eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3133
3136
10.31557/APJCP.2020.21.11.3133
89330
Clinical Significance and Role of TK1, CEA, CA 19-9 and CA 72-4 levels in Diagnosis of Colorectal Cancers
Swarnima Singh
drswarnimasingh@outlook.com
1
Rakesh Kumar
drrakeshkumarindia@gmail.com
2
Uday Kumar
vatsuday2005@yahoo.com
3
Rekha kumari
rekhaigims1@gmail.com
4
Department of Biochemistry, Netaji Subhas Medical College and Hospital, Patna, India.
Department of Surgical Gastroenterology and Liver Transplant, Indira Gandhi Institute of Medical Sciences, Patna, India.
Department of Biochemistry, Netaji Subhas Medical College and Hospital, Patna, India.
Department of Biochemistry, Indira Gandhi Institute of Medical Sciences, Patna, India.
To explore the diagnostic value, pre-operative serum thymidine kinase 1(TK1), CEA, CA 19-9 and CA 72-4 levels were measured in 106 patients with colorectal carcinoma (53 colon and 53 rectal carcinoma patients) and 53 healthy controls. Sandwich Elisa, biotin-labeled antibody kit was used for TK1, and other tumor markers were measured using electro-chemiluminescence. Serum TK1 levels were significantly higher in CRC than in healthy controls (p <0.05) and showed significant associations with tumor stage, histopathological grade, lymph node status and metastasis (p <0.01). TK1 showed the highest (0.824-0.862) area under receiver operating characteristics curve (AUC) in comparison to other markers, and the AUC of the panel of combination tests performed even better (0.935-0.952). Significant variation was observed between the single biomarker test and their combination (Z test, p <0.01) and the Hosmer-Lemeshow test showed an adequate model of calibration. The algorithm based on combination of TK1, CEA, CA19-9 and CA72-4 can improve the diagnostic efficiency in CRC patients.
https://journal.waocp.org/article_89330_f128018f9299e297c82a238b7e2a1a70.pdf
thymidine kinase 1
colorectal cancer
Tumor markers
CEA
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3137
3144
10.31557/APJCP.2020.21.11.3137
89331
Low Socioeconomic Status Associated with Poor Cancer Screening Perceptions in Malaysia: Analysis of Determinant of Health among General Population
Shamsul Azhar Shah
drsham@ppukm.ukm.edu.my
1
Mohd Ihsani Mahmood
mmihsani@yahoo.com
2
Norfazilah Ahmad
ah.fazilah@gmail.com
3
Department of Community Health, UKM Medical Centre, Kuala Lumpur, Malaysia.
Department of Community Health, UKM Medical Centre, Kuala Lumpur, Malaysia.
Department of Community Health, UKM Medical Centre, Kuala Lumpur, Malaysia.
Background: The recent data shows reduced uptake on cancer screening where the Perception towards cancer screening by the public is one of the favorable factor might influence the screening uptake. Therefore, this study aims to determine the predictors of poor cancer screening perceptions among the population in Johor, Malaysia. Methods: This was a cross sectional study of 1,312 respondents selected using a multistage design. Questionnaires relating to the demographic characteristics, socioeconomic profiles, social and physical environment, knowledge and perception of cancer screening were gathered. Multiple logistic regression models were used to examine the variables and their association with poor perceptions of cancer screening. Results: Overall, 871(66.4%) respondents had poor perceptions of cancer screenings; 68.4% among males and 64.4% among females. In the multivariable analysis in the category of income, the bottom 40% and lower middle 40%, had not subscribed to health insurance, had poor social support, absence of any family history of cancer or comorbid illnesses, no previous attendance for cancer screening and poor knowledge of cancer, all of which were associated with their poor cancer screening perceptions. Conclusion: One way of developing cancer screening services to detect cancer in its early stage could include efforts to reach people with less awareness about cancer screening tests, lower socioeconomic status, and inadequate social support. Particular consideration should be taken to locate those who never had health insurance or attended cancer screening tests to provide the appropriate resources.
https://journal.waocp.org/article_89331_b0a25f855420448461ce77e071e29c3a.pdf
Socioeconomic status
Predictors
Cancer screening perceptions
General population
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3145
3151
10.31557/APJCP.2020.21.11.3145
89332
Human Papillomavirus and Cervical Cancer Knowledge, Perceptions, and Screening Behavior: A Cross-Sectional Community-Based Survey in Rural Philippines
Atsuko Imoto
imoto@nagasaki-u.ac.jp
1
Sumihisa Honda
honda@nagaski-u.ac.jp
2
Erlidia F Llamas-Clark
erlidia.clark@yahoo.com
3
School of Tropical Medicine and Global Health, Nagasaki University, Nagasaki, Japan.
Department of Community-based Rehabilitation Sciences, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.
Section of Ultrasound, Department of Obstetrics and Gynecology, University of Philippines Manila, Philippine General Hospital, Philippines.
Background: Cervical cancer is the second most common cancer among women in the Philippines. Cervical cancer screening is an effective method to reduce incidence. However, screening utilization is limited. This study aims to assess human papillomavirus (HPV) and cervical cancer knowledge, perceptions, and screening utilization, and to investigate factors influencing screening utilization among rural women in the Philippines. Methods: This cross-sectional community-based study was conducted among 338 rural women aged 20–50 years, with a child under 5 years old registered in one of four public rural health centers in Tacao Island, Masbate Province in October 2017. A questionnaire administered via face-to-face interviews elicited information about demographic characteristics, knowledge, perceived susceptibility and perceived severity of HPV and cervical cancer, and cervical cancer screening utilization. Results: Mean age of participants was 32.5 years. Only 13.9% of participants had ever had cervical cancer screening. Although most women had heard of cervical cancer screening, their knowledge about the cause, risk factors, and preventive measures of HPV and cervical cancer was limited. Older age and higher education status were significantly associated with screening utilization. However, knowledge and perceived susceptibility and severity showed no association. The main reason for having screening was due to a health professional’s request or recommendation, and the reasons for not having screening were cost, not having symptoms, and fear of pain or discomfort and/or embarrassment during the procedure. Conclusions: Health education must increase knowledge about HPV and cervical cancer and screening among women, including the nature and progression of cervical cancer, benefits of screening, screening cost, and screening procedure. Health care providers have an important role in educating and motivating women to undergo screening.
https://journal.waocp.org/article_89332_76e3d31df683372e1ae91c5e37114986.pdf
cervical cancer screening
Knowledge
Perception
the Philippines
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3153
3163
10.31557/APJCP.2020.21.11.3153
89333
Measurement of Serum EGFR mRNA Expression is a Reliable Predictor of Treatment Response and Survival Outcomes in Non- Small Cell Lung Cancer
Anant Mohan
anantmohan@yahoo.com
1
Ashraf Ansari
2
Mirza Masroor
3
Alpana Saxena
4
R M Pandey
5
Ashish Upadhyay
6
Kalpana Luthra
7
G C Khilnani
8
Deepali Jain
deepalijain76@gmail.com
9
Rakesh Kumar
10
Randeep Guleria
randeepguleria2002@gmail.com
11
Department of Pulmonary Medicine and Sleep Disorders, All India Institute of Medical Sciences, New Delhi, India.
Department of Pulmonary Medicine and Sleep Disorders, All India Institute of Medical Sciences, New Delhi, India.
Department of Biochemistry, Maulana Azad Medical College, New Delhi, India.
Department of Biochemistry, Maulana Azad Medical College, New Delhi, India.
Department of Biostatistics, All India Institute of Medical Sciences, New Delhi.
Department of Biostatistics, All India Institute of Medical Sciences, New Delhi.
Department of Biostatistics, All India Institute of Medical Sciences, New Delhi.
Department of Pulmonary Medicine and Sleep Disorders, All India Institute of Medical Sciences, New Delhi, India.
Department of Pathology, All India Institute of Medical Sciences, New Delhi, India.
Department of Nuclear Medicine, All India Institute of Medical Sciences, New Delhi, India.
Department of Pulmonary Medicine and Sleep Disorders, All India Institute of Medical Sciences, New Delhi, India.
Background: EGFR over-expression plays a key role in the development and progression of lung cancer. However, its status as a prognostic biomarker for survival outcomes is unclear. Objectives: To evaluate the prognostic utility of serum EGFR mRNA expression in Non-Small cell lung cancer (NSCLC) for treatment response and survival. Methods: EGFR mRNA levels were determined in serum using quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Based on ROC curve, a cut off value of 16.0-fold increase was selected to categorize patients into low EGFR (≤ 16.0) and high EGFR (> 16.0) groups. Results: A total of 350 subjects were included (78.3% males), with mean (± SD) age of 57.1 (± 11.2) years, and including 247 (70.6%) adenocarcinoma (ADC). Majority (73.1%) had metastatic (stage IV) disease. Patients had higher pre-treatment serum EGFR mRNA levels than controls [median fold-increase (min, max), 16.2 (1.9, 66.7). Serum EGFR mRNA levels significantly reduced in those who achieved objective response and disease control. Significantly longer OS and PFS was observed in subjects having baseline EGFR mRNA expression ≤ 16.0 fold- increase compared to those with > 16.0 fold- increase [median (95% CI) OS: 25.0 (14.9, NR) versus 7.7 (6.3, 8.9) months; HR (95% CI) 2.9 (2.3, 4.0), p < 0.001; and PFS: 9.9 (7.1, 11.5) versus 6.0 (4.1, 7.5) months; HR (95% CI) 1.8 (1.3, 2.4), p < 0.001]. Conclusion: Serum EGFR mRNA expression is a useful parameter for predicting treatment response and survival outcomes in NSCLC.
https://journal.waocp.org/article_89333_bc43ae1f8c4bd862ca6d35888612d015.pdf
EGFR expression
Lung cancer
survival
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3165
3170
10.31557/APJCP.2020.21.11.3165
89334
AKT1 Polymorphism (rs10138227) and Risk of Colorectal Cancer in Moroccan Population: A Case Control Study
Loubna Allam
allamloubna09@gmail.com
1
Housna Arrouchi
housnaarrouchi@gmail.com
2
Fatima Ghrifi
ghrifatima1000@gmail.com
3
Abdelhak El Khazraji
abdelhak.elkhazraji@yahoo.fr
4
Ilham Kandoussi
ilham.kandoussi@hotmail.fr
5
Mohammed Amine Bendahou
amine.bendahou@um5s.net.ma
6
Hamid El Amri
intex1000@yahoo.fr
7
Mohamed El Absi
medabssi19@gmail.com
8
Azeddine Ibrahimi
a.ibrahimi@um5s.net.ma
9
Laboratoire De Biotechnologie (MedBiotech), Faculté De Medecine Et De Pharmacie De Rabat, Université Mohamed V De Rabat, Rabat, Maroc, Morocco.
Laboratoire De Biotechnologie (MedBiotech), Faculté De Medecine Et De Pharmacie De Rabat, Université Mohamed V De Rabat, Rabat, Maroc, Morocco.
Laboratoire De Biotechnologie (MedBiotech), Faculté De Medecine Et De Pharmacie De Rabat, Université Mohamed V De Rabat, Rabat, Maroc, Morocco.
Laboratoire De Biotechnologie (MedBiotech), Faculté De Medecine Et De Pharmacie De Rabat, Université Mohamed V De Rabat, Rabat, Maroc, Morocco.
Laboratoire De Biotechnologie (MedBiotech), Faculté De Medecine Et De Pharmacie De Rabat, Université Mohamed V De Rabat, Rabat, Maroc, Morocco.
Biotechnology Laboratory (Medbiotech), Rabat Medical and Pharmacy School, Mohammed V University in Rabat, Rabat, Morroco.
Instituts Des Analyses Génétique De La Gendarmerie Royale De Rabat, Maroc, Morocco.
Faculté De Medecine Et De Pharmacie De Rabat, Université Mohamed V Rabat, Rabaat Maroc, Morocco.
Laboratoire De Biotechnologie (MedBiotech), Faculté De Medecine Et De Pharmacie De Rabat, Université Mohamed V De Rabat, Rabat, Maroc, Morocco.
Background: LMTK3 and AKT1 each have a role in carcinogenesis and tumor progression. The analysis of single nucleotide polymorphisms of AKT1 and LMTK3 could lead to more complete and accurate risk estimates for colorectal cancer. Aim: We evaluated the association between single nucleotide polymorphisms (SNPs) of AKT1 and LMTK3 and the risk of colorectal cancer in a case-control study in Moroccan population. Methods: Genomic DNA from 70 colorectal cancer patients and 50 healthy control subjects was extracted from whole blood. Genotyping was performed by direct sequencing after polymerase chain reactions for the 7 SNPs (AKT1rs1130214G/T, AKT1rs10138227C/T, AKT1rs3730358C/T, AKT1rs1000559097G/A, AKT1rs2494737A/T, LMTK3rs8108419G/A, and LMTK3rs9989661A/G.). Study subjects provided detailed information during the collection. All P values come from bilateral tests. Results: In the logistic regression analysis, a significantly high risk of colorectal cancer was associated with TC/TT genotypes of rs10138227 with adjusted odds ratio [OR] equal to 2.82 and 95% confidence interval [CI] of 1.15 to 6.91. Conclusion: Our results suggest that the SNP AKT1rs10138227 could affect susceptibility to CRC, probably by modulating the transcriptional activity of AKT1. However, larger independent studies are needed to validate our results.
https://journal.waocp.org/article_89334_3fa7f59c38e818f09a9fd4e5ca2f2121.pdf
colorectal cancer
AKT1
LMTK3
Single Nucleotide Polymorphisms
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3171
3176
10.31557/APJCP.2020.21.11.3171
89335
Effects of Quinacrine on Expression of Hippo signaling Pathway Components (LATS1, LATS2, and YAP) in Human Breast Cancer Stem Cells
Soroush Darbankhales
khales.soroush@yahoo.com
1
Reza Mirfakhraie
r.mirfakhraie@sbmu.ac.ir
2
Hossein Ghahremani
ghahremani@sbmu.ac.ir
3
Mohsen Asadolahi
m.surena@ymail.com
4
Kobra Saket-Kisomi
mahsakisom@yahoo.com
5
Lily Safakish
l.safakish@gmail.com
6
Sepideh Darbeheshti
beheshtisepid@gmail.com
7
Zahra Ganjkhanlou
zahrag3@gmail.com
8
Siamak Salami
salami.si@gmail.com
9
Majid Sirati-Sabet
sirati@sbmu.ac.ir
10
Department of Clinical Biochemistry, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Medical Genetic, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Clinical Biochemistry, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Clinical Biochemistry, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Clinical Biochemistry, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Clinical Biochemistry, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Clinical Biochemistry, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Clinical Biochemistry, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Clinical Biochemistry, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Clinical Biochemistry, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Objective: The Hippo signaling pathway has important role in the pathogenesis of some tumors. Breast cancer is the most prevalent cancer among females in the world. In recent years, various articles referred to inhibiting effect of quinacrine, a derivative of 9-aminoacridine, on the growth of several types of cancer cells. In this study, we evaluated the effect of quinacrine on expression of LATS1, LATS2, and YAP genes of the Hippo signaling pathway and YAP level in human breast cancer stem cells (MDA-MB 231 cell line). This cell line of breast cancer expresses the triple negative characteristics. Methods: MDA-MB 231 cells was treated with 0.5 µM of quinacrine for 3 days. The dose was selected using MTT assays. The expression of genes was quantified by Real-time PCR. The protein expression was performed by Western blotting. Significance of observations were checked by means of Mann-Whitney test using p
https://journal.waocp.org/article_89335_7f4b3bb9c79786f83d7f21d12cee152f.pdf
Cancer stem cells
Hippo signaling pathway
MDA-MB 231
quinacrine
triple negative breast cancer
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3177
3183
10.31557/APJCP.2020.21.11.3177
89336
Clinical Implication of Toll-Like Receptors (TLR2 and TLR4) in Acute Myeloid Leukemia Patients
Salah Aref
salaharef@yahoo.com
1
AL Shaimaa Abd Elmaksoud
alshimaa@yahoo.com
2
Sherin Abd Elaziz
sherin@yahoo.com
3
Mohamed Mabed
mabed@yahoo.com
4
Mohamed Ayed
elagder@yahoo.com
5
Hematology Unit, Department of Clinical pathology, Mansoura Faculty of Medicine, Mansoura University, Egypt.
