eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3189
3191
10.31557/APJCP.2019.20.11.3189
88846
Re: Repair Index in Examination of Nuclear Changes in the Buccal Mucosa of Smokers: A Useful Method for Screening of Oral Cancer
Armen Nersesyan
anersesyan@yahoo.com
1
Institute of Cancer Research, Medical University of Vienna, Austria.
https://journal.waocp.org/article_88846_9a037272679dcea815009a2b9d33cc08.pdf
smoking
buccal cells
micronucleus
nuclear anomalies
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3193
3196
10.31557/APJCP.2019.20.11.3193
88822
Helicobacter Pylori Detected in Tap Water of Peruvian Patients with Gastric Cancer
Miluska Castillo
miluskacastillogarcia@gmail.com
1
Luis Bernabe
luangelbm@gmail.com
2
Carlos A Castaneda
carloscastanedaaltamirano@yahoo.com
3
Ivan Chavez
ichavez@inen.sld.pe
4
Eloy Ruiz
ruizeloy@gmail.com
5
Fernando Barreda
fernando.barreda73@hotmail.com
6
Daniel Valdivia
dvaldivia@inen.sld.pe
7
Nancy Suarez
nan.suz55@gmail.com
8
Jais Nieves
jaisnievesp@gmail.com
9
Emmanuel Dias-Neto
emmanuel@accamargo.org.br
10
Kevin Boehnke
kboehnke@umich.edu
11
Maria P Landa-Baella
mariapilibaella@gmail.com
12
Paola Montenegro
pmontenegro@inen.sld.pe
13
Instituto Nacional de Enfermedades Neoplasicas, Lima, Peru.
Instituto Nacional de Enfermedades Neoplasicas, Lima, Peru.
Instituto Nacional de Enfermedades Neoplasicas, Lima, Peru.
Instituto Nacional de Enfermedades Neoplasicas, Lima, Peru.
Instituto Nacional de Enfermedades Neoplasicas, Lima, Peru.
Instituto Nacional de Enfermedades Neoplasicas, Lima, Peru.
Instituto Nacional de Enfermedades Neoplasicas, Lima, Peru.
Instituto Nacional de Enfermedades Neoplasicas, Lima, Peru.
Instituto Nacional de Enfermedades Neoplasicas, Lima, Peru.
CIPE, A.C.Camargo Cancer Center, São Paulo, Brazil.
University of Michigan, Ann Arbor, Michigan, USA.
Instituto Nacional de Enfermedades Neoplasicas, Lima, Peru.
Instituto Nacional de Enfermedades Neoplasicas, Lima, Peru.
Objective: To evaluate the correlation between the presence of H. pylori in paired samples of tap water and gastric cancer (GC) lesion in Lima city (Peru). Material and methods: Gastric tissue and tap-water samples were prospectively collected from 82 Gastric Cancer who lived in Lima. HspA and ureA genes were evaluated by qPCR in the samples. Results: The median age of patients with GC was 63 years, 52.4% were men and stage-II in 36.6%. A home-living time> 10 years was reported in 84.1% of patients. Boiling water treatment was indicated in 85.4% of cases. H. pylori was detected in 69.5% of gastric tissues and in 12.2% of analyzed tap-water. There was no differences in gastric infection rates among those with or without water contamination (70% vs. 69.4%, p=0.971). Conclusion & Impact: H. pylori was found in tap-water samples, however, detection rates were lower than in gastric cancer samples. Other sources of infection transmission should be investigated.
https://journal.waocp.org/article_88822_41061c1d5fa8327ee67a03508e209ead.pdf
Helicobacter pylori
cancer gastric
tap-water
ureA
hspA
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3197
3209
10.31557/APJCP.2019.20.11.3197
88832
Benefits of Aerobic Exercise for Breast Cancer Survivors: A Systematic Review of Randomized Controlled Trials
Amira Hassan Bekhet
1300096@st.pt.cu.edu.eg
1
Ahmed Ramadan Abdallah
ahmedramadan3687@gmail.com
2
Horeya M Ismail
horeya.ismail@gmail.com
3
Doaa M Genena
doaagenena@gmail.com
4
Nermin A Osman
nerminahmed@alexu.edu.eg
5
Ayman El Khatib
a.elkhatib@bau.edu.lb
6
Rami Labib Abbas
r.abbas@bau.edu.lb
7
Faculty of Physical Therapy, Cairo University, Cairo, Egypt.
Faculty of Medicine, Mansoura University, Mansoura, Egypt.
Medical Research Group of Egypt, Cairo, Egypt.
Medical Research Institute, Alexandria University, Alexandria, Egypt.
Medical Research Institute, Alexandria University, Alexandria, Egypt.
Department of Physical therapy, Faculty of Health Sciences, Beirut Arab University, Beirut, Lebanon.
Department of Physical therapy, Faculty of Health Sciences, Beirut Arab University, Beirut, Lebanon.
Background: Physical exercise may be beneficial to breast cancer (BC) survivors. Here, we systematically summarized the effects of aerobic exercise in BC survivors. We conducted a systematic review of randomized controlled trials (RCTs). Methods: We searched PubMed, Web of knowledge, Scopus, Cochrane Central, Virtual Health Library and PEDRO databases for relevant RCTs, comparing aerobic exercise with usual care among BC survivors. Data were extracted and evidence was synthesized narratively. Results: Twelve studies were included in this systematic review. Studies reported that aerobic exercise can significantly improve the quality of life in BC survivors. Moreover, aerobic exercise alleviated the symptoms of depression and anxiety. However, current evidence from the included studies showed that there was no significant benefit for aerobic exercise in terms of weight loss. Conclusion: Our study suggests that aerobic exercise is beneficial to BC survivors. Clinical Relevance: Aerobic exercise should be recommended in the therapeutic and rehabilitative regimens of BC survivors.
https://journal.waocp.org/article_88832_fbac4a649da2dd21b4fab2774da8e93c.pdf
Aerobic exercise
breast cancer
physical activity
Quality of Life
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3211
3219
10.31557/APJCP.2019.20.11.3211
88748
Effect of Occupational Exposure to Radar Radiation on Cancer Risk: A Systematic Review and Meta-Analysis
Ali Safari Variani
safary2400@yahoo.com
1
Somayeh Saboori
saburi_somaye@yahoo.com
2
Saeed Shahsavari
biostat93@gmail.com
3
Saeed Yari
smaeedy@gmail.com
4
Vida Zaroushani
v.zaroushani@qums.ac.ir
5
Department of Occupational Health Engineering, Faculty of Health, Qazvin University of Medical Sciences, Qazvin, Iran.
Department of nutrition, Lorestan University of Medical Sciences, Lorestan, Iran.
Instructor of Biostatistics, Health Product Safety Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.
Student Research Committee, Department of Faculty of Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Occupational Health Engineering, Faculty of Health, Qazvin University of Medical Sciences, Qazvin, Iran.
Objective: Microwave radiation is one of the most growing environmental workplace factors that exposes too many workers in the various workplaces. Regard to concerns about cancer incidence in these workers and lack of systematic or meta-analytic studies about this object, so, we conducted a meta-analysis to acquire an understanding of the association between cancer risk and occupational exposure to radar radiation. Methods: A systematic search was carried out on case-control, cohort and clinical control trial studies that published in the Cochrane Library, PubMed, ISI Web of Science, Scopus and Google scholar databases that accomplished from March 2017 to March 2018 and updated on 30 September, 2018 in English and Persian articles without time limit in publication date. Keywords were selected based on PICO principle and collected from MeSH database. After removal of duplicated studied, taking into inclusion and exclusion criteria, the process of screening was carried out and data were extracted after preparation of the full text of included articles. Article collection was completed by manually searching for a reference list of eligible studies. For quality assessment of included studies, Newcastle-Ottawa scale was used. Results: a total of 533 studies was found in the first step of literature search, only 6 were included with 53,008 sample size according to inclusion and exclusion criteria. Estimated pooled random effects size analysis showed no significant increasing effect of occupational exposure to radar radiation on mortality rate (MR=0.81, 95%CI: 0.78, 0.83) and relative risk (RR=0.87, 95%CI: 0.75, 0.99, P <0.0001) of cancer with a significant heterogeneity between the selected studies. Conclusions: In conclusion, the results of this meta-analysis study have shown no significant increase in overall mortality ratio and cancer risk ratio from occupational exposure to the radar frequency of workers. But, these results are not conclusive. As regards to some limitation such as fewer numbers of included studies, lack of data about exposure characterizations and demographic characterizations in this meta-analysis, this result is not certain and conclusive. It is recommended to conduct future studies.
https://journal.waocp.org/article_88748_b00ea38e804eabfd6a8445d9f4c71973.pdf
Occupational cancer
OCCUPATIONAL EXPOSURE
Radar
neoplasm
Meta-analysis
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3221
3231
10.31557/APJCP.2019.20.11.3221
88817
Cancer Incidence and Mortality in Firefighters: A State-of-the-Art Review and Meta-َAnalysis
Elpidoforos S Soteriades
esoteria@uaeu.ac.ae
1
Jaeyoung Kim
jaeykim@dsmc.or.kr
2
Costas A Christophi
costas.christophi@cut.ac.cy
3
Stefanos N Kales
skales@hsph.harvard.edu
4
United Arab Emirates University, College of Medicine and Health Sciences, Institute of Public Health, Al Ain, United Arab Emirates.
Harvard T.H. Chan School of Public Health, Department of Environmental Health, Environmental and Occupational Medicine and Epidemiology (EOME), Boston, MA, USA.
Harvard T.H. Chan School of Public Health, Department of Environmental Health, Environmental and Occupational Medicine and Epidemiology (EOME), Boston, MA, USA.
Harvard T.H. Chan School of Public Health, Department of Environmental Health, Environmental and Occupational Medicine and Epidemiology (EOME), Boston, MA, USA.
Objective: A systematic literature review and meta-analysis was conducted on the association between firefighting and cancer. Methods: A comprehensive literature search of databases including Medline, EMBASE, Biosis, NIOSHTIC2, Web of Science, Cancerlit, and HealthStar, for the period between 1966 to January 2007, was conducted. We also retrieved additional studies by manual searching. Results: A total of 49 studies were included in the meta-analysis. We found statistically significant associations between firefighting and cancers of bladder, brain and CNS, and colorectal cancers, consistent with several previous risk estimates. We also found statistically significant associations of firefighting with non-Hodgkin’s lymphoma, skin melanoma, prostate, and testicular cancer. For kidney, Hodgkin’s lymphoma, leukemia, lymphosarcoma and reticulosarcoma, multiple myeloma, and pancreatic cancer, we found some statistically significant but less consistent results. For all other cancers evaluated (esophageal, laryngeal, oral and pharyngeal, liver and gallbladder, lung, lymphatic and hematopoietic, non-melanoma skin cancer, stomach, and urinary cancer) we did not find any statistically significant associations. Conclusions: Although our meta-analysis showed statistically significant increased risks of either cancer incidence or mortality of certain cancers in association with firefighting, a number of important limitations of the underlying studies exist, which, precluded our ability to arrive at definitive conclusions regarding causation.
https://journal.waocp.org/article_88817_5def6d405a0daaea157297e86b81980d.pdf
Cancer Incidence
mortality
Firefighters
Systematic review
Meta-analysis
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3233
3249
10.31557/APJCP.2019.20.11.3233
88805
The Lived Experience of Australian Women Living with Breast Cancer: A Meta-Synthesis
Lalithambigai Rajagopal
lalitha0211@gmail.com
1
Pranee Liamputtong
p.liamputtong@westernsydney.edu.au
2
Kate A McBride
k.mcbride@westernsydney.edu.au
3
Translational Health Research Institute, Western Sydney University, Penrith, New South Wales, Australia.
Translational Health Research Institute, Western Sydney University, Penrith, New South Wales, Australia.
Translational Health Research Institute, Western Sydney University, Penrith, New South Wales, Australia.
Background: Breast cancer is the second most common cancer among Australian women. In 2019, an estimated 19,000 women in Australia were diagnosed with breast cancer, with around 3,058 women dying from the disease in the same year. Although many qualitative studies published in Australia exist which examine breast cancer from various perspectives, only limited literature is available which addresses Australian women’s lived experience of breast cancer from diagnosis, treatment and beyond. Method: Meta-synthesis of qualitative studies. Participants who took part in either semi-structured interviews or surveys with open-ended questions were included. A thematic synthesis analysis approach was used. Results: Five themes and 13 sub themes emerged from the data analysis which illustrated the lived experience of Australian women diagnosed with breast cancer. Emotional burden and women’s response towards their breast cancer diagnosis were key themes. Experience of decision- making , social distress, symptoms beyond changes in their body, fertility considerations and their role as mothers were some of the challenges during their treatment. Women coped and adjusted with these challenges through the support of their family, and healthcare providers. Women developed greater empowerment by making their life choices after treatment. Life choices such as getting into a new relationship was challenging for single women. Conclusion: Although most women were emotionally supported following their diagnosis, there are still areas where women could be better supported such as when having to break the news of their breast cancer diagnosis to their children, provision of ongoing emotional support for caregivers of women with breast cancer, providing constant emotional and informational support at the point of diagnosis and during their treatment, tailoring treatments according to different stages of pregnancy, and discussion of fertility treatments in timely manner by healthcare professionals.
https://journal.waocp.org/article_88805_52021a2fc10f5bd49fbdab76624a4374.pdf
Breast Neoplasm
life experiences
Social Support
qualitative research
Australia
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3251
3258
10.31557/APJCP.2019.20.11.3251
88834
Neonatal Weight and Prenatal Exposure to Polychlorinated Biphenyls: A Meta-Analysis
Hongling Zou
571656246@qq.com
1
Yinxia Lin
756750162@qq.com
2
Liu Yang
18236927323@163.com
3
Chaoyan Ou
331774955@qq.com
4
Fang Geng
649116622@qq.com
5
Yao Wang
1183933276@qq.com
6
Wanrong Chen
908942503@qq.com
7
Yali Niu
1772969164@qq.com
8
Rimei Liang
815912509@qq.com
9
Qianzi Su
10
Yi Sun
sunyide163@163.com
11
Department of Oncology, Second Affiliated Hospital of Guilin Medical College, Guilin, China.
Department of Toxicology, Guilin Medical University School of Public Health, Guilin, China.
Department of Toxicology, Guilin Medical University School of Public Health, Guilin, China.
Department of Toxicology, Guilin Medical University School of Public Health, Guilin, China.
