@article { author = {Ahmadi, Mohadeseh and Hedayatizadeh-Omran, Akbar and Alizadeh-Navaei, Reza and Saeedi, Majid and Zaboli, Ehsan and Amjadi, Omolbanin and Kelidari, Hamidreza and Besharat, Zahra}, title = {Effects of Vitamin E on Doxorubicin Cytotoxicity in Human Breast Cancer Cells in Vitro}, journal = {Asian Pacific Journal of Cancer Prevention}, volume = {23}, number = {1}, pages = {201-205}, year = {2022}, publisher = {West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.}, issn = {1513-7368}, eissn = {2476-762X}, doi = {10.31557/APJCP.2022.23.1.201}, abstract = {Objective: This study aimed to evaluate in vitro synergistic anticancer effect of doxorubicin combined with Vitamin E. Methods: The MTT assay was utilized to assess the cytotoxicity of Vitamin E and vitamin E combined with doxorubicin and vital activities of SKBR3, MDA-MB-231, and HFF cells over a 24-hour incubation period. In addition, the antioxidant properties of these interventions and the decrease of reactive oxygen species (ROS) content caused by the treatment were evaluated. Results: The antiproliferative effect of doxorubicin increased significantly in combination with vitamin E (Doxcorobicin 2µM vs. Vitamin E 120µM, P=0.000). Despite reducing cell ROS content due to vitamin E treatment, the combination of vitamin E and doxorubicin showed no significant synergistic effect (Doxcorobicin 2µM vs. Vitamin E 120µM, P=0.998). Conclusion: This study indicated that the doxorubicin–vitamin E treatment reduced the viability of breast cancer cells with the minimum side effects on normal cells. In addition, the high dosage of vitamin E intensified the cytotoxicity of doxorubicin.}, keywords = {Antioxidant,breast cancer,doxorubicin,Vitamin E}, url = {https://journal.waocp.org/article_89930.html}, eprint = {https://journal.waocp.org/article_89930_fe46da6d4c1b645a1c84b67c8e1e38ea.pdf} }