its Effect on Tumor Growth and Immune Function of Tumor-Bearing Mice}, journal = {Asian Pacific Journal of Cancer Prevention}, volume = {15}, number = {8}, pages = {3681-3686}, year = {2014}, publisher = {West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.}, issn = {1513-7368}, eissn = {2476-762X}, doi = {}, abstract = {In this study, we demonstrated selenium (Se) accumulation in Bifidobacterium longum strain (B. longum)and evaluated the effect of Se-enriched B. longum (Se-B. longum) on tumor growth and immune function intumor-bearing mice. Analysis using high-performance liquid chromatography-inductively coupled plasmamass spectrometry (HPLC-ICP-MS) revealed that more than 99% of Se in Se-B. longum was organic, the maincomponent of which was selenomethionine (SeMet). In the in vivo experiments, tumor-bearing mice (n=8) wereorally administrated with different doses of Se-B. longum alone or combined with cyclophosphamide (CTX).The results showed that the middle and high dose of Se-B. longum significantly inhibited tumor growth. WhenSe-B. longum and CTX were combined, the antitumor effect was significantly enhanced and the survival time oftumor-bearing mice (n=12) was prolonged. Furthermore, compared with CTX alone, the combination of Se-B.longum and CTX stimulated the activity of natural killer (NK) cells and T lymphocytes, increasing the levels ofinterleukin-2 (IL-2) and tumor necrosis factor-α (TNF-α), and the leukocyte count of H22 tumor-bearing mice(n=12).}, keywords = {Bifidobacterium longum,Chemotherapy,selenium,selenium speciation,Tumor}, url = {https://journal.waocp.org/article_29139.html}, eprint = {https://journal.waocp.org/article_29139_0e9e1a5b003fbcbee9fef11b56570b18.pdf} }