Department of Clinical Laboratory, Tangshan Maternity and Children’s Health Care Hospital, Tangshan, Hebei, China.
Department of Toxicology, Guilin Medical University School of Public Health, Guilin, China.
Department of Toxicology, Guilin Medical University School of Public Health, Guilin, China.
Department of Toxicology, Guilin Medical University School of Public Health, Guilin, China.
Department of Toxicology, Guilin Medical University School of Public Health, Guilin, China.
Department of Toxicology, Guilin Medical University School of Public Health, Guilin, China.
Department of Toxicology, Guilin Medical University School of Public Health, Guilin, China.
Background: This research studied the relationship between maternal exposure to polychlorinated biphenyls and neonatal birth weight through systematic review and meta-analysis of existing literature. Methods:We searched for all the studies published in MEDLINE / PUBMEDN / EMBASE (Medical Abstract Database) by June 2018, and seven studies had been selected. Results:The results showed that there was significant correlation between birth weight reduction and PCBS exposure throughout pregnancy (β=-0.586g, 95%CI:-0.629,-0.543). There was a negative correlation between birth weight and PCBs exposure and umbilical cord serum (β=-0.833g) and maternal serum (β= -0.504g).Subgroup analyses showed significantly different effects of PCBs exposure on birth weight in different regions, stages of pregnancy and study designs. It was thought the heterogeneity was mainly caused by geographical regions, stages of pregnancy, and the assessment methods. Conclusion: The meta analysis revealed a negative correlation between PCBs exposure and birth weight but there was significant difference in the correlation between birth weight loss.
https://journal.waocp.org/article_88834_d23e8ca4ac8f22c12c1d341c25dde82f.pdf
Polychlorinated biphenyls
Birth weight
Meta-analysis
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3259
3268
10.31557/APJCP.2019.20.11.3259
88840
Different Role of Caveolin-1 Gene in the Progression of Gynecological Tumors
Yan Gong
yyhegg@qq.com
1
Yuhan Yang
578189595@qq.com
2
Sufang Tian
usa2005tiansufang@hotmail.com
3
Honglei Chen
hl-chen@whu.edu.cn
4
Department of Pathology, Zhongnan Hospital of Wuhan University, Wuhan, P. R. China.
Department of Pathology, School of Basic Medical Science, Wuhan University, Wuhan, P. R. China.
Department of Pathology, Zhongnan Hospital of Wuhan University, Wuhan, P. R. China.
Department of Pathology, Zhongnan Hospital of Wuhan University, Wuhan, P. R. China.
Caveolin-1 (Cav-1), an integral membrane protein, is a principal component of caveolae and has been reported to play a promoting or inhibiting role in cancer progression. Gynecologic tumor is a group of tumors that affect the tissue and organs of the female reproductive system, especially cervical cancer. Cervical cancer, as one of the most common cancers, severely affects female health in developing countries in particular because of its high morbidity and mortality. This review summarizes some mechanisms of Cav-1 in the development and progression of gynecological tumors. The role of Cav-1 in tumorigenesis, including dysregulation of cell cycle, apoptosis and autophagy, adhesion, invasion, and metastasis, such as the formation of invadopodia and matrix metalloproteinase degradation are presented in detail. In addition, Cav-1 modulates autophagy and the formation of invadopodia and target regulated by miRNAs to affect tumor progress. Taken together, we find that, no matter Cav-1 expression in the tumor or stromal cells , Cav-1 has paradoxical role in different types of gynecological tumors in vivo or in vitro and even in the same tumor from the same organ.
https://journal.waocp.org/article_88840_f37256304f926112284e85da1dc04b93.pdf
caveolin-1
cancer progression
gynecological tumor
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3269
3277
10.31557/APJCP.2019.20.11.3269
88823
Prevalence of Human Papilloma Virus Sub Genotypes following Head and Neck Squamous Cell Carcinomas in Asian Continent, A Systematic Review Article
Naeem Bukhari
naeembukhari20@gmail.com
1
Joe P Joseph
2
Sayed Sajid Hussain
sajid_biotech1214@yahoo.com
3
Muhammad Adeeb Khan
adeebzoologist@hotmail.com
4
Marcel Jose Yibirin Wakim
marceljose23@gmail.com
5
Esam Bashir Yahya
essam@asmarya.edu.ly
6
Amina Arif
dr.amina@ucp.edu.pk
7
Afshan Saleem
jhonydiva71@gmail.com
8
Nadeem Sharif
shuakatali134@yahoo.com
9
Centre for Human Genetics, Hazara University Mansehra, Pakistan.
Department of Physiology and Biophysics, Case Western Reserve University School of Medicine, USA.
Department of Food Science and Engineering, University of Technology PR China.
Department of Zoology, Women University of Azad Jammu and Kashmir, Bagh, Pakistan.
Internal Medicine Department, Central University of Venezuela, Venezuela.
Department of Microbiology, Faculty of Science, Al Asmarya Islamic University, Zliten, Libya.
Faculty of Life Sciences, University of Central Punjab, Pakistan.
Department of Microbiology, University of Haripur, Pakistan.
Department of Medical Laboratory Technology, University of Haripur, Pakistan.
Objectives: In current era of blue brain intelligence and technology access at ease, standardization of disease etiology demands extensive research to drop-down human papilloma virus associated head and neck squamous cell carcinomas impact at large. Present retrospection aims to estimate comparative association of human papilloma virus sub-genotypes in head and neck squamous cell carcinomas, critical analysis of existing research gap, treatment progress, co-infection, gender association, national status and challenges following Human papilloma virus led head and neck squamous cell carcinomas among world largest continent. Background: Head and neck squamous cell carcinomas are not just like malignancies of uterine cervix, lymph nodes and breast cancers. Human papilloma virus led head and neck squamous cell carcinomas treatment directly impact Central nervous system in humans. Intriguingly, human papilloma virus mediated immune response increases patient survival, which indirectly transmit human papilloma virus in future generations and act as a potential threat developing neurogenic disorders. Methods: An objective based search strategy, following comprehensive and specific search approaches were made to retrieve recent 12 years research data from five different NCBI databases. Out of 300 shortlisted articles, only 24 principal studies met the inclusion criteria. Results: Highest human papilloma virus prevalence (10.42 %) was found in South Asia, 5.8 % in South East Asia, 5.7 % East Asia, 2.5% in west Asia and no relevant updated data was found from central Asian continent. Highest prevalence (10%) of HPV genotype-16 was recorded in Asia among 3, 710 enrolled cases including 2201 males, 1149 females and 360 cases of unknown gender. While undifferentiated multiple HPV genotype prevalence was 5.5 % (204 cases). Lowest percentage of HPV sub-types 68, 72, 57, 39 were recorded respectively. Pakistan ranked top reporting highest number of HPV-16 cases, Taiwan HPV-18, India HPV-31, Japan HPV-35 and Singapore in HPV-16 and HPV-18 co-infection rates respectively. Conclusions: Exact prevalence of HPV associated head and neck squamous cell carcinomas among Asian population is still debatable. Due to higher heterogeneity (P< 0.00001), I2 = 81-88% at 95 % confidence interval), non-availability and limitations of reported studies from Asian sub-continents especially central Asia, western Asia and from south and south east Asia demand large scale collaborative research culture to standardize head and neck squamous cell carcinomas aetiology.
https://journal.waocp.org/article_88823_31a76f0279dbec7486833b5e31e8e599.pdf
HPV
HNSCCs
South Asia
Central Asia
Middle East
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3279
3284
10.31557/APJCP.2019.20.11.3279
88811
Self- Reported Personal and Family History of Cancers in Brunei Darussalam: Result of an Integrated Health Survey
Vui Heng Chong
chongvuih@yahoo.co.uk
1
Lydiana Harliza Kadir
lydiana.abdulkadir@moh.gov.bn
2
Zakaria Kamis
hjzakaria.hjkamis@moh.gov.bn
3
Norhayati Kassim
norhayati.kassim@moh.gov.bn
4
Chee Fui Chong
wcfchong@gmail.com
5
Department of Medicine, RIPAS Hospital 2-PAPRSB Institute of Health Science, Universiti Brunei Darussalam, Brunei Darussalam.
Health Promotion Centre, Minsitry of Health, Brunei Darussalam.
Health Promotion Centre, Minsitry of Health, Brunei Darussalam.
Health Promotion Centre, Minsitry of Health, Brunei Darussalam.
Department of Surgery, RIPAS Hospital 2-PAPRSB Institute of Health Science, Universiti Brunei Darussalam, Brunei Darussalam.
Introduction: Cancers remain an important cause of mortality and morbidity, and overall incidence of cancers continues to increase worldwide with some cancers increasing while others decreasing. Understanding the epidemiology of cancer burden is important for health care planning. Most studies to date have reported incidence based on cancer registry. This aim of this study is to report the incidence of self-reported personal and family history of cancers. Materials and Methods: Data on cancers were extracted from an anonymized database of a survey (Integrated Health Screening Survey) for civil servants conducted between 2008 and 2013 (N=21,437, mean age 40.61 ± 9.46 years old, men 45.1%). Results: The overall incidence of self-reported cancers was 11.2%; personal and family histories were 0.6% and 9.4% respectively (1.2% did not state if cancers were either personal or family history). Commonly self-reported personal history of cancers were cancer of the breast, cervix and colorectal and for self-reported family history were cancers of the gastrointestinal tract, pulmonary, breast, head/neck and gynecological system. Common associations were with first degree relatives (single parent affected 50.8%, both parents affected 1.8%, siblings affected 21.9% and parents and siblings affected 3.1%). Involvement of grandparents accounted for 13.4%. The numbers affected ranged from one to three family members. For self-reported personal history of cancers, older age and gender were significant on univariate analysis and remained on multivariate analyses (p<0.05). For self–reported family history of cancers, older age, gender, professionals employment and smoking status were significant on univariate analysis but only older age, gender, race and professional employments remained significant factors on multivariate analyses (p<0.05). Conclusions: Our study showed that more than one in nine of participants reported personal or family histories of cancers, and certain characteristics were predictive of self-reporting history of cancers. Associations of cancers were mainly with first degree relatives.
https://journal.waocp.org/article_88811_c146469fbf56d27d6f487b460407c4b2.pdf
Neoplasms
Prevalence
Cancers
Malignancy
family history
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3285
3290
10.31557/APJCP.2019.20.11.3285
88812
Leaf Extracts from Dillenia philippinensis Rolfe Exhibit Cytotoxic Activity to both Drug-Sensitive and Multidrug-Resistant Cancer Cells
Rachelle Anne S Dante
rsdante1@up.edu.ph
1
Regina Joyce E Ferrer
reginajoewes@gmail.com
2
Sonia D Jacinto
soniajac2008@gmail.com
3
Institute of Biology, University of the Philippines, Diliman, Quezon City, Philippines.
Institute of Biology, University of the Philippines, Diliman, Quezon City, Philippines.
Institute of Biology, University of the Philippines, Diliman, Quezon City, Philippines.
Background: Cancer is one of the leading causes of illness and death worldwide. Only palliative therapeutic options are available for many types of cancers, and most anticancer drugs in clinical use exhibit significant side effects. It is therefore important to develop new anticancer drugs that are more effective and less toxic. In this study, we evaluate the bioactivity of a Philippine endemic plant, “katmon” or Dillenia philippinensis, and its potential use in cancer therapy. Methods: The cytotoxicity of the crude leaf extract, partitions, and isocratic column chromatography fractions of Dillenia philippinensis was determined in vitro by MTT assay against drug-sensitive cancer cell lines MCF7 (human breast adenocarcinoma) and HCT 116 (human colorectal carcinoma), as well as against moderately multidrug resistant (MDR) cancer cell line HCT-15 (human colorectal carcinoma) and its highly MDR subline HCT-15/Dox. The selectivity of the extract to cancer cells was determined by computing for the selectivity index (SI) with respect to normal mouse embryonic fibroblasts (NIH/3T3) cell line. To check for a possible mechanism for overcoming cancer multiple drug resistance, Calcein-AM assay was performed to assess the activity of the extract against P-glycoprotein-activated efflux pump. Results: Dillenia philippinensis (DP1) fraction from the hexane partition exhibited cytotoxicity (IC50< 30 µg/ml) against MCF7, HCT 116, HCT-15, and HCT-15/Dox cells. DP1 also exhibited a moderate level of selectivity against cancer cells over normal cells as supported by the SI computed from the IC50 value obtained for the normal cell line. DP1 was able to inhibit P-glycoprotein (P-gp) activity in a dose-dependent manner, suggesting its possible role in targeting cancer cells with overexpressed P-gp. Conclusion: The present findings thus demonstrate the potential chemotherapeutic properties of D. philippinensis which can be promising for future drug development against cancer.
https://journal.waocp.org/article_88812_42b263afbbb708fc9140955442339328.pdf
Dillenia philippinensis
Natural products
cancer
Multidrug Resistance
p-glycoprotein
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3291
3298
10.31557/APJCP.2019.20.11.3291
88824
Knowledge, Belief and Practice of Cervical Cancer Screening and Prevention among Women of Taraba, North-East Nigeria
Rosethe Rimande-Joel
rosetherimande@gmail.com
1
Golda Obiageri Ekenedo
golda.ekenedo@uniport.edu.ng
2
Department of Human Kinetics and Health Education, University of Port Harcourt, Nigeria.
Department of Human Kinetics and Health Education, University of Port Harcourt, Nigeria.
