eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3307
3316
10.31557/APJCP.2018.19.12.3307
80036
Induction of Apoptotic Death and Cell Cycle Arrest in HeLa Cells by Extracellular Factors of Breast Cancer Cells
Devashree Jahagirdar
devashreejahagirdar@gmail.com
1
Charusheela R Gore
shantugore@gmail.com
2
Himadri Patel
patelhimadri2@gmail.com
3
Kunjal Maria
mariakunjal@gmail.com
4
Ishita Tnadon
tandoanishita11@gmail.com
5
Nilesh K Sharma
nilesh.sharma@dpu.edu.in
6
Cancer and Translational Research Lab, Dr D.Y. Patil Biotechnology and Bioinformatics Institute, Dr D.Y. Patil Vidyapeeth, India.
Department of Pathology, Dr. D. Y. Patil Medical College, Hospital and Research Centre, Pimpri, Pune, Maharashtra, India.
Cancer and Translational Research Lab, Dr D.Y. Patil Biotechnology and Bioinformatics Institute, Dr D.Y. Patil Vidyapeeth, India.
Department of Pathology, Dr. D. Y. Patil Medical College, Hospital and Research Centre, Pimpri, Pune, Maharashtra, India.
Cancer and Translational Research Lab, Dr D.Y. Patil Biotechnology and Bioinformatics Institute, Dr D.Y. Patil Vidyapeeth, India.
Cancer and Translational Research Lab, Dr D.Y. Patil Biotechnology and Bioinformatics Institute, Dr D.Y. Patil Vidyapeeth, India.
Background: There are evidences on the role of extracellular factors in cellular communication between cancercells and non-cancerous cells to support tumor progression and a phenomenon of cancer cachexia. However, evidencesare scarce to show the effects of extracellular factors from one carcinoma microenvironment upon growth and survivalof another carcinoma. Methodology: To address the above issue, we have selected excised breast carcinoma tissuesamples and in vitro grown MCF-7 sources of extracellular factors and tested their effects to evaluate growth andproliferation inhibitory potential against a cervical carcinoma cell line HeLa. Results: Data from the in vitro experimentslike Trypan blue dye exclusion, MTT assay, cell cycle assay and annexin V/PI staining lead us to suggest that theextracellular factors collected from the culture medium of in vitro grown MCF-7 and excised breast carcinoma tissueplay an apoptosis inducing and cell cycle arrest role in HeLa. In these in vitro experiments, we detected the presence ofup to 40-50% apoptotic cell death in HeLa cells and increase in G2-M cell cycle phase from 11%-25% due to treatmentwith extracellular factors from human breast carcinoma cells. Discussion and Conclusion: These observations arenovel and suggest that extracellular factors from breast carcinoma play an apoptosis inducing and growth inhibitoryrole upon on HeLa cells. This study can also support the concept of cancer cachexia and a possible hypothesis for rarechance of synchronous two or more primary tumor in a single patient.
https://journal.waocp.org/article_80036_c79138282757caf0db97da13b57bcce9.pdf
heterogeneity
growth
Death
Neoplasms
microenvironment
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3317
3320
10.31557/APJCP.2018.19.12.3317
80040
Prevalence of BCR-ABL T315I Mutation in Malaysian Patients with Imatinib-Resistant Chronic Myeloid Leukemia
Yuslina Mat Yusoff
yuslina@imr.gov.my
1
Zahidah Abu Seman
zahidah@imr.gov.my
2
Norodiyah Othman
norodiyah@imr.gov.my
3
Nor Rizan Kamaluddin
norizan@imr.gov.my
4
Ezalia Esa
ezalia@imr.gov.my
5
Nor Amalina Zulkiply
noramalinazulkiply@gmail.com
6
Julia Abdullah
leeya987@yahoo.com
7
Zubaidah Zakaria
zubaidah@imr.gov.my
8
Haematology Unit, Cancer Research Centre, Institute for Medical Research, Jalan Pahang, Wilayah Persekutuan Kuala Lumpur, Malaysia.
Haematology Unit, Cancer Research Centre, Institute for Medical Research, Jalan Pahang, Wilayah Persekutuan Kuala Lumpur, Malaysia.
Haematology Unit, Cancer Research Centre, Institute for Medical Research, Jalan Pahang, Wilayah Persekutuan Kuala Lumpur, Malaysia.
Haematology Unit, Cancer Research Centre, Institute for Medical Research, Jalan Pahang, Wilayah Persekutuan Kuala Lumpur, Malaysia.
Haematology Unit, Cancer Research Centre, Institute for Medical Research, Jalan Pahang, Wilayah Persekutuan Kuala Lumpur, Malaysia.
Haematology Unit, Cancer Research Centre, Institute for Medical Research, Jalan Pahang, Wilayah Persekutuan Kuala Lumpur, Malaysia.
Haematology Unit, Cancer Research Centre, Institute for Medical Research, Jalan Pahang, Wilayah Persekutuan Kuala Lumpur, Malaysia.
Haematology Unit, Cancer Research Centre, Institute for Medical Research, Jalan Pahang, Wilayah Persekutuan Kuala Lumpur, Malaysia.
Objective: Chronic Myeloid Leukemia (CML) is caused by a reciprocal translocation between chromosomes 9and 22, t(9;22) (q34;q11) which encodes for the BCR-ABL fusion protein. Discovery of Imatinib Mesylate (IM) asfirst line therapy has brought tremendous improvement in the management of CML. However, emergence of pointmutations within the BCR-ABL gene particularly T315I mutation, affects a common BCR-ABL kinase contact residuewhich impairs drug binding thus contribute to treatment resistance. This study aims to investigate the BCR-ABL T315Imutation in Malaysian patients with CML. Methods: A total of 285 patients diagnosed with CML were included in thisstudy. Mutation detection was performed using qualitative real-time PCR (qPCR). Results: Fifteen out of 285 samples(5.26%) were positive for T315I mutations after amplification with real-time PCR assay. From the total number ofpositive samples, six patients were in accelerated phase (AP), four in chronic phase (CP) and five in blast crisis (BC).Conclusion: Mutation testing is recommended for choosing various tyrosine kinase inhibitors (TKIs) to optimizeoutcomes for both cases of treatment failure or suboptimal response to imatinib. Therefore, detection of T315I mutationin CML patients are clinically useful in the selection of appropriate treatment strategies to prevent disease progression.
https://journal.waocp.org/article_80040_002f76a33f14d1cd9e6d81d751beccb8.pdf
Chronic myeloid leukemia
BCR/ABL gene
T315I mutation
tyrosine kinase inhibitor
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3321
3334
10.31557/APJCP.2018.19.12.3321
77046
Simulation Models in Gastric Cancer Screening: A Systematic Review
Ayako Matsuda
amatsuda@med.teikyo-u.ac.jp
1
Kumiko Saika
ksaika@ncc.go.jp
2
Rina Tanaka
r-tana@hirosaki-u.ac.jp
3
Yuri Ito
itou-yu2@mc.pref.osaka.jp
4
Keisuke Fukui
fukui-ke@mc.pref.osaka.jp
5
Ken-Ichi Kamo
kamo@sapmed.ac.jp
6
Department of Hygiene and Public Health, Teikyo University School of Medicine, Tokyo, Japan.
Division of Screening Assessment and Management, Center for Public Health Sciences, National Cancer Center, Tokyo, Japan.
Department of Medical Informatics, Hirosaki University Graduate School of Medicine, Hirosaki, Aomori, Japan.
Department of Cancer Epidemiology, Cancer Control Center, Osaka International Cancer Institute, Osaka, Japan.
Department of Cancer Epidemiology, Cancer Control Center, Osaka International Cancer Institute, Osaka, Japan.
Department of Plastic and Reconstructive Surgery, Sapporo Medical University School of Medicine, Chuo-ku, Sapporo, Japan.
Background: Together with such high-quality approaches as randomized controlled trials and large-scale cohortstudies, simulation models are often employed to evaluate the effect of cancer screening methods and decide ontheir appropriateness. This study aimed to evaluate all effects of gastric cancer screening that have been assessedusing simulation models, including cost-effectiveness, mortality reduction, and early-stage detection. Methods: Weperformed a systematic review using PubMed and Web of Science. We evaluated the effect of screening related tocost, such as incremental cost-effectiveness and incremental cost-effectiveness ratios; we also separately assessedeffects other than cost, such as quality-adjusted life-years, number of deaths prevented, life-years saved, relative riskof mortality from gastric cancer, life expectancy, and incidence reduction. The methods targeted for evaluation wereHelicobacter pylori testing or endoscopy. Results: We identified 19 studies dealing with simulation models in gastriccancer screenings: 14 examined H. pylori screening and 7 focused on endoscopy. Among those studies, two assessedboth H. pylori and endoscopy screening. Most of the studies adopted a Markov model, and all the studies evaluatedcost-effectiveness. Of the 14 H. pylori screening studies, 13 demonstrated cost-effectiveness and 11 also showed goodresults other than cost-effectiveness, such as extension of life-years and increase in early-stage detection. In three of thefive endoscopy studies, the target population was patients; all five studies obtained good results for cost-effectivenessand four observed good results other than for cost-effectiveness. Conclusions: In this study, we showed that the H.pylori screening test was cost-effective in terms of simulation model investigations. However, the H. pylori screeningtest should not ordinarily be recommended since there is insufficient evidence that it reduces gastric cancer mortality.In Japan, simulation modeling should be employed to plan for cancer control, and the appropriate use of simulationmodels should be examined for future use.
https://journal.waocp.org/article_77046_16e6f0bc29f5539dcfee492984d4ed65.pdf
Simulation Model
Gastric cancer
Screening
Systematic review
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3335
3339
10.31557/APJCP.2018.19.12.3335
77412
An Effective Two Way Classification of Breast Cancer Images: A Detailed Review
Sinthia P
sinthiapanneerselvam@gmail.com
1
Malathi M
malathi_friends2000@yahoo.co.in
2
Department of EIE, Saveetha Engineering College, Chennai, India.
Department of EIE, Saveetha Engineering College, Chennai, India.
Cancer, a disease of cells, causes cell growth which differs from normal cell growth ratio, this cell growth spreadsin the human body and kills the body cells. Breast cancer, it’s a highly heterogeneous disease and western womencommonly witness this. Mammography, a pre-screening X-ray based check is used to diagnose woman’s breast cancer.This basic test mode helps in identifying breast cancer at early stage and this early stage detection would support inrecovering more number of women from this serious disease. Medical centres deputed highly skilled radiologists andthey were given the responsibility of analysing this mammography results but still human errors are inevitable. An errorfrequency ratio is high when radiologists exhausted in their analysis task and leads variations in either observationsie., internal or external observation. Also, quality of the image plays vital role in Mammographic sensitivity and leadsto variation. Several automation processes were tried in streamlining and standardising diagnosis analysis process andquality of breast cancer images were improved. This paper inducts a two way mode algorithm for grouping of breastcancer images to 1. benign (tumour growing, but not dangerous) and 2. malignant (cannot be controlled, it causes death)classes. Two-way mode data mining algorithms are used due to thinly dispersed distribution of abnormal mammograms.First type algorithm is k-means algorithm, which regroups the given data elements into clusters (ie., prioritized by theusers). Second type algorithm is Support Vector Machine (SVM), which is used to identify the most suitable functionwhich differentiates the members based on the training data.
https://journal.waocp.org/article_77412_8fbcb6fede4e15e68460e2291f7e2ef8.pdf
Mammogram
breast cancer
k-means
SVM
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3341
3351
10.31557/APJCP.2018.19.12.3341
80052
Overview on Epigenetic Re-programming: A Potential Therapeutic Intervention in Triple Negative Breast Cancers
Ezanee Azlina Mohamad Hanif
ezanee.azlina.mohamad.hanif@ppukm.ukm.edu.my
1
Shamsul Azhar Shah
drsham@ppukm.ukm.edu.my
2
UKM Medical Molecular Biology Institute (UMBI), University Kebangsaan Malaysia Medical Centre, Jalan Yaccob Latiff, Bandar Tun Razak, Cheras, Kuala Lumpur, Malaysia.
UKM Medical Molecular Biology Institute (UMBI), University Kebangsaan Malaysia Medical Centre, Jalan Yaccob Latiff, Bandar Tun Razak, Cheras, Kuala Lumpur, Malaysia.
Breast cancer treatments leads to variable responses. Hormonal therapy is beneficial to receptor positive breast cancersubtypes and display better clinical outcome than triple negative breast cancers (TNBCs) with FEC (5-Fluorouracil,Epirubicin and Cyclophosphamide) the mainstay chemotherapy regiment. Owning to their negative expressions ofestrogen (ER), progesterone (PR) and HER2 receptors, disease recurrence and metastasis befalls some patients indicatingresistance to FEC. Involvement of epigenetic silencing through DNA methylation, histone methylation, acetylation andsumoylation may be the key player in FEC chemoresistance. Epigenetic and molecular profiling successfully classifiedbreast cancer subtypes, indicating potential driver mechanisms to the progression of TNBCs but functional mechanismsbehind chemoresistance of these molecular markers are not well defined. Several epigenetic inhibitors and drugs havebeen used in the management of cancers but these attempts are mainly beneficial in hematopoietic cancers and notspecifically favourable in solid tumours. Hypothetically, upon administration of epigenetic drugs, recovery of tumoursuppressor genes is expected. However, high tendency of switching on global metastatic genes is predicted. Polycombrepressive complex (PRC) such as EZH2, SETD1A, DNMT, is known to have repressive effects in gene regulation andshown to inhibit cell proliferation and invasion in breast cancers. Individual epigenetic regulators may be an optionto improve chemo-drug delivery in cancers. This review discussed on molecular signatures of various breast cancersubtypes and on-going attempts in understanding underlying molecular mechanisms of epigenetic regulators as wellas providing insights on possible ways to utilize epigenetic enzymes/inhibitors with responses to chemotherapeuticdrugs to re-program cellular and biological outcome in TNBCs.
https://journal.waocp.org/article_80052_4f475799e37b2b67d0a0caafbf0c2317.pdf
triple negative breast cancers
TNBCs
epigenetic modifiers
FEC
Relapse
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3353
3359
10.31557/APJCP.2018.19.12.3353
77417
Association of IL-10 rs1800871 and rs1800872 Polymorphisms with Breast Cancer Risk: A Systematic Review and Meta-Analysis
Mansour Moghimi
moghimim1350@gmail.com
1
Hossein Ahrar
ahrar.hossein43@gmail.com
2
Mojgan Karimi-Zarchi
mk.zarchi55@gmail.com
3
Kazem Aghili
kaz.aghili@gmail.com
4
Marjansadat Salari
sm.latifi65@gmail.com
5
Masoud Zare-Shehneh
masoud.zareshehneh@gmail.com
6
Hossein Neamatzadeh
neamatzadehh@gmail.com
7
Department of Pathology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Department of Radiology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Department of Gynecology and Obstetrics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Department of Radiology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Department of Biology, Ashkezar Branch, Islamic Azad University, Yazd, Iran.
Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Background: The rs1800871 and rs1800872 polymorphisms of interleukin 10 (IL-10) gene has been indicated tobe associated with breast cancer (BC) risk, but study results are still debatable. To derive a more precise evaluation, weperformed a comprehensive meta-analysis. Methods: Multiple electronic databases were searched to identify studiesassessing the IL-10 rs1800871 and rs1800872 polymorphisms with BC risk. Results: A total of 21 case-control studieswith 6054 cases and 6355 controls were included in this met-analysis. There was a significant association between thers1800871 polymorphism and BC risk (CT vs. TT: OR= 1.17, 95% CI 1.01-1.35, p=0.02; and CC+CT vs. TT: OR= 1.29,95% CI 1.00-1.66, p=0.04). Moreover, increased BC risks were also associated with the rs1800872 polymorphism (Cvs. A: OR= 1.29, 95% CI 1.04-1.60, p=0.01; CC vs. AA: OR= 1.54, 95% CI 1.03-2.30, p=0.03; CC+CA vs. AA: OR=1.43, 95% CI 1.01-2.01, p=0.03; and CC vs. CA+AA: OR= 1.23, 95% CI 1.01-1.51, p=0.04). A pooling of the studieswas also conducted by ethnicity, but failed to show an association of IL-10 rs1800871 and rs1800872 polymorphismwith BC risk in Asians and Caucasians. Conclusions: Our results are inconsistent with previous meta-analysis suggeststhat IL-10 rs1800871 and rs1800872 polymorphisms might contribute to BC susceptibility in overall population, butnot by ethnicity.
https://journal.waocp.org/article_77417_52a43dd5a140e41f1052c6e419b48ddf.pdf
breast cancer
Interleukin-10
Polymorphism
Meta-analysis
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3361
3366
10.31557/APJCP.2018.19.12.3361
77047
Phylogeny and Classification of Human Papillomavirus (HPV)16 and HPV18 Variants Based on E6 and L1 genes in Tunisian Women with Cervical Lesions
Mouna Jendoubi-Ferchichi
mounaferchichi@hotmail.fr
1
Latifa Satouri
latifasatouri@hotmail.fr
2
Fatma Ghoul
fatmaghoul80@gmail.com
3
Monia Malek-Mellouli
melloulimonia@yahoo.fr
4
Abdel Moneem Derbal
derbal.abdelmonem@topnet.tn
5
Mohamed Kamel Makni
mknimedkamel@topnet.tn
6
Hedi Reziga
hedireziga@rns.tn
7
Ali Baba
dralibaba@live.fr
8
Mohamed Zili
mohamed.zili@rns.tn
9
Michel Segondy
m-segondy@chu-montpellier.fr
10
Ridha Khelifa
khelifa.ridha@rns.com
11
Viral and Molecular Tumor Diagnostics Unit, Laboratory Services, Habib Thameur Hospital, Tunis, Tunisia.
Viral and Molecular Tumor Diagnostics Unit, Laboratory Services, Habib Thameur Hospital, Tunis, Tunisia.
Viral and Molecular Tumor Diagnostics Unit, Laboratory Services, Habib Thameur Hospital, Tunis, Tunisia.
Department of Gynecology and Obstetrics, La Rabta Maternity and Neonatology Center, Tunis, Tunisia.
Laboratory of Pathological Anatomy, Kheireddine Pacha Avenue, Tunis, Tunisia.
Laboratory of Pathological Anatomy, Kheireddine Pacha Avenue, Tunis, Tunisia.
Department of Gynecology and Obstetrics, La Rabta Maternity and Neonatology Center, Tunis, Tunisia.
Gynecology and Obstetrics office, 25 Habib Bourguiba Avenue, Tunis, Tunisia.
Viral and Molecular Tumor Diagnostics Unit, Laboratory Services, Habib Thameur Hospital, Tunis, Tunisia.
Pathogenesis and Control of Chronic Infections, INSERM, University of Montpellier, EFS, CHU Montpellier, Montpellier, France.
Viral and Molecular Tumor Diagnostics Unit, Laboratory Services, Habib Thameur Hospital, Tunis, Tunisia.
Background: High-risk human papillomavirus (HPV) types are the main etiological factors for cervical cancer.HPV16 and HPV18 are generally the most common forms associated with development of high-grade cervical lesions.This study was undertaken to identify intratypic variants of HPV16 and HPV18 among women with cervical lesionsin Tunisia. Materials and Methods: DNA was extracted from cervical samples collected from 49 women. using aPureLinkTM Genomic DNA mini Kit (Invitrogen). E6 and L1 open reading frames (ORF) were amplified by PCRand viral DNA amplicons were subjected to automated sequencing using Big Dye Terminators technology (AppliedBiosystems). The obtained sequences were analyzed using an appropriate software program to allow phylogenetictrees to be generated. Results: HPV16 and HPV18 were detected in 15 and 5 cases, respectively. HPV16 E6 sequencesclustered with the European German lineage (A2) whereas one isolate diverged differently in the L1 region andclustered with the African sub-lineage (B1). HPV 18 E6 sequences clustered with the European sub-lineage (A1)but L1 sequences clustered as a new clade which diverged from A1-A5. Conclusions: Our results suggest that thedistribution of HPV16 and HPV18 sequences in women with cervical lesions in Tunisia is mainly related to Europeanepidemiological conditions and point to the presence of recombinant HPV forms.
https://journal.waocp.org/article_77047_1f98a903308d4ca5c9ac60068ecfd6c7.pdf
HPV16
HPV18
Phylogeny
Tunisia
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3367
3374
10.31557/APJCP.2018.19.12.3367
77403
Factors Influencing Knowledge and Practice Regarding Cervical Cancer and Pap smear Testing among Omani Women
Nasar Alwahaibi
nasar@squ.edu.om
1
Waad Alsalami
promisesalami94@gmail.com
2
Nasra Alramadhani
u1014@squ.edu.om
3
Atheer Alzaabi
atheeralzab@gmail.com
4
Department of Allied Health Sciences, College of Medicine and Health Sciences, Sultan Qaboos University, Sultanate of Oman.
Department of Allied Health Sciences, College of Medicine and Health Sciences, Sultan Qaboos University, Sultanate of Oman.
Department of Allied Health Sciences, College of Medicine and Health Sciences, Sultan Qaboos University, Sultanate of Oman.
Department of Allied Health Sciences, College of Medicine and Health Sciences, Sultan Qaboos University, Sultanate of Oman.
Background: Knowledge of cervical cancer and performance of Pap smear testing are influenced by severalsociodemographic factors. This study aimed to describe the effect of relevant variables on knowledge and compliancewith guidelines in Oman. Methods: In this cross-sectional survey, participants were divided into three groups: patientswho attended Outpatient Gynecology Department, female medical staff and university graduate students. Results: Therewere 204 outpatients, 133 staff, and 157 students. Adequate knowledge among was seen in 38.7%, 35.3%, and 7.6%,respectively. Knowledge of cervical cancer and Pap smear was significantly lower among outpatients with secondaryeducation, while those with high level of income were more likely to have adequate cancer knowledge. Uptake of Papsmear was significantly greater among outpatients aged ≥ 30 years, with high income and a positive history of cancer.Conclusion: Culturally tailored interventions that focus on improving cancer risk knowledge are needed to maximizescreening uptake for cervical cancer.
https://journal.waocp.org/article_77403_04b76b58653f09f4ea1684089a9fe4b8.pdf
Cervical cancer
Pap smear
Knowledge
performance
sociodemographic factors
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3375
3381
10.31557/APJCP.2018.19.12.3375
80094
Combining the Prostate Cancer Risk Index (PRIX) with the Presence of Secondary Circulating Prostate Cells to Predict the Risk of Biochemical Failure after Radical Prostatectomy for Prostate Cancer
Nigel P Murray
nigelpetermurray@gmail.com
1
Socrates Aedo
socrates.aedo@gmail.com
2
Cynthia Fuentealba
cynthia_fuentealba_s@yahoo.es
3
Eduardo Reyes
ereyessan@gmail.com
4
Omar Jacob
mimilizama@gmail.com
5
CTC Unit, Faculty of Medicine, University Finis Terrae, Chile.
CTC Unit, Faculty of Medicine, University Finis Terrae, Chile.
Urology Service, Hospital de Carabineros de Chile, Santiago, Chile.
Faculty of Medicine University Diego Portales Santiago, Chile.
Urology Service, Hospital de Carabineros de Chile, Santiago, Chile.
Introduction: The use of pre- and post-surgery variables has been used to create nomograms in order to identifypatients at high risk of treatment failure. The PRIX nomogram is one such device; we compare the PRIX nomogramwith the presence of secondary circulating prostate cells to predict those men who will undergo treatment failure.Methods and Patients: Men who underwent radical prostatectomy for prostate cancer entered the study. The PRIXscore was calculated from the total serum PSA pre-surgery, the biopsy Gleason score and clinical stage. Circulatingprostate cells were detected from venous blood one month after surgery, using differential gel centrifugation and standardimmunocytochemistry with anti-PSA. A test was considered positive when 1 CPC/blood sample was detected. Patientswere followed up for five years and biochemical failure was defined as a serum PSA >0.2ng/ml. Kaplan-Meier andCox proportional models were used to calculate survival curves. Results: 321 men participated, of whom 131 (40.8%)underwent biochemical failure within 5 years. A higher PRIX score was associated with increased failure risk, as wasthe presence of CPCs. The predictive power of CPCs was significantly higher than the PRIX score. Combining thetwo methods, for equal PRIX scores, scores but CPC positive had a worse biochemical failure free survival than menwith high PRIX scores but CPC negative. For men with PRIX scores of ≥4 the use of CPC detection did not aid in theclinical decision making process. For those with PRIX scores of 0 and 1, CPC detection identified men with a high riskof treatment failure. Conclusions: The combined PRIX/CPC score improved the predictive values of men at high riskof biochemical failure. Both are simple systems that could be incorporated in a general hospital. Further multicenterstudies are warranted to confirm these results.
https://journal.waocp.org/article_80094_a40d2d4a5890fa6e59ef5bded70d6ec1.pdf
Prostate Cancer
PRIX score
circulating prostate cells
biochemical failure
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3383
3392
10.31557/APJCP.2018.19.12.3383
77404
CD200 Expression in Diagnostic and Prognostic Assessment of Mature B Cell Lymphophoproliferative Neoplasms
Neveen Bahaa El Din Fouad
nbfpiano@yahoo.com
1
Noha Yehia Ibrahim
dr.noha11@hotmail.com
2
Rania Salah Abdel Aziz
rania_smansy@yahoo.com
3
Sarah Khalid Ibrahim
sarahibrahim_2020@yahoo.com
4
Clinical Pathology Department, Faulty of Medicine, Cairo University, Egypt.
Clinical Oncology Department (NEMROCK), Cairo University, Egypt.
Clinical Pathology Department, National Cancer Institute (NCI), Cairo University, Egypt.
Clinical Pathology Department, National Cancer Institute (NCI), Cairo University, Egypt.
Background: Multiparameter flow cytometry is a useful tool for diagnostic evaluation of mature B-cell neoplasms(MBN). Recently, it has been shown that assessment of CD200 expression may improve the distinction betweenchronic lymphocytic leukemia (CLL; CD200 positive) and mantle cell lymphoma (MCL; CD200 negative), but anypotential as a prognostic marker for CLL remains to be established. Materials and methods: This cross sectionalstudy was conducted on sixty-seven patients newly diagnosed as having mature B-cell lymphoproliferative disordersLevels of CD 200 in lymphoma cells were assessed. Results: CD200 was consistently expressed in CLL and hairycell leukemia B cells, but not in MCL cells. Heterogeneous expression was noted in other CD5 positive Non-Hodgkinlymphomas. High CD200 expression (≥50%) was associated with a higher CD5, 19 and CD23 expression, olderage, higher TLC and absolute lymphocyte count, hepatomegaly, splenomegaly and a higher Rai stage. There wereno significant correlations between CD200 expression and response to treatment. Conclusion: CD200 could be of highvalue in distinguishing CLL, MCL, and atypical CLL. CD200 expression can also be of prognostic and therapeuticvalue in CLL cases.
https://journal.waocp.org/article_77404_5f3dea8c8f9e1e7e7776e6a8296f94bb.pdf
CD200
Mature B-cell neoplasms
Flowcytometry
CLL
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3393
3400
10.31557/APJCP.2018.19.12.3393
77405
Cytotoxic and Antiproliferative Activity of Polyisoprenoids in Seventeen Mangroves Species Against WiDr Colon Cancer Cells
Dini P Sari
permatasaridini21@yahoo.com
1
Mohammad Basyuni
m.basyuni@usu.ac.id
2
Poppy AZ Hasibuan
poppyanjelisa94@gmail.com
3
Sumardi Sumardi
mardisaad@gmail.com
4
Arif Nuryawan
arif.nury@gmail.com
5
Ridha Wati
ridhawati62@gmail.com
6
Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan 20155, Indonesia.
Department of Forestry, Faculty of Forestry, Universitas Sumatera Utara, Jl. Tri Dharma Ujung No. 1 Medan, North Sumatra, 20155, Indonesia.
Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan 20155, Indonesia.
Faculty of Pharmacy, Universitas Tjut Nyak Dhien, Medan, Indonesia.
Department of Forestry, Faculty of Forestry, Universitas Sumatera Utara, Jl. Tri Dharma Ujung No. 1 Medan, North Sumatra, 20155, Indonesia.
