@article { author = {Ostadrahimi, Shiva and Abedi-Valugerdi, Manuchehr and Hassan, Moustapha and Haddad, Ghazal and Fayaz, Shima and ParvizHamidi, Monireh and Mahdian, Reza and Fard-Esfahani, Pezhman}, title = {miR-1266-5p and miR-185-5p Promote Cell Apoptosis in Human Prostate Cancer Cell Lines}, journal = {Asian Pacific Journal of Cancer Prevention}, volume = {19}, number = {8}, pages = {2305-2311}, year = {2018}, publisher = {West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.}, issn = {1513-7368}, eissn = {2476-762X}, doi = {10.22034/APJCP.2018.19.8.2305}, abstract = {Objective: Small non-coding RNA molecules are dysregulated in prostate cancer (PCa). In our previous study,downregulation of miR-1266 and miR-185 was demonstrated in PCa tissues and cell lines. The aim of the presentstudy was to investigate whether miR-1266 and miR-185 are involved in the regulation of B-cell lymphoma (BCL) 2and BCL2L1, respectively, and whether transfection of PCa cell lines with miR-1266 and miR-185 mimics can altertumorigenic phenotypes. Methods: In order to investigate the regulation of BCL2 and BCL2L1 mRNA levels bymiR-1266 and miR-185, respectively, a luciferase reporter assay was used. Real-time PCR was also used to analyzechanges in the levels of BCL2 and BCL2L1 mRNAs in PCa cell lines following transfection with synthetic miR-1266and miR-185. Cell apoptosis was determined by Annexin V protein expression analysis via flow cytometry. In additionto the MTT assay, a cell proliferation assay was performed. Result: A luciferase assay confirmed that the BCL2 andBCL2L1 genes may be targeted by miR-1266 and miR-185, respectively, through binding to their 3′UTR regions.Transfection of PC3 and DU145 cells with miR-1266 and miR-185 induced apoptosis and reduced proliferation, whichalso revealed an inverse correlation with BCL2 and BCL2L1 gene expression in the treated cells. Conclusion: Ourdata suggests that miR-1266 and miR-185 may be novel candidates for further research in PCa treatment through theanti-apoptotic pathway.}, keywords = {Key words: miR-1266-5p,miR-185-5p,Prostate Cancer,BCL2,BCL2L1}, url = {https://journal.waocp.org/article_65374.html}, eprint = {https://journal.waocp.org/article_65374_248612b5736bdba90c105f770bb58ecd.pdf} }