@article { author = {Christudoss, Pamela and Chacko, Geeta and Selvakumar, Ratnasamy and Fleming, Jude J and Pugazhendhi, Srinivasan and Mathew, George}, title = {Expression of Metallothionein after Administration of Aspirin, Vitamin C or Zinc Supplement in the DMH Induced Colon Carcinoma in Rat}, journal = {Asian Pacific Journal of Cancer Prevention}, volume = {19}, number = {11}, pages = {3237-3244}, year = {2018}, publisher = {West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.}, issn = {1513-7368}, eissn = {2476-762X}, doi = {10.31557/APJCP.2018.19.11.3237}, abstract = {Background: Chemoprevention refers to the use of specificnatural or synthetic chemical agents to suppress thedevelopment and progression to carcinoma. The purpose of this study was to assess the effect of aspirin, vitamin Cor zinc on the metallothionein (MT) mRNA gene expression as well as MT protein content byimmunohistochemistryandradioimmunoassay (RIA) in 1, 2-dimethyl hydrazine (DMH) induced cancerous colonic tissuein rats. Methods:Rats were randomly divided into three groups, group 1 (aspirin), group 2 (vitamin C) group 3 (zinc), each of whichwas further sub divided into two groups and given subcutaneous injections of DMH (30 mg/kg body weight) twice aweek for 3 months and sacrificed at either 4 months (A-precancer model) or at 6 months (B-cancer model).The controlgroups were administered 0.5 ml saline subcutaneously. All the 3 groups were simultaneouslyadministered aspirin,vitamin Cor zinc supplement respectively from the beginning till the end of the study. Results: It was observed thatrats co-treated with aspirin, vitamin C or zinc resulted in a significant increase in the colonic MT mRNA expression inthe precancer and cancer model as compared to the saline only controls. MT protein expression showed a 60%, 64%and 78% immunopositivity in the co-treated groups respectively.The mean MT content in the precancer and the cancermodel was restored to near normal levels in all the three co-treated groups. Conclusion: These results suggest thatco-administration of aspirin, vitamin C or zinc resulted in a significant increase in MT mRNA gene expression, MTprotein expression and MT protein content which could possibly be one of the reasons for a chemo protective effectagainst progression to colonic cancer in a chemically induced DMH model in rat.Zinc supplement had a greater effecton metallothionein expression than aspirin or vitamin C.}, keywords = {Colon cancer,zinc,metallothionein,aspirin,Vitamin C}, url = {https://journal.waocp.org/article_73918.html}, eprint = {https://journal.waocp.org/article_73918_53545368578583163935745ed8bcec6c.pdf} }