Hematology Unit, Department of Clinical pathology, Mansoura Faculty of Medicine, Mansoura University, Egypt.
Hematology Unit, Department of Clinical pathology, Mansoura Faculty of Medicine, Mansoura University, Egypt.
Hematology Unit, Mansoura University Oncology Center, Mansoura University, Egypt.
Hematology Unit, Department of Clinical pathology, Mansoura Faculty of Medicine, Mansoura University, Egypt.
Backgrounds: Toll-like receptors 2; 4 (TLR2;4) are an essential component of the innate immunity and play an important role in immune-surveillance and immune response to various microorganisms. This study aimed to investigate the association between TLR2 and TLR4 polymorphism and the risk of acquiring severe infections, and impact on AML patient’s outcome. Subjects and methods: Using polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP); we analyzed three SNPs in the TLR2 (Arg753Gln) and TLR4 (Asp299Gly and Thr399Ile) in 120 AML patients and 100 healthy control subjects. Results: No significant differences in genotype or alleles frequency between healthy controls and AML patients regarding TLR2 Arg753Gln, TLR4 Asp299Gly and TLR4 Thr399Ile polymorphisms (P>0.05 for all). Neutropenic fever was detected in 110 out of 120 (91.7%) of the studied AML patients. The sepsis and pneumonia were identified in 20 out of 120 patients (16.7%). The incidence of sepsis was associated with TLR2 Arg753Gln: AG genotypes, A allele and TLR4 Asp299Gly: CT genotype and C allele as compared to other genotypes and alleles. Moreover; TLR2 (Arg753Gln) GG polymorphisms significantly associated with shortest overall survival (OS) and shortest disease-free survival (DFS); while TLR4 polymorphisms affect the DSF only but not OS. In AML patients TLR2 Arg753Gln gene polymorphism is associated with high susceptibility to sepsis and TLR4 (Asp299Gly and Thr399Ile) gene polymorphism is associated with high susceptibility for both pneumonia; and sepsis. Conclusion: TLR2 Arg753Gln (AG; GG genotype) polymorphisms are associated with shortest OS and DFS. Moreover; significant association between TLR2 polymorphisms, TLR4 Arg753Gln polymorphisms and risk of severe infections in AML patients was documented.
https://journal.waocp.org/article_89336_96cc2666ff998cf4216ee4f130fda2ad.pdf
TLR2
TLR4
polymorphisms
AML
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3185
3190
10.31557/APJCP.2020.21.11.3185
89337
Qualitative Study of Health Information -Seeking Barriers among Mastectomy Patients
Masoomeh Latifi
mercede_latifi@yahoo.com
1
Meghdad Sedaghat
sedaghat@sbmu.ac.ir
2
Nilofar Barahmand
nilofar_barahmand@yahoo.com
3
Fatemeh Fahimnia
fahimnia@ut.ac.ir
4
Leili Allahbakhshian Farsani
allahbakhshian@med.mui.ac.ir
5
Mother and Child Welfare Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Department of Internal Medicine, Imam Hossein Hospital, Shahid Beheshti University of medical science, Tehran, Iran.
Scientometrics Office, Vice Chancellery of Research, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Information Sciences and Knowledge Studies, Faculty of Management, Tehran University, Tehran, Iran.
Vice-Chancellery for Research and Technology, Isfahan University of Medical Sciences, Isfahan, Iran.
Background: Health information-seeking behavior (HISB) plays a key role in self-care management, promoting quality of life and improving health. However, some individual and contextual barriers hinder women undergoing mastectomy access to needed information. Identifying and removing health information-seeking barriers for these women undergoing mastectomy can lead to improving their health outcomes. Therefore, the aim of this study was to identify the health information-seeking barriers for women with breast cancer after mastectomy. Materials and Methods: This was a conventional qualitative content analysis in which the participants were selected through purposive sampling based on the study inclusion criteria from two hospitals of Shahid Mohammadi and Persian Gulf and Chemotherapy Center of Omid in Bandar Abbas. The study population consisted of 17 women with breast cancer after mastectomy. Data were collected through semi-structured face-to-face interviews. Results: Seven main themes were introduced as three individual barriers, including fear, shame and embarrassment and inadequate health literacy and four contextual barriers of economic status, physicians and medical staff, lack of accessibility of information sources and the behavior of those around them that were the underlying factors to explain the barriers of health information seeking in mastectomized women. Conclusion: The results of this study emphasize the need for further attention from Iranian authorities to health care, especially women’ health care institutions, to reform the health system and remove their health information -seeking barriers.
https://journal.waocp.org/article_89337_24b459643da65d68c88b5dffdf44cb04.pdf
health information -seeking barriers
Women
Breast Neoplasm
mastectomy
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3191
3198
10.31557/APJCP.2020.21.11.3191
89338
The Sensitivity and Specificity of Novel Primers for Detection of BRAFV600E Mutation
Bizhar Tayeb
drbat25@yahoo.com
1
Howard Pringle
jhp@le.ac.uk
2
Molecular Pathology and Toxicology, Department of Molecular Microbiology, Central Laboratory in Ibrahim Al-khalil Zakho, Duhok, Kurdistan Region of Iraq.
Department of Cancer Studies and Molecular Medicine, University of Leicester, Leicester, UK.
Objective: This study aimed to evaluate the sensitivity and specificity of a PCR-based novel technique for the detection of BRAF mutation in early stages of the cancer. Methods: Different lengths of primer sets, ranging from 8 bp to 20 bp, were designed and used in this study. These primers were developed by applying on cancer cell lines. After that, the sensitivity and specificity of the methodology was evaluated by making serial dilutions. Results: The quantitative allele specific discrimination PCR (QUASAqPCR) primer with 14 bp length was sensitive enough to detect significantly 1:1,000 ratio of BRAFV600E to wild-type background (P = 0.011), when using 150 nanograms of DNA from cell lines in the reactions. Conclusion: High sensitivity and specificity levels of QUASA-qPCR method can improve diagnostic accuracy for BRAF mutation testing in patients at early stages of cancers and help stratify the appropriate choice of treatment.
https://journal.waocp.org/article_89338_4e92a9c2433d5c8bc52b855094c5b4d6.pdf
BRAFV600E
early detection
Low abundance
Primer sensitivity
QUASA-qPCR
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3199
3209
10.31557/APJCP.2020.21.11.3199
89339
Pan-Cancer Multiomics Analysis of TC2N Gene Suggests its Important Role(s) in Tumourigenesis of Many Cancers
Muhammad Asif Qureshi
asif@asifqureshi.com
1
Saeed Khan
saeedkhn@gmail.com
2
Muhammad Sohaib Tauheed
a.qureshi@duhs.edu.pk
3
Sofia Ali Syed
sofia.ali@duhs.edu.pk
4
Ikram Din Ujjan
ikramujjan1973@yahoo.com
5
Amanullah Lail
mail2asifqureshi@yahoo.com
6
Shaheen Sharafat
shaheen.sharafat@duhs.edu.pk
7
Department of Pathology, Dow International Medical College, Dow University of Health Sciences Karachi, Pakistan.
Department of Pathology, Dow International Medical College, Dow University of Health Sciences Karachi, Pakistan.
Department of Pathology, Dow International Medical College, Dow University of Health Sciences Karachi, Pakistan.
Department of Oral Pathology, Dow Dental College, Dow University of Health Sciences, Karachi, Pakistan.
Department of Pathology, Liaquat University of Medical and Health Sciences Jamshoro, Pakistan.
Department of Paediatrics, Dow University of Health Sciences Karachi, Pakistan.
Department of Pathology, Dow International Medical College, Dow University of Health Sciences Karachi, Pakistan.
Background: Role of TC2N in carcinogenesis has been largely unfathomed until recently when it was identified as a novel oncogene in lung cancer. Subsequently, a tumour suppressor role of TC2N was reported in breast cancer. It is therefore highly relevant to investigate TC2N molecular partners/mechanisms on a larger scale including a wider range of tumour types. Methods: We investigated TC2N mRNA expression, its promoter methylation levels, effects of TC2N transcription on overall patient survival, somatic mutations in TC2N gene and correlation between TC2N mRNA expression and other cancer genes in pan-cancer by using data available from the Cancer Genome Atlas (TCGA) and the Genotype Tissue Expression (GTEx) databases. Results: TC2N mRNA expression was differentially regulated in 9/33 TCGA tumour types. Of these 9 tumours, 5 tumour types (cholangiocarcinoma, ovarian-serous-cystadenocarcinoma, rectal-adenocarcinoma, stomach-adenocarcinoma and thymoma) had significantly higher TC2N mRNA expression while 4 (pheochromocytoma-and-paraganglioma, skin-cutaneous-melanoma, thyroid-carcinoma and uterine-carcinosarcoma) had significantly lower TC2N mRNA expression compared to matched and normal controls. TC2N promoter was hypermethylated in most cancers while hypomethylated in head-and-neck-squamous-cell-carcinoma and kidney-renal-clear-cell carcinoma. TC2N transcription was positively correlated with transcription of several other cancer genes including genes from Myc, cell-cycle, Nrf2, Wnt, PI3K, Hippo, Notch, TGFβ and RAS/RTK pathways. Poor prognosis was associated with higher TC2N mRNA levels in pancreatic-adenocarcinoma and brain-lower-grade-glioma and lower TC2N mRNA levels in kidney-renal-clear-cell-carcinoma, mesothelioma, sarcoma and skin-cutaneous melanoma. Functional protein partners of TC2N were identified as STX2, SMEK1, SMEK2, STXBP5, SCARA5, MMRN1, CATSPER2, CATSPERB, CLEC4M and STAB2. Many of these proteins are key players in carcinogenesis of various cancers. Highest pathogenic somatic mutation rates in TC2N were found in skin-cutaneous-melanoma, uterine-corpus-endometrial-carcinoma, colon-endocervical-adenocarcinoma, bladder-urothelial-carcinoma and breast-invasive-carcinoma. Conclusion: Our findings unravel several un-explored avenues related to the role of TC2N in tumourigenesis of several cancers, suggesting TC2N as an important player and a potential candidate for tumour-therapy.
https://journal.waocp.org/article_89339_3f2b1807e91582ae067e71a84112c71a.pdf
TC2N
Tac2-N
TCGA
GTEx
cancer
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3211
3219
10.31557/APJCP.2020.21.11.3211
89340
Prediction of Cranial Radiotherapy Treatment in Pediatric Acute Lymphoblastic Leukemia Patients Using Machine Learning: A Case Study at MAHAK Hospital
Amirarash Kashef
amirarash.kashef@modares.ac.ir
1
Toktam Khatibi
toktamk@gmail.com
2
Azim Mehrvar
drazimmehrvar@yahoo.com
3
School of Industrial and Systems Engineering, Tarbiat Modares University (TMU), Tehran, Iran.
School of Industrial and Systems Engineering, Tarbiat Modares University (TMU), Tehran, Iran.
Mahak Hematology Oncology Research Center (Mahak-HORC), Mahak Hospital, Tehran, Iran.