Objective: The rising prevalence of cancer of the cervix especially in developing countries gives cause for concern. Fortunately, it can be prevented especially when the women at risk possess correct knowledge, have the right belief and access to screening and prevention services. Previous studies have reported poor knowledge and screening practices among women in developing countries raising the fear of continued spread. The aim of the study was to establish the cervical cancer knowledge, belief and prevention/screening practices among women in Taraba, North-East Nigeria. Methods: The study adopted a cross sectional survey design. A self-designed structured and validated questionnaire with a reliability index of .82 was employed to elicit information from 978 women of child bearing age. Data collected were analysed using percentage, mean, Chi-Square and ANOVA statistics. Result: The findings of the study revealed that the women of Taraba had appropriate knowledge about the nature of cervical cancer (73. 2%) signs and symptoms of cervical cancer (76.5%) risk factors of cervical cancer (88.0%), and prevention of cervical cancer (90.3%). The women did not have the right beliefs about the concept of cervical cancer and its preventive measures. They believed that the screening procedure is painful; they were too young to contract the disease and risk stigmatization if they went for screening. Only 45.2% of the women regularly engaged in screening and other prevention practices. Location of residence, and religion significantly determined knowledge and screening/prevention practices (P<0.05) while marital status and age (P<0.05) significantly affected the respondents’ beliefs about cervical cancer and prevention practices. Location of residence, and religion significantly determined knowledge and screening/prevention practices (P<0.05) while marital status and age (P<0.05) significantly affected the respondents’ beliefs about cervical cancer and prevention practices. Conclusion: It was concluded that knowledge about cervical cancer did not translate to right belief and good practice, and that belief and practice were affected by demographic variables of location of residence, religion, marital status and age.
https://journal.waocp.org/article_88824_49a6f6e82af561d2998be0f5e06175dd.pdf
Keywords: Cervical cancer
Knowledge
beliefs
Screening
Prevention
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3299
3304
10.31557/APJCP.2019.20.11.3299
88813
Risk Factor Analysis for Breast Cancer in Premenopausal and Postmenopausal Women of Punjab, India
Akeen Kour
akeen29.k@gmail.com
1
Sarika Sharma
sarikagenetics@yahoo.com
2
Vasudha Sambyal
vasudhasambyal@yahoo.co.in
3
Kamlesh Guleria
kamleshg.human@gndu.ac.in
4
Neeti Rajan Singh
drmadhunagpal@hotmail.com
5
Manjit Singh Uppal
uppal@gmail.com
6
Mridhu Manjari
mridhuvikram@yahoo.com
7
Meena Sudan
meena_oncologist@yahoo.com
8
Sahiba Kukreja
sahibakukrja@yahoo.com
9
Human Cytogenetics Laboratory, Department of Human Genetics, Guru Nanak Dev University, Amritsar, Punjab, India.
Human Cytogenetics Laboratory, Department of Human Genetics, Guru Nanak Dev University, Amritsar, Punjab, India.
Human Cytogenetics Laboratory, Department of Human Genetics, Guru Nanak Dev University, Amritsar, Punjab, India.
Human Cytogenetics Laboratory, Department of Human Genetics, Guru Nanak Dev University, Amritsar, Punjab, India.
Department of Surgery, Sri Guru Ram Das Institute of Medical Sciences and Research, Vallah, Amritsar, Punjab, India.
Department of Surgery, Sri Guru Ram Das Institute of Medical Sciences and Research, Vallah, Amritsar, Punjab, India.
Dept of pathology, Sri Guru Ram Das Institute of Medical Sciences and Research, Vallah, Amritsar, Punjab, India.
4Department of Radiotherapy, Sri Guru Ram Das Institute of Medical Sciences and Research, Vallah, Amritsar, Punjab, India.
Department of Biochemistry, Sri Guru Ram Das Institute of Medical Sciences and Research, Vallah, Amritsar, Punjab, India.
Objective: Amritsar, the second largest town of agrarian state of Punjab, India reports high number of breast cancer cases every year. The present study investigated the etiology of breast cancer using various obesity indices and other epidemiological factors among breast cancer patients residing in and around Amritsar city. Methods: In this case control study, risk factors for breast cancer were analyzed in 542 female subjects: 271 females with breast cancer patients and 271 unrelated healthy females matched for age as control females. Results: Bivariate analysis for risk factors in cases and controls showed a lower risk (OR=0.65, 95% CI 0.43-0.99, p=0.04) in obese cases with BMI≥25kg/m2 as compared to subjects with normal BMI. Risk factor analysis showed that parameter which provided risk for cancer in postmenopausal women was obesity and in premenopausal women was parity. Postmenopausal women with BMI (overweight: OR=0.39, 95% CI 0.17-0.92, p=0.03; obese: OR= 0.26, 95% CI 0.13-0.52, p=0.00), WC (OR=0.17, 95% CI 0.05-0.52, p=0.00) and WHtR (p=0.02) had highr risk. Premenopausal women with 3 or less than 3 children had a higher risk (OR=5.54, 95 % CI 2.75-11.19, p=0.00) than postmenopausal women when compared to women with more than 3 children. Binary logistic regression analysis revealed that low parity (≤3) substantially increased the risk for breast cancer (OR=4.80, 95% CI 2.34-9.85, p=0.00) in premenopausal women. Conclusion: Obesity, parity associated breast cancer risk and reduced breastfeeding cumulatively predispose the premenopausal women of this region to higher risk of breast cancer.
https://journal.waocp.org/article_88813_e2b22fe9f792aa05cc91596d84eb436f.pdf
breast cancer
Parity
Obesity
Premenopausal
postmenopausal
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3305
3309
10.31557/APJCP.2019.20.11.3305
88828
P21 Ser31Arg and FGFR2 rs2981582 Polymorphisms as Risk Factors for Early Onset of Breast Cancer in Yogyakarta, Indonesia
Dewajani Purnomosari
d.purnomosari@ugm.ac.id
1
Clarista Raharjo
clarista.ardelia@gmail.com
2
Alvin Santoso Kalim
alvin_2495@yahoo.com
3
Rahma Herviastuti
rherviastuti@gmail.com
4
Markus Yushan
markusyushan@gmail.com
5
Rangga Athilah Fajar
ranggaaf@ymail.com
6
Karina Kazia Harris
karin_kazz@yahoo.com
7
Artanto Wahyono
artanto.wahyono@ugm.ac.id
8
Department of Histology and Cell Biology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Jl. Farmako, Sekip Utara, Yogyakarta, Indonesia.
Undergraduate Programme, Public Health and Nursing, Universitas Gadjah Mada, Jl. Farmako, Sekip Utara, Yogyakarta, Indonesia.
Undergraduate Programme, Public Health and Nursing, Universitas Gadjah Mada, Jl. Farmako, Sekip Utara, Yogyakarta, Indonesia.
Undergraduate Programme, Public Health and Nursing, Universitas Gadjah Mada, Jl. Farmako, Sekip Utara, Yogyakarta, Indonesia.
Undergraduate Programme, Public Health and Nursing, Universitas Gadjah Mada, Jl. Farmako, Sekip Utara, Yogyakarta, Indonesia.
Undergraduate Programme, Public Health and Nursing, Universitas Gadjah Mada, Jl. Farmako, Sekip Utara, Yogyakarta, Indonesia.
Undergraduate Programme, Public Health and Nursing, Universitas Gadjah Mada, Jl. Farmako, Sekip Utara, Yogyakarta, Indonesia.
Department of Surgery, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Jl. Farmako, Sekip Utara, Yogyakarta, Indonesia.
Objectives: Breast cancer tend to be more progressive with poorer prognosis in younger patients than those at an older age. Single Nucleotide Polymorphisms (SNPs) of P53 Pro72Arg, MDM2 SNP309, P21 Ser31Arg, ER SNP594, HER2 Ile655Val, and FGFR2 rs2981582 have drawn attention as genetic factors associated with cancer risk. However, there were contradictory results involving different races and their association is still unknown in Indonesian populations. This study was performed to examine the proportion of these six genes polymorphisms and their associations with age of onset of breast cancer patients in Yogyakarta, Indonesia. Methods: Biorepository DNA from 199 patients registered at Dr. Sardjito Hospital Yogyakarta from 2006-2013 were tested for polymorphisms using the PCR-RFLP method. Samples were taken from two age groups; early-onset (55 years). Chi-square tests with odds ratio were used for data analysis. Results: The mean age of the early-onset group was 36±4.2 years, while the late-onset group was 62±6.9 years. AA genotype and A allele of P21 and TT genotypes and T allele of FGFR2 were significantly more frequent and were associated with an increased risk of early-onset of breast cancer (95%CI: 2.54 and 1.59; 2.63 and 1.64, respectively). Conclusions: Our study indicates that the A allele of P21 and the allele T of FGFR2 may be associated with an increased risk of early-onset of breast cancer in Yogyakarta, Indonesia. Further analysis is needed to confirm the findings.
https://journal.waocp.org/article_88828_4c5cd5fee6bcbdc52517da9d9226e2c8.pdf
age of onset
Breast carcinoma
FGFR2
p21
Single nucleotide polymorphism
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3311
3319
10.31557/APJCP.2019.20.11.3311
88802
Explaining Perceived Priorities in Women with Breast Cancer: A Qualitative Study
Seyede Zahra Ghaemi
ghaemi.sz@yahoo.com
1
Zohreh Keshavarz
zohrehkeshavarz@protonmail.com
2
Sedigheh Tahmasebi
sedigheh.tahmasebi@yahoo.com
3
Majid Akrami
majid.akrami@yahoo.com
4
Seyed Taghi Heydari
staghi.heydari@yahoo.com
5
Student Research Committee, School of Nursing and Midwifery, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Midwifery and Reproductive Health, School of Nursing and Midwifery, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of General Surgery, Lymphedema Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Breast Diseases Research Cancer, Department of Surgery, Shiraz University of Medical Sciences, Shiraz, Iran.
Health Policy Research Center, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Objective: Cancer is a stressful event in life, and the dreadful impact and problems created for patients and families by cancer negatively affect their quality of life. Therefore, regarding the increasing number of cancer patients and the nature of this disease, the need to recognize and understand the priorities and problems of patients after the diagnosis of cancer is of high importance. This study was designed and implemented with the aim of identifying the perceived priorities of women with breast cancer. Methods: This study is a qualitative research of content analysis type. To collect data, purposeful sampling and deep semi-structured individual interviews were used. The subjects were women with breast cancer who visited the Breast Disease Research Center of Shahid Motahari Clinic in Shiraz, and the data were saturated after 15 interviews. The four criteria presented by Lincken and Guba were used to evaluate the validity and reliability. To analyze the qualitative data, conventional qualitative data analysis and MAXQDA10 software were used. Two themes were obtained in the assessment of interviews and analysis of data: 1) Supportive relief; 2) Therapeutic support. Results: Some of the participants highlighted the role of social and family support in coping with the disease, and considered social communication and continued support in the form of empathic relationship as a turning point in their lives. The absence of a fertility specialist in the cancer treatment system was the main complaint of most participants. The results showed that receiving support from family and the healthcare system is the most important perceived priorities in breast cancer patients. Conclusion: The results of this study show the importance of social support as a perceived priority in breast cancer patients to improve their quality of life. Development and reinforcement of the supportive network seem to be essential to provide the best possible help to these patients.
https://journal.waocp.org/article_88802_c4f0d2fa446234aa444283b1ae8ce7ba.pdf
Perceived Priority
Support
breast cancer
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3321
3329
10.31557/APJCP.2019.20.11.3321
88829
Down Regulation of KAI1/CD82 in Lymph Node Positive and Advanced T-Stage Group in Breast Cancer Patients
Thammineni Krishna Latha
t.krishnalatha@gmail.com
1
Ankur Verma
drankurv@gmail.com
2
Gaurav Kumar Thakur
gauravthakur110389@gmail.com
3
Basudev Banerjee
banerjeebd@hotmail.com
4
Navneet Kaur
dr_navkaur@hotmail.com
5
Usha Rani Singh
ushaucms@gmail.com
6
Sonal Sharma
sonald76@gmail.com
7
Department of Biochemistry University College of Medical Sciences and GTB Hospital , University of Delhi, Dilshad Garden, Delhi, India.
Department of Pathology, University College of Medical Sciences and GTB Hospital , University of Delhi, Dilshad Garden, Delhi, India.
Department of Biochemistry University College of Medical Sciences and GTB Hospital , University of Delhi, Dilshad Garden, Delhi, India.
Department of Biochemistry University College of Medical Sciences and GTB Hospital , University of Delhi, Dilshad Garden, Delhi, India.
Department of Surgery, University College of Medical Sciences and GTB Hospital , University of Delhi, Dilshad Garden, Delhi, India.
Department of Pathology, University College of Medical Sciences and GTB Hospital , University of Delhi, Dilshad Garden, Delhi, India.
Department of Pathology, University College of Medical Sciences and GTB Hospital , University of Delhi, Dilshad Garden, Delhi, India.
Background: Metastasis represents a deadly aspect of any cancer including breast cancer, given its high prevalence; treatment of metastatic breast cancer remains a clinically unmet need, which necessitates the exploration of metastasis suppressor genes (MSGs). KAI-1/CD82 is an important member of MSGs; the role of KAI1 has been well explored in prostate cancer, however its role in breast cancer is not fully explored and in fact the results of breast cancer studies are contentious. Thus, the present study aimed to investigate expression of KAI1 at both transcriptional and translational levels in the tissue of breast cancer patients and benign breast disease. Further, we analysed the relationship between expression levels of KAI1 and clinicopathological parameters in breast cancer patients. Materials and Methods: mRNA expression was studied by Real time PCR and protein expression was analyzed by both Western blot and Immunohistochemistry. Results: The results of the study indicate that KAI1 expression was remarkably decreased in breast cancer both at the gene and the protein levels (P < 0.05) compared to benign breast disease. In addition, KAI1 expression levels were strongly associated with axillary lymph node status and advanced T stage (p < 0.05), however no association was found with tumor grade, age, menopausal status and receptor status like ER, PR and Her2. Conclusion: Low expression of KAI1 might be helpful for predicting the lymph node metastasis and T staging, thus predicts malignant prognosis of breast cancer.
https://journal.waocp.org/article_88829_1a607b2458751d718f76a24925a4f5b2.pdf
breast cancer
Lymph node metastasis
KAI-1/CD82
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3331
3334
10.31557/APJCP.2019.20.11.3331
88836
Association between C1236T Genetic Variant of ABCB1 Gene and Molecular Response to Imatinib in Indonesian Chronic Myeloid Patients
Ikhwan Rinaldi
ikhwanrinaldi@gmail.com
1
Riki Nova
rikinovadr@gmail.com
2
Reni Widyastuti
reni.widyastuti@gmail.com
3
Rizky Priambodo
rizky.priambodo23@gmail.com
4
Instiaty Instiaty
instiaty@gmail.com
5
Melva Louisa
melva.louisa@gmail.com
6
Department of Internal Medicine, Cipto Mangunkusumo Hospital, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia.
Clinical Pharmacology Fellowship Program, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia.
Clinical Pharmacology Fellowship Program, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia.
Human Genetic Research Center, Indonesian Medical Education, and Research Institute, Jakarta Indonesia.
Department of Pharmacology and Therapeutics, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia.
Department of Pharmacology and Therapeutics, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia.