Department of Forestry, Faculty of Forestry, Universitas Sumatera Utara, Jl. Tri Dharma Ujung No. 1 Medan, North Sumatra, 20155, Indonesia.
Background: Secondary metabolites from the group of isoprenoid compounds are widely distributed in mangroveplants. Polyisoprenoids (dolichol and polyprenol) are known to have benefits as anticancer agents. The present studywas conducted to determine the cytotoxic potential of polyisoprenoids in leaves from seventeen selected mangrovespecies against colon cancer (WiDr) cells. Methods: Cytotoxic activity was evaluated by MTT assay in vitro usingWiDr human colon cancer cells and 3T3 fibroblasts from Swiss albino mouse embryo tissue as controls. Mechanismsof action were approached by assessing apoptosis and the cell cycle using flow cytometry and fluorescence microscopywith annexin V-FITC, as well as expression of Bcl-2 and cyclin D1 by immunocytochemistry. Results: Polyisoprenoidsfrom N. fruticans leaves demonstrated the highest anticancer activity, with an IC50 of 180.2 μg/mL, as compared to397.7 μg/mL against 3T3 normal cells. Significant decrease in the expression of Bcl-2 and cyclin D1 was also noted,facilitating apoptosis and arrest of the cell cycle in the G0-G1 phase in WiDr cells. The present study showed for thefirst time that polyisoprenoids from N. fruticans exhibit concrete anticancer activity in vitro, decreasing cell proliferationand inducing apoptosis in colon cancer cells. Conclusions: Polyisoprenoids isolated from N. fruticans leaves may havepromise as a source of anticancer agents.
https://journal.waocp.org/article_77405_9476ed6720f8a0a9f12ed3fcc8e9e1df.pdf
Antiproliferative
Apoptosis
Dolichol
Nypa fruticans
mangrove
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3401
3407
10.31557/APJCP.2018.19.12.3401
80099
Utilization of Colorectal Cancer Screening among Saudi Elderly Population: A Study from the Saudi National Survey for Elderly Health
Abdullah Khoja
atkhoja@imamu.edu.sa
1
Mohammad Aljawadi
maljawadi@ksu.edu.sa
2
Sulaiman A Al-Shammari
sulaiman@ksu.edu.sa
3
Nader N Bokhari
nabokhari@sfh.med.sa
4
Abdulrahman A Aldarwish
abadarwish@sm.imamu.edu.sa
5
Waleed K Mardini
wkmardini@sm.imamu.edu.sa
6
Tawfik A Khoja
khojatawfik@gmail.com
7
Department of Public Health and Family Medicine, College of Medicine, Al Imam Mohammad ibn Saud Islamic University (IMSIU), Riyadh, Kingdom of Saudi Arabia.
Department of Clinical Pharmacy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
Department of Family and Community Medicine, College of Medicine, King Saud University, Riyadh, Kingdom of Saudi Arabia.
Emergency Medicine, Security Forces Hospital, Health Ministers’ Council for the Gulf Cooperation Council States Riyadh, Saudi Arabia.
College of Medicine, Al Imam Mohammad ibn Saud Islamic University (IMSIU), Riyadh, Kingdom of Saudi Arabia.
College of Medicine, Al Imam Mohammad ibn Saud Islamic University (IMSIU), Riyadh, Kingdom of Saudi Arabia.
Former Director General, Executive Board, Health Ministers’ Council for the Gulf Cooperation Council States Riyadh, Saudi Arabia.
Objective: The goal of this study was to measure colorectal cancer screening (CRCS) utilization in Saudi Arabia ’selderly population and to assess the factors associated with CRCS. Methods: The Saudi National Survey for ElderlyHealth was used to examine CRCS utilization. It is a nationally representative population-based cross-sectional surveythat was conducted between 2006-2007. Utilization of CRCS was defined as any colonoscopy during the last five years orfecal occult blood test (FOBT) during the twelve months before the interview. Multivariable logistic regression was usedto assess patients’ demographics, co-morbidities, number of visits to primary health clinics, and hospital availabilityand accessibility impact on CRCS. Results: The prevalence of CRCS utilization among Saudi elderly population was5.64%. The fecal occult blood test was done in 4.4% of subjects while scope use was performed in 0.55%. In addition,0.69% of patients have gone through both FOBT and scope use. Having blood in stools (OR=2.80; 95%CI: 1.3-6.00),Self-drivers (OR= 2.52) private driver (OR=2.1; 95%CI: 1.15-3.7) having 4 or more visits to primary care centers 1.81(95%CI: 1.14-2.86) were positively associated with CRCS utilization. On the other hand, being single was negativelyassociated with CRCS utilization. Conclusion: In this nationally representative sample CRCS prevalence was verylow. According to our findings and in the context of the burden of colorectal cancer on the population, we recommenddeveloping national evidence-based policies and programs that take in consideration easiness of transportation and theavailability of primary care centers near to Saudi elderly population.
https://journal.waocp.org/article_80099_8e8d8735738463e373391fba6b20f63b.pdf
Saudi Arabia
Colon cancer
older adults
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3409
3413
10.31557/APJCP.2018.19.12.3409
80030
Relationship between Health Literacy and Adherence to Recommendations to Undergo Cancer Screening and Health-Related Behaviors among Insured Women in Japan
Eiko Goto
gotoue-tky@umin.ac.jp
1
Hirono Ishikawa
hirono-tky@umin.ac.jp
2
Tsuyoshi Okuhara
okuhara-ctr@umin.ac.jp
3
Takahiro Kiuchi
tak-kiuchi@umin.org
4
Department of Health Communication, School of Public Health, The University of Tokyo, Tokyo, Japan.
Department of Health Communication, School of Public Health, The University of Tokyo, Tokyo, Japan.
Department of Health Communication, School of Public Health, The University of Tokyo, Tokyo, Japan.
Department of Health Communication, School of Public Health, The University of Tokyo, Tokyo, Japan.
Background: Health literacy serves as a major barrier to effective preventive health behaviors, such as cancerscreening, and this relationship has not been studied among insured women in Japan. We examined the relationshipbetween health literacy and adherence to recommendations to undergo cancer screening, and health-related behaviors,among Japanese women who were insured by a health insurance society. Methods: We conducted a cross-sectionalobservation study of 670 insured Japanese women. For this, we used a self-administered questionnaire to assessenvironments, self-rated health status, cancer screening behavior, health-related behaviors (dietary behavior, exercisefrequency, alcohol consumption and smoking behavior), and health literacy. Results: Among the participants, 206completed the questionnaire (response rate, 30.7%). Fifty-seven had undergone breast and/or cervical cancer screening.The mean health literacy score was 3.44 (standard deviation = 0.68). In logistic regression models adjusted for age,self-rated economic status, and having a primary care physician, there was no statistically significant relationship betweenhealth literacy and adherence to recommendations to undergo cancer screening, and health-related behaviors. However,age and having a primary care physician were significantly associated with cancer screening and health-related behaviors.Health literacy was not found significantly associated with effective preventive health behaviors. Conclusions: Thepresent study found no statistically significant relationship between health literacy and adherence to recommendationsto undergo cancer screening and health-related behaviors. Rather, cancer screening and health-related behaviors werefound related to medical support from physicians and those their own age. Further study is needed for exploring theseassociations among insured women in Japan.
https://journal.waocp.org/article_80030_cf95427c31138cc9855a3390ad6844df.pdf
Health literacy
cancer screening
Health-related behavior
Japanese women
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3415
3425
10.31557/APJCP.2018.19.12.3415
80090
Association of Single Nucleotide Polymorphisms (SNPs) in Genes Encoding for Folate Metabolising Enzymes with Glioma and Meningioma in Indian Population
Rajani Kumawat
dr.rajanikumawat@gmail.com
1
Srinivas gowda
messagesrinih@gmail.com
2
Ekta Debnath
debnathekta@yahoo.in
3
Safoora Rashid
safoo.rashid@gmail.com
4
Ram Niwas
drrniwas@gmail.com
5
Yakhlesh Gupta
gyakhlesh@gmail.com
6
Ashish Datta Upadaya
aduaiims@rediffmail.com
7
Ashish Suri
surineuro@gmail.com
8
P Sarat Chandra
saratpchandra@gmail.com
9
Deepak K Gupta
drdeepakgupta@gmail.com
10
Ramakrishnan Lakshmy
lakshmy_ram@yahoo.com
11
Chitra Sarkar
sarkar.chitra@gmail.com
12
Subrata sinha
sub_sinha@hotmail.com
13
Kunzang Chosdol
kcaiimsbiochem@gmail.com
14
Department of Biochemistry, AIIMS, New Delhi, India.
Department of Biochemistry, AIIMS, New Delhi, India.
Department of Biochemistry, Lady Harding Medical College, New Delhi, India.
Department of Gastroenterology and Nutrition Medicine, AIIMS, New Delhi, India.
Department of Pulmonary Medicine, AIIMS, Jodhpur, India.
Department of Biochemistry, AIIMS, New Delhi, India.
Department of Biostatistics, AIIMS, India.
Department of Neurosurgery, AIIMS, New Delhi, India.
Department of Neurosurgery, AIIMS, New Delhi, India.
Department of Neurosurgery, AIIMS, New Delhi, India.
Department of Cardiac Biochemistry, AIIMS, New Delhi, India.
Department of Pathology, AIIMS, New Delhi, India.
Department of Biochemistry, AIIMS, New Delhi, India.
Department of Biochemistry, AIIMS, New Delhi, India.
Background: The association of primary brain tumors with Single Nucleotide polymorphisms (SNPs) in genes offolate metabolising enzymes have been reported to vary among different ethnic population. Here, we have studied theassociation of SNPs of folate metabolizing genes with the primary brain tumors (glioma and meningioma) in North Indianpopulation. Methods: SNPs of genes coding for folate metabolizing enzymes was carried out in 288 study populationfrom North India [Glioma (n=108), Meningioma (n=76) and healthy-control (n=104)]. The allele-specific polymerasechain reaction (ARMS-PCR) was used to analyse the SNP A1298C of the MTHFR (Methylenetetrahydrofolate-reductase)and the SNP A66G of the methionine synthase reductase (MTRR) genes. The PCR-RLFP (Restriction Fragment LengthPolymorphism) was used to analyse the SNP C677T of the Methylene tetrahydrofolate-reductase and the SNP A2756Gof the methionine-synthase (MTR) genes. Serum homocysteine, vitamin B12 and folate levels were evaluated in controls/patients serum using Chemiluminescence immunoassay and the levels were correlated with SNPs genotype. Results:The CC genotype of MTHFR A1298C was observed to have reduced risk of having meningioma than AA genotype(odd ratio=0.62, 95%CI 0.32-0.97, p=0.03). Similarly, the AG genotype of MTRR A66G showed reduced risk ofglioma than AA genotype (odd ratio=0.56, 95%CI 0.32-0.97, p=0.039). Furthermore, in patients with AA genotype ofMTR A2756G and CT genotype of MTHFR C677T showed higher serum homocysteine level than GG genotype (8.6μmol/L, p=0.048) and CC genotype (11.2μmol/L, p=0.039) respectively. Conclusion: Our findings provide an insightinto the risk association of SNPs in MTHFR A1298C and MTRR A66G genes with glioma/meningioma patients.Further studies are needed to evaluate their clinical implications.
https://journal.waocp.org/article_80090_55c9c9da313f1fd58cb87fd3aeaf1ce0.pdf
SNP
Glioma
Indian
homocysteine
Folate
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3427
3434
10.31557/APJCP.2018.19.12.3427
77406
Melittin Induced G1 Cell Cycle Arrest and Apoptosis in Chago-K1 Human Bronchogenic Carcinoma Cells and Inhibited the Differentiation of THP-1 Cells into Tumour- Associated Macrophages
Chartsiam Tipgomut
synchro.nize.130@gmail.com
1
Arin Wongprommoon
bht9216@gmail.com
2
Emi Takeo
emi_takeo@bio.eng.osaka-u.ac.jp
3
Teeranai Ittiudomrak
teeranai.itt@hotmail.com
4
Songchan Puthong
songchan.p@chula.ac.th
5
Chanpen Chanchao
chanpen.c@chula.ac.th
6
Department of Biology, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Bangkok, Thailand.
Homerton College, University of Cambridge, Hills Road, Cambridge CB2 8PH, UK.
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka, Japan.
Program in Biotechnology, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Bangkok, Thailand.
Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, 254, Phayathai Road, Bangkok, Thailand.
Department of Biology, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Bangkok, Thailand.
