@article { author = {}, title = {Characterisation and Clinical Significance of FLT3-ITD and non-ITD in Acute Myeloid Leukaemia Patients in Kelantan, Northeast Peninsular Malaysia}, journal = {Asian Pacific Journal of Cancer Prevention}, volume = {16}, number = {12}, pages = {4869-4872}, year = {2015}, publisher = {West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.}, issn = {1513-7368}, eissn = {2476-762X}, doi = {}, abstract = {Background: Mutations of the FMS-like tyrosine kinase-3 (FLT3) receptor gene may promote proliferationvia activation of multiple signaling pathways. FLT3-internal tandem duplication (FLT3-ITD) is the most commongene alteration found in patients diagnosed with acute myeloid leukaemia (AML) and has been associated withpoor prognosis. Materials and Methods: We performed mutational analysis of exons 14-15 and 20 of the FLT3gene in 54 AML patients using PCR-CSGE (conformational sensitive gel electrophoresis) followed by sequencinganalysis to characterise FLT3 mutations in adult patients diagnosed with AML at Hospital USM, Kelantan,Northeast Peninsular Malaysia. Results: FLT3 exon 14-15 mutations were identified in 7 of 54 patients (13%)whereas no mutation was found in FLT3 exon 20. Six ITDs and one non-ITD mutation were found in exon 14 ofthe juxtamembrane (JM) domain of FLT3. FLT3-ITD mutations were associated with a significantly higher blastpercentage (p-value = 0.008) and white blood cell count (p-value = 0.023) but there was no significant differencein median overall survival time for FLT3-ITD+/FLT3-ITD- within 2 years (p-value = 0.374). Conclusions: Theincidence of FLT3-ITD in AML patients in this particular region of Malaysia is low compared to the Westernworld and has a significant association with WBC and blast percentage.}, keywords = {AML,FLT3,ITD,CSGE}, url = {https://journal.waocp.org/article_31177.html}, eprint = {https://journal.waocp.org/article_31177_81fcb0bbda90c6654c23551864c48142.pdf} }