Objective: Imatinib is the first-line drug used for the treatment of chronic myeloid leukemia (CML) patients due to high molecular response and overall survival rate. However, some patients develop resistance to imatinib even after attaining a response. Mutation in ABCB1 efflux transporters is one of the known mechanisms of resistance to imatinib in chronic myeloid leukemia patients. This study was aimed to investigate the association of ABCB1 C1236T polymorphism in Indonesian chronic myeloid patients with molecular response to imatinib treatment. Methods: We analyzed 120 samples from chronic myeloid leukemia patients in the chronic phase, who had been on imatinib treatment for at least 12 months. We analyzed the C1236T variant of the ABCB1 gene using PCR, followed by direct sequencing, and associate them with the achievement of major molecular response (MMR). Results: The major molecular response was achieved in 28% of patients. The frequencies of the SNPs were CC (40%), CT (46%), and TT (14%). Our result showed that there was a lack of association between polymorphism of ABCB1 C1236T and imatinib response in Indonesian patients, with OR = 0.646 (95% CI: 0.283, 1.471; p>0.05). Conclusion: There was no association between ABCB1 C1236T variants with the major molecular response in Indonesian chronic myeloid leukemia patients receiving imatinib treatment.
https://journal.waocp.org/article_88836_6cb37852e6625546ec11fb03e44bb3b9.pdf
imatinib
ABCB1
C1236T
Chronic myeloid leukemia
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3335
3339
10.31557/APJCP.2019.20.11.3335
88839
Pharmacological Characterization and Raman Spectroscopy Evaluation of Oral and Maxillofacial Surgery-Related Carnoy´S Solution Modified by Different Viscosity Agents
Francisco Samuel Rodrigues Carvalho
samuel.rcarvalho@icloud.com
1
Marina Mota Lima Verde
marinamot@gmail.com
2
Khalil Fernandes Viana
khalil.viana@yahoo.com.br
3
Thâmara Manoela Marinho Bezerra
tmmbezerra@gmail.com
4
Said Gonçalves da Cruz Fonseca
said@ufc.br
5
Karuza Maria Alves Pereira
karuzaalves@yahoo.com.br
6
Thyciana Rodrigues Ribeiro
thyciana_odonto@yahoo.com.br
7
Fábio Wildson Gurgel Costa
fwildson@yahoo.com.br
8
Division of Oral and Maxillofacial Surgery, Federal University of Ceará Campus Sobral, Sobral, Brazil.
Dentist, UNIFOR, Fortaleza, Brazil.
Division of Oral Pathology, Federal University of Ceará, Fortaleza, Brazil.
Division of Oral Pathology, Federal University of Ceará, Fortaleza, Brazil.
Division of Pharmacology Science, Federal University of Ceará, Fortaleza, Brazil.
Department of Morphology, School of Medicine, Federal University of Ceará, Fortaleza, Brazil.
Division of Patients with Special Needs, Federal University of Ceará, Fortaleza, Brazil.
Division of Oral Radiology, Walter Cantídio University Hospital, Federal University of Ceará, Fortaleza, Brazil.
Background: There are several lesions of odontogenic and non-odontogenic origin in the oral cavity, such as odontogenic keratocyst, as well as many treatment options for such lesions. In order to reduce recurrence due to conservative treatments and less aesthetic and functional impairment of the patient (radical therapies), Carnoy’s solution has been used as an adjuvant to surgery, showing satisfactory results. Its application is not standardized, presenting risks to adjacent tissues. Thus, we characterized the Carnoy’s solution with different viscosity agents to enhance its applicability. Material and Methods: All solutions prepared (Carnoy with and without chloroform) were added with viscosity agent: ethyl cellulose, propylene glycol, and glycerol totaling eight solutions. The pharmacological characterization of the solutions was performed by determining the mass density and relative density (using a clean and dry pycnometer), pH (using pH meter), and concentration of Fe3+ (using ultraviolet/visible spectroscopy). The analyses of the inorganic components were determined by Raman micro spectrometry. Data were analyzed with statistical program BIOESTAT 5.3. Results: Solutions with ethyl cellulose were discarded due to precipitate formation and suspension of the viscosity agent. In the other solutions, viscosity increase (propylene glycol solutions) and acidic pH were observed mainly in the glycerol group. The ferric chloride characterized as a hemostatic agent had its concentration increased with the use of thickening agents, theoretically favoring its action. Conclusion: The similarity of the propylene glycol and glycerol molecules justifies the Raman spectra of these substances to be similar and the difficulty in obtaining a “fingerprint”.
https://journal.waocp.org/article_88839_7cd7b47ae4e4e122646f4d1df90ba2e2.pdf
Carnoy’s Solution
Oral Surgery
Raman Spectroscopy
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3341
3351
10.31557/APJCP.2019.20.11.3341
88825
Mdm2-P53 Interaction Inhibitor with Cisplatin Enhances Apoptosis in Colon and Prostate Cancer Cells In-Vitro
Amit Gupta
amitgupta2508@gmail.com
1
Tapan Behl
tapanbehl31@gmail.com
2
Hem Raj Heer
hm12rj65ds@rediffmail.com
3
Rahul Deshmukh
rjhddsh56erjn6@hotmail.com
4
Pyare Lal Sharma
pldfser89doc45@yahoo.com
5
Animal Tissue Culture Laboratory, Department of Pharmacology, Indo Soviet Friendship College of Pharmacy, Moga, Punjab, India.
Department of Pharmacology, Chitkara Collge of Pharmacy, Chitkara University, Rajpura, Punjab, India.
Indo Soviet Friendship College of Pharmacy, Moga, Punjab, India.
Department of Pharmaceutical Sciences and Technology, Maharaja Ranjit Singh Punjab Technical University, Bathinda, Punjab, India.
Emeritus, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
Objective: To study the effect of RITA (MDM2-p53 interaction inhibitor) and its action along with genotoxic drug cisplatin was evaluated on COLO-205 colon cancer and PC-3 prostate cancer cells. Method: Various in-vitro parameters to determine cytotoxic and apoptotic potential of RITA with genotoxic drug cisplatin were evaluated. The potentiation of cytotoxic effect was evaluated using MTT assay and colony forming assay, mechanism of cell death by Etbr/AcO assay and the mechanism of apoptosis was determined by caspase-3 release assay. Results: The findings from MTT confirmed the best possible potent combination of 5+5µM and 10+10µM concentration of Cisplatin and RITA respectively. These combinations were further evaluated for its chemo sensitizing effect which confirmed the significant reduction in number of colonies in combination as compared to monotherapy. Also, the results of Etbr/AcO assay were in line with the colony forming assay. For apoptotic activity, it was noted that increasing the concentration of cisplatin and RITA (10µM), did not affect much to apoptotic activity and was found to be equally effective to that of low dose (5µM) concentration. The same results were seen in Caspase-3 release effect on both the cell lines. Conclusion: Our present study provides compelling evidence that pharmacological activation of the p53 by blocking the MDM2–p53 interaction is a promising cancer therapeutic strategy and using RITA in combination with Cisplatin not only decrease the toxic effect of Cisplatin by decreasing its dose but also increasing the apoptotic effect, warrants clinical evaluation on both colon and prostate cancer.
https://journal.waocp.org/article_88825_e8d888b3f05bb93dab160ead27df285b.pdf
MDM2-p53 interaction
Cisplatin
Apoptosis
colon and prostate cancer cells
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3353
3359
10.31557/APJCP.2019.20.11.3353
88837
The Effectiveness of Smoking Prevention Module Towards Knowledge and Smoking Refusal Skills among Adolescents in Kota Bharu, Kelantan, Malaysia
Norlina Anuar
norlinaanuar@yahoo.com
1
Nur Suhaila Idris
nursuhaila@usm.my
2
Faridah Mohd Zin
faridahz@usm.my
3
Razlina Abdul Rahman
razlina@usm.my
4
Imran Ahmad
profimran@usm.my
5
Mohd Ismail Ibrahim
ismaildr@usm.my
6
Department of Family Medicine, School of Medical Sciences, Universiti Sains Malaysia, Kelantan, Malaysia.
Department of Family Medicine, School of Medical Sciences, Universiti Sains Malaysia, Kelantan, Malaysia.
Department of Family Medicine, School of Medical Sciences, Universiti Sains Malaysia, Kelantan, Malaysia.
Department of Family Medicine, School of Medical Sciences, Universiti Sains Malaysia, Kelantan, Malaysia.
Department of Family Medicine, School of Medical Sciences, Universiti Sains Malaysia, Kelantan, Malaysia.
Department of Community Medicine, School of Medical Sciences, Universiti Sains Malaysia, Kelantan, Malaysia.
Objective: To study the effectiveness of the smoking prevention module towards knowledge on smoking and its harmful effects and smoking refusal skills among secondary school students in Kelantan, Malaysia. Methods: A quasi experimental interventional study involving 166 non-smokers adolescents, aged 13 to 14 years old were carried out in two schools located in two different suburbs. Both schools had equal number of participants. One school was given the smoking prevention module for intervention while the control school only received the module after the study had been completed. The knowledge on smoking and its harmful effects and smoking refusal skill score were assessed using a set of validated Malay questionnaires at baseline, two weeks and eight weeks after the intervention. Repeated measure ANCOVA was used to analyse the mean score difference of both groups at baseline and after intervention. Result: Baseline analysis shows no significant difference in knowledge score between the study groups (p = 0.713) while post intervention, it shows significant inclination of knowledge score in intervention group and the difference was significant after controlling the gender [F(df) = 15.96(1.5), p <0.001]. The mean baseline for refusal skills score in the control and intervention groups were 30.89(6.164) and 28.02(6.241) respectively (p= 0.003). Post intervention, there is a significant difference in the crude mean and the estimated marginal means for smoking refusal skills score between the two groups after controlling for sex [F(df) = 5.66(1.8), p = 0.005]. Conclusion: This smoking prevention module increased the level of knowledge on smoking and its harmful effects and smoking refusal skill among the secondary school students. Thus, it is advocated to be used as one of the standard modules to improve the current method of teaching in delivering knowledge related to harmful effects of smoking and smoking refusal skill to the adolescents in Malaysia.
https://journal.waocp.org/article_88837_cb8ecd80133aafb1ac0811d43d5117dd.pdf
Smoking Prevention
Knowledge
Adolescent
Refusal to Participate
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3361
3367
10.31557/APJCP.2019.20.11.3361
88814
Up-Regulation of MiRNA-125a-5p Inhibits Cell Proliferation and Increases EGFR-TKI Induced Apoptosis in Lung Cancer Cells
Jamal Amri
jamal.amri71@gmail.com
1
Neda Molaee
n.molee91@gmail.com
2
Hadi Karami
h.karami@arakmu.ac.ir
3
Molecular and Medicine Research Center, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran.
Department of Molecular Medicine and Biotechnology, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran.
Molecular and Medicine Research Center, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran.
Background: Despite the dramatic efficacy of erlotinib, an EGFR tyrosine kinase inhibitor (TKI), most of non-small cell lung cancer (NSCLC) patients ultimately acquire resistance to this agent. Different studies indicated that miRNA-125a-5p is down-regulated in human lung cancer cells and may function as a tumor suppressor by targeting EGFR. However, the biological function of miRNA-125a-5p in NSCLC resistance to EGFR-TKIs is not fully understood. In this study the effect of miRNA-125a-5p on cell proliferation, apoptosis and sensitivity of the A549 lung cancer cells to erlotinib was investigated. Methods: After miRNA-125a-5p transfection, the expression levels of EGFR mRNA were measured by QRT-PCR. Trypan blue assays were performed to evaluate the proliferation of the A549 lung cancer cells. The cytotoxic effects of miRNA-125a-5p and erlotinib, alone and in combination, were determined using MTT assay. Combination index study was performed using the method of Chou-Talalay. Apoptosis was assessed using an ELISA cell death assay kit. Results: MiRNA-125a-5p clearly reduced the expression of EGFR mRNA in a time dependent manner, causing marked cell proliferation inhibition and spontaneous apoptosis (p<0.05, relative to control). Pretreatment with miRNA-125a-5p synergistically increased the cytotoxic effect of erlotinib and decreased its IC50. Furthermore, miRNA-125a-5p significantly enhanced the apoptotic effect of erlotinib. Negative control miRNA had no significant effect on biological parameter of the tumor cells. Conclusions: Our data suggest that suppression of EGFR by miRNA-125a-5p can effectively trigger apoptosis and overcome EGFR-TKs resistance of lung cancer cells. Therefore, miRNA-125a-5p may be a potential therapeutic adjuvant in patients with lung cancer.
https://journal.waocp.org/article_88814_b0b1b59bbc6f6f9002f5d628a95215fc.pdf
Apoptosis
EGFR
erlotinib
Lung cancer
MiRNA-125a-5p
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3369
3376
10.31557/APJCP.2019.20.11.3369
88803
The Synergistic Cytotoxic Effect of Laser-Irradiated Gold Nanoparticles and Sorafenib Against the Growth of a Human Hepatocellular Carcinoma Cell Line
Haidy M Ebrahim
haidy.morsy@nci.cu.edu.eg
1
Mahmoud N El-Rouby
mnelrouby@gmail.com
2
Mona E Morsy
monamorsy_niles@yahoo.com
3
Mahmoud M Said
mahmoudmsaid@sci.asu.edu.eg
4
Magda K Ezz
mkezz64@yahoo.com
5
Department of Cancer Biology, National Cancer Institute (NCI), Cairo University, Giza, Egypt.
Department of Cancer Biology, National Cancer Institute (NCI), Cairo University, Giza, Egypt.
Department of Medical Applications, National Institute of Laser-Enhanced Science, Cairo University, Giza, Egypt.
Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, Egypt.
Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, Egypt.