Background: Acute Lymphoblastic Leukemia (ALL) is the most common blood disease in children and is responsible for the most deaths amongst children. Due to major improvements in the treatment protocols in the 50-years period, the survivability of this disease has witnessed dramatic rise until this date which is about 90 percent. There are many investigations tending to indicate the efficiency of cranial radiotherapy found out that without that, outcome of the patients did not change and even it improved at some cases. Methods: the main aim of this study is predicting cranial radiotherapy treatment in pediatric acute lymphoblastic leukemia patients using machine learning. Scope of this paper is intertwined with predicting the necessity of one of the treatment modalities that has been used for many years for this group of patients named Cranial Radiotherapy (CRT). For this purpose, a case study is considered at Mahak charity hospital. In this paper, our focus is on ALL patients aged 0 to 17 treated at Mahak hospital, one of the best centers for treatment of childhood malignancies in Iran. Dataset analyzed in this study is gathered by the research team from patient’s paper-based files. Our dataset consists of 241 observations on patients with 31 attributes after the data cleaning process. Our designed machine learning model for predicting cranial radiotherapy treatment in pediatric acute lymphoblastic leukemia patients is a stacked ensemble classifier of independently strong models with a meta-learner to tune the weights and parameters of the base classifiers. Results: The stacked ensemble classifier show highly reasonable performance with AUC of 87.52%. Moreover, the attributes are ranked based on their predictive power and the most important variable for CRT necessity prediction is the disease relapse. Conclusion: In order to conclude, derived from previous studies regarding CRT it is not only cost-effective but also more healthy to eradicate the use of CRT for the treatment of childhood ALL. Furthermore, it is valuable to increase the clinical databases by creating more synthetic health databases not only for research purposes but also for physicians to keep track of their patient’s status.
https://journal.waocp.org/article_89340_82f608c3aec6e484e3a6dd1a87f787d6.pdf
Acute lymphoblastic leukemia (ALL)
childhood blood cancer
Cranial Radiotherapy
prediction
Stacked ensemble
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3221
3227
10.31557/APJCP.2020.21.11.3221
89341
The Association of Molecular Biomarkers in the Diagnosis of Cervical Pre-Cancer and Cancer and Risk Factors in Senegalese
Dominique Diouf
dominiquediouf2002@gmail.com
1
Gora Diop
gora.diop@ucad.edu.sn
2
Cheikh Fall
cheikh.fall@pasteur.sn
3
Souleymane Sarr
essarr9477@yahoo.fr
4
Cheikh Diarra
ctdiarra94@yahoo.fr
5
Aminata Ngom
choumina87@yahoo.fr
6
Sidy Ka
sidy.ka@ucad.edu.sn
7
Seynabou Lo
zeynaby78@hotmail.com
8
Oumar Faye
doffaye@yahoo.fr
9
Ahmadou Dem
adehdem@gmail.com
10
Laboratory of Cytogenetic and Reproductive Biology, Hospital Aristide-Le-Dantec, Pasteur Avenue, Dakar, Senegal.
Cancer Studies and Research Group in Senegal (GERCS), FMPO, Cheikh Anta DIOP University, Cheikh Anta Diop Avenue, Dakar, Senegal.
Institut Pasteur Dakar, Pole de Virologie, PO Box 220. 36, Pasteur Avenue, Dakar, Senegal.
Laboratory of Anatomy and Pathology, Principal Military Hospital of Dakar, Nelson Mandela Avenue, Dakar, Senegal.
Hopital Aristide LeDantec, Service de Cancerologie, Institut-Juliot-Curie, Pasteur Avenue, PO Box 3001, Dakar, Senegal.
Laboratory of Cytogenetic and Reproductive Biology, Hospital Aristide-Le-Dantec, Pasteur Avenue, Dakar, Senegal.
Cancer Studies and Research Group in Senegal (GERCS), FMPO, Cheikh Anta DIOP University, Cheikh Anta Diop Avenue, Dakar, Senegal.
Cancer Studies and Research Group in Senegal (GERCS), FMPO, Cheikh Anta DIOP University, Cheikh Anta Diop Avenue, Dakar, Senegal.
Laboratory of Cytogenetic and Reproductive Biology, Hospital Aristide-Le-Dantec, Pasteur Avenue, Dakar, Senegal.
Cancer Studies and Research Group in Senegal (GERCS), FMPO, Cheikh Anta DIOP University, Cheikh Anta Diop Avenue, Dakar, Senegal.
Background: Cervical intraepithelial neoplasia (CIN) grading is subjective and affected by substantial rates of discordance among pathologists. Although recent studies have suggested that p16INK4a may be a useful surrogate biomarker of cervical neoplasia, Ki-67 and human papillomavirus testing have also been shown to be useful in detecting neoplasia. The purpose of this study was to determine the expression of p16INK4a and Ki-67 in cervical neoplasia and its correlations with cofactors. Methods: The study involved 69 patients with and without cervical neoplasia who underwent colposcopic directed biopsy. On each patient, two samples were taken; the first was used for immunohistochemistry and the second for molecular testing, using HPV16and18 genotyping Real-Time PCR Kit. Results: The study revealed the expression level of p16INK4a and Ki-67 in a descending order, from invasive squamous cell carcinoma (SCC), CIN2/3, CIN1 and non-dysplastic lesions. Correlations showed an association between the staining of p16NK4a and Ki-67 with the increase of age (OR: 1.79 (95%IC: 0.49 – 6.55), p = 0.037) and marital status (OR: 0.17 (95%IC: 0.04 – 0.68), p = 0.003). We found that the expressions of p16INK4a and Ki-67 were significantly different between invasive SCC vs non-dysplasia (OR: 44.57 (95%IC: 4.91 – 403.91), p <0.0001). The study showed significant correlation between HPV 16and18 infection with p16 INK4a and Ki-67 expression (OR: 0.13 (95%IC: 0.03 – 0.52), p <0.0001). Strong expression of p16INK4a and Ki-67 were observed in invasive squamous cell carcinoma, moderate staining was found in CIN2/3, weak staining in CIN1 and normal histology. Conclusion: Our findings indicate that p16INK4a and Ki-67 expressions associated strongly with cervical pathology. Therefore, p16/Ki-67 could be considered as a suitable biomarker for cervical cancer screening, particularly in HPV-based screening programs.
https://journal.waocp.org/article_89341_47500557e1c388b6a418084b23be8ab7.pdf
Biomarkers
Cervical cancer
cofactors
human papillomavirus
Senegal
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3229
3234
10.31557/APJCP.2020.21.11.3229
89342
Comparison of Polymerase Chain Reaction–Restriction Fragment Length Polymorphism, Immunohistochemistry, and DNA Sequencing for the Detection of IDH1 Mutations in Gliomas
Rusdy Malueka
rusdy_gm@ugm.ac.id
1
Emilia Theresia
emiru5286@gmail.com
2
Fitria Fitria
fitriadalimunthe@gmail.com
3
Ibnu Argo
ibnuwidyaargo@gmail.com
4
Aditya Donurizki
donurizki@gmail.com
5
Sabillal Shaleh
sabillalshaleh1312@gmail.com
6
Meutia Innayah
meutia.innayah@gmail.com
7
Adiguno Wicaksono
adiguna.sw@mail.ugm.ac.id
8
Kusumo Dananjoyo
kusumodj@gmail.com
9
Ahmad Asmedi
ahmad.asmedi@ugm.ac.id
10
Rachmat Hartanto
hartanto.rachmatandi@yahoo.com
11
Ery Kus Dwianingsih
ery_malueka@ugm.ac.id
12
Department of Neurology, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Dr. Sardjito General Hospital, Yogyakarta, Indonesia.
Department of Anatomical Pathology, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Dr. Sardjito General Hospital, Yogyakarta, Indonesia.
Department of Anatomical Pathology, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Dr. Sardjito General Hospital, Yogyakarta, Indonesia.
Department of Neurology, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Dr. Sardjito General Hospital, Yogyakarta, Indonesia.
Department of Neurology, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Dr. Sardjito General Hospital, Yogyakarta, Indonesia.
Department of Neurology, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Dr. Sardjito General Hospital, Yogyakarta, Indonesia.
Department of Neurology, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Dr. Sardjito General Hospital, Yogyakarta, Indonesia.
Division of Neurosurgery, Department of Surgery, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Dr. Sardjito General Hospital, Yogyakarta, Indonesia.
Department of Neurology, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Dr. Sardjito General Hospital, Yogyakarta, Indonesia.
Department of Neurology, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Dr. Sardjito General Hospital, Yogyakarta, Indonesia.
Division of Neurosurgery, Department of Surgery, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Dr. Sardjito General Hospital, Yogyakarta, Indonesia.
Department of Anatomical Pathology, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Dr. Sardjito General Hospital, Yogyakarta, Indonesia.
Background: IDH1 mutation shows diagnostic, prognostic, and predictive value in gliomas. Direct Sanger sequencing is considered the gold standard to detect IDH1 mutation. However, this technology is not available in most neuropathological centers in developing countries such as Indonesia. Immunohistochemistry (IHC) and polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) have also been used to detect IDH1 mutation. This study aimed to compare DNA sequencing, IHC, and PCR–RFLP in detecting IDH1 mutations in gliomas. Methods: Research subjects were recruited from Dr. Sardjito Hospital. Genomic DNA was extracted from fresh or formalin-fixed paraffin-embedded samples of tumor tissue. DNA sequencing, PCR–RFLP and IHC were performed to detect IDH1 mutation. Sensitivity, specificity, and accuracy of PCR–RFLP and IHC were calculated by comparing them to DNA sequencing as the gold standard. Results: Among 61 recruited patients, 13 (21.3%) of them carried a mutation in codon 132 of the IDH1 gene, as shown by DNA sequencing. PCR–RFLP and DNA sequencing have a concordance value of 100%. Meanwhile, the concordance value between IDH1 R132H IHC and DNA sequencing was 96.7%. The sensitivity, specificity, positive predictive values, negative predictive values, and accuracy for PCR–RFLP were all 100%. On the other hand, the sensitivity, specificity, and accuracy of IHC were 92.3%, 97.9%, and 96.7%, respectively. Conclusion: This study showed that both PCR–RFLP and IHC have high accuracy in detecting IDH1 mutation. We recommend a combination of PCR–RFLP and IHC to detect IDH1 mutation in resource-limited settings.
https://journal.waocp.org/article_89342_4655126cee87055d5877d5d77dd1d67f.pdf
Glioma
IDH1 gene
DNA Sequencing
PCR–RFLP
immunuhistochemistry
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3235
3240
10.31557/APJCP.2020.21.11.3235
89343
Estrogen Receptor 1 Gene (ESR1) rs2234693 Polymorphism and Breast Cancer Risk in Saudi Women
Razan Al-Amri
zeenh_2010@hotmail.com
1
Mohammad Alotibi
mkotaibi@taibahu.edu.sa
2
Rawya AL-Raddadi
rawya19921@outlook.sa
3
Weam Shebli
weam92@outlook.sa
4
Emad Fallatah
dr.fallatah33@gmail.com
5
Ahmed Alhujaily
ans1420@hotmail.com
6
Hiba Mohamed
hmohamedahmed@taibahu.edu.sa
7
Department of Biology, College of Science, Taibah University, Madinah, Kingdom of Saudi.
Department of Biology, College of Science, Taibah University, Madinah, Kingdom of Saudi.
Department of Biology, College of Science, Taibah University, Madinah, Kingdom of Saudi.
Department of Biology, College of Science, Taibah University, Madinah, Kingdom of Saudi.
Department of Oncology, King Fahad Hospital, Madinah, Kingdom of Saudi Arabia.
Department of Pathology, King Fahad Hospital, Madinah, Kingdom of Saudi Arabia.
Department of Biology, College of Science, Taibah University, Madinah, Kingdom of Saudi.
Objective: The present study aimed to determine the role of ESR1 gene rs2234693 T/C polymorphism (PvuII) in the susceptibility to breast cancer and to assess the association of this polymorphism within presence or absence of estrogen, progesterone receptors, human epidermal growth factor receptor 2 (HER2) and with premenopausal and postmenopausal age in Saudi women. Methods: The study was a retrospective case-control study. In this study, 137 breast cancer and 98 normal breast paraffin embedded tissues were included. DNA was extracted and ESR1 gene rs2234693 T/C polymorphism was genotyped by PCR-RFLP. Genetic association tests were performed. Results: The results showed no significant difference in distribution of rs2234693 T/C alleles and genotypes frequencies. Odd ratios (95% CI) were 1.15 (0.8-1.66) and 1.06 (0.5-1.98) and p values were 0.51 and 0.87, respectively. The genotypes and alleles frequencies within different hormonal receptors groups and ages of menopause showed no signification association (odd ratios were less or close to 1 and p values > 0.05). Conclusion: ESR1 gene rs2234693 T/C polymorphism was not associated with susceptibility to breast cancer and different menopausal, hormone receptors, and HER2 status in breast cancer patients. Further analysis using larger sample size will be needed to assess the association of different polymorphisms within the gene and risk of breast cancer.
https://journal.waocp.org/article_89343_00a512cb2bb0e17576bab37970e3433a.pdf
genetic variant
ESR1
breast cancer
Saudi Arabia
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3241
3250
10.31557/APJCP.2020.21.11.3241
89344
Differences in Cancer Mortality Trends between Metropolitan and Non-Metropolitan Areas in Japan, 1999–2018
Tasuku Okui
task10300@gmail.com
1
Medical Information Center, Kyushu University Hospital, Fukuoka city, Japan.
Background: Although socioeconomic statuses affect cancer mortality rates, the specific difference between metropolitan and non-metropolitan areas in Japan has not been evaluated. This study analyzed differences in cancer mortality between metropolitan and non-metropolitan areas in Japan, using an age-period-cohort (APC) analysis. Methods: Data on cancer mortality from 1999 to 2018 for metropolitan and non-metropolitan areas in Japan were used. Here metropolitan areas were defined as government ordinance-designated municipalities in 1999 and special wards of Tokyo. In addition to general mortality data for all cancer sites, data on mortality for stomach, colorectal, liver, gallbladder, pancreatic, lung, prostate, and breast cancers were used for analysis. A Bayesian APC analysis was administered to the data for each type of cancer for area and for sex-distinguished data. Additionally, the ratios for estimated mortality rate by periods and cohorts between the two areas were calculated. Results: The age-standardized mortality rate for cancer in all sites in non-metropolitan areas was lower than that in metropolitan areas throughout the analyzed years for both men and women, but the mortality difference decreased during the periods for men. The rates of decrease in mortality rate in cohorts differed for some cancers between the two area types, and the mortality rate ratios of metropolitan compared with non-metropolitan areas decreased for cancer in all sites over the analyzed cohorts for men. Also, the rate of decrease in mortality rate over the cohorts was completely different between the areas for stomach cancer in men and for liver cancer for women. Conclusion: Mortality rates for cancer in all sites tended to diverge between the two area types in younger cohorts for men, and people in younger cohorts in non-metropolitan areas should take more extensive preventive measures against cancer than their counterparts in metropolitan areas.
https://journal.waocp.org/article_89344_864e8864f8a09aeb7665c8205cfec7c5.pdf
health status disparities
vital statistics
mortality
cancer
Cohort effects
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3251
3258
10.31557/APJCP.2020.21.11.3251
89345
Karachi Cancer Registry (KCR): Age-Standardized Incidence Rate by Age-Group and Gender in a Mega City of Pakistan
Shahid Pervez
shahidpervez@aku.edu
1
Adnan A Jabbar
adnan.jabbar@aku.edu
2
Ghulam Haider
dr_ghaider1@yahoo.com
3
Shamvil Ashraf
shamvil.ashraf@tih.org.pk
4
Muhammad Qureshi
qureshi@duhs.edu.pk
5
Fouzia Lateef
fouzia_ky@yahoo.com
6
Imtiaz Bashir
imtiaz@panjwani.org
7
Manzoor Zaidi
bionco@cyber.net.pk
8
Mohammad Khurshid
mohammad.khurshid@aku.edu
9
Mohammad Quraishy
saeed.quraishy@duhs.edu.pk
10
Tariq Siddiqi
tsidmd@hotmail.com
11
Uzma Rizwan
uzma.rizwan@gmail.com
12
Muhammad Arif Saqib
arif289@gmail.com
13
Muhammad Memon
alimemon2k@yahoo.com
14
Ejaz Alam
eazmu2004@yahoo.com
15
Huma Qureshi
drhumapmrc@gmail.com
16
Department of Pathology & Laboratory Medicine, Aga Khan University Hospital, Karachi, Pakistan.
