@article { author = {Ghazanfari, Tayebeh and Asaadi Tehrani, Golnaz and Maziri, Parisa}, title = {The Relationship between the Methylation of Promoter Regions of Tumor Suppressor Genes PTEN and APC with Endometrial Cancer}, journal = {Asian Pacific Journal of Cancer Prevention}, volume = {20}, number = {8}, pages = {2259-2265}, year = {2019}, publisher = {West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.}, issn = {1513-7368}, eissn = {2476-762X}, doi = {10.31557/APJCP.2019.20.8.2259}, abstract = {Background: Endometrial neoplasms is one of the most typical gynecologic diseases with harmful effects. Promoterhypermethylation is an important mechanism of the inactivation of tumor suppressor genes in endometrial neoplasms.Epigenetic changes of the PTEN and APC genes have shown to be present in various cancers. Therefore, in this study,we have investigated the association between the promoter hypermethylation of PTEN and APC genes with endometrialneoplasms. Methods: For this study, 28 patients with endometrial neoplasms as well as 22 controls were studied.Analysis of the promoter methylation regions of PTEN and APC genes were performed by Methylation-Specific PCR.Results: The frequency of PTEN and APC genes promoter methylation was 28.57% and 17.86% in tumor tissues, and11.54% and 3.85% in blood samples, respectively. We found a significant relationship between blood and tissue inPTEN methylation (p = 0.0353). Additionally, we determined a closely significant difference between normal tissueand tumor tissue of the PTEN gene (p = 0.0787) and blood and tissue samples of the APC gene in methylated promoterregions (p=0.0623). Furthermore, these results suggest that there is no significant relationship between the promotermethylation of PTEN and APC with clinical characteristics. Conclusion: DNA methylation deficiency is a well knownhighlighted factor in tumorigenesis, therefore the promoter hypermethylation of PTEN and APC can be indicated as arisk factor in endometrial neoplasms.}, keywords = {Endometrial neoplasms,DNA Methylation,APC,PTEN}, url = {https://journal.waocp.org/article_88669.html}, eprint = {https://journal.waocp.org/article_88669_4e7475b681278ad4c49da6d512247c35.pdf} }