eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2687
2690
10.22034/APJCP.2018.19.10.2687
75282
Targeting Inflammation in Ovarian Cancer Through Natural Antioxidants, Potential Therapeutic and Preventive Implications
Mayank Singh
mayank.osu@gmail.com
1
Abhishek Shankar
2
K S Dhiman
3
Rajesh Kotecha
4
Goura Kishor Rath
5
Department of Medical Oncology (Lab), Dr BR Ambedkar Institute Rotary Cancer Hospital, All India Institute of Medical Sciences, Delhi, India.
Department of Preventive Oncology, Dr BR Ambedkar Institute Rotary Cancer Hospital, All India Institute of Medical Sciences, Delhi, India.
Director General, Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, Government of India, Delhi, India.
Secretary, Ministry of AYUSH, Government of India, Delhi, India.
Professor and Chief, Dr BR Ambedkar Institute Rotary Cancer Hospital & Head, National Cancer Institute - India, All India Institute of Medical Sciences, Delhi, India.
There is extensive experimental data to supportthe hypothesis that Reactive oxygen species (ROS)accumulation is one of the major underlying mechanismfor carcinogenesis. Many forms of cancer are associatedwith excessive accumulation of ROS including ovariancancer. It is now well understood that ROS productionshould be scavenged by antioxidant defence mechanism/DNA damage response of the human body to maintainnatural homeostasis (Kruk and Aboul-Enein, 2017).Dysregulation of this balance leads to many inflammatorydiseases including the high risk of developing cancer. HighROS concentration is associated with accumulation ofmutations which over the years leads to development ofcancer. Metabolic pathways including aerobic oxidationin mitochondria respiratory are the major source of ROSwhich in turn effect different signalling pathways (Krukand Aboul-Enein, 2017).
https://journal.waocp.org/article_75282_8743f8b6f484b13477338accfa2cde55.pdf
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2691
2694
10.22034/APJCP.2018.19.10.2691
69120
Amount of Narratives Used on Japanese Pro- and Anti-HPV Vaccination Websites: A Content Analysis
Tsuyoshi Okuhara
okuhara-ctr@umin.ac.jp
1
Hirono Ishikawa
hirono-tky@umin.ac.jp
2
Masahumi Okada
sokada-tuk@umin.ac.jp
3
Mio Kato
mkato-ctr@umin.ac.jp
4
Takahiro Kiuchi
tak-kiuchi@umin.ac.jp
5
Department of Health Communication, School of Public Health, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, Japan.
Department of Health Communication, School of Public Health, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, Japan.
Department of Health Communication, School of Public Health, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, Japan.
Department of Health Communication, School of Public Health, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, Japan.
Department of Health Communication, School of Public Health, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, Japan.
Background: HPV vaccination is an effective way in preventing cervical cancer. However, HPV vaccinationcoverage rate has fallen to only a few percent in Japan. Anti HPV-vaccination websites often use narratives of thosewho have experienced adverse reactions to HPV vaccine to enhance their persuasiveness. Scholars suggest using thesame medium to promote HPV vaccination online; e.g., presenting narratives of those who have experienced cervicalcancer. We examined the amount of narratives used on Japanese pro- and anti-HPV vaccination websites. Methods: Weconducted online searches using two major search engines (Googl.jp and Yahoo!.jp). Identified websites were classifiedas “pro,” “anti,” or “neutral” depending on their claims. Two independent raters coded the pro and anti websites andcounted the number of narratives of people who experienced side effects of HPV vaccine or cervical cancer. Results:A total 26% of anti websites posted narratives on side effects. No pro websites posted narratives about having cervicalcancer. Conclusion: Pro-HPV vaccination websites is recommended to post narratives to enhance the persuasivenessof their arguments.
https://journal.waocp.org/article_69120_3a6acde24ca02b8cbd6c5ddaa07abd16.pdf
Cervical cancer
human papillomavirus (HPV) vaccination
anti-vaccination movement
Internet
narrative
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2695
2700
10.22034/APJCP.2018.19.10.2695
69907
Cancer Patients During and after Natural and Man-Made Disasters: A Systematic Review
Hasan Abolghasem Gorji
hjafari381@gmail.com
1
Hamid Jafari
hjafari68@gmail.com
2
Mohammad Heidari
mo.heydari@yahoo.com
3
Bahar Seifi
seifi_b@yahoo.com
4
School of Health Management and Information Sciences, Iran University of Medical Sciences, Tehran, Iran.
Department of Health in Emergency and Disaster, School of Health Management and Information Sciences, Iran University of Medical Sciences, Tehran, Iran.
