@article { author = {Taghibakhsh, Mehrdad and Farhadi, Sareh and Babaee, Afsaneh and Sheikhi, Maryam}, title = {The Effect of Hookah Use on Buccal Mucosa: Evaluation of Repair Index}, journal = {Asian Pacific Journal of Cancer Prevention}, volume = {20}, number = {4}, pages = {1109-1112}, year = {2019}, publisher = {West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.}, issn = {1513-7368}, eissn = {2476-762X}, doi = {10.31557/APJCP.2019.20.4.1109}, abstract = {Background and aim: Cigarettes, hookah, and tobacco are the most important etiologic factors for oral cancersand dysplastic lesions. This study was undertaken to determine the correlation between hookah use and the percentageof cells with micronucleus, karyorrhexis, karyolysis, and broken egg in the buccal mucosa; and secondly to comparehookah user and non-user in terms of repair index. Materials and methods: The present historical cohort study wascarried out on 72 samples taken from 36 hookah users and 36 control subjects. Smear samples were obtained fromparticipants’ buccal mucosa for cytological evaluation using Papanicolaou technique. Then, the percentages of cellswith micronucleus, karyorrhexis, karyolysis, and broken egg were recorded and the repair index was calculated. Datawere analyzed using Mann-Whitney U test. Results: A total of 72 samples taken from 36 hookah users and 36 controlsubjects were evaluated. The means of micronucleus scores in the buccal mucosa cells of hookah users and controlswere 10.7±2.6 and 5.8±2.0, the karyorrhexis scores in the hookah users and controls were 0.1±0.06 and 0.04±0.06,and the karyolysis scores in hookah users and controls were 0.16±0.05 and 0.08±0.06, respectively. These differenceswere statistically significant between hookah users and controls (P<0.001). The broken egg score was 0.66±0.07 for thehookah users and 0.03±0.04 for the control group, revealing a statistically significant difference (P<0.036). Finally, therepair index values were 0.03±0.01 and 0.05±0.13 in hookah users and controls, respectively. This difference was alsosignificant (P<0.026). Conclusion: The percentages of cells with micronucleus, karyorrhexis, karyolysis, and brokenegg in the buccal mucosa of hookah users were significantly higher than those in control group; in addition, the repairindex of the buccal mucosa cells in hookah users was significantly lower than that in the control group.}, keywords = {micronucleus,Repair index,buccal mucosa,hookah}, url = {https://journal.waocp.org/article_82377.html}, eprint = {https://journal.waocp.org/article_82377_de6a766da1c84ce36eee12cb7a741344.pdf} }