Gold nanoparticles are the most promising candidate in cancer treatment due to their physiochemical properties and increased use in photothermal therapy (PTT). In the present study, spherical gold nanoparticles (AuNPs) were synthesized using citrate reduction method. The particles were then characterized using UV-VIS spectroscopy and transmission electron microscope. A hepatocellular carcinoma cell line (HepG2) was incubated with sorafenib and/or non-irradiated or laser-irradiated AuNPs for 48 hrs. The cytotoxic effect of different treatment modalities was determined using MTT assay. Furthermore, apoptosis was determined by flow cytometry using annexin V/propidium iodide, as well as estimating the level of caspases. Results showed that AuNPs and sorafenib reduced HepG2 cell viability, and the cytotoxicity was associated with increased release of LDH in the culture medium. The recorded cytotoxicity was attributed to enhanced apoptosis as revealed by increased cellular caspases (3, 8 and 9), that was further confirmed by flow cytometry. The most notable cytotoxic effect was recorded when combining sorafenib with laser-irradiated AuNPs. In conclusion, a synergistic cytotoxic effect was observed between sorafenib and laser-irradiated AuNPs against the growth of HepG2, suggesting the potential substitution of large toxic doses of sorafenib by lower doses in combination with photothermal therapy.
https://journal.waocp.org/article_88803_b5093a34347c2acf3babf597d5d5c278.pdf
Gold Nanoparticles
sorafenib
photothermal therapy
Hepatocellular carcinoma
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3377
3383
10.31557/APJCP.2019.20.11.3377
88815
The Role of the LY294002 - A Non-Selective Inhibitor of Phosphatidylinositol 3-Kinase (PI3K) Pathway- in Cell Survival and Proliferation in Cell Line SCC-25
Andressa Duarte
andressaduarte@usp.br
1
Giórgia Gobbi Silveira
giorgia.silveira@gmail.com
2
Danilo Figueiredo Soave
danilo.patologia.oral@gmail.com
3
João Paulo Oliveira Costa
oliveiracostajp@gmail.com
4
Alfredo Ribeiro Silva
arsilva@fmrp.usp.br
5
Department of Pathology and Forensic Medicine, Ribeirão Preto Medical School, University of São Paulo, Brazil.
Department of Pathology and Forensic Medicine, Ribeirão Preto Medical School, University of São Paulo, Brazil.
Department of Pathology and Forensic Medicine, Ribeirão Preto Medical School, University of São Paulo, Brazil.
Department of Pathology and Forensic Medicine, Ribeirão Preto Medical School, University of São Paulo, Brazil.
Department of Pathology and Forensic Medicine, Ribeirão Preto Medical School, University of São Paulo, Brazil.
The activation of PI3K further activates subsequent regulatory pathways, which are activated via AKT phosphorylation. AKT is closely related to the Bcl-2 family, a protein known to be involved in cell survival. AKT also has a relationship with inflammatory and glycolytic mediators. The present work aimed to evaluate the relationship between the PI3K/AKT pathway, cell survival/proliferation, inflammatory mediators and the glycolytic pathway in oral squamous cell carcinoma. All experiments were performed in the SCC25 oral squamous cell carcinoma cell line. In the presence or absence of PI3K pathway inhibitors, we analyzed the protein expression of pAKT and AKT; X-linked inhibitor of apoptosis protein; Bcl-2-associated death promoter; Bcl-2-like protein two inhibitor; cyclooxygenase 1; cyclooxygenase-2; and glycoprotein-associated glucose transporter 1. For the functional characterization of treated or untreated cells, we also performed matrix invasion assays, cell migration assays, and cell proliferation assays. Our results demonstrated that activation of the PI3K/AKT pathway is directly related to members of the Bcl-2 family and GLUT1, but not the inflammatory mediators COX1 and COX2. Our data suggest that the PI3K/AKT pathway is related to cell survival and proliferation in oral squamous cell carcinoma through its interaction with Bcl-2 family members.
https://journal.waocp.org/article_88815_c3ebab02a5cf89d2c488de876b6f2e22.pdf
PI3K
Akt
Cox
GLUT1
Bcl-2
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3385
3389
10.31557/APJCP.2019.20.11.3385
88838
Predictive Value of Neutrophil to Lymphocyte Ratio in the Diagnosis of Significant Prostate Cancer at Initial Biopsy: A Comparison with Free Percent Prostate Specific Antigen, Prostate Specific Antigen Density and Primary Circulating Prostate Cells
Nigel P Murray
nigelpetermurray@gmail.com
1
Cynthia Fuentealba
cynthia_fuentealba_s@yahoo.es
2
Eduardo Reyes
ereyessan@gmail.com
3
Marco Antonio Lopez
marcoant.lopez@gmail.com
4
Anibal Salazar
asalazar@mi.cl
5
Simona Minzer
simona.minzer@gmail.com
6
Lorena Munoz
lorenofilix@gmail.com
7
Shenda Orrego
sp.orrego@gmail.com
8
Eghon Guzman
eguzmanm@gmail.com
9
Lucas Arzeno
lucas.arzeno@mayor.cl
10
Consultant in Hematology, Department of Medicine, Hospital de Carabineros de Chile, Simón Bolívar 2200, Ñuñoa, Santiago, Chile.
Consultant Urologist, Department of Urology, Hospital de Carabineros de Chile, Simón Bolívar, Ñuñoa, Santiago, Chile.
Consultant Urologist, Department of Urology, Hospital DIPRECA, Vital Apoquindo 1200, Las Condes, Santiago, Chile.
Physican General Medicine, Department of Medicine, Hospital de Carabineros de Chile, Simón Bolívar, Ñuñoa, Santiago, Chile.
Consultant Urologist, Department of Urology, Hospital de Carabineros de Chile, Simón Bolívar, Ñuñoa, Santiago, Chile.
Physican General Medicine, Department of Medicine, Hospital de Carabineros de Chile, Simón Bolívar, Ñuñoa, Santiago, Chile.
Consultant Internal Medicine, Department of Medicine, Hospital de Carabineros de Chile, Simón Bolívar 2200, Ñuñoa, Santiago, Chile.
Physican General Medicine, Department of Medicine, Hospital de Carabineros de Chile, Simón Bolívar, Ñuñoa, Santiago, Chile.
Physican General Medicine, Department of Medicine, Hospital de Carabineros de Chile, Simón Bolívar, Ñuñoa, Santiago, Chile.
Tutor, Faculty of Medicine, University Mayor, San Pio X 2245, Providencia, Santiago, Chile.
Introduction: An elevated serum PSA is the only biomarker routinely used in screening for prostate cancer to indicate a prostate biopsy. However, it is not specific for prostate cancer and the neutrophil/lymphocyte ratio has been suggested as an alternative. We present a prospective study of men with an elevated PSA and compare the neutrophil/lymphocyte ratio, free percent PSA, PSA density and the presence of circulating prostate cells to detect clinically significant prostate cancer at first biopsy. Patients and Methods: Prospective study of consecutive men with a PSA 4-10 ng/ml referred for initial prostate biopsy, the results were compared with the neutrophil/lymphocyte ratio, free percent PSA and PSA density. Circulating prostate cells (CPCs) were detected using immunocytochemistry. The blood sample was taken immediately before the prostate biopsy. Results: 1,223 men participated, 38% (467) of whom had prostate cancer detected, of these 322 were clinically significant. The area under the curves were for neutrophil/lymphocyte ratio, free percent PSA, PSA density and CPC detection were 0.570, 0.785, 0,620 and 0.844 respectively. Sensitivity/specificity were 0.388/0.685, 0.419/0.897, 0.598/0.624 and 0.966/0.786 respectively. The neutrophil/lymphocyte ratio did not differentiate between benign and malignant disease. Conclusions: The neutrophil/lymphocyte ratio did not discriminate between benign and malignant prostatic disease in patients with a PSA between 4-10ng/ml.
https://journal.waocp.org/article_88838_b2ee60f234b7b1d4f7c29f980f96fc46.pdf
Prostate Cancer
neutrohil/lymphocyte ratio
circulating prostate cells
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3391
3398
10.31557/APJCP.2019.20.11.3391
88804
Improved Outcomes of Childhood Acute Lymphoblastic Leukemia: A Retrospective Single Center Study in Saudi Arabia
Abdullateef Mohammed Ahmed
lateef_peds@yahoo.com
1
Hassan Al-Trabolsi
haltrabolsi@kfshrc.edu.sa
2
Mohammed Bayoumy
mbayoumi@kfshrc.edu.sa
3
Ibraheem Abosoudah
iabosoudah@kfshrc.edu.sa
4
Fawwaz Yassin
fawwazys@yahoo.com
5
King Faisal Specialist Hospital and Research Center, Jeddah Branch, Alrawdah, Jeddah, Makkah, Kingdom of Saudi Arabia.
King Faisal Specialist Hospital and Research Center, Jeddah Branch, Alrawdah, Jeddah, Makkah, Kingdom of Saudi Arabia.
King Faisal Specialist Hospital and Research Center, Jeddah Branch, Alrawdah, Jeddah, Makkah, Kingdom of Saudi Arabia.
King Faisal Specialist Hospital and Research Center, Jeddah Branch, Alrawdah, Jeddah, Makkah, Kingdom of Saudi Arabia.
King Faisal Specialist Hospital and Research Center, Jeddah Branch, Alrawdah, Jeddah, Makkah, Kingdom of Saudi Arabia.
Objective: Understanding the clinical and genetic characteristics of pediatric acute lymphoblastic leukemia (ALL) patients may help assigning the appropriate treatment. This study aims to understand patients’ characteristics, “real-world” treatment practice and outcomes of pediatric ALL. Methods: A cohort of 213 pediatric ALL patients, treated at (King Faisal Specialist Hospital and Research Center –Jeddah branch) KFSH and RC-J during the period of January 2002 to December 2015 were analyzed retrospectively. Statistical analyses were performed on patients’ demographic, clinical and genetics characteristics and outcomes of different treatment protocols. Survival was evaluated using Kaplan-Meier method, and differences in survival were tested using Log-Rank. Significance was set at 0.05 level. Results: Median age of the study cohort was 5 years (range 0.5–15 years) with 55.4% of male population. Majority of the patients had pre-B-cell ALL (88.7%), WBC count <50, 000/μL at diagnosis (76.1%, median = 13.5/μL with a range of 0.51–553.0/ μL) with involvement of central nervous system (CNS) disease in 8.5%patients.Different common chromosomal anomalies or abnormalities, including t(12, 21) translocation, MLL genre arrangements, trisomy (4, 10, 17)and others, were detected. Early response to the risk-directed treatment received by the patients (91.1% achieving <5% blast in the bone marrow) as well as the end of induction outcome (96.2%) was encouraging. Conclusion: We found that the patients’ clinical characteristics and distribution of genetic abnormalities were similar to those of the western countries. Our findings show that the earlier gap between the western countries and KSA in terms of survival has been closed and that competitive outcomes can be achieved with local infrastructure.
https://journal.waocp.org/article_88804_8e8ce9f1f5b8819f44410377efbd2d73.pdf
Acute Lymphoblastic Leukemia
Central nervous system
Chromosomal abnormalities
Cytogenetics abnormalities
Cerebrospinal fluid cytology
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3399
3406
10.31557/APJCP.2019.20.11.3399
88830
Repurposing Drugs by In Silico Methods to Target BCR Kinase Domain in Chronic Myeloid Leukemia
Aparna Natarajan
aparnan2180@gmail.com
1
Rajkumar Thangarajan
drtrajkumar@gmail.com
2
Sabitha Kesavan
rk.sabitha@gmail.com
3
Department of Molecular Oncology, Cancer Institute (WIA), Adyar, Chennai, India.
Department of Molecular Oncology, Cancer Institute (WIA), Adyar, Chennai, India.
Department of Molecular Oncology, Cancer Institute (WIA), Adyar, Chennai, India.
Background: Targeted therapy in the form of highly selective tyrosine kinase inhibitors (TKIs) has transformed the treatment of chronic myeloid leukemia (CML). However, mutations in the kinase domain contribute to drug resistance against TKIs which compromises the treatment response. Our aim is to explore regions outside the BCR-ABL oncoprotein to identify potential therapeutic targets to curb drug resistance by targeting growth factor receptor-bound protein-2 (Grb-2) which binds to BCR-ABL at the phosphorylated tyrosine (Y177) thereby activating the Ras and PI3K/AKT signaling pathway. Methods: We have used in silico methods to repurpose drugs for identifying their potential to inhibit the binding of Grb-2 with Y177 by occupying the active binding site of the BCR domain. Results: Differentially expressed genes from GEO dataset were found to be associated with hematopoietic cell lineage, NK cell-mediated cytotoxicity, NF-κB and chemokine signaling, cytokine-cytokine receptor interaction, histidine metabolism and transcriptional misregulation in cancer. The fold recognition method of SPARKS-X tool was used to model the BCR domain (Z-score = 8.21). Connectivity Map generated a drug list based on the gene expression profile, which were docked with BCR. Schrodinger XP glide docking identified Diphosphopyridine nucleotide, Hesperidin, Butirosin, Ovoflavin, and Nor-dihydroguaiaretic acid to show strong interaction in close proximity to the active binding pocket containing Y177 of the target protein and was further validated using iGEMDOCK and Parallelized Open Babel and AutoDock suite Pipeline (POAP). Conclusion: Our study not only extends our current knowledge about repurposing drugs for newer indications but also provides a route towards combinatorial therapy with standard drugs used for CML treatment. However, the efficacy of these repurposed drugs needs to be further investigated using in vitro and in vivo studies.
https://journal.waocp.org/article_88830_c3297d3972b3d955b109ac20326ea129.pdf
BCR-ABL
TKI’s
Grb-2
Tyrosine 177
computational drug repurposing
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3407
3413
10.31557/APJCP.2019.20.11.3407
88818
Comparative Analysis between the Gail, Tyrer-Cuzick and BRCAPRO Models for Breast Cancer Screening in Brazilian Population
Kely Paviani Stevanato
kelypaviani@hotmail.com
1
Raíssa Bocchi Pedroso
2
Pedro Iora
3
Lander dos Santos
lsantos@gmail.com
4
Fernando Castilho Pelloso
5
Willian Augusto de Melo
6
Maria Dalva de Barros Carvalho
7
Sandra Marisa Pelloso
8
Master's Degree in Health Sciences of the Graduate Program in Health Sciences, State University of Maringá (UEM), Brazil.
Postdoctoral Fellowship of the Postgraduate Program in Health Sciences, State University of Maringá (UEM), Brazil.
Student of the Medical School, State University of Maringá (UEM), Brazil.
Master's Degree in Health Sciences of the Graduate Program in Health Sciences, State University of Maringá (UEM), Brazil.
Student of the Medical at Federal University of Paraná (UFPR), Brazil.
Lecturer at the Nursing Department of the Paraná State University, Paranavaí campus Brazil.
Lecturer at the Department of Medicine at Unicesumar University and Professor at the Department of Postgraduate Science in Health, State University of Maringá (UEM),Brazil.
Lecturer at the Department of Medicine at Unicesumar University and Professor at the Department of Postgraduate Science in Health, State University of Maringá (UEM),Brazil.
