@article { author = {de Araújo, Renata Lázara and Savazzi, Suiane and Fujimori, Mahmi and Deluque, Alessandra and Honório-França, Eduardo Luzia and Pertuzatti Konda, Paula Becker and Honório-França, Adenilda Cristina}, title = {Effects of Mangaba (Hancornia speciosa) Fruit Extract Adsorbed onto PEG Microspheres in MCF-7 Breast Cancer Cells Co-Cultured with Blood Cells}, journal = {Asian Pacific Journal of Cancer Prevention}, volume = {20}, number = {7}, pages = {1995-2001}, year = {2019}, publisher = {West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.}, issn = {1513-7368}, eissn = {2476-762X}, doi = {10.31557/APJCP.2019.20.7.1995}, abstract = {Objective: To evaluate the antitumor effects of polyethylene glycol (PEG) microspheres with adsorbed Hancorniaspeciosa ethanolic extract (HSEE) on blood mononuclear (MN) cells co-cultured with MCF-7 breast cancer cells.Methods: PEG microspheres were adsorbed with HSEE and examined by flow cytometry and fluorescence microscopy.MCF-7 and MN cells obtained from volunteer donors were pre-incubated alone or co-cultured (MN and MCF-7cells) for 24 h with or without HSEE, PEG microspheres or PEG adsorbed with HSEE (PEG-HSEE). Cell viability,superoxide release and superoxide dismutase were determined. Results: Fluorescence microscopy showed that PEGmicrospheres were able to absorb HSEE throughout their surface. Irrespective of the treatment, the viability index ofMN cells, MCF-7 and their co-culture was not affected. Superoxide release increased in co-cultured cells treated withHSEE, adsorbed or not onto PEG microspheres. In co-cultured cells, SOD levels in culture supernatant increased inthe treatment with HSEE, adsorbed onto PEG microspheres or not. Conclusion: HSEE has direct effects on MN cellsco-cultured with MCF-7 cells. The results suggest the benefits of Hancornia speciosa fruit consumption by women atrisk of breast cancer. In addition, because PEG-HSEE maintained oxidative balance in co-cultured cells, it is a promisingalternative for the treatment of tumor cells.}, keywords = {Microspheres,Antioxidant activity,Brazilian fruit,Biomaterial,cancer}, url = {https://journal.waocp.org/article_88637.html}, eprint = {https://journal.waocp.org/article_88637_9e2716c97d35034ec1a29127f0d732f6.pdf} }