@article { author = {Ramezani, Mahnaz and Ramezani, Mahin and Darehkordi, Ali and Hassanshahi, Gholamhossein and Mirzaei, Mohammad Reza}, title = {The New Compound of (2R, 4S)-N-(2, 5-difluorophenyl)-4- Hydroxy-1-(2, 2, 2-Trifluoroacetyl) Pyrrolidine-2-Carboxamide to Mediate the Expression of Some Apoptosis Genes by the HepG2 Cell Line}, journal = {Asian Pacific Journal of Cancer Prevention}, volume = {20}, number = {5}, pages = {1457-1462}, year = {2019}, publisher = {West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.}, issn = {1513-7368}, eissn = {2476-762X}, doi = {10.31557/APJCP.2019.20.5.1457}, abstract = {Objectives: Hepatocellular carcinoma is one of the most frequent cancers worldwide, for the treatment of whichvarious therapy protocols and drugs have been introduced; however, none of them has suppressed cancer tissuescompletely. New research programs have been developed on cancer and the accompanied effects of novel synthesizedcompounds on cancer cell lines. Our latest reports on the molecular basis of cancer revealed a pattern of changes ingene expression triggered in the cancer pathway. Methods: HepG2 cell lines were cultured under similar conditionsin both test and control groups. The IC50 concentration of the (2R, 4S)-N-(2, 5-difluorophenyl)-4-hydroxy-1-(2, 2,2-trifluoroacetyl) pyrrolidine-2-carboxamide compound was used in the treatment group. After 48 hours from theculture, the expressional profiles of apoptosis pathway genes (84 genes) were studied using the PCR array method.Results: The findings demonstrated that the expression of some apoptosis-related genes pertaining to TNF, BCL2,IAP, and caspase families was regulated by (2R, 4S)-N-(2, 5-difluorophenyl)-4-Hydroxy-1-(2, 2, 2-Trifluoroacetyl)Pyrrolidine-2-Carboxamide. In the same vein, an alteration was observed in the expression of both pro-apoptotic andanti-apoptotic genes associated with the extrinsic and intrinsic apoptosis signaling pathways. Conclusions: Accordingto the data obtained, the pyrrolidine-2-carboxamide compound was demonstrated to be able to regulate the apoptoticactivities of HepG2 cells by affecting both pro-apoptotic and anti-apoptotic relevant genes.}, keywords = {pyrrolidine-2-carboxamide compound,HepG2 cells,apoptosis pathway}, url = {https://journal.waocp.org/article_84293.html}, eprint = {https://journal.waocp.org/article_84293_c1d19513fbcf27346876eb29ee157d4f.pdf} }