Objective: To analyze the diagnostic accuracy of predictive models of breast cancer risk for the Brazilian population. Method: A cross-sectional, study was conducted in a sample of 382 women aged 35-69 years who were users of the Unified Health System (SUS) residing in a municipality in southern Brazil. Results: The results showed that the Tyrer-Cuzick model had the highest mean risk values and estimates (proportion) for predicting the 5-year risk of breast cancer, reaching a maximum risk of ±1.63% in the 60-64 year age group. For the 90-year risk, a maximum risk of ±12.8% was predicted for the 50-54 year age group using this model. The 5-year risk calculated by the three tools increased progressively with increasing age, where the mean risk was ±0.8% in women aged 35-39 and reached ±1.50% in women aged 65-69. The 90-year risk declined with increasing age only in the Tyrer-Cuzick model, from ±10.8% to ±9%. The BRCAPRO model presented a greater sensitivity compared to the Gail and Tyrer-Cuzick models. And, the model that presented greater specificity was Gail. Conclusion: The Tyrer-Cuzick model presented the highest risk estimates for 5 years and 90 years in the studied population, however, this data is not enough to validate this tool, since when analyzing the sensitivity and specificity the BRCAPRO and Gail have the highest values respectively.
https://journal.waocp.org/article_88818_fdc4d915cb554c899f13f7e470306df4.pdf
breast cancer
Screening
Risk models
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3415
3419
10.31557/APJCP.2019.20.11.3415
88820
Cofactors and Their Association with Cancer of the Uterine Cervix in Women Infected with High-Risk Human Papillomavirus in South India
Krishnan Baskran
drjashmi@gmail.com
1
P Kranthi Kumar
kranthi.3gbio@gmail.com
2
K Santha
santhakarunanithi@gmail.com
3
Inmozhi i Sivakamasundari
drinmozhi@gmail.com
4
Department of Biochemistry, Rajah Muthiah Medical College and Hospital, Annamalai University, Annamalainagar, Tamil Nadu, India.
Department of Genetics, Narayana Medical College & Hospital, Nellore, Andhra Pradesh, India.
Department of Biochemistry, Rajah Muthiah Medical College and Hospital, Annamalai University, Annamalainagar, Tamil Nadu, India.
Department of Biochemistry, Rajah Muthiah Medical College and Hospital, Annamalai University, Annamalainagar, Tamil Nadu, India.
Background: Human papilloma viruses (HPVs) are recognized as the major etiological agents of most pre invasive and invasive cancer of the uterine cervix. Many cofactors in association with high-risk HPV (HR-HPV) trigger infection which leads to cervical carcinogenesis. The aim was to study various cofactors and their association with cervical cancer in women infected with HR-HPV. Methods: The present study screened a total of 156 subjects for the presence of HPV infection. Association of various cofactors with cervical cancer was estimated using binary logistic regression analysis. Results: The HR-HPV infection showed a very significant risk factor for cervical cancer. Among the cofactors, the education level, early sexual exposure and age at pregnancy had no significant association while low socioeconomic status (SES) and high parity showed significant association as risk factors for cervical cancer. Tobacco chewing with betel quid was not significantly associated with cervical cancer. Conclusions: The present study indicates that low SES is a major risk factor associated with cervical cancer. Bringing awareness about HPV infection and intensifying routine screening programs for cervical cancer will help reduce the risk of cervical cancer among women with low SES in this region.
https://journal.waocp.org/article_88820_00c78906f0121cb0e36ed9652a5cf0f7.pdf
Human papilloma virus
cervical dysplasia
Cervical cancer
cofactors
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3421
3427
10.31557/APJCP.2019.20.11.3421
88819
Increased CD8 Tumor Infiltrating Lymphocytes in Colorectal Cancer Microenvironment Supports an Adaptive Immune Resistance Mechanism of PD-L1 Expression
Aru W Sudoyo
arusudoyo@gmail.com
1
Antonius N Kurniawan
ankur@indo.net.id
2
Gita D Kusumo
k.gitadewi@gmail.com
3
Teguh P Putra
teguh.pribadiputra23@gmail.com
4
Fritzie A Rexana
fritzfar@yahoo.com
5
Muhammad Yunus
muhammad.yunus.1992@gmail.com
6
Akterono D Budiyati
akterono.budiyati@kalbe.co.id
7
Dicky Kurniawan
dkurniawan.tjung@gmail.com
8
Andi Utama
andi.utama@kalbe.co.id
9
Ahmad R Utomo
ahmad.utomo@kalgenlab.com
10
Division of Hematology and Medical Oncology, Department of Internal Medicine, Universitas Indonesia, Jakarta, Indonesia.
Medistra Hospital, Jakarta, Indonesia.
Stem Cell and Cancer Institute, Jakarta, Indonesia.
Stem Cell and Cancer Institute, Jakarta, Indonesia.
Medistra Hospital, Jakarta, Indonesia.
Stem Cell and Cancer Institute, Jakarta, Indonesia.
Stem Cell and Cancer Institute, Jakarta, Indonesia.
Stem Cell and Cancer Institute, Jakarta, Indonesia.
Stem Cell and Cancer Institute, Jakarta, Indonesia.
Stem Cell and Cancer Institute, Jakarta, Indonesia.
Background: Tumor cells express programmed death ligand-1 (PD-L1) through several biological processes, thereby having different clinical significance depending on the underlying mechanism of expression. Currently, mechanisms leading to PDL1 gene expression in colorectal cancer (CRC) are not fully understood. Methods: We investigated 98 Indonesia CRC patients to determine PD-L1 protein expressions and their correlations with PD-L1 gene copy number status, tumor infiltrating lymphocytes (TILs), tumor mutational profile, as well as clinicopathologic features. Results: Our investigation demonstrated that 18% of patients positively expressed PD-L1. Further analysis on PD-L1 copy number revealed that all PD-L1+ tumors had normal copy number, indicating that the expression of PD-L1 was not a consequence of genetic amplification of PD-L1. From TILs analysis, there was a significant increase of CD8 in all tumor cells expressing PD-L1 (P=0.0051), indicating that the inducible PD-L1 expression was the prominent mechanism occurred in CRC. Furthermore, the expression of PD-L1 in this CRC population was significantly associated with high frequency of MSI compared to the remainder PD-L1- tumors (P=0.0001), suggesting the natural immunogenicity of tumors via MSI status plays role in attracting immune response. On the other hand, p53 mutations which were frequently observed within Indonesian CRCs (76.5%), they were not associated with PD-L1 expression (p=0.1108), as well as KRAS gene (29.6%; p=0.5772) and BRAF gene mutations (5%; p=0.2171). Conclusion: Our study demonstrated that PD-L1 expressions in CRC were predominantly found as a consequence of infiltrating CD8 T lymphocytes that in part arise in the setting of microsatellite instability. Taken together, our findings further support the role of adaptive immune resistance to drive PD-L1 induction in tumor microenvironment and may provide important rationale for strategy implementation of immunotherapy for CRC cases.
https://journal.waocp.org/article_88819_458ba9c5eeac12e2c7d894b74917cd63.pdf
CD8
colorectal cancer
PD-L1
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3429
3435
10.31557/APJCP.2019.20.11.3429
88826
Characterization of High-risk Oncogenic Human Papillomavirus Genotypes in Histologically Confirmed Ear, Nose and Throat (Ent) Cancers in Burkina Faso
Maïmouna Ilboudo
m.ilboudo@labiogene.org
1
Théodora Mahoukèdè Zohoncon
zohoncont1@yahoo.fr
2
Esther Mah Alima Traore
t_esther25@yahoo.fr
3
Ina Marie Angèle Traore
belima08@yahoo.fr
4
Ali Kande
kandeali@yahoo.fr
5
Florencia W Djigma
florencia.djigma@gmail.com
6
Dorcas Obiri-Yeboah
castella.oy@gmail.com
7
Yvette Marie Chantal Gyebre
ycgyebre@gmail.com
8
Jacques Simpore
jacques.simpore@yahoo.fr
9
Laboratory of Molecular Biology and Genetics (LABIOGENE), University Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
Laboratory of Molecular Biology and Genetics (LABIOGENE), University Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
Laboratory of Molecular Biology and Genetics (LABIOGENE), University Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
Laboratory of Molecular Biology and Genetics (LABIOGENE), University Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
Laboratory of Molecular Biology and Genetics (LABIOGENE), University Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
Laboratory of Molecular Biology and Genetics (LABIOGENE), University Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
University Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03; University Hospital Yalgado OUEDRAOGO Burkina Faso.
University of Cape Coast, School of Medical Sciences, Department of Microbiology and Immunology, University Post Office, Ghana.
Laboratory of Molecular Biology and Genetics (LABIOGENE), University Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
Background: High-risk human papillomavirus (HPV-HR) infections are responsible for 99.99% of cervico-uterine cancers and 50% of carcinomas of the oropharynx. Objective: To characterize high-risk HPV genotypes (HPV-HR) in histologically confirmed ear, nose and throat (ENT) cancers in Ouagadougou. Methods: One hundred and twenty-eight archived tissues from the ENT sphere, obtained over the last ten years (2007 to 2017) and histologically diagnosed in anatomy and pathology-cytology laboratories in Ouagadougou were included. These tissues were dewaxed with xylene; HPV DNA extraction was performed and HPV-HR were researched by real-time multiplex PCR. Results: Among the fourteen HPV-HR genotypes tested for, seven were identified. The prevalence of HPV-HR infection was 15.6%. The most common genotypes were: HPV56 (45%) and HPV33 (20%). Squamous cell carcinomas accounted for 75% of cases, followed by lymphomas for 10%. The age range was between 5 and 80 years. Conclusion: The results show the involvement of a diversity of HPV-HR genotypes and a high frequency of HPV56 and HPV33 in ENT cancers in Ouagadougou, Burkina Faso. The appropriate HPV vaccination will considerably reduce the number of these cancers.
https://journal.waocp.org/article_88826_15a1a90bca36017a6d3247f212dd7770.pdf
(high-risk HPV
Carcinoma
Real-Time PCR
ENT
Burkina Faso
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3437
3446
10.31557/APJCP.2019.20.11.3437
88831
An Investigation on the Therapeutic Potential of Butein, A Tretrahydroxychalcone Against Human Oral Squamous Cell Carcinoma
Devivasha Bordoloi
devivasha@iitg.ac.in
1
Javadi Monisha
j.monisha@iitg.ac.in
2
Nand Kishor Roy
r.nand@iitg.ac.in
3
Ganesan Padmavathi
padmavathi@iitg.ac.in
4
Kishore Banik
kishore.banik@iitg.ac.in
5
Choudhary Harsha
harsha.choudhary@iitg.ac.in
6
Hong Wang
snrwh@nus.edu.sg
7
Alan Prem Kumar
csiapk@nus.edu.sg
8
Frank Arfuso
frank.arfso@curtin.edu.au
9
Ajaikumar Kunnumakkara
kunnumakkara@iitg.ac.in
10
Cancer Biology Laboratory, & DAILAB, DBT-AIST International Center for Translational & Environmental Research (DAICENTER), Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Assam, India.
Cancer Biology Laboratory, & DAILAB, DBT-AIST International Center for Translational & Environmental Research (DAICENTER), Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Assam, India.
Cancer Biology Laboratory, & DAILAB, DBT-AIST International Center for Translational & Environmental Research (DAICENTER), Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Assam, India.
Cancer Biology Laboratory, & DAILAB, DBT-AIST International Center for Translational & Environmental Research (DAICENTER), Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Assam, India.
Cancer Biology Laboratory, & DAILAB, DBT-AIST International Center for Translational & Environmental Research (DAICENTER), Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Assam, India.
Cancer Biology Laboratory, & DAILAB, DBT-AIST International Center for Translational & Environmental Research (DAICENTER), Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Assam, India.
Department of Pharmacology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
Department of Pharmacology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
Stem Cell and Cancer Biology Laboratory, School of Pharmacy and Biomedical Sciences, Curtin Health Innovation Research Institute, Curtin University, Perth, WA 6102, Australia.
IIT Guwahati
Background: Oral squamous cell carcinoma (OSCC) is one of the most predominant cancers in India. With advances in the field of oncology, a number of therapies have emerged; however, they are minimally effective. Consequently, there is a need to develop safe and effective regimens for the treatment of OSCC. Butein, a tetrahydroxychalcone has been found to exhibit potent antioxidant, anti-inflammatory, and also anti-tumor effects against several cancer types. However, its effect on OSCC is not studied yet. Methods: The effect of butein on the viability, apoptosis, migration and invasion of OSCC cells was evaluated using MTT, colony formation, PI/FACS, live and dead, scratch wound healing, and matrigel invasion assays. Further Western blot analysis was done to evaluate the expression of different proteins involved in the regulation of cancer hallmarks. Results: This is the first report exemplifying the anti-cancer effect of butein against OSCC. Our results showed that butein exhibited potent anti-proliferative, cytotoxic, anti-migratory, and anti-invasive effects in OSCC cells. It suppressed the expression of NF-κB and NF-κB-regulated gene products such as COX-2, survivin and MMP-9 which are involved in the regulation of different processes like proliferation, survival, invasion, and metastasis of OSCC cells. Conclusion Collectively, these results suggest that butein has immense potential in the management of OSCC. Nonetheless, in vivo validation is critical before moving to clinical trials.
https://journal.waocp.org/article_88831_48f777df9e195fec3965d27554b15aa9.pdf
oral squamous cell carcinoma
butein
NF-KB
Proliferation
survival
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3447
3456
10.31557/APJCP.2019.20.11.3447
88806
Deep Convolution Neural Network for Malignancy Detection and Classification in Microscopic Uterine Cervix Cell Images
Shanthi P B
shanthi.moorkoth@manipal.edu
1
Faraz Faruqi
faruqi.faraz@gmail.com
2
Hareesha K S
hareesh.ks@manipal.edu
3
Ranjini Kudva
ranjini.kudva@manipal.edu
4
Department of Computer Science and Engineering, Manipal Institute of Technology, Manipal Academy of Higher Educaton, Udupi, Karnataka, India.
Department of Computer Science and Engineering, Manipal Institute of Technology, Manipal Academy of Higher Educaton, Udupi, Karnataka, India.
Department of Computer Applications, Manipal Institute of Technology, Manipal Academy of Higher Education, Udupi, Karnataka, India.
Department of Pathology, Kasturba Medical College, Manipal Academy of Higher Educaton, Udupi, Karnataka, India.
