@article { author = {Krishnankutty, Roopesh and Iskandarani, Ahmad and Therachiyil, Lubna and Uddin, Shahab and Azizi, Fouad and Kulinski, Michael and Bhat, Ajaz Ahmad and Mohammad, Ramzi M}, title = {Anticancer Activity of Camel Milk via Induction of Autophagic Death in Human Colorectal and Breast Cancer Cells}, journal = {Asian Pacific Journal of Cancer Prevention}, volume = {19}, number = {12}, pages = {3501-3509}, year = {2018}, publisher = {West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.}, issn = {1513-7368}, eissn = {2476-762X}, doi = {10.31557/APJCP.2018.19.12.3501}, abstract = {Background/ Objective: Camel milk is traditionally known for its human health benefits and believed to be a remedyfor various human ailments including cancer. The study was aimed to evaluate the inhibitory effects of commerciallyavailable camel milk on cancer cells and its underlying mechanism(s). Materials and Methods: Two cell lines:colorectal cancer HCT 116 and breast cancer MCF-7 were cultured with different doses of camel milk. The effects ofcamel milk on cell death were determined by MTT assay, viability by trypan blue exclusion assay and migration by invitro scratch assay. The mechanism was elucidated by western blotting and confocal microscopy was used to confirmautophagy. Results: Camel milk significantly reduced proliferation, viability as well as migration of both the cells.The accumulation of LC3-II protein along with reduction in expression of p62 and Atg 5-12, the autophagy proteinsimplied induction of autophagy. The (GFP)-LC3 puncta detected by confocal microscopy confirmed the autophagosomeformation in response to camel milk treatment. Conclusion: Camel milk exerted antiproliferative effects on humancolorectal HCT 116 and breast MCF-7 cancer cells by inducing autophagy.}, keywords = {colorectal cancer,breast cancer,Camel Milk,Autophagy}, url = {https://journal.waocp.org/article_80077.html}, eprint = {https://journal.waocp.org/article_80077_7e3e1b16ebe93b4c57f3778071e5a2d6.pdf} }