Background: Bronchogenic carcinoma (lung cancer) is one of the leading causes of death. Although manycompounds isolated from natural products have been used to treat it, drug resistance is a serious problem, and alternativeanti-cancer drugs are required. Here, melittin from Apis mellifera venom was used, and its effects on bronchogeniccarcinoma cell proliferation and tumour-associated macrophage differentiation were evaluated. Methods: The halfmaximal inhibitory concentration (IC50) of melittin was measured by MTT. Cell death was observed by annexin Vand propidium iodide (PI) co-staining followed by flow cytometry. Cell cycle arrest was revealed by PI staining andflow cytometry. To investigate the tumour microenvironment, differentiation of circulating monocytes (THP-1) intotumour-associated macrophages (TAMs) was assayed by sandwich-ELISA and interleukin (IL)-10 levels were determined.Cell proliferation and migration was observed by flat plate colony formation. Secretion of vascular endothelial growthfactor (VEGF) was detected by ELISA. The change in expression levels of CatS, Bcl-2, and MADD was measured byquantitative RT-PCR. Results: Melittin was significantly more cytotoxic (p < 0.01) to human bronchogenic carcinomacells (ChaGo-K1) than to the control human lung fibroblasts (Wi-38) cells. At 2.5 μM, melittin caused ChaGo-K1cells to undergo apoptosis and cell cycle arrest at the G1 phase. The IL-10 levels showed that melittin significantlyinhibited the differentiation of THP-1 cells into TAMs (p < 0.05) and reduced the number of colonies formed in thetreated ChaGo-K1 cells compared to the untreated cells. However, melittin did not affect angiogenesis in ChaGo-K1cells. Unlike MADD, Bcl-2 was up-regulated significantly (p < 0.05) in melittin-treated ChaGo-K1 cells. Conclusion:Melittin can be used as an alternative agent for lung cancer treatment because of its cytotoxicity against ChaGo-K1cells and the inhibition of differentiation of THP-1 cells into TAMs.
https://journal.waocp.org/article_77406_47ba9fcd1fa1734bb148764f50eb9529.pdf
Apoptosis
bronchogenic carcinoma
melittin
tumor-associated macrophage
cathepsin S
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3435
3441
10.31557/APJCP.2018.19.12.3435
77407
Platelet-to-Lymphocyte Ratio and Large Tumor Size Predict Microvascular Invasion after Resection for Hepatocellular Carcinoma
Narongsak Rungsakulkij
narongsak.run@mahidol.ac.th
1
Somkit Mingphruedh
somkit.m@gmail.com
2
Wikran Suragul
wikran.srg@gmail.com
3
Pongsatorn Tangtawee
pongsatornmd@gmail.com
4
Paramin Muangkaew
ra064@hotmail.com
5
Suraida Aeesoa
suraida.aee@mahidol.ac.th
6
Department of Surgery, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
Department of Surgery, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
Department of Surgery, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
Department of Surgery, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
Department of Surgery, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
Department of Surgery, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
Background: Recurrence after curative resection of hepatocellular carcinoma (HCC) is associated with early deathand poor prognosis. Microvascular invasion (mVI) is strongly associated with disease recurrence. Although many studieshave examined the relationship between various serum inflammatory indices and post-treatment prognosis, little isknown about preoperative predictors of microvascular invasion in HCC. Methods: Patients who underwent curativehepatic resection for HCC at our institute from January 2006 to December 2016 were retrospectively reviewed. Theassociations between mVI and various potential risk factors, including tumor size, hepatitis B and C virus infection,Child–Pugh scores, platelet-to-lymphocyte ratio, and neutrophil-to-lymphocyte ratio, were analyzed. Optimal cut-offvalues were determined using receiver operating characteristic curves. Results: A total of 330 HCC patients wereenrolled in this study, of whom 74 (22.4%) had tumors with mVI. After univariate analysis, two parameters weresignificantly associated with mVI after hepatic resection: platelet-to-lymphocyte ratio ≥102 (odds ratio [OR] 2.385,p = 0.001) and tumor size ≥5 cm (OR 4.29, p < 0.001). Both variables remained significant risk factors for mVI aftermultivariate analysis: platelet-to-lymphocyte ratio ≥102 (OR 1.831, p = 0.034) and tumor size ≥5 cm (OR 3.791,p < 0.001). Conclusions: Large tumor size (≥5 cm) and high platelet-to-lymphocyte ratio (≥102) are independentpredictive factors for mVI in HCC.
https://journal.waocp.org/article_77407_a1d216a87e1d2759d7f7178ec991af0a.pdf
Hepatocellular carcinoma
risk factors
platelet-to-lymphocyte
Prognosis
microvascular
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3443
3449
10.31557/APJCP.2018.19.12.3443
76645
Effect of Consultation on Adherence to Clinical Breast Examination and Mammography in Iranian Women: A Randomized Control Trial
Ameneh Mirmohammadirad
mirmohammadirad.a@gmail.com
1
Parisa Parsa
pparsa2003@yahoo.com
2
Batul Khodakarami
zahrabatul2006@yahoo.com
3
Ghodratalah Roshnaei
gh.roshanaei@umsha.ac.ir
4
Chronic Diseases (Home Care) Research Center, Department of Mother and Child Health, Hamadan University of Medical Sciences, Hamadan, Iran.
Chronic Diseases (Home Care) Research Center, Department of Mother and Child Health, Hamadan University of Medical Sciences, Hamadan, Iran.
Department of Midwifery, Hamadan University of Medical Sciences, Hamadan, Iran.
Modeling of Non-Communicable Diseases Research Center, Department of Biostatistics and Epidemiology, School of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran.
Background: Early diagnosis of breast cancer is directly related to success in treatment. The aim of this study wasto determine the effect of consultation based on the health belief model on performance of clinical breast examination(CBE) and mammography in women. Methods: This research was a clinical trial study. Eligible women aged> 40years attending to Hamadan health care centers in 2016 were randomly assigned to experimental and control groups(n = 75 in each group). The experimental group received 4 weekly sessions of breast cancer screening consulting basedon Health Belief Model (HBM). Knowledge on breast cancer, HBM constructs, and practices were compared betweentwo groups before, one and three months after intervention. Results: Before the intervention, no significant differenceswere observed in knowledge, HBM constructs and practice between experimental and control groups. While one andthree months post intervention significant differences were detected between two groups on HBM constructs (exceptsusceptibility and severity) and knowledge (p <0.05). Conclusions: The results showed the consultation promotedbreast cancer screening in women.
https://journal.waocp.org/article_76645_b218bebd1065cf5895d4725f796bccb7.pdf
breast cancer
Screening
consultation
Health Belief Model
Iran
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3451
3455
10.31557/APJCP.2018.19.12.3451
77409
Analysis of Polymorphism and Expression Profile of ASIC1 and IL-6 Genes in Patients with Gastric Cancer
Abdulkuddous Heydari-Mehrabadi
haydary1365@yahoo.com
1
Dor Mohammad Kordi-Tamandani
dor_kordi@science.usb.ac.ir
2
Tayebeh Baranzehi
kiana8588@gmail.com
3
Simin Hemati
esmatrigi@gmail.com
4
Department of Biology, University of Sistan and Baluchestan, Zahedan, Iran.
Department of Biology, University of Sistan and Baluchestan, Zahedan, Iran.
Department of Biology, University of Sistan and Baluchestan, Zahedan, Iran.
Department of Radiation Oncology, Isfahan University of Medical Science, Isfahan, Iran.
Abstract: Gastric cancer is one of the most common upper gastrointestinal malignancies. Some Iranian provinces,such as in the northern and northwestern areas, are at a high risk, whereas the central and western provinces are ata medium and the southern regions at low risk. This study was carried out to estimate the impact of the expressionpatterns of ASIC1 and IL-6 genes and the IL-6rs-174 and ASIC1rs 75624685 polymorphisms in the pathogenesis ofgastric cancer. Materials and methods: Tetra-ARMS PCR was employed to analyze the polymorphism status of theASIC1 and IL-6 genes with 85 paraffin-embedded tissue blocks from cases and 117 normal blood samples as controls.We also investigated mRNA expression levels of these genes in 12 cases and controls using real-time PCR. Results:Our results showed a significant association between expression of ASIC1 and elevated risk of gastric cancer (p<0.001).
https://journal.waocp.org/article_77409_d61c81d9b0116c7b432ba8b983b06032.pdf
Gastric cancer
Polymorphism
Expression
IL-6
ASIC1
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3457
3462
10.31557/APJCP.2018.19.12.3457
80076
Psychometric Properties of the Persian Version of the Sibling Cancer Needs Instrument (SCNI)
Zeynab Masoudifar
z.masoodi1392@gmail.com
1
Leila Khanali Mojen
leilakhanali@yahoo.com
2
Maryam Rassouli
rassouli.m@gmail.com
3
Maliheh Nasiri
malihenasiri@gmail.com
4
Pandora Patterson
pandora.patterson@canteen.org.au
5
Fiona Mc Donald
fiona.mcdonald@canteen.org.au
6
Peyman Eshghi
peshghi64@gmail.com
7
School of Nursing and Midwifery, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
School of Nursing and Midwifery, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
School of Nursing and Midwifery, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
School of Nursing and Midwifery, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Can Teen, The Australian Organisation for Young People Living with Cancer, Sydney, Australia.
Can Teen, The Australian Organisation for Young People Living with Cancer, Sydney, Australia.
Pediatric Congenital Hematologic Disorders Research Center, Research Institute for Children’s Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
The sibling cancer needs instrument (SCNI) is the first developed specifically for assessing psychosocial unmetneeds of adolescents having a sibling with cancer. The aim of this study is to evaluate the psychometric properties of itsPersian version. Methods: For this methodological study, the SCNI was translated into Persian using back-translationand revised according to the comments of the developer of the instrument. Then face validity, content validity, constructvalidity, internal consistency and the stability of the Persian version of the instrument were measure, by examininga population of 180 adolescents having a sibling with cancer in six hospitals in Tehran, Iran. The data were analyzedusing SPSS version 16 and EQS version 6.1. Results: Confirmatory factor analysis approved the construct validity ofthe instrument and its seven domains. Cronbach’s alpha was measured as 0.97 for the total instrument and 0.80- 0.92for its seven domains. In order to evaluate ttest-retest reliability, the intra-class correlation coefficient (ICC) was alsocalculated (0.94). Conclusions: The Persian version of SCNI has acceptable psychometric properties. It can be usedfor measuring the unmet psychosocial needs in adolescents who have a sibling with cancer in the Persian-speakingpopulation.
https://journal.waocp.org/article_80076_3eb9c09efabd360ee6291adaaa9fc292.pdf
Psychometrics
cancer
Adolescent
sibling
needs assessment
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3463
3466
10.31557/APJCP.2018.19.12.3463
80032
Expression of PAX-5 in B Cell Hodgkin and Non Hodgkin Lymphoma
Muhammad Rahil Khan
muhammad.rahil@hotmail.com
1
Arsalan Ahmad
2
Naila Kayani
3
Khurram Minhas
4
Department of Pathology, Bilawal Medical College, Liaquat University of Medical and Health Sciences, Pakistan.
Department of Pathology, Bilawal Medical College, Liaquat University of Medical and Health Sciences, Pakistan.
Department of Pathology, Bilawal Medical College, Liaquat University of Medical and Health Sciences, Pakistan.
Department of Pathology, Bilawal Medical College, Liaquat University of Medical and Health Sciences, Pakistan.
Background: B-cell malignancies including Precursor B-cell lymphoblastic lymphoma/leukemia and HodgkinLymphoma show a wide spectrum of B-cell differentiation from early stage B-cell precursors to mature B-cells ending interminal differentiation to plasma cells. Pan-B-cell antigens routinely used for the diagnosis of B-cell lymphoma, includeCD19, CD20, CD22 and CD79a.PAX-5 protein, also known as B-cell-specific activation protein is a B-cell-specifictranscription factor; essential for commitment and functional maintenance used in the diagnosis of B cell Hodgkinand non-Hodgkin lymphoma. PAX-5 show nuclear positivity in B cell lymphomas and moderate (dim) positivity inHodgkinlymphoma Reed Sternberg cells make this marker ideal for diagnosing B cell malignances. Objective: Todetermine the expression of PAX-5 in B cell Hodgkin and non-Hodgkin Lymphoma in order to improve the diagnosisof B-cell lymphomas. Methods: In this Prospective study, all the cases of B cell lymphoma diagnosed at The AgaKhan University Hospital, Karachi from July 2010 to July 2011were included. A panel of Immunohistochemical stainwas performed in all cases along with additional PAX- 5 stain with appropriate controls. Results: Total 125 cases wereincluded. Hodgkin Lymphoma (Mixed cellularity) was the commonest B-cell lymphoma subtype, 32 (25%) cases.Other common subtypes included Hodgkin lymphoma (Nodular sclerosis subtype), diffuse large B-cell lymphomaand B lymphoblastic lymphoma. Conclusion: This study demonstrates that PAX-5 is the most sensitive and reliableimmuhohistochemicalmarker in the diagnosis of B cell Hodgkin and non-Hodgkin lymphoma.
https://journal.waocp.org/article_80032_885801f5e5aa2368cb30ccc577a37dde.pdf
KEYWORDS: PAX-5
Hodgkin lymphoma
Non Hodgkin Lymphoma
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3467
3471
10.31557/APJCP.2018.19.12.3467
77410
Probably Benign Solid Breast Lesions on Ultrasound: Need for Biopsy Reassessed
Kulsoom Fatima
kulsoom.fatima@aku.edu
1
Imrana Masroor
imrana.masroor@aku.edu
2
Samina Khanani
samina.khanani@aku.edu
3
Department of Radiology, Aga Khan University, Karachi, Pakistan.
Department of Radiology, Aga Khan University, Karachi, Pakistan.
Department of Radiology, Aga Khan University, Karachi, Pakistan.
Objective: To determine the negative predictive value of ultrasound for breast masses with probably benignmorphology, and to assess whether follow-up may be an acceptable alternative to biopsy. Methods: After InstitutionalReview Board approval, all solid breast masses categorized as probably benign (American College of Radiology BreastImaging Reporting and Data System [BI-RADS] 3) on ultrasound from January 2014 to December 2015, and havingeither tissue diagnosis or imaging stability for 24 months, or downgrading to BIRADS 2 during imaging surveillancewere included. Result: A total of 157 lesions in 40 patients constituted the study population. The mean patient age was31.3 years (range, 20-56 years). Seventeen of these 157 lesions underwent tissue diagnosis with no invasive breast cancer.Out of the remaining 140 lesions, 115 were stable on imaging for 24 months or more. The rest 25 were deemed benignbecause of decrease in size on follow up (n=1), non-recommendation of further imaging by the second radiologist onfollow up ultrasound (n= 13) or presence of benign tissue diagnosis in the largest lesion (n=11). Conclusion: Ultrasoundhas 100% negative predictive value for breast lesions with probably benign morphology, whether palpable or not.Follow up is an appropriate option to immediate biopsy of such lesions keeping in mind that noncompliance withsurveillance may be a potential problem.
https://journal.waocp.org/article_77410_bf0e1505a770294ca29a31e067ba2092.pdf
Keywords: BI-RADS
probably benign
biopsy
Ultrasound
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3473
3477
10.31557/APJCP.2018.19.12.3473
77411
Molecular Targeting of Notch Signaling Pathway by DAPT in Human Ovarian Cancer: Possible Anti Metastatic Effects
Maryam Akbarzadeh
marya.akbarzadeh@gmail.com
1
Maryam Majidinia
majidinia.1359@gmail.com
2
Sedigheh Fekri Aval
fekrise@tbzmed.ac.ir
3
Soltanali Mahbub
soltanalimahbub@gmail.com
4
Nosratollah Zarghami
zarghami@tbzmed.ac.ir
5
Department of Biochemistry, Higher Education Institute of Rab-Rashid, Tabriz, Iran.
Solid Tumor Research Center, Urmia University of Medical Science, Urmia, Iran.
Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Biochemistry, Higher Education Institute of Rab-Rashid, Tabriz, Iran.
Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
Background: Ovarian cancer is one of the most important gynecological malignancies, causing significant mortality.Recently, there has been extensive attention to the involvement of signaling cascades in its initiation/progression. In thisstudy, we focused on the possible role of Notch signal transduction in proliferation and metalloproteinase 2 and 9 functionin human ovarian cancer OVCAR-3 cells. Methods: MTT proliferation assays were used to evaluate effects of a DAPTinhibitor on cell proliferation. For measurement of Hes-1 mRNA levels, quantitative reverse transcription polymerasechain reaction (qRT-PCR) was applied following 48 h incubation with the inhibitor. In addition, metalloproteinase(MMPs) activity was assessed by zymography. Results: Inhibition of Notch signaling resulted in a significant reductionin OVCAR-3 cell proliferation. Additionally, DAPT treatment of cells significantly decreased Hes-1 mRNA levels(p < 0.05) as well as activity of MMP-2 and -9 (p < 0.05). Conclusion: Our results suggested that suppression of Notchsignaling by a specific inhibitor can effectively decrease proliferation and the potential for metastasis of OVCAR-3 cellsvia a reduction in the activity of metalloproteinases 2 and 9. Thus, pharmacological targeting of the Notch signalingpathway could be a promising future treatment for ovarian cancer.
https://journal.waocp.org/article_77411_084e1b8c027dd7fa5f7a3ed82a6ace46.pdf
Notch signaling pathway
Metalloproteinases
Ovarian Cancer
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3479
3488
10.31557/APJCP.2018.19.12.3479
77049
Induction of Apoptosis by an Extract of Persian Gulf Marine Mollusc, Turbo Coronatus through the Production of Reactive Oxygen Species in Mouse Melanoma Cells
Fatemeh Zangeneh
fazanganeh@gmail.com
1
Amir Vazirizadeh
vaziriamir@yahoo.com
2
Mohammad Reza Mirshamsi
mohammad.mirshamsi@gmail.com
3
Amir Fakhri
amirfakhri@yahoo.com
4
Mehrdad Faizi
faizi64@yahoo.com
5
Jalal Pourahmad
j.pourahmadjaktaji@utoronto.ca
6
Department of Pharmacology and Toxicology, Faculty of Pharmacy and Pharmaceutical Sciences, Research Center Shahid Beheshti University of Medical Sciences, Tehran, Iran.
The Marine Biology and Fishery Science Department, Persian Gulf Institute, Persian Gulf University, Bushehr, Iran.
Department of Pharmacology and Toxicology, Faculty of Pharmacy and Pharmaceutical Sciences, Research Center Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Pharmacology and Toxicology, Faculty of Pharmacy and Pharmaceutical Sciences, Research Center Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Pharmacology and Toxicology, Faculty of Pharmacy and Pharmaceutical Sciences, Research Center Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Pharmacology and Toxicology, Faculty of Pharmacy and Pharmaceutical Sciences, Research Center Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Objective: A variety of approaches such as surgery, chemotherapy, radiotherapy, hormonal therapy andimmunotherapy are used to treat melanomas, but unfortunately in most case, the response is very weak and often sideeffects are serious. This study concerns selective toxicity of an extract of Turbo coronatus on cells and mitochondria froma syngeneic mouse model of melanoma. Methods: Cells and mitochondria isolated from extra tumoral and melanomatissues were exposed toa T. coronatus crude extract and fractions obtained by gel-filtration chromatography and assayedfor mitochondrial and cellular parameters. Result: Crude extract (375, 750 and 1,500 μg/ml) and fraction 1; F1; (275,550 and 1100 μg/ml) of T. coronatus extract induced a significant (p<0.05) increase of the reactive oxygen species(ROS) level, swelling of mitochondria, collapse of mitochondrial membrane potential (MMP), release of cytochromec and caspase-3 activation only in the mitochondria and cells obtained from melanoma but not extra tumoral tissues. Inaddition, the F1 fraction decreased the percentage of viable cells and induced apoptosis in melanoma cells. Conclusion:For the first time we could demonstrate that the F1 fraction of a T. coronatus extract, selectively induces ROS mediatedcytotoxicity by directly targeting mitochondria in melanoma tissues and it may be a suitable candidate for novel drugtreatment of malignant melanomas.
https://journal.waocp.org/article_77049_9856713c5344fb70bb1e28d813edf823.pdf
Melanoma
Turbo coronatus
mitochondria
Apoptosis
Gel filtration
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3489
3493
10.31557/APJCP.2018.19.12.3489
79153
5-Year Survival Rates and Prognostic Factors in Patients with Synchronus and Metachronus Breast Cancer from 2010 to 2015
Arash Sarveazad
arashsarveazad@gmail.com
1
Asrin Babahajian
babahajian3@yahoo.com
2
Jebreil shamsadin
shams.jebreil@gmail.com
3
Mansour Bahardoust
mansourbahari93@gmail.com
4
Colorectal Research Center, Iran University of Medical Sciences, Tehran, Iran.
Liver and Digestive Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Infectious and Tropical Diseases Research Center, Hormozgan Health Institute, Hormozgan University of Medical science, Bandar Abbas, Iran.
Colorectal Research Center, Iran University of Medical Sciences, Tehran, Iran.
Introduction: Currently breast (BC) cancer is a serious medical problem in all countries of the world. Survivaldepends on many factors. The present study focused on 5-year survival and its related factors in patients with BC inIran. Material and methods. The present analytical retrospective study was performed (from March 2010 until March2015) on patients with BC followed for at least 6 months. The main variables assessed were tumor size, grade of lymphnode involvement, metastasis, stage, history, human epidermal growth factor receptor expression, and tumor origin.Analysis of survival was accomplished using the Kaplan- Meier method. Results: Some 351 (80.2%) of the total of438 individuals had unilateral and 87(19.8%) had bilateral cancer, 28 (35.6%) of the latter being synchronous and56(64.4%) metachronous. Mean duration of follow-up was 47.44±28.19 months, during which 61 (17.3%) patientswith unilateral and 18 with bilateral cancer eventually died. The 5-year survival rate in patients with unilateral BC wassignificantly higher than those with bilateral BC (Log-rank Test chi2= 3.11, p=0.032). In addition, with metachronouscases, the survival rate was 64.2% in comparison with 51.6% for synchronous BCs. Survival rate was significantly(p value =0.038) higher with metachronous than with synchronous cancers (Log-rank Test chi2=3.54, p=0.038). Thehighest survival rate was reported for BCs originating from lobule tissue and the lowest rate examples of interstitialtissue origin (Log-rank Test chi2=11.54, p=0.0001). Patients with earl stage lesions (M1) survived longer than withother stages (Log-rank Test chi2= 9.55, p=0.001). Conclusion: In this study, most women with BC had a positivefamily history and were married. The 5-year survival rate was lower with advanced stages of cancer. According to ourfindings, survival rates might improve if patients undergo screening and diagnosis is made at an early stage of the disease.
https://journal.waocp.org/article_79153_8d01a770aed3be4954e47f90a1ad3be8.pdf
breast cancer
survival
Metastasis
clinical stage
Iran
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3495
3500
10.31557/APJCP.2018.19.12.3495
80042
Outcomes and Complications of Simultaneous Laparoscopic Cystectomy and Laparoscopic Nephroureterectomy with Umbilical Reduced Port Surgery
Yutaro Tanaka
yuyu_tanaka@hotmail.com
1
Takehiko Okamura
hiko2546@kosei.anjo.aichi.jp
2
Ryosuke Chaya
brutus.you.are.fool.r.c@gmail.com
3
Takashi Nagai
tkshng73@gmail.com
4
Daichi Kobayashi
daichikoba1979@yahoo.co.jp
5
Takahiro Kobayashi
kobayachinko@yahoo.co.jp
6
Hidetoshi Akita
fqpfb961@gmail.com
7
Takahiro Yasui
yasui@med.nagoya-cu.ac.jp
8
Department of Urology, Anjo Kosei Hospital, 28 Higashihirokute, Anjo-cho, Anjo, Japan.
Department of Urology, Anjo Kosei Hospital, 28 Higashihirokute, Anjo-cho, Anjo, Japan.
Department of Urology, Anjo Kosei Hospital, 28 Higashihirokute, Anjo-cho, Anjo, Japan.
Department of Urology, Anjo Kosei Hospital, 28 Higashihirokute, Anjo-cho, Anjo, Japan.
Department of Urology, Anjo Kosei Hospital, 28 Higashihirokute, Anjo-cho, Anjo, Japan.
Department of Urology, Anjo Kosei Hospital, 28 Higashihirokute, Anjo-cho, Anjo, Japan.
Department of Urology, Anjo Kosei Hospital, 28 Higashihirokute, Anjo-cho, Anjo, Japan.
Department of Nephro-Urology, Nagoya City University Graduate School of Medical Sciences, Kawasumi 1, Mizuho-cho, Mizuho-ku, Nagoya, Japan.
Objective: In recent years, although reduced port surgeries (RPS) have been reported for many urological diseases,there have been no reports regarding simultaneous laparoscopic cystectomy and unilateral or bilateral nephroureterectomywith umbilical RPS. Therefore, the aim of this study was to evaluate outcomes and complications of simultaneouslaparoscopic cystectomy and unilateral or bilateral nephroureterectomy with umbilical RPS. Methods: We performeda preliminary case series of 4 patients with synchronous upper urinary tract (UUT) tumor and invasive bladder cancerwho underwent simultaneous laparoscopic cystectomy and unilateral or bilateral nephroureterectomy with umbilical RPSbetween 2014 and 2017 at our hospital. Demographic data, pathologic features, the surgical technique, and outcomeswere retrospectively analyzed. Result: All 4 patients were men whose median age was 79 years (range 65-85 years) andmedian body mass index was 24.2 kg/m2 (range 21.5-27.3 kg/m2). The laparoscopic approach was technically successfulin all 4 patients without the need for open conversion. The median total operative time was 434 minutes (range 372-481minutes). The median estimated blood loss was 773 ml (range 153-923 ml), median interval to resuming oral intakewas 2 days (range 1-7 days), and median hospital stay was 16 days (range 13-20 days). Conclusion: The reduced portapproach is technically feasible in terms of many outcome measures, with significant cosmetic advantages. This methodcan be performed safely and recommended as a viable option for patients with concomitant UUT and bladder cancer.
https://journal.waocp.org/article_80042_7395d99f6b4be489d3524ec757aa0b28.pdf
Cystectomy
nephroureterectomy
Umbilical approach
reduced port surgery
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3501
3509
10.31557/APJCP.2018.19.12.3501
80077
Anticancer Activity of Camel Milk via Induction of Autophagic Death in Human Colorectal and Breast Cancer Cells
Roopesh Krishnankutty
rkrishnankutty@hamad.qa
1
Ahmad Iskandarani
aiskandarani@hamad.qa
2
Lubna Therachiyil
ltherachiyil@hamad.qa
3
Shahab Uddin
skhan34@hamad.qa
4
Fouad Azizi
fazizi@hamad.qa
5
Michael Kulinski
mkulinski@hamad.qa
6
Ajaz Ahmad Bhat
ajaz.bhatt@gmail.com
7
Ramzi M Mohammad
mohammad@karmanos.org
8
Translational Research Institute, Academic Health System, Hamad Medical Corporation, Doha, State of Qatar.
Translational Research Institute, Academic Health System, Hamad Medical Corporation, Doha, State of Qatar.
Translational Research Institute, Academic Health System, Hamad Medical Corporation, Doha, State of Qatar.
Translational Research Institute, Academic Health System, Hamad Medical Corporation, Doha, State of Qatar.
Translational Research Institute, Academic Health System, Hamad Medical Corporation, Doha, State of Qatar.
Translational Research Institute, Academic Health System, Hamad Medical Corporation, Doha, State of Qatar.
Translational Research Institute, Academic Health System, Hamad Medical Corporation, Doha, State of Qatar.
Translational Research Institute, Academic Health System, Hamad Medical Corporation, Doha, State of Qatar.
Background/ Objective: Camel milk is traditionally known for its human health benefits and believed to be a remedyfor various human ailments including cancer. The study was aimed to evaluate the inhibitory effects of commerciallyavailable camel milk on cancer cells and its underlying mechanism(s). Materials and Methods: Two cell lines:colorectal cancer HCT 116 and breast cancer MCF-7 were cultured with different doses of camel milk. The effects ofcamel milk on cell death were determined by MTT assay, viability by trypan blue exclusion assay and migration by invitro scratch assay. The mechanism was elucidated by western blotting and confocal microscopy was used to confirmautophagy. Results: Camel milk significantly reduced proliferation, viability as well as migration of both the cells.The accumulation of LC3-II protein along with reduction in expression of p62 and Atg 5-12, the autophagy proteinsimplied induction of autophagy. The (GFP)-LC3 puncta detected by confocal microscopy confirmed the autophagosomeformation in response to camel milk treatment. Conclusion: Camel milk exerted antiproliferative effects on humancolorectal HCT 116 and breast MCF-7 cancer cells by inducing autophagy.
https://journal.waocp.org/article_80077_7e3e1b16ebe93b4c57f3778071e5a2d6.pdf
colorectal cancer
breast cancer
Camel Milk
Autophagy
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3511
3515
10.31557/APJCP.2018.19.12.3511
76652
Enzymatic antioxidant and lipid peroxidation evaluation in the newly diagnosed breast cancer patients in Iran
Parisa Kangari
1
Tahereh Zarnoosheh Farahany
2
Ali Golchin
agolchin.vet10@yahoo.com
3
Somayeh Ebadollahzadeh
4
Arash Salmaninejad
5
Soltan Ali Mahboob
6
Alireza Nourazarian
7
Department of Biology, higher education institute of Rab – Rashid, Tabriz, Iran.
Department of Biology, School of Advanced Technologies in Medicine, Islamic Azad University Medical Branch of Tehran, Tehran, Iran.
