@article { author = {Auttajaroon, Jeerayuth and Chotivitayatarakorn, Peranart and Yamaoka, Yoshio and Vilaichone, Ratha-Korn}, title = {CYP2C19 Genotype, CagA Genotype and Antibiotic Resistant Strain of Helicobacter pylori Infection}, journal = {Asian Pacific Journal of Cancer Prevention}, volume = {20}, number = {4}, pages = {1243-1247}, year = {2019}, publisher = {West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.}, issn = {1513-7368}, eissn = {2476-762X}, doi = {10.31557/APJCP.2019.20.4.1243}, abstract = {Background: H. pylori is a class I carcinogen and major cause of gastric cancer. Few previous studies reportedrelationship between H. pylori infection, CYP2C19 genotype and functional dyspepsia (FD) subtype. The aim of thisstudy was to determine relationship between CYP2C19 genotype and FD subtype patients(host factor) with antibioticresistant strains of H. pylori infection and CagA genotype(bacterial factor). Methods: FD patients who were investigatedwith gastroscopy at Thammasat University Hospital, Thailand during March 2017-November 2017 were enrolled. Twoantral gastric biopsies were obtained for rapid urease test, E-test and cultures. CagA genotypes (CagA1a and CagA2a)were determined by PCR and CYP2C19 genotype was determined by PCR-RFLP. FD patients were categorized asepigastric pain syndrome(EPS) and postprandial distress syndrome (PDS). Results: 93 FD patients with H. pyloriinfection were enrolled (37 male, 56 female, mean age 54.5 years). There were 33 patients with EPS and 60 patientswith PDS. CYP2C19 genotype revealed 55.9% rapid metabolizer (RM), 40.9% intermediate metabolizer (IM) and3.2% poor metabolizer (PM) genotypes. Antibiotics susceptibility tests demonstrated 62.8% resistant to metronidazole,12.9% resistant to clarithromycin and 27.1% resistant to fluoroquinolone. CagA 1a and CagA 2a were demonstratedin 6 patients(11.5%) and 46 patients(88.5%). CagA2a genotype was more prevalent in PDS than EPS patients(94.3%vs.76.5%; P =0.08) without significance. In intermediate metabolizer (IM), CagA2a genotype was significanthigher in PDS than EPS(100% vs.25%; P=0.004). Conclusions: PDS, CYP2C19 RM genotype and CagA 2a gene ofH. pylori infection were the predominant type of FD in Thailand. Metronidazole remain the most common antibioticresistant strain of H. pylori infection in FD patients. PDS (host factor) was significantly related to CagA2a genotype(bacterial factors) only in patients with intermediate metabolizer. Appropriate dose of proton pump inhibitor (PPI) andcorrect regimens for H. pylori eradication in FD patients should be consider to improve clinical outcomes.}, keywords = {CYP2C19 genotype,antibiotic resistant strain,Helicobacter pylori}, url = {https://journal.waocp.org/article_85888.html}, eprint = {https://journal.waocp.org/article_85888_3d64fe381ea65d18e5169840cd450a34.pdf} }