@article { author = {Al Balushi, Najwa and Hassan, Syed and Abdullah, Nada and Al Dhahli, Buthaina and Al Bahlani, Shadia and Ahmed, Ikhlas and Tsang, Benjamin and Dobretsov, Sergey and Tamimi, Yahya and Burney, Ikram A}, title = {Addition of Gallic Acid Overcomes Resistance to Cisplatin in Ovarian Cancer Cell Lines}, journal = {Asian Pacific Journal of Cancer Prevention}, volume = {23}, number = {8}, pages = {2661-2669}, year = {2022}, publisher = {West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.}, issn = {1513-7368}, eissn = {2476-762X}, doi = {10.31557/APJCP.2022.23.8.2661}, abstract = {Objective: Ovarian cancer is one of the leading causes of cancer-related mortality in women, and is often associated with drug resistance. Therefore, finding effective drugs, including naturally derived compounds, is urgently needed. Herein, we aimed to test the anti-cancer potential of gallic acid monohydrate (GA) and its congeners on cisplatin-sensitive (A2780S), and resistant (A2780CP) ovarian cancer and normal ovarian (HOSE6-3) cell lines. Methods: Cytotoxicity was assessed by AlamarBlue and CCK08 assays by exposing cells to different concentrations of cisplatin (0-21µg/mL), GA and its congeners (0-100µg/mL), and a combination of GA and cisplatin. Apoptosis was estimated by Hoechst stain and monitoring the relative RNA expression of the apoptotic effector caspase-3 using qRT-PCR. Results: GA decreased cell viability in a concentration-dependent manner in all cell lines, with an IC50 of 19.39µg/mL (A2780S), 35.59 µg/mL (A2780CP), and 49.32µg/mL (HOSE6-3). GA displayed higher cytotoxicity than its congeners. An apoptotic rate estimation of approximately 20% and 30% was obtained in A2780S and A2780CP. While the cytotoxicity observed with cisplatin and GA was comparable, combining the two enhanced the cytotoxicity significantly, especially in the A2780CP cell line (p<0.05). Conclusion: These data suggest that GA may help overcome the resistance. Hence, the cytotoxic effects of GA, especially on chemo-resistant ovarian cancer cells merit further investigation. }, keywords = {Ovarian Cancer,Gallic acid,Cisplatin,Cytotoxicity,Chemo-resistance}, url = {https://journal.waocp.org/article_90248.html}, eprint = {https://journal.waocp.org/article_90248_f16eb118cc43f608aac911b54cd13041.pdf} }