Department of Tissue Engineering and Applied Cell Sciences, Student Research Committee, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Tissue engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Medical Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Tissue engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of biochemistry and clinical Laboratories, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Background: Breast cancer is caused by breast tissue malignant cells and it has become one of the main medical concerns with a socio-economic significance especially for women. Among the multiple factors involved in the initiation, progression, and invasion of breast cancer, oxidative stress plays an important role. Antioxidant status, lipid peroxidation, and oxidative stress in newly diagnosed breast cancer patients were determined to find a defined pattern of oxidative stress in these patients. Methods: The malondialdehyde (MDA) levels (as an indicator of lipid peroxidation), glutathione peroxidase (GPX), and superoxide dismutase (SOD) activities of newly diagnosed breast cancer patients (n=38) and controls (n=38) were assessed using blood samples. Results: MDA level and SOD activity were significantly higher in the breast cancer patients compared to the healthy subjects group (p
https://journal.waocp.org/article_76652_3b36f65cb54ee471edf87cb63754c0f7.pdf
breast cancer
Lipid peroxidation
oxidative stress
Superoxide dismutase
Glutathione peroxidase
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3517
3523
10.31557/APJCP.2018.19.12.3517
80141
An Automatic Bone Disorder Classification Using Hybrid Texture Feature Extraction with Bone Mineral Density
Ramkumar S
sram0829@gmail.com
1
Malathi R
2
Department of EIE, Annamalai University, India.
Department of EIE, Annamalai University, India.
A novel approach has been proposed to classify bone disorders for classifying the radiographic bone image asnormal, Osteopenia and Osteoporosis. The proposed system consists of three major stages to predict the accurate bonedisorder classification. In the first stage, image preprocessing is performed where bilateral filtering is applied to removenoise and to enhance the image quality. Then, the image is fed to Otsu based segmentation approach for segmentingthe abnormal area of the bone image. In the second stage, Discrete Wavelet Transform (DWT) is used to the segmentedimage. Once the image gets segmented then, the Gray-Level Co-occurrence Matrix (GLCM) method is applied to extractthe features in terms of statistical texture-based. Further the image which is applied to Principle Component Analysis(PCA) to reduce size of the feature vector. Besides, Bone Mineral Density (BMD) feature namely calcium volume isestimated from abnormal region in the segmented bone image and it is concatenated with the extracted texture featuresto obtain the final feature vectors. In the final stage, the Multi-class Support Vector Machine (MSVM) takes featurevectors as a inputto classify bone disorders. The simulation result demonstrates that the proposed system achieved theaccuracy of 95.1% and sensitivity of 96.15%.
https://journal.waocp.org/article_80141_185c6fdd4f26ad59dfce22d96e29736a.pdf
Osteoporosis
osteopenia
DWT (Discrete Wavelet Transform)
GLCM (Gray-Level Co-occurrence Matrix)
MSVM (Multi-class Support Vector Machine)
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3525
3531
10.31557/APJCP.2018.19.12.3525
80108
The Role of Diuretics in Treatment of Aromatase Inhibitors Induced Musculoskeletal Symptoms in Women with Non Metastatic Breast Cancer
Alshimaa Mahmoud Alhanafy
alshimaa_alhanafy@yahoo.com
1
Alaa Labeeb
2
Ashraf Khalil
3
Department of Clinical Oncology and Nuclear Medicine, Faculty of Medicine, Menoufia University, Shebin El kom, Egypt.
Department of Rheumatology, Physical Medicine and Rehabilitation, Faculty of Medicine - Menoufia University, Shebin El Kom, Egypt.
Department of Clinical Chemistry, National Liver Institute, Menoufia University, Shebin El kom, Egypt.
Background: Around 50% of women receiving Aromatase Inhibitors (AIs) develop musculoskeletal symptomswhich may become severe causing interruption of treatment. Patients with AI-induced arthralgia had higher rates ofjoint effusions and fluid in the tendons, so use of diuretics may be helpful. Methods: This prospective phase II studywas conducted in department of clinical oncology and nuclear medicine, Menoufia University Hospital, Egypt, betweenJan. to Dec. 2015. Fifty Women with stage I,II and III breast cancer receiving AIs as adjuvant hormonal treatmentcomplaining of AIs related musculoskeletal symptoms received Lasilactone® 50 mg tablet; (an oral combination ofFrusemide 20mg/Spironolactone 50 mg), every other day for 4 weeks. Patients were assessed by modified WesternOntario and McMaster Universities osteoarthritis (WOMAC) index for lower limb and the quick Disabilities of theArm, Shoulder and Hand Score (DASH) scoring system for upper limbs, Arabic versions, at baseline and after 4 weeksof treatment. Results: The mean WOMAC pain score improved significantly (6.0 v 10; P < 0.001), the mean WOMACstiffness score improved (2.3 v 3.9; P = 0.002), the mean WOMAC functional score improved (8.7 v 15; P < 0.001),the total WOMAC score improved (17 v 29; P < 0.001), also a significant difference was noticed for the quick DASHscore; total score (16 v 25; P = 0.02) After use of diuretics for 4 weeks of treatment compared with baseline scores.Conclusions: The use of diuretics effectively reduces AI related musculoskeletal symptoms with good tolerance
https://journal.waocp.org/article_80108_0775eba40490716d95e2cc187834c372.pdf
Diuretics
Aromatase inhibitors
Musculoskeletal Symptoms
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3533
3543
10.31557/APJCP.2018.19.12.3533
77413
A Pharmacological Strategy Using Stemofoline for more Efficacious Chemotherapeutic Treatments Against Human Multidrug Resistant Leukemic Cells
Sonthaya Umsumarng
s.umsumarng@gmail.com
1
Sariya Mapoung
srmapoung@gmail.com
2
Supachai Yodkeeree
yodkeelee@hotmail.com
3
Stephen G Pyne
spyne@uow.edu.au
4
Pornngarm Limtrakul Dejkriengkraikul
pornngarm.d@cmu.ac.th
5
Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
School of Chemistry, University of Wollongong, Wollongong, New South Wales, Australia.
Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Our previous study reported that stemofoline (STF) exhibited a synergistic effect with chemotherapeutic drugs inhuman multidrug-resistant (MDR) leukemic cells (K526/Adr) by inhibiting the function of P-glycoprotein, which is amembrane transporter that is overexpressed in several types of MDR cancers. This study further investigated the effects ofa combination treatment of STF and doxorubicin (DOX) in vitro and in vivo. The combination treatment of 50 mg/kg ofSTF strongly enhanced the anti-tumor activity of DOX in SCID-beige mice bearing K562/Adr xenografts withoutadditional toxicity when compared to the single treatment groups. Additionally, an examination of the proliferationmarkers (Ki67) and the apoptotic marker (TUNEL) in tumor tissues in each group revealed that the combinationtherapy significantly reduced Ki67 positive cells and increased apoptotic cells. From the in vitro experiments we alsofound that this combination treatment dramatically induced G1 and G2M arrest in K562/Adr when compared to a singletreatment of DOX. STF treatment alone did not show any cytotoxic effect to the cells. These results suggest that theaccumulation of DOX enhanced by STF was sufficient to induce cell cycle arrest in K562/Adr. These findings supportour previous in vitro data and indicate the possibility of developing STF as an adjuvant therapy in cancer treatments.
https://journal.waocp.org/article_77413_1516b7cea93e1b5d03105b56943066f3.pdf
Chemotherapy
Leukemia
p-glycoprotein
Xenograft model
Stemofoline
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3545
3550
10.31557/APJCP.2018.19.12.3545
80053
Rate and Predictive Factors for Sustained Complete Response after Selective Transarterial Chemoembolization (TACE) in Patients with Hepatocellular Carcinoma
Kittipitch Bannangkoon
drkittipitch@gmail.com
1
Keerati Hongsakul
hkeerati@medicine.psu.ac.th
2
Teeravut Tubtawee
ttubtawee@gmail.com
3
Edward Mc Neil
edward.m@psu.ac.th
4
Hutcha Sriplung
hutcha.s@gmail.com
5
Virasakdi Chongsuwiwatvong
cvirasak@gmail.com
6
Department of Radiology, Faculty of Medicine, Prince of Songkla University, 15 Kanchanavanit Road, Hat Yai, Songkhla, Thailand.
Department of Radiology, Faculty of Medicine, Prince of Songkla University, 15 Kanchanavanit Road, Hat Yai, Songkhla, Thailand.
Department of Radiology, Faculty of Medicine, Prince of Songkla University, 15 Kanchanavanit Road, Hat Yai, Songkhla, Thailand.
Epidemiology Unit, Faculty of Medicine, Prince of Songkla University, 15 Kanchanavanit Road, Hat Yai, Songkhla, Thailand.
Epidemiology Unit, Faculty of Medicine, Prince of Songkla University, 15 Kanchanavanit Road, Hat Yai, Songkhla, Thailand.
Epidemiology Unit, Faculty of Medicine, Prince of Songkla University, 15 Kanchanavanit Road, Hat Yai, Songkhla, Thailand.
Background: To determine the effectiveness and performance of selective conventional transarterialchemoembolization (TACE) and analyze the potential predictive factors of sustained complete response (CR) forpatients with hepatocellular carcinoma (HCC). Materials and Methods: Total of 52 patients with HCC (33 males,19 females; mean age 64.0 ± 9.6 years) who underwent 81 sessions of selective TACE between November 2015 andMarch 2017 at Songklanagarind hospital were reviewed. The Kaplan-Meier method was used to describe CR rates atvarious time points. Univariate and multivariate logistic regression models were performed to determine the predictivefactors for sustained CR at six months. Results: The CR rates after selective TACE at 1, 4, 6, 9 and 12 months were87%, 81%, 62%, 40% and 31%, respectively. Univariate and multivariate analyses demonstrated that alpha fetoproteinlevel <100ng/ml, a tumor size in summation ≤ 30 mm, ≤ 2 sessions of selective TACE and unilobar involvement had asignificantly higher odds of sustaining complete response at six months (p =0.018, 0.031, 0.032, and 0.044, respectively).Conclusions: Selective TACE has a good therapeutic results and can sustained complete response in selected HCCpatients. Serum AFP≤ 100 ng/ml, a few sessions of selective TACE, tumor size in summation ≤ 30 mm and unilobarinvolvement were favorable predictive factors for sustained complete response of HCC patients.
https://journal.waocp.org/article_80053_d9ddc98f53c39fc9a71cd3fb3f059da8.pdf
complete response
predictive factors
selective TACE
Hepatocellular carcinoma
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3551
3555
10.31557/APJCP.2018.19.12.3551
80050
Malignancy in Systemic Lupus Erythematosus (SLE) Patients
Rizna Abdul Cader
rizna_c@hotmail.com
1
Amy Khoo Mei Yee
amykhoo0101@gmail.com
2
Azrul Yassin
azrulreesha@gmail.com
3
Ismail Ahmad
m_dsparekusa2004@yahoo.com
4
Siti Nurfatin Haron
fateen_krusty@yahoo.com
5
Department of Medicine, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, Bandar Tun Razak, Cheras, Kuala Lumpur, Malaysia.
Department of Medicine, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, Bandar Tun Razak, Cheras, Kuala Lumpur, Malaysia.
Department of Medicine, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, Bandar Tun Razak, Cheras, Kuala Lumpur, Malaysia.
Department of Medicine, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, Bandar Tun Razak, Cheras, Kuala Lumpur, Malaysia.
Department of Medicine, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, Bandar Tun Razak, Cheras, Kuala Lumpur, Malaysia.
Background: Malignancies are among the leading causes of death in Systemic Lupus Erythematosus (SLE)patients with studies reporting a higher prevalence of malignancy in SLE patients compared to the general population.We wanted to determine the frequency of cancer in a cohort of SLE patients and identify its associated risk factors.Methods: Cross-sectional study involving SLE patients attending the nephrology outpatient clinic, UniversitiKebangsaan Malaysia Medical Centre between January and June 2014. Results: We recruited 228 patients (207 female,21 male), aged 40.48 ± 12.86 years with mean SLE duration of 11.65 ± 6.46 years. Majority (87%) had lupus nephritisand were in remission with a median SLEDAI score 2 (0, 14). Majority (89%) were on corticosteroid with either asteroid sparing agent like mycophenolate mofetil (15.4%), azathioprine (36.8%) or ciclosporin (15.4%). One hundredand sixty (70.2%) patients were either receiving or had received intravenous cyclophosphamide with median doseof 5,173.6 ± 3,242.4 mg. Seven female patients were diagnosed with cancer during the course of their SLE with 56(34-78) years being median age at malignancy and SLE duration of 4 (0-12) years. Majority (5/7) had lupus nephritisand all patients a median dose of prednisolone 10 (2.5, 10) mg with 10 (4-24) years of steroids. Two patients had afamily history of cancer with majority developing cancer after the diagnosis of SLE. Two patients received intravenouscyclophosphamide prior to the development of cancer for their SLE compared to overall cohort of 160. Three patientshad colorectal cancer, 2 had cervical cancer, 1 had breast cancer, and one patient had germ cell tumour and one thyroidcancer. All patients had their cancer successful treated with no signs of recurrence. Conclusion: We found a loweroccurrence of cancer in our SLE patients as compared with the reported literature.
https://journal.waocp.org/article_80050_ec99670a2f0f0f20bb04025ed019be8a.pdf
cancer
Cyclophosphamide
lupus nephritis
Malignancy
Systemic lupus erythematosus
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3557
3564
10.31557/APJCP.2018.19.12.3557
77418
Prognostic Value of BCL2 in Women Patients with Invasive Breast Cancer
E-Z Ayadi
ayadiemna90@gmail.com
1
B Cherif
boutheina.cherif.cbs@gmail.com
2
Y Ben Hamed
youssef.benhamed@yahoo.fr
3
N Ben Yahia
nejib.benyahia@gmail.com
4
M Mokni
moncefmokni@gmail.com
5
A Rebai
ahmed.rebai@gmail.com
6
H Ayadi
ayadi.hammadi@gmail.com
7
R Jlidi
rachid.jlidi@gmail.com
8
Procédés de criblage moléculaire et cellulaire, Centre of Biotechnology of Sfax B.P K.3038 Sfax, Tunisia.
Procédés de criblage moléculaire et cellulaire, Centre of Biotechnology of Sfax B.P K.3038 Sfax, Tunisia.
Patholab Private Cytopathology Laboratory Dar elhkim route touristique 4190 homtessouk, Djerba,Tunisia.
Laboratoire d’anatomie et de cytologie pathologique, EPS Farhat Hached Hospital, Sousse,Tunisia.
Patholab Private Cytopathology Laboratory A. Ibn Khaldoun Sfax, Tunisia.
