@article { author = {Sangkhamanon, Sakkarn and Jongpairat, Piangpen and Sookprasert, Aumkhae and Wirasorn, Kosin and Titapun, Attapol and Pugkhem, Ake and Ungareevittaya, Piti and Chindaprasirt, Jarin}, title = {Programmed Death-Ligand 1 (PD-L1) Expression Associated with a High Neutrophil/Lymphocyte Ratio in Cholangiocarcinoma}, journal = {Asian Pacific Journal of Cancer Prevention}, volume = {18}, number = {6}, pages = {1671-1674}, year = {2017}, publisher = {West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.}, issn = {1513-7368}, eissn = {2476-762X}, doi = {10.22034/APJCP.2017.18.6.1671}, abstract = {  Background: Effective treatments for cholangiocarcinoma (CCA) are still lacking. There are promising results of checkpoint inhibitor programmed cell death ligand-1 (PD-L1) activities in early phase trials. This study aimed to investigate the expression of PD-L1 and its relation to possible treatments for CCA. Methods: Formalin-fixed paraffin-embedded tumor samples from 46 patients with cholangiocarcinoma were retrieved. PD-L1 expression was evaluated by immunohistochemistry using anti-PD-L1 antibody, clone 5H1. A PD-L1 positive response on tumor cells was defined as >1% of tumor cell membranes stained. The association between PD-L1, clinico-pathological characteristics was analyzed using Fisher’s exact test, and survival analysis was done with the Cox regression model. Results: Out of 46 samples, 32 (70%) had positive PD-L1 expression in tumor cell membranes. The median level of PD-L1 expression was 1.75% (0-34.7). PD-L1 expression was significantly associated with stage IV disease (OR 3.98, p=0.046) and a high neutrophil/lymphocyte ratio (OR 5.36, p=0.018). PD-L1 positivity was associated with worse overall survival compared with those with a PD-L1 negative tumor but did not reach a level of significance (7.2 vs. 7.9 months, p=0.32). Conclusion: PD-L1 is widely expressed in CCA but was not predictive for overall survival. PD-L1 positivity was (7.2 and 7.9 months, p=0.32). Significantly associated with stage IV disease and a high neutrophil/lymphocyte ratio.}, keywords = {biliary tract cancer,cholangiocarcinoma,immune studies,Neutrophil-Lymphocyte ratio,PD-L1}, url = {https://journal.waocp.org/article_47597.html}, eprint = {https://journal.waocp.org/article_47597_75b5b8618779dc443bf0ac00984b49fb.pdf} }