Department of Pathology & Laboratory Medicine, Aga Khan University Hospital, Karachi, Pakistan.
Jinnah Postgraduate Medical Centre, Karachi, Pakistan.
National Institute of Child Health JPMC, Karachi, Pakistan.
Dow University of Health Sciences, Karachi, Pakistan.
Ziauddin University Hospital, Karachi, Pakistan.
Zainab Punjwani Hospital, Karachi, Pakistan.
Baqai Medical University Hospital, Karachi, Pakistan.
Department of Pathology & Laboratory Medicine, Aga Khan University Hospital, Karachi, Pakistan.
Dow University of Health Sciences, Karachi, Pakistan.
Ziauddin University Hospital, Karachi, Pakistan.
Care Point Health System New Jersey, USA.
Pakistan Health Research Council Research Centre, Karachi, Pakistan.
Atomic Energy Medical Center JPMC, Karachi, Pakistan.
Pakistan Health Research Council Research Centre, Karachi, Pakistan.
Pakistan Health Research Council Research Centre, Karachi, Pakistan.
Objectives: To estimate the cancer incidence by age group and gender for the population of Karachi Division by analyzing the Karachi Cancer Registry data of 2017-19. Settings: The population of Karachi division is 16.1 million according to national census 2017. ‘Karachi Cancer Registry’ which is a part of ‘National Cancer Registry’ is collecting data from eight major hospitals in Karachi since 2017. For outcome measures, cancer counts and the age standardized incidence rates (ASIR) per 100,000 population were computed for age groups (0–14, 15–19 and ≥20 years), in both genders and all cancer site/type. Methods: The population denominators were based on the population of Karachi division estimated at 16.1 million in the population census, 2017. Counts and age-standardized incidence rates (ASIR) were calculated for each of the three age categories. Results: From Jan 2017 till Dec 2019 a total of 33,309 malignant cases were recorded in KCR database comprising 17,490 (52.5%) females and 15,819 (47.5%) males. ASIRs in age groups 0-14, 15-19 and ≥ 20 years, among female were 11.5, 2.4 and 223.6 and in males were 17.6, 3.2 and 216.7 respectively. The commonest diagnosis in children, adolescent and adults were (1) among females: children; bone (3.12), leukemia (2.09) brain/CNS (1.26); in adolescents: bone (0.78), brain/CNS (0.27), connective and soft tissue (0.11), in adults: breast cancer (76.07), oral cancer (16.68) and ovary (10.89) respectively, and (2) among males: children; bone (4.56), leukemia (2.79) and brain/CNS (1.88); in adolescent; bone (1.19), brain/CNS (0.31) and leukemia (0.21) and in adults: oral cancer (42.83), liver (16.10) and bone (13.37) respectively. Conclusion: Oral Cancer, a largely preventable cancer is the leading cancer in Karachi adult males while in female adults Breast Cancer is the leading cancer followed by Oral Cancer. In children and adolescents Bone, Leukemia and Brain/CNS malignancies are most common.
https://journal.waocp.org/article_89345_8db826f434fea23e9c6445bb28bac640.pdf
Karachi
cancer
Registry
Incidence
Pakistan
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3259
3266
10.31557/APJCP.2020.21.11.3259
89346
Childhood Cancer Survival in Brunei Darussalam
Elvynna Leong
elvynna.leong@ubd.edu.bn
1
Sok King Ong
sokking.ong@moh.gov.bn
2
Fadzilah Jali
deelahfadzilah@gmail.com
3
Noraslinah Ramlee
dr.noraslinah@pjscbrunei.com
4
Faculty of Science, Universiti Brunei Darussalam, Jln Tungku Link, Brunei Darussalam.
NCD Prevention Unit, Ministry of Health, Commonwealth Drive, Brunei Darussalam.
Faculty of Science, Universiti Brunei Darussalam, Jln Tungku Link, Brunei Darussalam.
Early Detection & Cancer Prevention Services, Pantai Jerudong Specialist Centre, Brunei Darussalam.
Background: This study aims to determine the survival rates for children and adolescents aged 0-19 years diagnosed with childhood cancer and to evaluate the associated factors for childhood cancer survival in Brunei Darussalam. Methods: The analysis was based on de-identified data of 263 childhood cancer for the period 2002 to 2017 retrieved from a population-based cancer registry. Overall survival was estimated using the Kaplan-Meier method. Univariate analysis, using the log-rank test, was used to examine the differences in survival between groups. Multivariate analysis, using the Cox Proportional Hazard (PH) regression model, was used to estimate the hazard ratios (HRs) and select the significant associated factors for childhood cancer patients’ survival. Results: The overall 1-, 5- and 10-year survival rates for all childhood cancers combined were 79.4%, 70.0% and 68.8% respectively. The most common types of cancer were leukemias, malignant epithelial neoplasms, lymphomas and tumours of the central nervous system (CNS). The 5-year survival estimates were highest for malignant epithelial neoplasms (84.2%) while the lowest was tumours of the CNS (44.1%). Log rank tests showed significant differences in childhood cancer patients’ survival between tumour types and period of diagnosis. In the Cox PH analysis, the presence of lymphomas, gonodal and germ cell neoplasms, and malignant epithelial neoplasms compared to leukemia; children aged 1-4 and 5-9 years compared to adolescents aged 15-19 years; and periods of diagnosis in 2002-2006 and 2007-2011 compared to 2012-2017 were significantly associated with lower hazard of death in this study. Conclusion: This study provides a baseline measurement of childhood cancer survival for monitoring and evaluation of cancer control programmes, to allow planning of cancer control program strategies such as surveillance, screening, and treatment to improve childhood survival rates in Brunei Darussalam.
https://journal.waocp.org/article_89346_bac0078600a8e5ab3741ce1b68e071c6.pdf
hazard
Cox PH
Prognostic factor
population-based
cancer registry
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3267
3277
10.31557/APJCP.2020.21.11.3267
89347
Clinical Utility of BRAF, NRAS, and TERT Promoter Mutation in Preoperative Thyroid Fine-Needle Aspiration Biopsy: A Diagnostic Study From Dharmais Cancer Hospital
Adhitya Perdana
adhitya.bayu99@gmail.com
1
Rizky Putri
putri.brahma@gmail.com
2
Rachmawati Rachmawati
iraimaduddin@gmail.com
3
Bob Andinata
bobandinata@gmail.com
4
Bayu Brahma
bbrahma_s@yahoo.com
5
Department of Research and Development, Dharmais Cancer Hospital - National Cancer Center, Jakarta, Indonesia.
Department of Anatomical Pathology, Dharmais Cancer Hospital - National Cancer Center, Jakarta, Indonesia.
Surgical Oncology Study Program, Department of Surgery, Faculty of Medicine Universitas Indonesia - Cipto Mangunkusumo Hospital, Jakarta, Indonesia.
Department of Surgical Oncology, Dharmais Cancer Hospital - National Cancer Center, Jakarta, Indonesia.
Department of Surgical Oncology, Dharmais Cancer Hospital - National Cancer Center, Jakarta, Indonesia.
Objective: Molecular testing of thyroid nodules becomes important for improving the accuracy of fine-needle aspiration biopsy (FNAB). This study aimed to investigate the diagnostic utility of BRAF, NRAS, and TERT promoter mutation in thyroid nodules at Dharmais Cancer Hospital.Methods: We performed a prospective diagnostic study involving 50 patients with thyroid nodules who needed surgery between September 2013 and August 2014. Mutational hotspots in BRAF exon 15, NRAS exon 3, and TERT promoter region were analyzed by Sanger sequencing from FNAB specimens. Cytology and molecular data were compared to histopathology results.Result: Of the 50 cases included in the analysis, 39 cases (78%) were thyroid malignancies. Mutations of BRAF, NRAS, and TERT promoter were detected in 31% (12/39), 18% (7/39), and 13% (5/39) cases, respectively. BRAF and NRAS mutations were found mutually exclusive, while all of TERT promoter mutation was found coexistent either with BRAF (40%) or NRAS (60%). The combination of FNAB cytology and molecular testing resulted in 69% sensitivity, 100% specificity, 100% positive predictive value, 48% negative predictive value, and 76% accuracy.Conclusion: Molecular testing of BRAF, NRAS, and TERT mutations improve the sensitivity of thyroid FNAB and is beneficial for more definitive treatment in selective cases. However, the NPV is relatively low to avoid the need for diagnostic surgery. Therefore, further studies to identify more sensitive methods and more comprehensive molecular markers in the diagnosis of thyroid nodules are needed.
https://journal.waocp.org/article_89347_bed0bdb41c89b2f807aed1a812a968f1.pdf
fine-needle aspiration biopsy
hotspot mutation
Thyroid cancer
Diagnostic
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3279
3284
10.31557/APJCP.2020.21.11.3279
89348
Significance of Some Non-Invasive Biomarkers in the Early Diagnosis and Staging of Egyptian Breast Cancer Patients
Tarek Motawi
tarek.motawi@pharma.cu.edu.eg
1
Nadia Zakhary
nadia.zakhary@nci.cu.edu.eg
2
Hebatallah Darwish
hebatallah.darwish@fue.edu.eg
3
Hassan Abdullah
hassan.abdullah@nci.cu.edu.eg
4
Samer Tadros
stadros@msa.eun.eg
5
Department of Biochemistry, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
Department of Cancer Biology, National Cancer Institute, Cairo University, Cairo, Egypt.
Pharmacology, Toxicology and Biochemistry Department, Faculty of Pharmaceutical Sciences and Pharmaceutical Industries, Future University in Egypt (FUE), Cairo, Egypt.
Department of Surgical Oncology, National Cancer Institute, Cairo University, Cairo, Egypt.
Department of Biochemistry, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), October, Egypt.
Introduction: Breast cancer is one of the most relevant malignancies among women. Early diagnosis and accurate staging of breast cancer is important for the selection of an appropriate therapeutic strategy and achieving a better outcome. Aim: This study aimed to explore the significance of some non-invasive biomarkers in the early diagnosis and staging of Egyptian breast cancer patients. Subjects and Methods: A total of 135 female patients with physically and pathologically confirmed breast cancer and 40 unrelated controls as well as 40 patients with benign breast mass were enrolled in this study. The malignant breast cancer group was further divided into four groups according to tumor size. Serum levels of carcinoembryonic antigen-related cell adhesion molecule-1 (CEACAM1), resistin and visfatin were determined by enzyme immunoassay. Results: Elevated levels of CEACAM1, resistin and visfatin were observed in breast cancer patients when compared with normal control and benign groups. The cutoff values, sensitivities and specificities of these biomarkers were appropriate for the discrimination of breast cancer from controls. Additionally, the serum levels of visfatin increased positively with tumor size and consequently with breast cancer stages. Conclusion: CEACAM1, resistin and visfatin are valuable in early diagnosis of breast cancer, with visfatin being preferentially used in staging.
https://journal.waocp.org/article_89348_35bdc3045f97db206ecead18d7a364e9.pdf
breast cancer
Early Diagnosis
CEACAM1
visfatin and resistin
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3285
3290
10.31557/APJCP.2020.21.11.3285
89349
Baseline Sarcopenia is Associated with Lack of Response to Therapy, Liver Decompensation and High Mortality in Hepatocellular Carcinoma Patients
Hanaa Badran
hanabadran21@yahoo.com
1
Maha Mohammad Elsabaawy
maha.ahmed@liver.menofia.edu.eg
2
Amr Ragab
amr.ragab124@yahoo.com
3
Rasha Aly
rasha_radiology@yahoo.com
4
Ayman Alsebaey
aymanalsebaey@liver.menofia.edu.eg
5
Aliaa Sabry
aliaasabry@liver.menofia.edu.eg
6
Department of Hepatology and Gastroenterology, National Liver Institute, Menoufia University, Shebeen El-Koom, Egypt.
Department of Hepatology and Gastroenterology, National Liver Institute, Menoufia University, Shebeen El-Koom, Egypt.
Department of Hepatology and Gastroenterology, National Liver Institute, Menoufia University, Shebeen El-Koom, Egypt.
Department of Diagnostic Medical Imaging and Interventional Radiology; National Liver Institute, Menoufia University, Shebeen El-Koom, Egypt.
Department of Hepatology and Gastroenterology, National Liver Institute, Menoufia University, Shebeen El-Koom, Egypt.
Department of Hepatology and Gastroenterology, National Liver Institute, Menoufia University, Shebeen El-Koom, Egypt.
Objective: hepatocellular carcinoma (HCC) is a dreadful complication of liver cirrhosis. Aim was to study the effect of sarcopenia on the survival in patients with HCC. Methods: we included 262 patients and were followed up for 12 months. Sarcopenia was calculated by skeletal muscle index (SMI). Sarcopenia was defined by SMI ≤39 cm2/m2 for women and ≤50 cm2/m2 for men. Results: patients with sarcopenia (n= 113, 43.1%) were older, mainly males, Child-Pugh class B and smokers. Patients with sarcopenia had lower survival than those without (10.09 vs. 11.72 months). Survival was also lower in Barcelona clinic liver cancer stage C than B and A (9.02 vs. 11.21 vs. 11.89 months). Age and sarcopenia were hazardous of mortality (p <0.05). There was statistically significant difference of serial SMI in patients without baseline sarcopenia unlike patients with baseline sarcopenia. On follow up patients with sarcopenia had higher incidence of ascites (45% vs. 20.4%), spontaneous bacterial peritonitis (21.7% vs. 11.6%), hepatic encephalopathy (28% vs. 11.5%) and bleeding (22.9% vs. 12.7%). Totally patients with sarcopenia had higher incidence of progressive HCC (39% vs. 25.5%). Conclusion: Sarcopenia is associated with lack of response to therapy, liver decompensation and higher mortality in hepatocellular carcinoma patients.
https://journal.waocp.org/article_89349_cc643ce4770cf7eeca5890c5d5c453d5.pdf
skeletal muscle index
sarcopenia
survival
Hepatocellular carcinoma
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3291
3299
10.31557/APJCP.2020.21.11.3291
89350
Subtype Specific Nasopharyngeal Carcinoma Incidence and Survival Trends: Differences between Endemic and Non-Endemic Populations
Ilona Argirion
argirion@umich.edu
1
Katie Zarins
kmrents@umich.edu
2
Krittika Suwanrungruang
krisuw@kku.ac.th
3
Donsuk Pongnikorn
donsukp@hotmail.com
4
Imjai Chitapanarux
imjai@hotmail.com
5
Hutcha Sriplung
hutcha.s@psu.ac.th
6
Patravoot Vatanasapt
patvat@kku.ac.th
7
Laura Rozek
rozekl@umich.edu
8
Department of Environmental Health Sciences, University of Michigan School of Public Health, Ann Arbor, MI, Thailand.