Procédés de criblage moléculaire et cellulaire, Centre of Biotechnology of Sfax B.P K.3038 Sfax, Tunisia.
Procédés de criblage moléculaire et cellulaire, Centre of Biotechnology of Sfax B.P K.3038 Sfax, Tunisia.
Patholab Private CytoPathology Laboratory R. du Caire, Cité Jardin Sfax,Tunisia.
Background: Breast cancers are heterogeneous, making it essential to recognize several biomarkers for canceroutcome predictions especially in young women where the classical prediction parameters are not suitable. The goalfrom this study is to evaluate the impact of B cell lymphoma 2 (BCL2), P53 and Ki-67 proteins expression on survivalin young women patients with invasive ductal carcinoma. Patients and methods: Samples and clinical data from 238patients were collected between 2003 and 2017. They were selected according to 2 criteria: age ≤40 years old and most ofthem are affected by an Invasive Ductal Carcinoma. We evaluated BCL2, P53 and ki-67 expression by immunochemistrytest, and then we assessed correlations of these biomarkers expression with patient’s clinicopathological characteristicsand survival. Results: Triple negative breast cancer group showed a high frequency among our cohort but we emphasizean almost equitable distribution among all molecular groups. Contrary to other studies which reported that luminal Awas correlated with better prognosis, our analysis demonstrated that luminal A is correlated with the Scarff, Bloomand Richardson (SBR) grading 2 or SBR grading 3. To better investigate the prognosis, we analyze three biomarkersknown by their impact on physiopathology behavior on breast cancer BCL2, ki-67and P53. BCL2 is the more relevantone, it was correlated with molecular subtypes (p=0.0012) and SBR grading (p=0.0016). BCL2 seems to be the goodprognostic biomarker related to survival (p=0.004) with a protective role among patients when endocrine therapyis not provided and Lymph Node (LN) involvement is positive (p=0.021, p=0.000 respectively). Conclusions: Theclassical prognostic parameters based mainly on the molecular classification in breast cancer seem insufficient in thecase of young women. BCL2 protein expression analysis provides a better prognostic value. BCL2 should be clinicallyassociated in current practice when young women specimens are diagnosticated.
https://journal.waocp.org/article_77418_5f2774f69a886ad44570240df86d0ae7.pdf
breast cancer
BCL2
Young Women
Prognosis
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3565
3569
10.31557/APJCP.2018.19.12.3565
80143
Helicobacter pylori Infection and Related Gastrointestinal Diseases in Southeast Asian Countries: An Expert Opinion Survey
Duc Trong Quach
drquachtd@gmail.com
1
Ratha-korn Vilaichone
vilaichone@hotmail.co.th
2
Khien Van Vu
khienvuvan@hotmail.com
3
Yoshio Yamaoka
yyamaoka@bcm.edu
4
Kentaro Sugano
sugano@jichi.ac.jp
5
Varocha Mahachai
varocha.m@gmail.com
6
Department of Internal Medicine, University of Medicine and Pharmacy at Hochiminh City, Hochiminh, Vietnam.
Division of Gastroenterology, Department of Medicine, Thammasat University Hospital, Pathumthani, Thailand.
Department of Gastrointestinal Endosccopy, 108 Central Military Hospital, Hanoi, Vietnam.
Department of Medicine, Gastroenterology Section, Baylor College of Medicine and Michael E. DeBakey VA Medical Center, Texas, USA.
Department of Internal Medicine, Division of Gastroenterology, Jichi Medical University, Japan.
National Gastric Cancer and Gastrointestinal diseases Research Center, Pathumthani Thailand.
Background: Helicobacter pylori (H. pylori) infection is currently considered as an infectious disease irrespective ofsymptoms and stage of disease. This study aimed to survey the impact of H. pylori infection and the current managementapproaches in Southeast Asian countries. Materials and methods: This is a survey among 26 experts from 9 SoutheastAsian countries (Cambodia, Indonesia, Laos, Malaysia, Myanmar, Philippines, Singapore, Thailand and Vietnam),who attended a meeting to develop the ASEAN consensus on H. pylori management in November 2015. Results: Theprevalence of H. pylori varied significantly from 20% to 69% among countries, highest in Myanmar and lowest inMalaysia. The rate of H. pylori infection in patients with gastritis, peptic ulcer disease and gastric cancer (GC) alsovaried significantly, not only among countries but also among regions within the same country. The most commonmethod for H. pylori diagnosis before treatment was rapid urease test, followed by urea breath test. In multi-ethniccountries, some ethnic groups including Chinese, Batak and Minahasanese were considered as having higher risk ofGC. There have been no national screening programs for GC in all countries, and a majority of patients with GC werediagnosed in advanced stages with very poor 5-year survival. Conclusions: The prevalence of H. pylori infection andits infection rates in related gastrointestinal diseases were significantly different among Southeast Asian countries. Theprognosis of patients with GC in the region was very poor. The result of this survey is a platform for future internationaland regional research collaboration.
https://journal.waocp.org/article_80143_9595c87bc0bd96f8afb13e60703b2e0d.pdf
Helicobacter pylori
Gastritis
peptic ulcer disease
Gastric cancer
Southeast Asia
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3571
3580
10.31557/APJCP.2018.19.12.3571
77414
Automatic Approach for Cervical Cancer Detection and Segmentation Using Neural Network Classifier
Elayaraja P
sunandmun@gmail.com
1
Suganthi M
msuganthib@gmail.com
2
Department of Ece, Kongunadu College of Engineering and Technology, Trichy, Tamilnadu, India.
Head of Ece, Mahendra College of Engineering, Salem, India.
Cervical cancer leads to major death disease in women around the world every year. This cancer can be cured if itis initially screened and giving timely treatment to the patients. This paper proposes a novel methodology for screeningthe cervical cancer using cervigram images. Oriented Local Histogram Technique (OLHT) is applied on the cervicalimage to enhance the edges and then Dual Tree Complex Wavelet Transform (DT-CWT) is applied on it to obtainmulti resolution image. Then, features as wavelet, Grey Level Co-occurrence Matrix (GLCM), moment invariantand Local Binary Pattern (LBP) features are extracted from this transformed multi resolution cervical image. Theseextracted features are trained and also tested by feed forward back propagation neural network to classify the givencervical image into normal and abnormal. The morphological operations are applied on the abnormal cervical imageto detect and segment the cancer region. The performance of the proposed cervical cancer detection system is analyzedin the terms of sensitivity, specificity, accuracy, positive predictive value, negative predictive value, Likelihood Ratiopositive, Likelihood ratio negative, precision, false positive rate and false negative rate. The performance measures forthe cervical cancer detection system achieves 97.42% of sensitivity, 99.36% of specificity, 98.29% of accuracy, PPVof 97.28%, NPV of 92.17%, LRP of 141.71, LRN of 0.0936, 97.38 % precision, 96.72% FPR and 91.36% NPR. Fromthe simulation results, the proposed methodology outperforms the conventional methodologies for cervical cancerdetection and segmentation process.
https://journal.waocp.org/article_77414_8a2a6c34820acf3c43c34be2995b2052.pdf
Cervical cancer
Cervigram
Features
Gabor transforms
Neural Networks
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3581
3589
10.31557/APJCP.2018.19.12.3581
77419
Topoisomerase II α Gene alteration in Triple Negative Breast Cancer and Its Predictive Role for Anthracycline-Based Chemotherapy (Egyptian NCI Patients)
Mahitab Ibrahim Eltohamy
mahitabaltohami@yahoo.com
1
Omnia Mohammed Badawy
omniabadawy75@hotmail.com
2
Naglaa El kinaai
naglaa_elkinaai@yahoo.com
3
Iman Loay
imanloay@gmail.com
4
Hanan Ramadan Nassar
nhanan_67@yahoo.com
5
Rasha Mahmoud Allam
allam.rasha@yahoo.com
6
Mona Ali Sakr
m.sakr14263@gmail.com
7
Department of Pathology, National Cancer Institute, Cairo University, Egypt.
Department of Pathology, National Cancer Institute, Cairo University, Egypt.
Department of Pathology, National Cancer Institute, Cairo University, and Children’s Cancer Hospital Egypt, Cairo, Egypt.
Department of Pathology, National Cancer Institute, Cairo University, Egypt.
Department of Medical Oncology, National Cancer Institute, Cairo University, Egypt.
Department of Biostatistics and Cancer Epidemiology, National Cancer Institute, Cairo University, Egypt.
Department of Pathology, National Cancer Institute, Cairo University, Egypt.
Objective: Triple negative breast cancer is an aggressive variant of breast cancer; it forms about 15% of breastcancer cases. It lacks the responsiveness to hormonal and targeted therapies. Anthracyclines remain the treatment optionfor these patients. Anthracyclines are cardiotoxic, so predicting sensitivity of response by biological predictors mayhave a role in selecting suitable candidates for these drugs. Material and methods: This study included 50 TNBCcases, from National Cancer Institute, Cairo University(NCI-CU), Egypt, who underwent surgery and received adjuvantchemotherapy. Archived blocks were obtained and immunostaining for Ki-67 LI and Fluorescent In situ Hybridization(FISH) technique to assess TOP2A gene copy number and chromosome 17CEP status were done. Analysis of associationbetween TOP2A alterations and CEP17 polysomy as well as Ki-67 LI with other clinicopathological parameters wasdone. Associations between the biological markers and event free survival (EFS) and overall survival (OS), were alsoperformed. Results: TOP2A alteration was seen in 9/50 cases (5 amplified and 4 deleted). CEP17 Polysomy was detectedin 14% of cases. Most of patients (80%) showed Ki-67 LI ≥20%. There was a significant association between TOP2Agene and CEP17 status. Outcome was better with abnormal TOP2A gene status and CEP17 polysomy, radiotherapyand combined anthracyclines and taxanes in the adjuvant setting, however P-values were not significant. Conclusion:TOP2A gene alterations and CEP17 polysomy may have prognostic and predictive role in TNBC treated with adjuvantAnthracyclines.
https://journal.waocp.org/article_77419_437dcb24dc74faf79a05a38746a4f955.pdf
TOP2A
CEP17 polysomy
anthracyclines
TNBC
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3591
3596
10.31557/APJCP.2018.19.12.3591
77420
Features of Microcalcifications on Screening Mammography in Young Women
Atsushi Fushimi
fushimi@jikei.ac.jp
1
Naoko Fukushima
naoko9037@gmail.com
2
Toshimasa Suzuki
suzutoshi42@yahoo.co.jp
3
Rei Kudo
reisan42@gmail.com
4
Hiroshi Takeyama
takeyama@jikei.ac.jp
5
Department of Surgery, Sakuragaoka Hospital, Shizuoka, Japan.
Department of Surgery, Sakuragaoka Hospital, Shizuoka, Japan.
Department of Surgery, Sakuragaoka Hospital, Shizuoka, Japan.
Department of Surgery, The Jikei University School of Medicine, Tokyo, Japan.
Department of Surgery, The Jikei University School of Medicine, Tokyo, Japan.
Background: There is no decrease in the number of breast cancer deaths if screening mammography is performedin women aged hereditary breast cancer. Therefore, more accurate screening mammography for young women is needed. Objective: Toevaluate the features of screening mammographic findings, particularly microcalcifications, in women aged to increase the positive predictive value of screening mammography in young women. Methods: We retrospectivelyreviewed the data of consecutive women who underwent opportunistic and organized breast cancer screening at theSakuragaoka Hospital (Shizuoka, Japan) between April 2013 and March 2015. We compared the mammographicfindings and features of microcalcifications between women aged Results: The study included 3645 women. Of these 3645 women, 415 (11.4%) were aged were aged 40–49 years, and 2011 (55.2%) were aged 50–74 years. Women aged recalled for microcalcifications than those aged 50–74 years (Young women were more likely to be recalled for small round and segmental microcalcifications [(OR): 1.799 (95% CI: 0.751–2.846); 40–49 years, OR: 1.394 (95% CI: 0.714–2.074)] and less likely to be recalled forsmall round and grouped microcalcifications [(95% CI: 0.496–1.428)] compared with women aged 50–74 years. Conclusions: On screening mammography, womenaged microcalcifications. False-positive results may be reduced by reflecting the characteristics of microcalcification findingsamong young women without breast cancer in the future.
https://journal.waocp.org/article_77420_b6f349a2b86d9a9787563704a4c6e963.pdf
mammography
breast cancer
Young Women
microcalcifications
Screening
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3597
3603
10.31557/APJCP.2018.19.12.3597
77050
Factors Affecting Survival in Neuroendocrine Tumors: A 15-Year Single Center Experience
Abdullah Sakin
drsakin@hotmail.com
1
Makbule Tambas
makbule_tambas@hotmail.com
2
Saban Secmeler
drsabansecmeler@hotmail.com
3
Orcun Can
orcuncan2004@yahoo.com
4
Serdar Arici
serdararici@hotmail.com
5
Nurgul Yasar
yasarnurgul@yahoo.com
6
Caglayan Geredeli
caglayange@hotmail.com
7
Cumhur Demir
cdemir@dr.com
8
Sener Cihan
sener70@yahoo.com
9
Department of Medical Oncology, Yuzuncu Yil University Medical School, 65090, Van, Turkey.
Department of Radiation Oncology, University of Health Sciences, Okmeydani Training and Research Hospital, 34384, Istanbul, Turkey.
Department of Medical Oncology, University of Health Sciences, Okmeydani Training and Research Hospital, 34384, Istanbul, Turkey.
Department of Medical Oncology, University of Health Sciences, Okmeydani Training and Research Hospital, 34384, Istanbul, Turkey.
Department of Medical Oncology, University of Health Sciences, Okmeydani Training and Research Hospital, 34384, Istanbul, Turkey.
Department of Medical Oncology, University of Health Sciences, Okmeydani Training and Research Hospital, 34384, Istanbul, Turkey.
Department of Medical Oncology, University of Health Sciences, Okmeydani Training and Research Hospital, 34384, Istanbul, Turkey.
Department of Medical Oncology, University of Health Sciences, Okmeydani Training and Research Hospital, 34384, Istanbul, Turkey.
Department of Medical Oncology, University of Health Sciences, Okmeydani Training and Research Hospital, 34384, Istanbul, Turkey.