Objective: Automated Pap smear cervical screening is one of the most effective imaging based cancer detection tools used for categorizing cervical cell images as normal and abnormal. Traditional classification methods depend on hand-engineered features and show limitations in large, diverse datasets. Effective feature extraction requires an efficient image preprocessing and segmentation, which remains prominent challenge in the field of Pathology. In this paper, a deep learning concept is used for cell image classification in large datasets. Methods: This relatively proposed novel method, combines abstract and complicated representations of data acquired in a hierarchical architecture. Convolution Neural Network (CNN) learns meaningful kernels that simulate the extraction of visual features such as edges, size, shape and colors in image classification. A deep prediction model is built using such a CNN network to classify the various grades of cancer: normal, mild, moderate, severe and carcinoma. It is an effective computational model which uses multiple processing layers to learn complex features. A large dataset is prepared for this study by systematically augmenting the images in Herlev dataset. Result: Among the three sets considered for the study, the first set of single cell enhanced original images achieved an accuracy of 94.1% for 5 class, 96.2% for 4 class, 94.8% for 3 class and 95.7% for 2 class problems. The second set includes contour extracted images showed an accuracy of 92.14%, 92.9%, 94.7% and 89.9% for 5, 4, 3 and 2 class problems. The third set of binary images showed 85.07% for 5 class, 84% for 4 class, 92.07% for 3 class and highest accuracy of 99.97% for 2 class problems. Conclusion: The experimental results of the proposed model showed an effective classification of different grades of cancer in cervical cell images, exhibiting the extensive potential of deep learning in Pap smear cell image classification.
https://journal.waocp.org/article_88806_7a310c33233e0e35e7800013f70668e6.pdf
Pap smear
Cervical screening
Cell image classification
Convolution neural network
Deep Learning
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3457
3463
10.31557/APJCP.2019.20.11.3457
88827
Investigating the effect of Integrated Educational Program on the Quality of Life among Cancer Patients: A Clinical Trial Study
Maryam Bayati
bayati.ma@ajums.ac.ir
1
Shahram Molavynejad
shahrambaraz@ajums.ac.ir
2
Norollah Taheri
norollahtaheri@yahoo.com
3
Bahman Cheraghian
cheraghian2000@yahoo.com
4
Nursing Care Research Center in Chronic Diseases, School of Nursing and Midwifery, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Nursing Care Research Center in Chronic Diseases, School of Nursing and Midwifery, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Abadan Faculty of Medical Sciences, Abadan, Iran.
Department of Epidemiology and Biostatistics, School of Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Background: cancer is one of the most common causes of death around the world. The process of this disease and the resulting complications reduce the quality of life of cancer patients. Taking the necessary measures for improving the quality of life of these patients seems to be essential. This study was performed to investigate the effect of integrated educational program on the quality of life of cancer patients. Method: in this clinical trial study, 64 patients hospitalized in the specialized cancer hospital affiliated with Ahvaz Jundishapur University of Medical Sciences, Iran, were selected according to the inclusion criteria. Then, through blocked randomization method, they were assigned into intervention and control groups. The intervention group received the necessary trainings over four 60-min sessions (one session per week). The data collection in this study included demographic questionnaire and quality-of-life questionnaire of cancer patients (QLQ-C30). The quality of life was examined before the training as well as one and two months after the training. The data were analyzed by SPSS 20. Independent t-test was used to compare the means of the life quality dimensions of the studied groups. Results: all of the functional dimensions [physical , role function , emotional , cognitive , social(P≤0.05)] and symptomatic [fatigue , nausea and vomiting, pain , dyspnea , sleep disorders, diminished appetite, constipation, and diarrhea (P≤0.05)] of the quality of life of the intervention group increased significantly one and two months after running the integrated educational program. Conclusion: integrated training causes improved symptoms and enhanced quality of life in cancer patients. Thus, it is recommended that integrated training be conducted alongside the routine care of cancer patients. This can improve the therapeutic outcomes, and also highlights the important role of nurses as well as nursing cares.
https://journal.waocp.org/article_88827_14adc0eab6b7a725aa4148b207f95ed5.pdf
patient education
quality-of-life
cancer
neoplasm
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3465
3472
10.31557/APJCP.2019.20.11.3465
88807
Implication of Soluble HLA-G and HLA-G +3142G/C Polymorphism in Breast Cancer Patients Receiving Adjuvant Therapy in Tanzania
Ismael Chatita Adolf
chatitadolf@gmail.com
1
Gokce Akan
akangokce@yahoo.com
2
Teddy F Mselle
tedmselle@gmail.com
3
Nazima Dharsee
njdharsee@gmail.com
4
Lucy A Namkinga
odulajalucy@yahoo.com
5
Fatmahan Atalar
fatmahan.atalar@gmail.com
6
Department of Biochemistry, MUHAS Genetics Laboratory, Muhimbili University of Health and Allied Sciences, Tanzania.
Department of Biochemistry, MUHAS Genetics Laboratory, Muhimbili University of Health and Allied Sciences, Tanzania.
Department of Biochemistry, MUHAS Genetics Laboratory, Muhimbili University of Health and Allied Sciences, Tanzania.
Ocean Road Cancer Institute, Tanzania.
University of Dar es Salaam, Mbeya College of Health and Allied Sciences, Tanzania.
Department of Biochemistry, MUHAS Genetics Laboratory, Muhimbili University of Health and Allied Sciences, Tanzania.
Background: During cancer growth, immunosuppressive microenvironment is created that enables tumour cells to evade an eliminative immune response and hence manage to grow into malignancy. HLA-G, existing as either membrane-bound (mHLA-G) or soluble (sHLA-G) molecule is thought to be immunosuppressive and produced more by tumor cells. The +3142G/C polymorphism in HLA-G gene affects its expression, and G allele is considered to be a protective mutant allele associated with less expression of HLA-G. The implication of HLA-G in cancer development has been reported in different cancers and populations. But, its implication in most African populations has not yet been investigated. The aim of this study was to determine the possible associations of soluble HLA-G and HLA-G +3142G/C SNP with breast cancer. Materials and Methods: 75 breast cancer patients and 84 normal controls were recruited in this study. The genotyping of HLA-G +3142G/C polymorphism was determined by LightSNiP typing assay using quantitative Real-Time PCR and sHLA-G levels were determined by ELISA. Results: The sHLA-G levels were significantly lower in breast cancer patients than in controls (p<0.001). Also, they were significantly lower in mastectomized patients compared to non-mastectomized patients (p=0.018). The ROC analysis revealed a significant ability of sHLA-G to differentiate breast cancer patients versus normal controls (AUC=0.697, 95% CI= 0.619-0.767, p<0.001) and identify mastectomized patients (AUC=0.667, 95% CI= 0.549 to 0.772, p=0.041). The assessment of +3142G/C polymorphism revealed a relatively similar distribution of frequencies of genotypes and alleles between breast cancer patients and normal controls (p>0.05) and was neither associated with sHLA-G levels. Conclusion: While the +3142G/C SNP was found not to be relevant to breast cancer, the changes of sHLA-G levels in response to medical interventions such as mastectomy may be translated into its potential prognostic utility for breast cancer. More studies are needed to provide clear evidence of sHLA-G as a diagnostic and prognostic marker of breast cancer in Tanzania.
https://journal.waocp.org/article_88807_22d684c604cff3448e2f7f7e47f7c0ee.pdf
Breast cancer diagnosis
soluble HLA-G
HLA-G +3142G/C
mastectomy
Tanzanian population
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3473
3477
10.31557/APJCP.2019.20.11.3473
88816
Quality Assessment of Websites Providing Information on Human Papillomavirus Vaccines in Thailand
Naratassapol Likitdee
likitdee99@gmail.com
1
Nampet Jampathong
nampet191991@gmail.com
2
Chumnan Kietpeerakool
kiet_ji@hotmail.com
3
Department of Obstetrics and Gynaecology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Department of Obstetrics and Gynaecology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Department of Obstetrics and Gynaecology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Objectives: To assess the quality of websites that provide information about human papillomavirus (HPV) vaccination using the criteria laid out by the World Health Organization Global Advisory Committee on Vaccine Safety. Methods: This study assessed the quality of 154 Thai-language websites accessible in November 2018 via the three most popular search engines. Differences in scores were reported as mean difference (MD) and 95% confidence interval (CI). Results: With regard to credibility, all of the websites examined indicated their mission but, in most cases (91.6%), lacked transparency with regard to sponsorship. In terms of content, almost all of the websites (97.4%) met our writing/editing and content accuracy standards but did not provide complete information regarding the benefits of vaccination or adverse events associated with it. None of the websites contained information regarding their editorial/review process. All of the websites were accessible and were designed to be adaptable to mobile device screens. News and personal websites had lower credit scores than those of academic institutions (MD -0.63, 95% CI -1.05 to -0.20; MD -0.71, 95% CI -1.16 to -0.25, respectively). Conclusion: Most of the websites met standards in terms of writing/editing and content accuracy. However, fundamental information regarding the benefits and adverse events associated with HPV vaccination were infrequently reported, the editorial process and transparency issues were rarely addressed.
https://journal.waocp.org/article_88816_ca7853fc63100c477f5cf0c0664c5107.pdf
human papillomavirus
Vaccines
Quality assessment
websites
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3479
3485
10.31557/APJCP.2019.20.11.3479
88833
Association of MicroRNA 196a and 499 Polymorphisms with Development of Cirrhosis and Hepatocellular Carcinoma Post-HCV Infection in Egyptian Patients
Asmaa Mohamed Fteah
asmaa.fteah@yahoo.com
1
Asmaa Ismail Ahmed
asmaa.foraida@yahoo.com
2
Nehad Ahmed Mosaad
nanymosaad@hotmail.com
3
Mona Mohamed Hassan
dr.monahassan20@yahoo.com
4
Sherif Hamdy Mahmoud
sherifhamdy_74@hotmail.com
5
Department of Clinical and Chemical Pathology, Theodor Bilharz Research Institute, Egypt.
Department of Clinical and Chemical Pathology, Kasr Al-Ainy, Faculty of Medicine, Cairo University, Egypt.
Department of Clinical and Chemical Pathology, Kasr Al-Ainy, Faculty of Medicine, Cairo University, Egypt.
Department of Clinical and Chemical Pathology, Theodor Bilharz Research Institute, Egypt.
Department of Endemic Medicine , Liver Unit, Faculty of Medicine, Cairo University, Egypt.
Hepatocellular carcinoma (HCC) is the commonest primary tumor of the liver. Chronic HCV infection is the leading cause of end-stage liver disease, HCC and liver-related death in Egypt. Single nucleotide polymorphisms (SNPs) in microRNAs were reported to increase susceptibility to tumorigenesis; affect prognosis and as promising biomarkers in virus-host interactions. This study was conducted to investigate the role of genetic variants of miR-196a2 (rs 11614913) C>T and miR-499 (rs 3746444) A>G in the development of cirrhosis and HCC in Egyptian HCV infected patients. Genotyping of the candidate SNPs was performed by Real Time PCR in 75 HCV-related HCC patients, 75 cirrhotic patients on top of HCV and 75 healthy controls. There was significant difference in miR-499 (rs3746444) genotypes frequency between the three studied groups as the GG genotype was significantly lower in HCC cases than other groups (P = 0.009) while the combined miR-499 (AA+AG) genotypes were significantly higher in HCC cases than other groups (P = 0.005). Also a significant difference was found in miR-499 genotypes frequency when compared between HCC and cirrhosis groups as the GG genotype was significantly lower in HCC cases than cirrhosis group (P = 0.006) while the combined miR-499 (AA+AG) genotypes were significantly higher in HCC cases than in cirrhosis group (P = 0.003) [OR (95% CI) = 0.131 (0.028-0.601)]. The frequency of the G allele was significantly lower in HCC than other groups (P = 0.024) and significantly lower in HCC than normal group (P = 0.006) [OR (95%CI) = 0.501 (0.304-0.825)]. For miR-196a2 (rs11614913) C>T polymorphisms, no significant association was found with HCC risk. Our study concluded that the G allele of miR-499 is associated with lower risk of HCV related HCC development. No significant association of miR-196a2 (rs 11614913), genotypes or alleles with risk for HCC development, could be detected.
https://journal.waocp.org/article_88833_727e77881ae6b1f9dd50f56a1784cec0.pdf
Hepatocellular carcinoma
Hepatitis C Virus
MiR-196a2 C>T
MiR-499 A>G
Real Time PCR
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3487
3495
10.31557/APJCP.2019.20.11.3487
88821
Design, Synthesis and Biological Evaluation of Benzo[D]Thiazole with Dimethylpyrazol Derivatives
Dachuan Liu
dcliu1226@foxmail.com
1
Xiu Cheng
qq12345hehe@sina.com
2
Ying Wang
dcliu1226@outlook.com
3
School of Pharmacy, Bengbu Medical College, Bengbu, China.
School of Pharmacy, Bengbu Medical College, Bengbu, China.
School of Public Foundation, Bengbu Medical College, Bengbu, China.
A series of new benzothiazole derivatives containing dimethylpyrazole were synthesized and evaluated for their anticonvulsant activity, neurotoxicity and cytotoxicity by using the maximal electroshock (MES), rotarod neurotoxicity (TOX) and MTT colorimetric assay. Among the compounds studied, four compounds (6a, 6b, 6g and 6m) showed better anticonvulsant than the others at 300 mg/kg and they also showed anticonvulsant activity at the dose of 100 mg/kg. All the synthetic compounds showed lower neurotoxicity and little cytotoxicity, so that the compounds, which with better activities, also had higher protective index. In particular, the compound 6g, 2-(3,5-dimethyl-1H-pyrazol-1-yl)-6-((2-fluorobenzyl)oxy)benzo[d]thiazole showed better activity with an ED50 value of 160.4 mg/kg and higher protective index (PI) values of 2.74 in the MES test than the standard drugs sodium valproate, which used as positive controls in this study. After that the compound 6g demonstrated antagonistic activity against seizures induced by pentylenetetrazol, which proved 6g maybe exert activity through effecting GABAergic neurotransmission.
https://journal.waocp.org/article_88821_6cc22692ce6a39412c22a7e78d89de41.pdf
Benzothiazole
Dimethylpyrazol
Maximal Electroshock
MTT assay, GABAergic
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3497
3503
10.31557/APJCP.2019.20.11.3497
88808
Value of CIM, CLO Test and Multiplex PCR for the Diagnosis of Helicobacter Pylori Infection Status in Patients with Gastritis and Gastric Ulcer
Tran Thien Trung
drtranthientrung@yahoo.com
1
Tran Anh Minh
anhminhlhp@yahoo.com
2
Nguyen Tuan Anh
ntanhbio@gmail.com
3
Department of Surgery, Faculty of Medicine, University of Medicine and Pharmacy, Ho Chi Minh City, Vietnam.