Department of Environmental Health Sciences, University of Michigan School of Public Health, Ann Arbor, MI, Thailand.
Cancer Unit, Srinagarind Hospital, Khon Kaen University, Khon Kaen, Thailand.
Lampang Cancer Hospital, Lampang, Thailand.
Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Songkhla Cancer Registry, Prince of Songkla University, Songkhla, Thailand.
Department of Otorhinolaryngology, Faculty of Medicine, Khon Kaen University, Thailand.
Department of Environmental Health Sciences, University of Michigan School of Public Health, Ann Arbor, MI, Thailand.
Background: While nasopharyngeal carcinoma (NPC) is rare in non-endemic regions such as the North America, endemic countries, such as Thailand, continue to struggle with high incidence and mortality rates. NPC has a complex etiology that varies by histological subtype. Methods: NPC cases (1990-2014) were identified using the International Classification of Diseases for Oncology (ICD-O) code C11 from the Chiang Mai, Khon Kaen, Lampang, and Songkhla cancer registries and compared to Asian/Pacific Islanders (A/PI) from the US SEER program. Age-standardized incidence rates and changes in annual percent change (APC) for overall and subtype specific NPC were assessed using R and Joinpoint. Kaplan Meier curves were generated in SAS to evaluate differences in survival by sex, year of diagnosis and histological subtype. Five-year relative survival estimates were calculated between 2000-2014. Results: Non-keratinizing NPC predominated across all registries except Songkhla, where the keretinizing subtype made up ~60% of all reported cases. Incidence of keratinizing NPC significantly decreased among Chiang Mai males between 1996 and 2014 (APC:-13.0 [95%CI:-16.2, -9.6]), Songkhla females (APC:-4.0 [95%CI: -7.4, -0.5]) and males between 2006 and 2014 (APC:-15.5 [95%CI:-25.0, -4.7]), as well as A/PI females (APC:-5.1 [95%CI:-6,7, -3.4]) and males (APC: -4.8 [95%CI:-5.9, -3.7]). Non-keratinizing NPC increased among Songkhla males (APC:4.3 [95%CI:1.8, 6.9]). The keratinizing subtype exhibited the worst survival, while the non-keratinizing undifferentiated subtype had the best survival. Although US A/PI had the highest 5-year relative survival estimates, among the Thai registries Chiang Mai had the best and Lampang the worst survival. Conclusion: Although US A/PIs exhibited similar rates of NPC as seen in the endemic Thai population, improved tobacco control has led to a decrease in keratinizing NPC incidence irrespective of geography. Additionally, while challenges associate with access to care may still exist among rural Thais, chemoradiation was shown to confer a survival benefit in non-keratinizing NPC treatment.
https://journal.waocp.org/article_89350_9d1e5de4cc68329d04347321876dacb5.pdf
Nasopharyngeal carcinoma
incidence rates
survival
global epidemiology
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3301
3307
10.31557/APJCP.2020.21.11.3301
89351
Time from Self-Detection of Symptoms to Seeking Definitive Care among Cervical Cancer Patients
Shivaraj Somanna
shivarajns@gmail.com
1
Srinivasa Nandagudi Murthy
nsmurthymsrmc@gmail.com
2
Ramesh Chaluvarayaswamy
ramesh_kidwai@yahoo.co.in
3
Nea Malila
nea.malila@cancer.fi
4
Department of Community Medicine, Ramaiah Medical College, Bengaluru, India.
Department of Epidemiology and Biostatistics, Kidwai Memorial Institute of Oncology, Bengaluru, India.
Finnish Cancer Registry, Finland.
Finnish Cancer Registry, Finland.
Background: India had the burden of 97,000 new cases of cervical cancer with 60,000 deaths accounting nearly one-third of global cervical cancer deaths during the year 2018. Cervical cancer is the leading cause of cancer mortality in India. The present study aims to estimate the time interval between self-detection of cervical cancer symptoms and seeking care and different barriers for the possible time lag in seeking care. Methods: A cross-sectional study was undertaken from April 2017 to September 2017 in a regional cancer centre in the south of India. The centre has both a population and a hospital-based cancer registry. Cervical cancer cases (N= 210) with histological confirmation were interviewed at the hospital using a pre-tested semi-structured questionnaire. Results: The median time interval between the self-detection of cervical cancer symptoms and first contact with the general physician was 80 [IQR 45-150] days. The overall median time interval between the self-detection of symptoms to the initiation of primary treatment was 123[IQR 83-205] days. The major perceived reason for not seeking medical care was a lack of awareness in identifying cervical cancer symptoms in 183(92.9%) women. Conclusion: The median time of 80 days was observed from the self-detection of cervical cancer symptoms to the first contact with a general physician. Lack of awareness of patients pertaining to cancer symptoms was the major concern in seeking cancer care.
https://journal.waocp.org/article_89351_1784bd13a52febffa6db5d92a3f5fb1d.pdf
Cervical cancer
Time interval
Cancer symptoms
seeking cancer care
perceived reasons
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3309
3315
10.31557/APJCP.2020.21.11.3309
89352
Factors Affecting the Intention to Quit among Women Smokers in Turkey
Omer Alkan
oalkan@atauni.edu.tr
1
Aysenur Demir
aysenurdemrr@hotmail.com
2
Department of Econometrics, Faculty of Economics and Administrative Sciences, Ataturk University, Erzurum, Turkey.
Department of Econometrics, Faculty of Economics and Administrative Sciences, Ataturk University, Erzurum, Turkey.
Objectives: Tobacco use is an important public health problem that affects adversely the quality of life. A person’s attitude toward quitting tobacco use can be reflected by the desire or intention to quit smoking. The aim of this study was to determine risk factors affecting women’s intention to quit tobacco in Turkey. Methods: In this study, the data obtained from the Global Adult Tobacco Survey (GATS) were used. The GATS is a standard method used in countries to monitor and evaluate the frequency of tobacco use in adults and tobacco control practices. The data used in the study was obtained from the GATS carried out in Turkey in 2008 and 2012. The data related to 1248 women smoking tobacco were used in the analysis. The relationship between women’s intention to quit tobacco use and socio-demographic and economic variables was examined. Results: Men were excluded from the analysis because the focus of the study was women. It was determined that 732 of 1248 women using tobacco intend to quit smoking tobacco. 40.4% of women smoking tobacco are primary school graduates. Women, who were aware of anti-smoking messages and exposed to stimulants that promote smoking, were 36.4% and 27% more likely to intend to stop tobacco use after the next month, respectively. It was observed that women living in a house, where smoking is allowed, are less likely to quit smoking than others (ME = -0.522). This shows the importance of domestic restrictions. Conclusion: Intending to quit is an important preliminary step to quit. Understanding the factors associated with the intention to quit smoking can help tobacco users to stop using it and shape effective policies to increase the quit rates.
https://journal.waocp.org/article_89352_9f5ff674c49ddc9bc20cc83f7f96afbb.pdf
intention to quit
smoking cessation
multinomial probit regression
Turkey, Global Adult Tobacco Survey
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3317
3323
10.31557/APJCP.2020.21.11.3317
89353
Stathmin! An immunohistochemical analysis of the novel marker in Oral Squamous Cell Carcinoma and Oral Leukoplakia.
Purnima Vadla
purnimaswamy.vadla@gmail.com
1
Sivaranjani Yeluri
ysivaranjani1@gmail.com
2
G Deepthi
drdeepthireddy999@gmail.com
3
Venkateswara Rao Guttikonda
mamatadental@yahoo.co.in
4
Sravya Taneeru
saibds95@gmail.com
5
Srikanth Naramala
dr.srikanth83@gmail.com
6
Department of Oral Pathology and Microbiology, Mamata Dental College, Khammam, Telangana, India.
Department of Oral Pathology and Microbiology, Mamata Dental College, Khammam, Telangana, India.
Department of Oral Pathology and Microbiology, Mamata Dental College, Khammam, Telangana, India.
Department of Oral Pathology and Microbiology, Mamata Dental College, Khammam, Telangana, India.
Department of Oral Pathology and Microbiology, Mamata Dental College, Khammam, Telangana, India.
Department of Oral Pathology and Microbiology, Mamata Dental College, Khammam, Telangana, India.
Background: Stathmin is an intracellular phosphoprotein that controls the microtubule dynamics by further regulating proper attachment and alignment of chromosomes in a dividing cell. Thus, any mutation or aberrantly expressed protein that reduces the fidelity of spindle assembly will enhance chromosomal instability contributing to aneuploidy. Oral Squamous Cell Carcinoma is an extensively studied malignancy that occurs due to accumulated genetic changes due to carcinogens. The current study is done to evaluate the stathmin role and its expression in OSCC and Oral epithelial dysplasia (OED). Objective: The aim of the present study is to evaluate the role of stathmin in OSCC and Oral dysplasia and also to correlate the expression of Stathmin with respect to the different histopathological grades of OED and OSCC. Materials and Methods: 30 neutral buffered formalin fixed, paraffin embedded (FFPE) tissues of Oral Leukoplakia/OED and 30 FFPE tissues of OSCC were subjected to immunohistochemistry with stathmin antibody. Five fields of each case with 300 cells were examined and a mean percentage of positive–stained slides were determined. The percentages were recorded accordingly with their respective histological grades. The results were analysed statistically. Results: The results of the present study demonstrated higher mean values of stathmin in tissues with OSCC (2.50) compared to leukoplakia (2.11) and normal tissues (0.00) with a high level of statistical significance (0.0001). There is also an increase in the percentage levels of stathmin with increase in the histological grade of differentiation in OSCC as well as leukoplakia. Conclusion: The present study found a statistical correlation between increased grades of the disease with expression levels of stathmin. This confirms that stathmin expression can contribute to disease progression and that stathmin might have a potential role as an early diagnostic biomarker and can be a therapeutic target for OSCC.
https://journal.waocp.org/article_89353_c85c0ad65abc7107d581ff3749f88ced.pdf
stathmin
oral squamous-cell carcinoma
oral epithelial dysplasia
Biomarker
poor prognosis
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3325
3329
10.31557/APJCP.2020.21.11.3325
89354
Efficacy of Oral Vitamin A in Reducing β-hCG Levels in Low-Risk Gestational Trophoblastic Neoplasia Patients
Yudi Hidayat
yudi.mulyana@unpad.ac.id
1
Eppy Darmadi A
eppyda@yahoo.com
2
Sylvia Rachmayati
rachma210@gmail.com
3
Windy Kusumah
windypuspa@gmail.com
4
Tono Djuwantono
djuwantono@yahoo.com
5
Akhmad Pramatirta
dryogipramatirta@yahoo.com
6
Dodi Suardi
dodisuardi08@gmail.com
7
Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Padjadjaran/Dr. Hasan Sadikin General Hospital, Bandung, Indonesia.
Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Padjadjaran/Dr. Hasan Sadikin General Hospital, Bandung, Indonesia.
Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Padjadjaran/Dr. Hasan Sadikin General Hospital, Bandung, Indonesia.
Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Padjadjaran/Dr. Hasan Sadikin General Hospital, Bandung, Indonesia.
Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Padjadjaran/Dr. Hasan Sadikin General Hospital, Bandung, Indonesia.
Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Padjadjaran/Dr. Hasan Sadikin General Hospital, Bandung, Indonesia.
Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Padjadjaran/Dr. Hasan Sadikin General Hospital, Bandung, Indonesia.
Objective: Low-risk gestational trophoblastic neoplasia (GTN) is generally treated with single agent chemotherapy and methotrexate (MTX) as a first-line therapy. Vitamin A helps to increase trophoblast cell regression, as well as to decrease β-hCG levels. Vitamin A also increases the effectiveness of MTX by inducing more malignant cell death than MTX alone. Therefore, the aim of the current study was to analyze the changes in β-hCG levels in low-risk GTN patients following vitamin A administration. Methods: This study was a randomized clinical trial, which examined initial serum vitamin A and β-hCG levels in GTN patients before and after three cycles of MTX therapy. Patients were given vitamin A supplementation of 6,000 IU (1.8 mg RAEs) per day, and the changes in serum β-hCG were observed after three cycles. Patients were grouped by β-hCG levels (decreased or stagnant). Results: A total of 32 low-risks GTN patients were divided into the intervention group (16 patients who received vitamin A supplementation) and the control group (16 patients who did not receive vitamin A supplementation). In the intervention group, the average initial β-hCG level was 170,949.3 ± 354,452.1 mIU/mL, and the average β-hCG post-cycle level was 1,611.9 ± 3,652.5 mIU/mL. In the control group, the average initial β-hCG level was 178,834.1 ± 2913844.6 mIU/mL, and the average β-hCG post-cycle level was 25,388.5 ± 58,437.7 mIU/mL. Conclusion: In patients with low-risk GTN who underwent MTX chemotherapy, the levels of β-hCG and the incidence of chemo resistance in the intervention group were lower than those in the control group. Older age may also influence the incidence of chemo resistance in GTN patients. Oral administration of 6,000 IU vitamin A could help to reduce β-hCG levels in low-risk GTN patients who receive MTX chemotherapy.
https://journal.waocp.org/article_89354_556e90019540ec2fb59b20c06bb33283.pdf
Keywords: β-hCG levels
Gestational trophoblastic neoplasia
methotrexate chemotherapy
vitamin A
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3331
3338
10.31557/APJCP.2020.21.11.3331
89355
A Study of Human Papillomavirus Genotypes Specificity and the Vaccines Introducing System for Korean-Chinese Women in the Yanbian Autonomous Region
Qunying Wu
wqy1977@hotmail.com
1
Jin-Ho Kuk
kjino77@naver.com
2
Young-Joon Ryu
mindmader@gmail.com
3
Department of Pathology, College of Medicine, Kangwon National University, Chun-Cheon, Republic of Korea.