Background: Neuroendocrine tumors are a heterogeneous group of tumors that can originate from all of theneuroendocrine cells in the body, mostly from the gastrointestinal tract. In addition to early diagnosis, streamingpatients into appropriate prognostic groups is an important component of treatment. In this study, we examined thefactors that affect survival in patients we followed in our center between 2000-2016. Methods: The demographic data,clinical and pathological features of patients were obtained from their medical files. TNM staging and tumor gradingwere performed according to AJCC and WHO 2010 classification. SPSS 15.0 for Windows programme was used forstatistical analysis. Results: 85 patients (32 male, 53 female) were included into the study. The median age of the patientswas 55,7 (27-83) years. Eighty percent of the tumors were of gastroenteropancreatic system, most commonly stomach(27.1%) origin. Nineteen patients (22.4%) died during follow-up. In univariate analysis; age (p<0,001), stage (p=0.002),primary tumor localization (p=0.005), grade (p<0.001), Ki-67 value (p<0.001), number of metastases (p=0.001) andtype of surgery (p<0.001) were found to be factors affecting survival. Age (p=0.024) and Ki67 (p <0.001) were theindependent prognostic factors for survival in multivariate analysis. For the cut-off value of 6%, Ki-67 had a sensitivityof 83.3% and specifity of 71.4% for survival determination. Conclusion: Ki-67 ratio and age were the most importantfactors affecting survival in neuroendocrine tumors in our study. Ki-67 ratio has a high sensitivity and specificity forpredicting survival, a cut-off value of 6% may be used to predict survival.
https://journal.waocp.org/article_77050_9287fce9b0419545df74b53707df05e0.pdf
age
Grade
Ki-67
Neuroendocrine tumor
survival
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3605
3613
10.31557/APJCP.2018.19.12.3605
80051
Rhinacanthin-C Extracted from Rhinacanthus nasutus (L.) Inhibits Cholangiocarcinoma Cell Migration and Invasion by Decreasing MMP-2, uPA, FAK and MAPK Pathways
Parichart Boueroy
joomjim_mc@hotmail.com
1
Sunitta Saensa‑Ard
nunit_94@hotmail.com
2
Pongpun Siripong
pongpun8@yahoo.com
3
Sakawrat Kanthawong
sakawrat@kku.ac.th
4
Chariya Hahnvajanawong
hchari@kku.ac.th
5
Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Natural Products Research Section, Research Division, National Cancer Institute; Bangkok, Thailand.
Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Cholangiocarcinoma is a malignant tumor with high metastatic and mortality rates. We investigated the effectsof rhinacanthin-C on cell proliferation, migration, invasion and the expression of proteins regulating cancer cellinvasion-regulated proteins in a cholangiocarcinoma (KKU-M156) cell line. Cytotoxicity of rhinacanthin-C wasdetermined by the SRB assay. Using wound-migration, chamber-migration and chamber-invasion assays, we assessedthe effects of rhinacanthin-C against KKU-M156 cells. The activities of matrix metalloproteinases 2 and 9 (MMP-2,MMP-9) and urokinase-type plasminogen activator (uPA) were determined using gelatinase and uPA zymographyassays. The expression of invasion-regulated proteins was investigated using western-blot analysis. After treatmentwith rhinacanthin-C, KKU-M156 cells exhibited antiproliferative effects in a dose-dependent manner with greaterefficacy than in Vero cells: IC50 values were 1.50 and 2.37 μM, respectively. Rhinacanthin-C significantly inhibited cellmigration and invasion of KKU-M156 cells in a dose-dependent manner. Consistent with this observation, treatmentwith rhinacanthin-C was associated with a decrease in the expression levels of FAK, p-FAK, MMP-2, and a decrease inthe levels of p38-, JNK1/2- and ERK1/2-MAPK pathways as well as inhibiting NF-κB/p65 expression and translocationof NF-κB/p65 to the nucleus. We have shown for the first time that the anti-metastatic effects of rhinacanthin-C onKKU-M156 cells are mediated via inhibition of the expression of invasion-regulated proteins. Rhinacanthin-C maydeserve consideration as a potential agent for the treatment of cholangiocarcinoma.
https://journal.waocp.org/article_80051_d02e21d71a9e0cc71c3d2102d9828579.pdf
Rhinacanthin-C
Invasion
migration
Metastasis
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3615
3621
10.31557/APJCP.2018.19.12.3615
80054
Diagnostic Accuracy of Axillary Ultrasonography Compared with Intra-operative Pathological Findings in Patients with Breast Cancer
Alireza Rezvani
rezvaniar@sums.ac.ir
1
Aryan Zahergivar
aryan_zaher123@yahoo.com
2
Pooya Iranpour
iranpour@sums.ac.ir
3
Majid Akrami
akramimd@yahoo.com
4
Sepehr Kazemi
sepehr_kazemi1992@yahoo.com
5
Breast Cancer Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Imaging Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Breast Cancer Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Breast Cancer Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Objective: Breast cancer is the most common cancer and leading cause of mortality in women worldwide. Diagnosis ofaxillary lymph node involvement, frequently by axillary ultrasonography (AUS), is an important step in patients withprimary breast cancer, while the gold standard pathological examination is only applicable during surgery. Thus,we aimed to evaluate the predictive value of AUS in detection of lymph node involvement. Methods: A total of 56patients with primary breast cancer, who were candidate of mastectomy were included in this study. A single radiologistperformed pre-operative axillary sonography for all the patients and reported the results in a checklist. The resultswere then compared with intra-operative pathological results of lymph node dissection to evaluate the sensitivity,specificity, positive and negative predictive values of AUS in detection of the disease. Result: The results showed thatpre-operative AUS had a sensitivity of 63.3%, specificity of 84.6%, positive predictive value of 82.6%, and negativepredictive value of 66.6%. Correlation between axillary lymph node characteristics revealed that the absence of fattyhilum, cortical thickness, and loss of ovality of the lymph nodes were the most specific factors in detection of lymphnodes’ involvement. Conclusion: AUS has a low sensitivity and cannot determine the presence of micro-metastasesand suspicious lymph nodes accurately in early stages of the disease, and it seems that defining new parameters mayhelp for increasing the sensitivity and specificity of the axillary lymph node sonography. Apparently, pathologicalexamination remains the gold standard diagnostic method.
https://journal.waocp.org/article_80054_779f1a6bec1192aa354d1c4ff0fa93e9.pdf
Breast Neoplasms
Ultrasonography
lymph nodes
Axilla
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3623
3627
10.31557/APJCP.2018.19.12.3623
77051
Lymph Node Ratio: Is It an Independent Prognostic Factor for Stage III Cutaneous Melanoma?
Abolfazl Salari
abolfazl_salari_70@yahoo.com
1
Fatemeh Nili
f-nili@sina.tums.ac.ir
2
Amir Mohsen Jalaeefar
jalaeefar@gmail.com
3
Mohammad Shirkhoda
mshirkhoda@sina.tums.ac.ir
4
Cancer Research Center, Cancer Institute, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran.
Department of Pathology, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran.
Department Of Surgery, Cancer Institute, Imam Khomeini Hospital Complex, Tehran University Of Medical Sciences, Tehran, Iran.
Cancer Research Center, Cancer Institute, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran.
Objective: Lymph node ratio (LNR) is defined as the ratio of the number of metastatic lymph nodes to the dissectedlymph nodes. LNR is a prognostic factor for many tumor types. The present study aimed to evaluate the prognosticvalue of LNR in melanoma. Methods: This retrospective cohort study was conducted on 123 patients with stage IIIcutaneous melanoma. Multivariate Cox proportional hazards model was used to evaluate the correlations between LNRand other clinicopathological factors associated with survival. The patients were divided into four groups in terms ofthe LNR, including groups A (LNR≤0.18), B (0.18<LNR≤0.37), C (0.37<LNR≤0.625), and D (LNR>0.625). Results:Initially, LNR was evaluated as a continuous quantity associated with survival. In the univariate analysis, a significantcorrelation was observed between LNR, overall survival (OS), and disease free survival (DFS). Meanwhile, the onlyassociation observed in the multivariate analysis was between LNR and OS. Increased LNR from group A to group Dreduced OS from 46 (±44.09) to 22.5 (±16.33) months (P=0.022). According to the multivariate analysis, prognosticfactors in OS were tumor thickness, American joint committee of cancer (AJCC) N stage, interferon administration,and undergoing chemotherapy. Conclusion: According to the results, LNR could be used as an independent prognosticfactor for estimating the survival of patients with stage III cutaneous melanoma also designing an effective adjuvanttreatment protocol for these patients.
https://journal.waocp.org/article_77051_f1d89d53333d830e77930152ebb71521.pdf
cutaneous melanoma
lymph node ratio
lymph node dissection
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3629
3634
10.31557/APJCP.2018.19.12.3629
77415
Risk Factors and Prognosis of Spontaneously Ruptured Hepatocellular Carcinoma in Thailand
Jitrapa Kerdsuknirun
jittu_tujit@yahoo.com
1
Virunpat Vilaichone
virunpat.vil@gmail.com
2
Ratha-Korn Vilaichone
vilaichone@hotmail.co.th
3
Gastroenterology Unit, Department of Medicine, Faculty of Medicine, Thammasat University Hospital, Pathumthani, Thailand.
Patumwan Demonstration School, Srinakharinwirot University, Bangkok,Thailand.
Gastroenterology Unit, Department of Medicine, Faculty of Medicine, Thammasat University Hospital, Pathumthani, Thailand.
Objective: Hepatocellular carcinoma (HCC) is the fatal cancer worldwide. Spontaneously Rupture remainsimportant complication of HCC. The incidence of spontaneous HCC rupture was high in ASEAN but limited studieswere reported. The aim of this study was to evaluate predictors and overall survival of ruptured HCC in tertiary carecenter in Thailand. Methods: We conducted a retrospective cohort study of HCC patients aged ≥15 years old duringJanuary 2012-January 2016 and followed up through June 2016 at Thammasat University Hospital, Thailand. Allclinical information, laboratory and radiologic findings were collected from reviewing computer data base of medicalrecords. Results: 333 patients had completely retrievable information. Of which, 51patients (15.3%) had spontaneousruptured HCC. Clinical symptoms with abdominal discomfort and anemic symptoms were significantly higher inruptured than non-ruptured HCC groups. (76.47% vs.39.36%: p<0.001, 13.73vs.0.71%, p<0.001). Furthermore, sizeof tumors, presences of metastasis and serum AFP>200 ng/mL were significantly higher in ruptured than non-rupturedHCC groups (10.29cm. vs. 6.47cm. p<0.001, 17.65% vs. 8.16%, p=0.034, 60.78% vs. 36.88%, p=0.001, respectively).In multivariate analysis, age, abdominal discomfort, anemic symptoms, Child-Pugh score>6 were independent riskfactors of HCC rupture (OR=0.96, 95%CI=0.93-0.99, p=0.02; OR=3.47, 95%CI=1.26-9.6, p=0.016; OR=54.51,95%CI=7.09-418.89, p<0.001; OR=2.62, 95%CI=1.09-6.31, p=0.031, respectively). 1-year and 2-year survival ratesof ruptured HCC were 66.9% and 44.6% respectively. Conclusions: Age, abdominal discomfort, anemic symptomsand Child-Pugh score>6 might be predictive factors of spontaneous ruptured HCC. Ruptures of HCC remained a fataldisease with poor survival rate in Thailand. Appropriate treatment in early stage could be effective tool to improve thetreatment outcomes
https://journal.waocp.org/article_77415_ee57f2e6297bb6c362cc09c7c3a6145a.pdf
Hepatocellular carcinoma
ruptured
Thailand
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-12-01
19
12
3635
3641
10.31557/APJCP.2018.19.12.3635
80075
Prevention of Malignant Transformation of Oral Leukoplakia and Oral Lichen Planus Using Laser: An Observational Study
Karandeep Singh Arora
drkaranarora@yahoo.com
1
Rahul Bansal
rahul85bansal@gmail.com
2
Shreeyam Mohapatra
shreeyam080@gmail.com
3
Akshay Verma
dr.akashyavermaop@gmail.com
4
Sakshi Sharma
sakshisharma639@gmail.com
5
Shubhangi Pareek
shubhi.pareek@gmail.com
6
Department of Oral Medicine and Radiology, M. M. College of Dental Sciences and Research, Mullana, Ambala, Haryana, India.
Department of Oral Medicine and Radiology, M. M. College of Dental Sciences and Research, Mullana, Ambala, Haryana, India.
2Department of Oral Medicine and Radiology, SCB Dental College and Hospital, Cuttack, Odisha, India.
Department of Oral Pathology and Microbiology, Rajasthan Dental College and Hospital, Jaipur, Rajasthan, India.
Department of Public Health Dentistry, Bhojia Dental College and Hospital, Bhud, Baddi, Himachal Pradesh, India.
Department of Oral Pathology and Microbiology, Institute of Dental Sciences, Bhubneshwar, Odisha, India.
Objectives: The present observational study was conducted with an aim to evaluate the efficacy of diode laser inmanagement of homogenous oral leukoplakia (OL) and reticular oral lichen planus (OLP), so that these potentiallymalignant disorders can be limited and further malignant transformation can be prevented. Further the assessmentof associated postoperative complications after laser therapy was also carried out. Study Design: Present study wascarried out using diode laser 810nm on 60 subjects, of whom 30 subjects were of homogenous OL and 30 subjectswere of reticular OLP aged between 20 – 60 years, the diagnosis of which was histopathologically confirmed. Results:Of the 60 subjects none complained of pain during and immediately after surgery with no bleeding at any stage of theprocedure. By the end of 3rd day post operative most subjects reported no pain and swelling and very few subjects hadnegligible pain and swelling when evaluated. On subsequent follow-up of 1,2 and 4 week(s) none of the subject reportedof pain, swelling or bleeding and it was noted that all the areas treated with laser had healed without scarring with nosigns of recurrence. Fibrosis was seen in two male patients and one female patient treated for OL and OLP respectively.Conclusion: Patient compliance and contentment without any postoperative complications were observed to be of highdegree in the present study. Thus diode laser can be considered as a best alternative to conventional surgical treatmentmodality in managing OL and OLP and preventing its further transformation.
https://journal.waocp.org/article_80075_f2f7abd7cb326420e8e4a9c4b33f5172.pdf
Diode laser
Oral leukoplakia
Oral lichen planus
potentially malignant lesions
Prevention