Department of Surgery, Faculty of Medicine, University of Medicine and Pharmacy, Ho Chi Minh City, Vietnam.
Department of Surgery, Faculty of Medicine, University of Medicine and Pharmacy, Ho Chi Minh City, Vietnam.
Objective: To assess the value of Current Infection Marker (CIM) test, Campylobacter-Like Organism (CLO) test, and the multiplex polymerase chain reaction test (PCR) for the diagnosis of Helicobacter pylori (H. pylori) infection in a Vietnamese population. Methods: Targeted suitable patients were recruited. CIM test, CLO test and multiplex PCR were used to diagnose for H. pylori infection. Patients were considered positive for H. pylori when at least two of the three tests were positive. The performance of each of the three tests was compared to the H. pylori positive populations as defined. Result: Amongst 201 patients with a mean age of 40.5 (range, 18-74) years, there were 115 females and 86 males. Of the 201 patients, 107 (53.2%) were diagnosed as H. pylori positive according to the defined criteria. The positive patients obtained with CLO test, CIM test and multiplex PCR were 38.3%, 59.2% and 72.1%, correspondingly. The full performance of the three tests as highlighted in order as above were 85.07%, 83.08% and 81.09%, respectively. The positive rate of CLO test was the lowest, with 38.3% positive, but this method was the most accurate, with the accuracy of 85.07%. This suggested that CLO test has the highest specificity among the three. The sensitivity, specificity, positive, negative predictive values and accuracy of the CLO / CIM / multiplex PCR tests were 71.96% / 89.72% / 100%, 100% / 75.53% / 59.57%, 100% / 80.67% / 73.79%, 75.81% / 86.59% / 100%, and 85.07% / 83.08% / 81.09%, respectively. Conclusion: All the three methods have high accuracy for the diagnosis of H. pylori infection in the Vietnamese population with gastritis and gastric ulcers. These tests can be employed in the clinical settings for the Vietnamese population. CLO test should be used in combination with the other tests to reduce false-negative results.
https://journal.waocp.org/article_88808_e071bb3814043292e554b66b3e59a113.pdf
CIM test
CLO test
Helicobacter pylori
Multiplex PCR
Vietnamese
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3505
3514
10.31557/APJCP.2019.20.11.3505
88801
Nasopharyngeal Carcinoma (NPC) Risk Factors: A Systematic Review and Meta-Analysis of the Association with Lifestyle, Diets, Socioeconomic and Sociodemographic in Asian Region
Simon I Okekpa
siokekpa@student.usm.my
1
Rabiatul Basria S M N Mydin
rabiatulbasria@usm.my
2
Ernest Mangantig
e.mangantig@usm.my
3
Nor Syaffaf Amaliana Azmi
syaffaf_amaliana@yahoo.com
4
Siti Nur Syahirah Zahari
sitisyahirahah9712@gmail.com
5
Gurjeet Kaur
gurjeet@usm.my
6
Yusri Musa
myusrim@usm.my
7
Oncological and Radiological Sciences Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, 13200 Bertam, Kepala Batas, Pulau Pinang Malaysia.
Oncological and Radiological Sciences Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, 13200 Bertam, Kepala Batas, Pulau Pinang Malaysia.
Regenerative Medicine Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, 13200 Bertam, Kepala Batas, Pulau Pinang Malaysia.
Oncological and Radiological Sciences Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, 13200 Bertam, Kepala Batas, Pulau Pinang Malaysia.
Universiti Pendidikan Sultan Idris, 35900 Tanjong Malim, Perak Darul Ridzuan Malaysia.
Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Penang, Malaysia.
Oncological and Radiological Sciences Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, 13200 Bertam, Kepala Batas, Pulau Pinang Malaysia.
Objective: Risk factors of nasopharyngeal carcinoma (NPC) have been linked with diets, life style and viral infections. NPC is more rampant in Asian populations than non-Asian countries. Our study aims to assess the validity of the suggestions provided by multiple case control studies demonstrating that salted fish consumption, smoking and alcohol consumption are associated with the risk of NPC in Asia. Methods: Search for related literature on salted fish, smoking and alcohol consumption were performed via Science Direct, PubMed databases and Google Scholar. Articles included in this study were from 2009 to 2017, with specific focus on salted fish, smoking and alcohol consumption as risk factors of NPC. This study excluded all articles published prior to 2009 and articles involving other cancers. Data were extracted independently by two different researchers and harmonized. Meta-analysis was conducted on the obtained data, by using R package Meta to create funnel and forest plots. Results: The meta-analysis revealed that salted fish, smoking and alcohol consumption were significantly associated to NPC risk with random effect model score showing OR of 1.41 at 95% confidence interval (CI) of 1.13-1.75 (P<0.01), OR of 1.89 at 95 % CI of 1.49 – 2.38, and OR: 1.42 at 95 % CI of 1.23 – 1.65 respectively. Our results also revealed significant association of salted meat, salted vegetables, house type, wood dust exposure associated with NPC risk with p values less than 0.05. Conclusion: This study proposes that salted fish intake, smoking and alcohol consumption might be linked to NPC risk in Asians. Further studies are necessary to ascertain the molecular mechanisms and clarify if the associated path that could function as therapeutic target.
https://journal.waocp.org/article_88801_af0262a878c6cf3536170d3b29395070.pdf
nasopharyngeal carcinoma risk factors
Dietary cancer risk in Asian
Salted fish consumption as a cancer risk Factor
Lifestyle risk factors
Tobacco Smoking and alcohol consumption
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3515
3519
10.31557/APJCP.2019.20.11.3515
88810
Identification of Mutation in Exon11 of BRCA1 Gene in Bangladeshi Patients with Breast Cancer
Latifa Nishat
latifanishat@gmail.com
1
Zinnat Ara Yesmin
drzinnat111@gmail.com
2
Farida Arjuman
drarju35cmc@gmail.com
3
Sufi Hannan Zulfiqar Rahman
sufihannan@gmail.com
4
Laila Anjuman Banu
dr.lailabanu@gmail.com
5
Department of Anatomy, Bangabandhu Sheikh Mujib Medical University, Shahbagh, Dhaka, Bangladesh.
Department of Anatomy, Bangabandhu Sheikh Mujib Medical University, Shahbagh, Dhaka, Bangladesh.
Department of Histopathology, National Institute of Cancer Research and Hospital, Mohakhali, Dhaka, Bangladesh.
Department of Immunology and Molecular Biology, National Institute of Cancer Research and Hospital, Mohakhali, Dhaka, Bangladesh.
Department of Anatomy, Bangabandhu Sheikh Mujib Medical University, Shahbagh, Dhaka, Bangladesh.
Background: Worldwide, breast cancer is the leading cause of cancer death in female, in Bangladesh breast cancer is the second leading cancer in both sexes, and in women it occupied the top position. Highly penetrant mutations in BRCA1 gene constitute high risk of breast cancer. The spectrum of BRCA1 gene mutations varies in different population. The objective of this study was to identify mutation in exon11 of BRCA1 gene in Bangladeshi breast cancer patients. Methods: Genomic DNA was extracted from the histopathologically diagnosed formalin fixed paraffin embedded (FFPE) breast cancer tissues of 65 adult female patients. Two regions of exon11 of the BRCA1 gene were amplified and the amplicons were sequenced using Sanger sequencing. The sequenced nucleotides were analyzed and blast using NCBI nucleoblast. Selected demographic, reproductive and medical histories were collected and analyzed using SPSS version 20. Results: The mean age of the patients was 46 years and the mean age at diagnosis was 44.64 years. The patients were married and had 2.65 ± 1.22 children except one was nulliparous, the mean age of menarche was 12.67 years. All patients had children, breastfed the babies for an average 1.5 years. Only 13.6% of the patients had hypertension and the rest had no comorbidity. The family history for cancer (breast and other cancer) was negative. Three novel mutations were found in a patient. Two among the three mutant sequences had effect on amino acid coding (DNA sequence change g.852G>C and g.709G>A and amino acid changes p.Gln284His and p.Glu237Lys respectively). Conclusion: We found three novel mutations in Bangladeshi breast cancer patients. This finding indicates the necessity to study the mutation profile of whole BRCA1 gene in our population for cancer risk prediction.
https://journal.waocp.org/article_88810_d9822ccf01d734769f7944b716f6be43.pdf
exon11 mutation
BRCA1 gene
formalin fixed paraffin embedded (FFPE) breast cancer tissue
Bangladeshi breast cancer patient
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3521
3525
10.31557/APJCP.2019.20.11.3521
88809
Association between Cyclooxygenase-2 and Indoleamine 2,3-Dioxygenase Expression in Breast Cancer Patients from Pakistan
Kashif Asghar
drkashifasghar@gmail.com
1
Asif Loya
asifloya@skm.org.pk
2
Iftikhar Ali Rana
dr.ifti141@gmail.com
3
Muhammad Abu Bakar
muhammadabubakar@skm.org.pk
4
Asim Farooq
asim.farooq47302@gmail.com
5
Muhammad Tahseen
histo@skm.org.pk
6
Muhammad Ishaq
ihc@skm.org.pk
7
Muhammad Usman Rashid
usmanr@skm.org.pk
8
Department of Basic Sciences, Shaukat Khanum Memorial Cancer Hospital and Research Centre (SKMCH & RC), Lahore, Pakistan.
Department of Pathology, Shaukat Khanum Memorial Cancer Hospital and Research Centre (SKMCH & RC), Lahore, Pakistan.
Department of Pathology, Shaukat Khanum Memorial Cancer Hospital and Research Centre (SKMCH & RC), Lahore, Pakistan.
Department of Cancer Registry and Clinical Data Management, Shaukat Khanum Memorial Cancer Hospital and Research Centre (SKMCH & RC), Lahore, Pakistan.
Department of Clinical Research, Shaukat Khanum Memorial Cancer Hospital and Research Centre (SKMCH & RC), Lahore, Pakistan.
Department of Pathology, Shaukat Khanum Memorial Cancer Hospital and Research Centre (SKMCH & RC), Lahore, Pakistan.
Department of Pathology, Shaukat Khanum Memorial Cancer Hospital and Research Centre (SKMCH & RC), Lahore, Pakistan.
Department of Basic Sciences, Shaukat Khanum Memorial Cancer Hospital and Research Centre (SKMCH & RC), Lahore, Pakistan.
Background: Tumors use several immunosuppressive mechanisms to evade immune destruction. Cyclooxygenase-2 (COX-2) expression may be a driver of immunosuppression in breast cancer, but the mechanisms involved remain elusive. COX-2 expression induces the expression of indoleamine 2,3 dioxygenase (IDO) in tumor cells. IDO is an immunosuppressive enzyme which is involved in tumor immune escape mechanisms in breast cancer. Our aim was to evaluate the association between COX-2 and IDO expression to find evidence of immunosuppression in Pakistani breast cancer patients. Methods: Immunohistochemical analysis was performed to evaluate the expression of COX-2, IDO, estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER2) on formalin-fixed paraffin-embedded breast cancer tissues of 100 patients. Univariable and multivariable logistic regression model was used to identify the independent risk factors of COX-2. Results: A total of 100 patients were included with a mean age and standard deviation of 48.28 ± 11.83. A significant association was observed among COX-2, IDO, ER, PR and tumor grade. In multivariable analysis, three variables were identified as significant independent risk factors for high COX-2: IDO expression high; [adjusted odds ratio (AOR) 6.51; 95% confidence interval (CI) (2.00-21.20), p=0.001], ER; [AOR 5.62; 95% CI (1.80-17.84), p=0.002] and age [AOR 1.04; 95% CI (1.00-1.10), p=0.05] respectively. Conclusion: Our data showed that high IDO expression is associated with high COX-2 expression in Pakistani breast cancer patients. The co-expression of both enzymes may suggest their role in disease pathogenesis. Hence the concurrent targeting of COX-2 and IDO may be a promising therapy for breast cancer.
https://journal.waocp.org/article_88809_44f2505a79f9772ee2a60bf4d4dafcc2.pdf
Cyclooxygenase-2
Indoleamine 2
3-dioxygenase
breast cancer
Pakistan
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2019-11-01
20
11
3527
3531
10.31557/APJCP.2019.20.11.3527
88835
Histological Sampling of Endometrial Tissue: Comparison between the MedGyn® Endosampler and Formal Fractional Curettage in Patients with Abnormal Uterine Bleeding
Sirichoke Tumrongkunagon
minggg_13@hotmail.com
1
Wineeya Suknikhom
pearlwineeya@gmail.com
2
Department of Obstetrics and Gynecology, Phrapokklao Hospital, Thailand.
Department of Obstetrics and Gynecology, Phrapokklao Hospital, Thailand.
Background: Several methods have been used for evaluation of the endometrial pathology in patients with abnormal uterine bleeding. Endometrial biopsy is one of the primary methods used for diagnostic evaluation, which is a minimally invasive approach. The aim of this study was to compare the adequacy yield of samples obtained by MedGyn® Endosampler with Formal Fractional Curettage. Objective: This study was designed to compare the endometrial tissue quality and diagnostic accuracy between MedGyn® Endosampler and Formal Fractional Curettage in patients with abnormal uterine bleeding. Methods: A total of 85 endometrial tissue samples were tested by the MedGyn® Endosampler, followed by Formal Fractional Curettage, from patients at Phrapokklao Hospital who were eligible for the study and met the inclusion criteria for uterine curettage. Samples were collected between August, 2018 and April, 2019. Both operations were performed by the same investigator. The samples were submitted separately and sent to the same pathologist. Results: The mean age of the patients was 46.92 ± 6.94 years. 91.76% (78/85) of the samples obtained by Formal Fractional Curettage and 89.41% (76/85) of the samples obtained by MedGyn® Endosampler device were adequate for histopathological examination. The difference was not statistically significant (p = 0.317). The pathological results of endometrial tissue from both techniques were the same in 67 patients (78.82%) and different in 14 patients (16.47%). MedGyn® Endosampler was six times more cost effective when compared to Formal Fractional Curettage. Conclusions: Endometrial sampling using MedGyn® Endosampler is a safe, adequate, accurate, cost effective outpatient procedure which precludes general anesthesia. Therefore, it could be an alternative method for endometrial sampling.
https://journal.waocp.org/article_88835_f3d1fc7f609519852723e9936dbf2853.pdf
MedGyn® Endosampler
Fractional curettage
Endometrial tissue quality
Uterine bleeding