Department of Pathology, College of Medicine, Kangwon National University, Chun-Cheon, Republic of Korea.
Department of Pathology, College of Medicine, Kangwon National University, Chun-Cheon, Republic of Korea.
Objectives: Human papillomavirus (HPV) is a major cause of cervical cancer in women. The characteristics of HPV infection vary; therefore, it is necessary to identify the most common HPV genotypes among a group of subjects when introducing a vaccine program. Currently, in the Yanbian Autonomous Region, no HPV vaccinations are not provided, and no data has been reported regarding HPV rates or genotype prevalence. We aimed to find the most suitable HPV vaccine for this region and reasons why no vaccine has been introduced. Methods: HPV genotyping of 200 Korean-Chinese women living in the Yanbian Autonomous Region who visited the hospital for annual health examination was done. We also checked main factors necessary for HPV vaccine administrative system; (1) vaccine manufacturers in China, (2) vaccine importers, (3) vaccine suppliers, (4) applicable vaccine laws, (5) the HPV vaccine permit system in Jilin Province, and (6) vaccination hospital facilities-were assessed by direct inquiry and search. Results: The results showed that HPV genotypes 52, 58, 16, 53, and 33 were the most common among Korean-Chinese women. These results differed from those previously reported for Korean or Chinese women. All elements necessary for introduction of HPV vaccine were prepared, but there is no HPV vaccination plan based on epidemiological investigation. Conclusions: Gardasil® 9 should be the most suitable vaccine for Korean-Chinese women with HPV infection and cervical cancer in this region considering the prevalence of certain genotypes. Governments and medical institutions should take an active stance on HPV vaccination to lower the incidence of cervical cancer here. Our study may serve as an important reference for introducing a Chinese government program designed to prevent cervical cancer.
https://journal.waocp.org/article_89355_dac9d881378e2a1f48e376232d8faf55.pdf
human papillomavirus
Genotype
Vaccine
Law
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3339
3343
10.31557/APJCP.2020.21.11.3339
89356
Tissue Expression Of LPHN3 in Breast Cancer: An Immunohistochemistry Method
Kwuntida Kotepui
kwuntida.ut@wu.ac.th
1
Manas Kotepui
manas.ko@wu.ac.th
2
Duangjai Piwkham
duangjai.ph@wu.ac.th
3
Apiram Songsri
papiram@gmail.com
4
Lek Charoenkijkajorn
lekpath@gmail.com
5
Tidamas Kongnok
mimi_belt@hotmail.com
6
Yupaporn Chanil
yupaporn3556@gmail.com
7
Medical Technology Program, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80161, Thailand.
Medical Technology Program, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80161, Thailand.
Medical Technology Program, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80161, Thailand.
Department of Pathology, Hatyai Hospital, Songkhla 90110, Thailand.
Department of Pathology, Hatyai Hospital, Songkhla 90110, Thailand.
Medical Technology Program, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80161, Thailand.
Medical Technology Program, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80161, Thailand.
Objective: Breast cancer is one of the most important public health problems among women worldwide. It is a major cause of morbidity especially among women in developing countries including Thailand. The purpose of this study was to study the expression of LPHN3 protein in normal breast tissue compared to breast cancer tissue. Methods: We had studied the expression of LPHN3 in 65 breast tissues using an immunohistochemistry method. The association between LPHN3 expression and breast cancer metastasis to nearby axillary lymph nodes was also examined. Results: Among the 65 breast cancer and normal breast tissues examined, LPHN3 expression with an immunohistochemistry index (IHC index) greater than 4 was more frequently found in breast cancer tissues than in normal breast tissues (P-value = 0.001, OR (95% CI) = 7.04 (2.16-23)). Moreover, a high expression of LPHN3 (IHC index > 4) was more frequently found in breast cancer tissues with negative axillary lymph nodes than in those with positive ones (P-value = 0.038, OR (95% CI) = 0.25 (0.07-0.96)). LPHN3 protein might be a new metastasis suppressor gene in breast cancer and a marker for breast cancer metastasis prevention. Conclusions: The present study indicated that a decrease of LPHN3 protein expression in breast cancer tissue might be a marker indicating the aggressiveness of breast cancer. These results also suggested that a decrease of LPHN3 expression could be functionally involved in breast cancer progression and metastasis.
https://journal.waocp.org/article_89356_c11bca4c4fa112ffe00f0d581e7f50df.pdf
Keywords: LPHN3
breast cancer
Lymph node metastasis
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3345
3348
10.31557/APJCP.2020.21.11.3345
89357
Role of Napsin A and Survivin Immunohistochemical Expression in Bronchogenic Adenocarcinoma
Maha Mohammed Salama
maha.elsayed@kasralainy.edu.eg
1
Department of Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt.
Background: Lung cancer, being the leading cause of cancer deaths with most patients diagnosed at a late stage, represents a major burden in developing countries especially with both air pollution and tobacco use increasing. With the evolution of new, successful therapies that target lung adenocarcinoma, it became of utmost importance to diagnose lung adenocarcinoma. Despite considering TTF-1 as the predominant marker for identifying lung adenocarcinoma but it has limited sensitivity and specificity, which means that its expression decreases in relation to the degree of tumor differentiation. Aim of work: this study intended to evaluate the use of Napsin A in lung adenocarcinoma, and observe if it can withstand along the different lines of tumor differentiation and Survivin as a marker of poor prognosis. Materials and Methods: Forty paraffin blocks of bronchogenic carcinoma were collected and studied immunohistochemically against Napsin A and Survivin. Results: There was a statistically significant relation between Napsin A reactivity and tumor grade as 72% of grade II as well as all cases of grade I were strongly positive compared to none of grade III cases. Another statistically significant relation between Survivin reactivity and tumor grade was observed as all grades I and II cases showed labeling index <10%, while all grade III cases showed labeling index >10%. Conclusion: Napsin A is a good prognostic marker while Survivin stands as a poor one for lung adenocarcinoma with a statistically inverse relation between the two, which means that Napsin A can’t be used as a marker for diagnosing poorly differentiated tumors.
https://journal.waocp.org/article_89357_3542b947232fa53ae2d60251ca870606.pdf
Nap A
Lung
cancer
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3349
3355
10.31557/APJCP.2020.21.11.3349
89358
Human Papillomavirus 16 and 18 Infection in Oral Cancer in Thailand: A Multicenter Study
Nicha Komolmalai
nicha_1990@hotmail.com
1
Surawut Pongsiriwet
2
Nirush Lertprasertsuke
3
Suree Lekwanavijit
4
Sompid Kintarak
5
Ekarat Phattarataratip
6
Ajiravudh Subarnbhesaj
7
Kittipong Dhanuthai
8
Risa Chaisuparat
9
Anak Iamaroon
iamaroon@yahoo.com
10
Department of Oral Biology and Diagnostic Sciences, Faculty of Dentistry, Chiang Mai University, Chiang Mai, Thailand.
Department of Oral Biology and Diagnostic Sciences, Faculty of Dentistry, Chiang Mai University, Chiang Mai, Thailand.
Department of Pathology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Department of Pathology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Department of Stomatology, Faculty of Dentistry, Prince of Songkla University, Songkhla, Thailand.
Department of Oral Pathology, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.
Department of Oral Diagnosis, Faculty of Dentistry, Khon Kaen University, Khon Kaen, Thailand.
Department of Oral Pathology, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.
Department of Oral Pathology, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.
Department of Oral Biology and Diagnostic Sciences, Faculty of Dentistry, Chiang Mai University, Chiang Mai, Thailand.
Objectives: To identify the prevalence of high-risk human papillomavirus (HPV) genotypes 16 and 18 among patients with oral squamous cell carcinoma (OSCC) in Thailand and investigate the associations of p16 expression and HPV16/18 with the demographic, clinicopathologic, and risk parameters. Materials and Methods: A total of 403 formalin-fixed paraffin-embedded OSCC specimens from four centers in four regions were obtained. p16 expression was evaluated by immunohistochemistry. The detection of HPV16/18 DNA was performed by polymerase chain reaction. Results: Of all, 172 specimens (42.7%) were presented with amplifiable extracted DNA. Among these, 62.8% were positive for p16, 8.1% were positive for HPV16/18, and 5.8% were positive for both methods. Of all HPV-positive specimens, HPV18 was detected in 57.1%; HPV16 in 14.3%; and HPV16 and 18 (co-infection) in 28.6%. The prevalence of HPV16/18 varied between centers, with the highest rate in the northern center (20.0%). There was no significant correlation between p16 expression and HPV16/18. There were no significant associations of p16 expression and/or HPV16/18 with all variables. Conclusions: The prevalence of HPV16/18 infection in OSCC geographically varied in Thailand, with the highest rate in the northern region. Poor correlation between p16 and HPV16/18 suggests p16 not be used as a surrogate marker for HPV-positive OSCC.
https://journal.waocp.org/article_89358_1098cb20dad12e4b0278ce13479ff4c6.pdf
HPV16/18
multicenter study
oral squamous cell carcinoma
P16
Thailand
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3357
3363
10.31557/APJCP.2020.21.11.3357
89359
DNA Methyltransferases as Potential Biomarkers for HCV Related Hepatocellular Carcinoma
Mona Hassouna
ysahassouna@yahoo.com
1
Mary Naguib
dr_maryalbert@yahoo.com
2
Enas Radwan
iradwanm@hotmail.com
3
Mohamed Abdel-Samiee
drmohammed100@yahoo.com
4
Suzanne Estaphan
sestaphan@kasralainy.edu.eg
5
Eman Abdelsameea
eabdelsameea@liver-eg.org
6
Department of Clinical and Chemical Pathology, National Liver Institute, Menoufia University, Shebeen El-Kom, Egypt.
Department of Clinical and Chemical Pathology, National Liver Institute, Menoufia University, Shebeen El-Kom, Egypt.
Department of Clinical pathology, National Cancer Institute, Cairo University, Cairo, Egypt.
Hepatology and Gastroenterology, National Liver Institute, Clinical Pathology, Menoufia University, Shebeen El-Kom, Egypt.
Department of Physiology, Faculty of Medicine, Cairo University, Cairo, Egypt.
Hepatology and Gastroenterology, National Liver Institute, Clinical Pathology, Menoufia University, Shebeen El-Kom, Egypt.
Background: Hepatocellular carcinoma (HCC) is a major health concern in many countries including Egypt. The alteration in DNA methylation that was observed in HCC patients suggests a possible role of DNA methyltransferases (DNMTs) in the disease pathogenesis in addition to potential role as a disease biomarker. Aim: To study the change in DNMTs expression in chronic HCV infected patients as potential non- invasive biomarker for diagnosis of hepatocellular carcinoma. Methods: 26 patients with HCC, 45 patients with liver cirrhosis, 20 chronic HCV patients and 20 apparently healthy individuals as a control group were enrolled in this study. Real-Time Quantitative Reverse Transcription PCR (qRT-PCR) was performed for all study participants. Results: A significant difference in DNMTs expression was observed among the studied groups. Receiver operating characteristics (ROC) curve analysis revealed that with a cutoff value of 3.16 for DNMT 3A expression, sensitivity and specificity were 80.8 and 95.6% respectively and area under curve (AUC) was 0.958, p < 0.001 for discriminating hepatocellular carcinoma among post hepatitis C cirrhotic patients. Besides DNMT 3B relative expression cutoff value of 3.10 showed 84.6% sensitivity and 77.8% specificity and AUC was 0.888, p < 0.001. On the other hand, cutoff value 0.65 for DNMT1 relative expression showed 92.3% sensitivity and 44.4% specificity and AUC was 0.72, p= 0.002. DNMT1, DNMT 3A and DNMT 3B have significant positive correlation with the level of AFP (p-value = 0.003, 0.004 and 0.008 respectively). The relative expression of DNMT3B was significantly correlated to focal lesion size (p-value = 0.015). High DNMTs expression was significantly associated with the presence of multiple focal lesions but not with the Child Pugh grade (p> 0.05). Conclusion: The mRNA levels of DNMTs could be a potential biomarker for early detection of HCC development.
https://journal.waocp.org/article_89359_172548a58fabece0df75bd04edd8bf4e.pdf
Hepatocellular carcinoma
DNA methlyltransferase
Epigenetics
DNA Methylation
HCV
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3365
3371
10.31557/APJCP.2020.21.11.3365
89360
TP53, SPOP and PIK3CA Genes Status in Prostate Cancer
Mazhar Al Zoubi
mszoubi@yu.edu.jo
1
Raed Otoum
otoomraed@yahoo.com
2
Mohammed Alorjani
msalorjani@just.edu.jo
3
Samir Al Bashir
samer94@hotmail.com
4
Bahaa Al Trad
bahaa.tr@yu.edu.jo
5
Manal Abualrja
manalessam12@yahoo.com
6
Sohaib Al-Khatib
smkhatib4@just.edu.jo
7
Khalid Al-Batayneh
albatynehk@yu.edu.jo
8
Department of Basic Medical Sciences, Faculty of Medicine, Yarmouk University, Irbid 211-63, Jordan.
Department of Biological Sciences, Faculty of Science, Yarmouk University, Irbid 211-63, Jordan.
Departments of Pathology and Microbiology, Faculty of Medicine, Jordan University of Science and Technology, Irbid, Jordan.
Departments of Pathology and Microbiology, Faculty of Medicine, Jordan University of Science and Technology, Irbid, Jordan.
Department of Biological Sciences, Faculty of Science, Yarmouk University, Irbid 211-63, Jordan.
Department of Basic Medical Sciences, Faculty of Medicine, Yarmouk University, Irbid 211-63, Jordan.
Departments of Pathology and Microbiology, Faculty of Medicine, Jordan University of Science and Technology, Irbid, Jordan.
Department of Biological Sciences, Faculty of Science, Yarmouk University, Irbid 211-63, Jordan.
