@article { author = {}, title = {Induction of Indoleamine 2,3-dioxygenase (IDO) Enzymatic Activity Contributes to Interferon-Gamma Induced Apoptosis and Death Receptor 5 Expression in Human Non-small Cell Lung Cancer Cells}, journal = {Asian Pacific Journal of Cancer Prevention}, volume = {15}, number = {18}, pages = {7995-8001}, year = {2014}, publisher = {West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.}, issn = {1513-7368}, eissn = {2476-762X}, doi = {}, abstract = {Interferon-gamma (IFN-γ) has been used to treat various malignant tumors. However, the molecularmechanisms underlying the direct anti-proliferative activity of IFN-γ are poorly understood. In the present study,we examined the in vitro antitumor activity of IFN-γ on two human non-small-cell lung carcinoma (NSCLC) celllines, H322M and H226. Our findings indicated that IFN-γ treatment caused a time-dependent reduction in cellviability and induced apoptosis through a FADD-mediated caspase-8/tBid/mitochondria-dependent pathway inboth cell lines. Notably, we also postulated that IFN-γ increased indoleamine 2,3-dioxygenase (IDO) expressionand enzymatic activity in H322M and H226 cells. In addition, inhibition of IDO activity by the IDO inhibitor1-MT or tryptophan significantly reduced IFN-γ-induced apoptosis and death receptor 5 (DR5) expression,which suggests that IDO enzymatic activity plays an important role in the anti-NSCLC cancer effect of IFN-γ.These results provide new mechanistic insights into interferon-γ antitumor activity and further support IFN-γas a potential therapeutic adjuvant for the treatment of NCSLC}, keywords = {Non-small-cell lung carcinoma - nterferon-γ - apoptosis - indoleamine 2,3-dioxygenase - death receptor 5}, url = {https://journal.waocp.org/article_29873.html}, eprint = {https://journal.waocp.org/article_29873_ca01e28ae9785d48ea282d572051a285.pdf} }