@article { author = {Fatima, Naseem and Srivastava, Anand Narain and Nigam, Jaya and Raza, Syed Tasleem and Rizvi, Saliha and Siddiqui, Zainab and Kumar, Vijay}, title = {Low Expression of MicroRNA335-5p Is Associated with Malignant Behavior of Gallbladder Cancer: A Clinicopathological Study}, journal = {Asian Pacific Journal of Cancer Prevention}, volume = {20}, number = {6}, pages = {1895-1900}, year = {2019}, publisher = {West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.}, issn = {1513-7368}, eissn = {2476-762X}, doi = {10.31557/APJCP.2019.20.6.1895}, abstract = {Background: MicroRNAs (miRNAs) are non-coding RNAs that regulate multiple cellular processes during cancerprogression, identified to be involved in tumorgenesis of several cancers including cancers of digestive system. Howeverits role in gallbladder inflammatory disease (GID) and gallbladder cancer (GBC) has not been well documented.The present study was aimed to investigate the clinical significance of hsa-miRNA-335-5p (miR-335) in GBC andGID. Subjects and Methods: This prospective case control study, conducted from July 1, 2014 to December 1, 2017in Era’s Lucknow Medical College & Hospital, India, evaluated miR-335 expression by real-time polymerase chainreaction. Hundred tissue samples GID (control; n=50) and GBC (case; n=50) were studied. Relative quantification oftarget miR-335 expression was examined using the comparative cycle threshold method. Their expression was correlatedwith different clinicopathological parameters. Fishers’ exact test, Student’s t-test, and Chi-square test were used asappropriate for data analysis. Kaplan-Meier methods were used to calculate overall and disease-free survival rate.Two sided PGBC lesions when compared with GID lesions (P<0.001). The low expression level of miR-335 was correlated withhistological grade (P=0.007), clinical stage (P<0.001), lymph node metastasis (P<0.001) and liver metastasis (P=0.016).Reduced expression of miRNA-335 was associated with a shorter median overall survival (7 months vs. 25 months)in GBC patients (P<0.001). Conclusions: Down regulation of miR-335 is associated with the severity of the diseaseand thus indicate that miR-335 expression may serve as prognostic marker for GBC.}, keywords = {miRNA 335-5p,Real-Time PCR,Gallbladder inflammatory diseases,Gallbladder cancer,Prognosis}, url = {https://journal.waocp.org/article_88604.html}, eprint = {https://journal.waocp.org/article_88604_b1606167c20ee71a268f32229b8b509a.pdf} }