Recent advances in molecular biology make the identification of prostate cancer (PC) subsets a priority for more understanding of the molecular pathogenesis and treatment options. Genetic alterations in many genes such as TP53, SPOP and PIK3CA genes have been reported in PC with variable frequencies worldwide. We aimed to investigate genetic alterations in the hotspot lesions of TP53, SPOP and PIK3CA genes by direct sequencing and the expression of TP53 and PIK3CA by RT-PCR in prostate cancer, and to explore the correlation between TP53, SPOP and PIK3CA alterations and tumorigenesis of prostate cancer. Seventy-nine FFPE prostate samples from patients who underwent radical prostatectomy were obtained, subjected to genomic DNA extraction and sequenced for mutations in exons 5, 6, 7 and 8 of TP53 gene, exons 4 and 5 of SPOP gene and exons 9 and 20 of PIK3CA gene. RT-PCR was performed for the expression evaluation of the PIK3CA gene. Our results showed a high frequency of TP53 mutations (11/79, 13.9 %) in the selected population. On the other hand, SPOP and PIK3CA genes did not show any genetic alteration in the sequenced exons. PIK3CA gene overexpression was detected in 6% of the cohort by RT-PCR. TP53 mutation is the most frequent genetic alteration and likely has a major role in the pathogenesis of PC in the Jordanian population.
https://journal.waocp.org/article_89360_563e993362e0a851f14338ca186b7f5b.pdf
Tp53
SPOP
PIK3CA
Prostate Cancer
p53
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3373
3379
10.31557/APJCP.2020.21.11.3373
89361
Assessment of Tumor Angiogenesis by Expression of CD 105 in Ameloblastoma, Odontogenic Keratocyst and Central Giant Cell Lesion
Khurshid Ali
alalazizfacp@gmail.com
1
Sultan Zeb Khan
zebsultan681@gmail.com
2
Nuzhat Sultana
osaqutub@gmail.com
3
Osama Alghamdi
ozzdey84@gmail.com
4
Samrina Muhammad
albinmahfooz@gmail.com
5
Sameer Mokeem
sammokeem@gmail.com
6
Saqib Ali
mesharilk1@gmail.com
7
Tariq Abduljabbar
academicksa1@gmail.com
8
Fahim Vohra
fvohra@ksu.edu.sa
9
Department of Oral Pathology, Khyber College of Dentistry, Peshawar, Pakistan.
Department of Oral Pathology, Khyber College of Dentistry, Peshawar, Pakistan.
Department of Pathology, Northwest School of Medicine, Peshawar, Pakistan.
Department of Oral and Maxillofacial Surgery, College of Dentistry King Saud University. Riyadh, Saudi Arabia.
Department of Oral Pathology, Khyber College of Dentistry, Peshawar, Pakistan.
Department of Periodontics and Community Dentistry, College of Dentistry, King Saud University. Riyadh, Saudi Arabia.
Department of Biomedical Dental Sciences, College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.
Department of Prosthetic Dental Science, College of Dentistry, King Saud University, Research Chair for Biological Research in Dental Health, Riyadh, Saudi Arabia.
Department of Prosthetic Dental Science, College of Dentistry, King Saud University, Research Chair for Biological Research in Dental Health, Riyadh, Saudi Arabia.
Background: Angiogenesis is critical for tumor growth and reflects the aggressive behavior of invasive odontogenic lesions [like Ameloblastoma (AM) , Odontogenic Keratocyst (OKC) and Central giant cell lesion (CGCL)]. Mean vascular density (MVD) shows the angiogenic potential and CD105 is an ideal endothelial biomarker due to its specificity to new blood vessels for MVD detection. The aim of the study was to compare the MVD (angiogenic potential) among AM, OKC and CGCL in comparison to Pyogenic Granuloma (PG) using CD105 biomarker. Methods: Sixty-four primary cases of odontogenic invasive tumors (AM, OKC and CGCL) and PG, diagnosed clinically and histologically were included in the study, with 16 samples in each group. Tissue samples of peripheral AM, Peripheral GCL of jaws, malignant AM, and specimen with insufficient tissue were excluded. Tissue sections were embedded, processed and stained using Hematoxylin and Eosin (H and E). Immunohistochemistry was performed using antibodies against CD105, with positive brown cytoplasmic staining in the endothelial cells of neo-vasculature. Distinct countable, positively stained endothelial cell or clusters were evaluated under light microscope for identification of MVD. ANOVA and t-test were applied for statistical analysis of data. Results: Highest MVD was displayed in CGCL (32.99±0.77) and the minimum was observed in OKC (7.21± 0.75) respectively. CGCL showed significantly higher MVD to AM, OKC and PG lesions (p <0.05). AM (8.07± 0.36) and Odontogenic Keratocyst (7.21± 0.75) showed comparable MVD, which was lower than PG (14.7± 0.96) and CGCL vascular density (p < 0.01) respectively. Conclusion: CGCL was most aggressive, with highest MVD among the investigated odontogenic lesions (OKC, AM and PG). The proliferative aggressive behavior of Odontogenic Keratocyst is comparable to AM due to comparable mean vascular density.
https://journal.waocp.org/article_89361_fa20e682cfd441c0389a257def37597d.pdf
Blood vessels
Endoglin
Giant cell granuloma
Pathologic angiogenesis, Odontogenic tumors
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3381
3386
10.31557/APJCP.2020.21.11.3381
89362
Viability and Antioxidant Effects of Traditional Cooling Rice Powder (bedak sejuk) Made from Oryza sativa ssp. Indica and Oryza sativa ssp. japonica on UVB-Induced B164A5 Melanoma Cells
Ahmad Rohi Ghazali
rohi@ukm.edu.my
1
Raveena Vaidheswary Muralitharan
raveena.vaidheswary@gmail.com
2
Chan Kam Soon
kamsoon_chan@yahoo.com
3
Tharsini Salyam
p101575@siswa.ukm.edu.my
4
Nurul Najihah Ahmad Maulana
p101599@siswa.ukm.edu.my
5
Ummul Aqeela Balqees Mohamed Thaha
p101588@siswa.ukm.edu.my
6
Rasyidah Mohamad Halim
p101574@siswa.ukm.edu.my
7
Sajidah Suhaifi
p101617@siswa.ukm.edu.my
8
Muhamad Haziq Md Khalid
p101618@siswa.ukm.edu.my
9
Adibah Hanis Ahmad
p101614@siswa.ukm.edu.my
10
Noorhisham Tan Kofli
ntk@ukm.edu.my
11
Programme of Biomedical Science, Centre of Applied and Health Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.
Programme of Biomedical Science, Centre of Applied and Health Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.
Programme of Biomedical Science, Centre of Applied and Health Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.
Programme of Biomedical Science, Centre of Applied and Health Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.
Programme of Biomedical Science, Centre of Applied and Health Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.
Programme of Biomedical Science, Centre of Applied and Health Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.
Programme of Biomedical Science, Centre of Applied and Health Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.
Programme of Biomedical Science, Centre of Applied and Health Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.
Programme of Biomedical Science, Centre of Applied and Health Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.
Programme of Biomedical Science, Centre of Applied and Health Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.
Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, Bangi, Selangor, Malaysia.
Background: Traditional cooling rice powder (bedak sejuk) is a fermented rice-based cosmetic that is applied topically on one’s skin, as an overnight facial mask. According to user testimonies, bedak sejuk beautifies and whitens skin, whereby these benefits could be utilised as a potential melanoma chemopreventive agent. Objective: Hence, this study aimed to determine the effects of bedak sejuk made from Oryza sativa ssp. indica (Indica) and Oryza sativa ssp. japonica (Japonica) on UVB-induced B164A5 melanoma cells, and also identify the antioxidant capacities of both types of bedak sejuk. Methods: The optimum dose of Indica and Japonica bedak sejuk to treat the cells was determined via the MTT assay. Then, the antioxidant capacities of both types of bedak sejuk were determined using the FRAP assay. Results: From the MTT assay, it was found that Indica and Japonica bedak sejuk showed no cytotoxic effects towards the cells. Hence, no IC50 can be obtained and two of the higher doses, 50 and 100 g/L were chosen for treatment. In the FRAP assay, Indica bedak sejuk at 50 and 100 g/L showed FRAP values of 0.003 ± 0.001 μg AA (ascorbic acid)/g of bedak sejuk and 0.004 ± 0.0003 μg AA/g of bedak sejuk. Whereas Japonica bedak sejuk at 50 g/L had the same FRAP value as Indica bedak sejuk at 100 g/L. As for Japonica bedak sejuk at 100 g/L, it showed the highest antioxidant capacity with the FRAP value of 0.01 ± 0.0007 μg AA/g of bedak sejuk which was statistically significant (p < 0.05) when compared to other tested concentrations. Conclusion: In conclusion, Japonica bedak sejuk has a higher antioxidant capacity compared to Indica bedak sejuk despite both being not cytotoxic towards the cells. Regardless, further investigations need to be done before bedak sejuk could be developed as potential melanoma chemoprevention agents.
https://journal.waocp.org/article_89362_8de5334ca3a3746cadeaa64535fca529.pdf
Bedak sejuk
fermented rice
fermented cosmetics
Oryza sativa Indica
Oryza sativa Japonica
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3387
3392
10.31557/APJCP.2020.21.11.3387
89363
Karnofsky Performance Scale and Neurological Assessment of Neuro-Oncology Scale as Early Predictor in Glioma
Pricilla Yani Gunawan
pricilla.gunawan@uph.edu
1
Andi Islam
andiasadul@yahoo.com
2
Julius July
juliusjuly@yahoo.com
3
Ilhamjaya Patellongi
ilham_pt@yahoo.com
4
Muhammad Nasrum
nasrumm2000@yahoo.com
5
Tiara Aninditha
anindith@gmail.com
6
Department of Neurology, Faculty of Medicine, Universitas Pelita Harapan, Jl. M.H.Thamrin Boulevard 1100, Lippo Village, Tangerang 15811, Indonesia.
Department of Neurosurgery, Faculty of Medicine, Universitas Hasanuddin, Jl.Perintis Kemerdekaan KM 10, Makassar, Indonesia.
Department of Neurosurgery, Faculty of Medicine, Universitas Pelita Harapan, Jl. M.H.Thamrin Boulevard 1100, Lippo Village, Tangerang 15811, Indonesia.
Department of Physiology, Faculty of Medicine, Universitas Hasanuddin, Jl.Perintis Kemerdekaan KM 10, Makassar, Indonesia.
Molecular Biology and Immunology Laboratory, Faculty of Medicine, Universitas Hasanuddin, Jl.Perintis Kemerdekaan KM 10, Makassar, Indonesia.
Department of Neurology, Faculty of Medicine, Universitas Indonesia. Jl. Diponegoro No.71, Jakarta, Indonesia.
Objective: Glioma is one of the most frequent and disabling primary brain tumour. Patients are not only dealing with survival, but also quality of life, which remains another major concern. Karnofsky Performance Scale (KPS) is one of the most commonly used scale to assess patients’ quality of life. A recent scale, known as Neurological Assessment of Neuro-Oncology Scale, has surfaced to examine neurological disability caused by brain tumour. Previous study showed this scale to be superior to KPS in predicting survival. However, these scales have never been used to foresee functional scale improvement during disease progression. We sought to determine whether initial KPS and NANO Scale can predict functional scale improvement 2 months after surgery. Methods: Patients with glioma grade II-IV were included in the study. IDH mutation and MGMT methylation were tested. KPS and NANO scale were examined before surgery and 2 months after surgery. Favorable outcome (FO) was defined as improvement in functional scale 2 months after surgery. Patients initial functional scales were analyzed towards favorable outcome. Results: Glioma WHO grade II, III and IV was found in 17 patients (36.2%), 3 patients (6.4%) and 27 patients (57.4%) respectively. Median KPS before and 2 months after surgery were 50 (30-80) and 60 (0-100), whereas median NANO scale before and 2 months after surgery were 5 (0-12) and 3 (0-12). Favorable outcome was found in 63.8% (KPS) and 78.7% (NANO Scale). Patients initial functional scales were significantly related to FO. Conclusion: Good initial functional scales are 4 to 5 times likely of having a favorable outcome 2 months after surgery.
https://journal.waocp.org/article_89363_53edb4016ab79709aaf1251e47cd2614.pdf
Functional Scale
KPS
NANO Scale
Glioma
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3393
3403
10.31557/APJCP.2020.21.11.3393
89364
TGF-β Enhances the Anti-inflammatory Effect of Tumor- Infiltrating CD33+11b+HLA-DR Myeloid-Derived Suppressor Cells in Gastric Cancer: A Possible Relation to MicroRNA-494
Mai Moaaz
mai_moaaz@yahoo.com
1
Hassan Lotfy
hassanlotfy@hotmail.com
2
Bassem Elsherbini
bassem.elsherbini@gmail.com
3
Mohamed Motawea
dr_atef_motawea@yahoo.com
4
Geylan Fadali
mohamedabdelrahman59@yahoo.com
5
Department of Immunology and Allergy, Medical Research Institute, Alexandria University, Alexandria, Egypt.
Department of Surgery, Vascular Surgery Unit, Faculty of Medicine, Alexandria University, Alexandria, Egypt.
Department of Immunology and Allergy, Medical Research Institute, Alexandria University, Alexandria, Egypt.
Department of Experimental Surgery, Medical Research Institute, Alexandria University, Alexandria, Egypt.
Department of Pathology, Medical Research Institute, Alexandria University, Alexandria, Egypt.
Background: Accumulation of myeloid-derived suppressor cells (MDSCs) constitutes a key mechanism of tumor immune evasion in gastric cancer (GC). Therefore, searching for more accurate prognostic factors affecting their immunosuppressive role has become a growing interest in cancer immunotherapy research. Increased expression of microRNA-494 was noticed in MDSCs from tumor-bearing mice, suggesting another new therapeutic objective for cancer treatment. It was also discovered that tumor-derived transforming growth factor beta (TGF-β) is responsible for the up-regulation of microRNA-494 in MDSCs. The purpose of this study was to address the effect of recombinant (rTGF-β) on the anti-inflammatory activity of MDSCs in GC and its possible association with micro-RNA-494 expression in tumor tissue. Methods: Freshly obtained GC tumor tissue samples and peripheral blood were used for isolation of CD33+11b+HLADR- MDSCs cells from 40 GC patients and 31 corresponding controls using flow cytometry. MDSCs were co-cultured with isolated autologous T cells to assess proliferation and cytokine production in the presence and absence of rTGF-β. Real-time PCR and Enzyme linked immunosorbent assay were used to evaluate tumor expression of miRNA-494 and TGF-β respectively. Results: Results showed that rTGF-β markedly increased the suppressive ability of tumor MDSCs on proliferation of autologous T cells and interferon gamma production. However, no inhibitory effect was observed for MDSCs from circulation. In addition, infiltration of MDSCs in tumors is associated with the prognosis of GC. MiRNA-494 was also extensively expressed in tumor samples with a significant correlation to MDSCs. Conclusion: These results indicate that tumor-derived MDSCs but not circulatory MDSCs have an immunosuppressive effect on T cells, potentially involving TGF-β mediated stimulation. Results also suggest a role for miRNA-494 in GC progression. Therefore, control of TGF-β and miRNA-494 may be used as a treatment strategy to downregulate the immunosuppressive effect of MDSCs.
https://journal.waocp.org/article_89364_6b3faf455bcb7592d9014452154f5c38.pdf
myeloid-derived suppressor cells
Gastric cancer
transforming growth factor beta
microRNA-494
T cell suppression
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3405
3411
10.31557/APJCP.2020.21.11.3405
89365
Preoperative Sedentary Time Predicts Postoperative Complications in Gastrointestinal Cancer
Takuya Yanagisawa
yanagisawa1204@gmail.com
1
Hideshi Sugiura
hsugiura@met.nagoya-u.ac.jp
2
Noriatsu Tatematsu
tatematsu@met.nagoya-u.ac.jp
3
Mioko Horiuchi
h.mok1025@gmail.com
4
Saki Migitaka
uwu_ryu_mts@yahoo.co.jp
5
Keita Itatsu
kitasu@med.nagoya-u.ac.jp
6
Department of Rehabilitation, Kamiiida Daiichi General Hospital, 2-70 Kamiiida-kitamachi, Kita-ku, Nagoya, Aichi 462-0802, Japan.
Department of Integrated Health Sciences, Nagoya University Graduate School of Medicine, 1-1-20 Daiko-minami, Higashi-ku, Nagoya, Aichi 461-8673, Japan.
Department of Integrated Health Sciences, Nagoya University Graduate School of Medicine, 1-1-20 Daiko-minami, Higashi-ku, Nagoya, Aichi 461-8673, Japan.
Department of Rehabilitation, Kamiiida Daiichi General Hospital, 2-70 Kamiiida-kitamachi, Kita-ku, Nagoya, Aichi 462-0802, Japan.
Department of Rehabilitation, Kamiiida Daiichi General Hospital, 2-70 Kamiiida-kitamachi, Kita-ku, Nagoya, Aichi 462-0802, Japan.
Department of surgery, Kamiiida Daiichi General Hospital, 2-70 Kamiiida-kitamachi, Kita-ku, Nagoya, Aichi 462-0802, Japan.
Background: Gastrointestinal cancer has a high global prevalence. Postoperative complications (PCs) affect the length of hospital stay and long-term outcomes. However, it is unclear whether preoperative sedentary time is associated with PCs, independently of physical activity (PA). We aimed to investigate the association between preoperative sedentary time and PCs independently of PA in patients who underwent surgery for gastrointestinal cancer. Methods: In this prospective study, we included 112 patients who underwent colorectal cancer or gastric cancer surgery. Patient characteristics and surgery-related variables were collected. The Japanese version of the International Physical Activity Questionnaire (the usual 7-day short version) was used to assess preoperative PA and sedentary time. Patients were classified into two groups according to the grade of PCs: Clavien-Dindo (CD) grade
https://journal.waocp.org/article_89365_fbf5b6c1fab3fc0336c95aec8a815725.pdf
sedentary lifestyle
physical activity
Postoperative complications
Gastrointestinal cancer
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3413
3419
10.31557/APJCP.2020.21.11.3413
89366
The Effects of Text Message and Infographic on Reducing the Number Cigarettes Consumption: A Randomized Controlled Trial
Nirun Intarut
nirun.i@msu.ac.th
1
Ranee Wongkongdech
nokranee@gmail.com
2
Chollada Thronsao
cholada.t@msu.ac.th
3
Health Systems Science Unit, Faculty of Medicine, Mahasarakham University, Muang, Maha Sarakham, Thailand.
Health Systems Science Unit, Faculty of Medicine, Mahasarakham University, Muang, Maha Sarakham, Thailand.
Emergency Medical Operation, Faculty of Medicine, Mahasarakham University, Muang, Maha Sarakham, Thailand.
Objective: To test the effect of a text-message and infographic to promote smokers quit smoking. Methods: A randomized control trial was conducted in two provinces of northeast Thailand. Three hundred and ninety-six participants were allocated to either a text-message and infographic group or a control group. We assessed the primary outcome by self-reported 7-day point prevalence smoking abstinence. Multiple logistic regression was used to test the effect of quitting smoking. Results: At 3-month follow-up, lost to follow-up 16 participants, 380 participants were included for analysis. The difference in the rate of quitting smoking between the intervention and control groups was not found a statistical significance (17.8% versus 11.6%). However, we found a statistically significant difference in the number of cigarettes smokes (the difference: -1.74; 95%CI: -2.63, -0.84).Conclusion: No effect of text message and infographic for help smokers to quit smoking. However, the intervention showed a decrease in the number of cigarettes smoked.
https://journal.waocp.org/article_89366_a6bbba86d730f8bee28727e75fc93a2c.pdf
smoking cessation
text message
randomized controlled trial
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3421
3428
10.31557/APJCP.2020.21.11.3421
89367
Incidence of Colorectal Cancer in Selected Countries of Latin America: Age-Period-Cohort Effect
Thayana de Carvalho
thayanacalixto@gmail.com
1
Anne Karin Borges
annekarinmm@gmail.com
2
Ilce da Silva
ilceferreira@yahoo.com.br
3
National Public Health School, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
National Cancer Institute José Alencar Gomes da Silva, Rio de Janeiro, Brazil.
Department of Epidemiology and Quantitative Methods, National Public Health School, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
Objective: To estimate Age-Period-Cohort effects on colorectal, colon and rectal cancer incidence rates in Latin American countries covered by high quality Population-Based Cancer Registries. Methods: A trend study was performed using data from Cancer Incidence in Five Continents. Age-Period-Cohort effects were estimated by Poisson regression for individuals aged between 20 and 79 years with colorectal, colon and rectal cancers informed by Population-Based Cancer Registries from 1983 to 2012 in Cali (Colombia); from 1983 to 2007 in Costa Rica; and from 1988 to 2012 for both Goiânia (Brazil) and Quito (Ecuador). Goodness of fit model was tested using the deviance of the models. Results: Age effect was statistically significant for both sexes in all Population-Based Cancer Registries areas and the curves slope reached peaks in the older age groups. There were cohort effects on the incidence rates for colorectal, colon and rectal cancers in all Population-Based Cancer Registries areas, except for women in Quito. Regarding the period effect, an increased ratio rate was observed in men (1.26, 95%CI 1.17 to 1.35) and women (1.23, 95%CI, 1.15 to 1.32) in Goiânia, between 2003 and 2007. Conclusions: In Latin America, age effect was observed on incidence rates for colorectal, colon and rectal cancers. Besides, birth cohort effect was identified for recent cohorts in both genders for colorectal, colon and rectal cancers in Cali and Goiânia, and cohort effect for colorectal and colon cancers in both genders in Costa Rica; while in Quito a cohort effect was only observed for rectal cancer among men. Period effect was observed in Goiânia with increased ratio rate in 2003-2007.
https://journal.waocp.org/article_89367_ac6d367461fe759a72281c1fa26e9ec5.pdf
Colorectal Neoplasms
Age effect
Period effect. Cohort effect
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3429
3434
10.31557/APJCP.2020.21.11.3429
89368
Expression of microRNA-181a and microRNA-196b in Egyptian Pediatric acute Lymphoblastic Leukemia
Roxan Shafik
roxanezzat@yahoo.com
1
Nahed Abd El Wahab
nahedw7@hotmail.com
2
Marwa Mokhtar
mokhtarmarwa78@gmail.com
3
Maha El Taweel
maha15ayman@yahoo.com
4
Emad Ebeid
emadebeid@hotmail.com
5
Department of Clinical Pathology, NCI, Cairo University, Cairo, Egypt.
Department of Clinical Pathology, NCI, Cairo University, Cairo, Egypt.
Department of Clinical Pathology, NCI, Cairo University, Cairo, Egypt.
Department of Pediatric Oncology, NCI, Cairo University, Cairo, Egypt.
Department of Pediatric Oncology, NCI, Cairo University, Cairo, Egypt.
Background: Differential expression of miRNA provides important insights into pathogenesis of cancer including leukemia. Deregulation of microRNA may contribute to hematopoietic malignancies. In this study, we aimed to evaluate the role of miR-181a and miR-196b in acute lymphoblastic leukemia (ALL) and correlate their expression with clinical and laboratory data. Methods: The study was performed on bone marrow samples of 70 consecutive newly diagnosed pediatric (ALL) patients, of which 56 were evaluated for both miR-181a and miR-196b (all 70 for miR-181a) by real-time quantitative reverse transcriptase polymerase chain reaction (RT-qPCR). In addition, bone marrow from seven age and sex matched healthy controls derived from donors of bone marrow transplantation were assessed. Results: miR-181a expression was significantly up-regulated in ALL patients compared with healthy controls (p <0.001). However, miR-196b expression was significantly down-regulated in patients compared with healthy controls (p=0.038). Conclusion: Our results suggest that miR-181a has an oncogenic, while miR-196b has a tumor suppressive role in pediatric ALL patients. A finding which demonstrate the potential role of these microRNAs in pathogenesis of pediatric ALL. Also, estimation of their expression level may provide a tool for confirmation of a diagnosis of childhood ALL and could be a possible predictor of early relapse.
https://journal.waocp.org/article_89368_507add1d2f07df1585724e5950b51621.pdf
microRNA-181a
microRNA-196b
ALL
Pediatric
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3435
3439
10.31557/APJCP.2020.21.11.3435
89369
Overexpression of BASP1 Indicates a Poor Prognosis in Head and Neck Squamous Cell Carcinoma
Ashwin Jaikumarr Ram
ashwinjaikumar@gmail.com
1
Smiline Girija AS
smilinejames25@gmail.com
2
Vijayashree Priyadharsini Jayaseelan
viji26priya@gmail.com
3
Paramasivam Arumugam
paramasivam0103@gmail.com
4
Department of Microbiology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India.
Department of Microbiology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India.
BRULAC-DRC, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India.
BRULAC-DRC, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India.
Objective: Brain abundant membrane attached signal protein 1 (BASP1) was originally identified as a membrane and cytoplasmic protein. Recent studies have shown that BASP1 highly expressed in cancer and promoted the proliferation of cancer. However, the role of BASP1 in head and neck squamous cell carcinoma (HNSCC) is largely unknown. Here, we performed a systematic data analysis to examine whether BASP1 can function as prognostic marker in HNSCC. Methods: In this study, we used Oncomine, and UALCAN, databases to analyze the expression of BASP1 in HNSCC. We used Kaplan-Meier plotter to evaluate the effect of BASP1 on clinical prognosis. In addition, we also analyzed genetic alterations, interaction network, and functional enrichment of BASP1. Results: BASP1 mRNA expression level was remarkably increased in HNSCC than in normal tissues (P=1.624e-12). Moreover, high BASP1 expression was significantly related to poor survival (p=0.00056) in HNSCC patients. In addition, BASP1 gene amplified in 5% of HNSCC patients which contributes to the overexpression of BASP1. Conclusions: These findings suggest that BASP1 was frequently amplified which contributes to the overexpression of BASP1, thereby promoting HNSCC progression. Thus, these results indicate that BASP1 might serve as a biomarker to predict the progression and prognosis of HNSCC patients.
https://journal.waocp.org/article_89369_660951750a1869ae22aeadd942bda240.pdf
Head and neck squamous cell carcinomas (HNSCC)
BASP1
Prognostic marker
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2020-11-01
21
11
3441
3447
10.31557/APJCP.2020.21.11.3441
89370
Neoadjuvant Therapy with Drug Arglabin for Breast Cancer with Expression of H-Ras Oncoproteins
Sergazy Adekenov
arglabin@phyto.kz
1
Ainur Zhumakayeva
aynura_tuleuova@mail.ru
2
Vladimir Perminov
vp57_57@mail.ru
3
Bakhytzhan Bekmanov
bobekman@rambler.ru
4
Kayrolla Rakhimov
kdrakhimov@inbox.ru
5
JSC “International Research and Production Holding “Phytochemistry”, Karaganda, Republic of Kazakhstan.
JSC “International Research and Production Holding “Phytochemistry”, Karaganda, Republic of Kazakhstan.
Center of the Multidisciplinary Hospital No 3 of the Health Administration of the Karaganda Region, Kazakhstan.
Institute of General Genetics and Cytology, Kazakhstan. 5Asfendiyarov Kazakh National Medical University, Kazakhstan.
JSC “International Research and Production Holding “Phytochemistry”, Karaganda, Republic of Kazakhstan.
Backgrounds: In breast cancer, blocking of Ras signaling and inhibition of H-Ras is quite promising. H-Ras may become a target for farnesyl transferase inhibitors, and in combination with other immunohistochemical factors it will contribute to the progression of a breast tumor. Purpose: The aim of this study was to evaluate the effectiveness of neoadjuvant therapy for breast cancer with the inclusion of farnesyl transferase inhibitor, arglabin interfering with the expression and concentration of H-Ras oncoproteins. Methods: Depending on the presence of H-Ras oncoproteins after Western-blot hybridization, the patients were divided a negative and positive expression of H-Ras groups. Results: Correlation analysis of methods used for determining the expression ability and concentration of H-Ras oncoproteins (immunohistochemistry and Western-blot analysis) demonstrated substantial statistical relationship Rs=0.71, p=0.03. The H-Ras oncoproteins were absent in patients receiving either “Arglabin” or standard AC regimen. However, in the AC + Arglabin group, there was a varying degrees of positive concentration of H-Ras oncoproteins (Kruskal-Wallis=6.92; p=0.03). Conclusion: These results indicate that Arglabin attenuates H-Ras oncoproteins expression which is a promising therapeutic target for breast cancer.
https://journal.waocp.org/article_89370_6785737b7f2a9b93048d0b53d9f65042.pdf
arglabin
neoadjuvant therapy
farnesyl transferase