Department of Medical and Surgical, School of Nursing and Midwifery, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Department of Nursing, Faculty of Nursing and Midwifery, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
Background: Disasters affect all social functions. In particular, hospitals must mobilize their resources for response tomass injuries. This process can affected treatment of cancer patients and may resulted in delayed care. Considering theimportance of continuity of care for cancer patients, the aim of this systematic review was to identify challenges andpreparedness measures for cancer patients during and after disasters. Materials and Methods: This systematic reviewthat was conducted based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA)guidelines between January 2000 to December 2017. The keywords searched included: “disaster,” “emergency,”“crisis,” “disruptive event,” “technological disaster,” “natural disaster,” “cancer,” “cancer patient,” “chronic disease,”“continuity of care,” and “patient with chronic disease. “The Google Scholar, ISI Web of Science, Science Direct,PubMed and Scopus databases were searched. Results: After screening and review of article eligibility, seven wereincluded in the study. The selected articles were compared from several aspects. The results showed that most publications concerned all of the chronic diseases or all types of cancers. In addition breast cancer appeared a field of interest in disaster studies. Cancer studies in disaster management usually concentrated less on technological disasters. Conclusion: In aftermath of disasters, the concentration on measures to provide services for injuries and food, water and shelter, results in many challenges for cancer patients. Thus the health system must prepare cancer patients and caregivers for better responses to disasters. In addition, surge capacity must be considered in hospitals and other medical facilities to guarantee continuity of care.
https://journal.waocp.org/article_69907_401c595535596d11ee6f68bbc9ec54fe.pdf
Chronic Disease
Disasters
cancer
Social Support
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2701
2707
10.22034/APJCP.2018.19.10.2701
69905
Appraising of the Clinical Practice Guidelines Quality in the Non-Pharmacological Management of Chemotherapy-Induced Febrile Neutropenia; A Review
Shahin Salarvand
shsalarvand@lums.ac.ir
1
Simin Hemati
hemati@med.mui.ac.ir
2
Payman Adibi
adib@mui.ac.ir
3
Fariba Taleghani
shsalarvand@nm.mui.ac.ir
4
Social Determinant Health Research Center, Faculty of Nursing and Midwifery, Lorestan University of Medical Sciences, Khorramabad, Iran.
Department of Radiation Oncology , Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Integrative Functional Gastroenterology research center, Isfahan University of Medical Sciences, Isfahan, Iran.
Nursing and Midwifery Care Research Center, Faculty of Nursing and Midwifery, Isfahan University of Medical Sciences, Isfahan, Iran.
Background: Febrile neutropenia is a common and serious chemotherapy side effect, is associated with increasedmortality, morbidity, and treatment expenditures. Several CPGs (Clinical practice guidelines) have been releasedfor managing chemotherapy-induced febrile neutropenia. The aim of this study is Appraisal of the clinical practiceGuidelines quality in the management of chemotherapy-induced febrile neutropenia. Methods: A review study witha systematic search of the present CPGs for the management of chemotherapy-induced febrile neutropenia. Afterscreening the CPGs based on eligibility criteria, three CPGs were selected and 5 independent reviewers appraised themfor methodological quality by using the AGREE II Instrument. Results: Three CPGs were included; all of them wereevidence-based guidelines. The clarity of presentation domain scored the highest and the applicability domain has thelowest score among all domains of AGREE instrument and the rest of domains scored as descending respectively;Scope and purpose, stakeholder involvement, editorial independence, rigor of development. In general, the intra-classcorrelation coefficient (ICC) scores of all domains were very good according to the Landis and Koch’s scale, exceptthe Applicability domain scored as substantial. Conclusions: This study showed the quality of appraised CPGs. Threedomains of these CPGs based on the AGREE instrument scored less than other domains and were in relatively unfavorablestatus: applicability, rigor of development, editorial independence. Given the importance of these domains in guidelineimplementation, it is necessary to take actions for reducing these defects.
https://journal.waocp.org/article_69905_ae842699d2382f295913672357085f6f.pdf
Quality
clinical practice guidelines
AGREE II Instrument
chemotherapy-induced febrile neutropenia
cancer-related infections
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2709
2716
10.22034/APJCP.2018.19.10.2709
69906
Association between rs1862513 and rs3745367 Genetic Polymorphisms of Resistin and Risk of Cancer: A Meta-Analysis
Mohammad Hashemi
saeid.ghavami@gmail.com
1
Gholamreza Bahari
saeid.ghavami@gmail.com
2
Farhad Tabasi
tabasif@yahoo.com
3
Abdolkarim Moazeni-Roodi
ak.moazeni@gmail.com
4
Saeid Ghavami
saeid.ghavami@gmail.com
5
Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
Student Research Committee, Zahedan University of Medical Sciences, Zahedan, Iran.
Department of Clinical Biochemistry, School of Medicine, Iranshahr University of Medical Sciences, Iranshahr, Iran.
Children Hospital Research Institute of Manitoba, Biology of Breathing Theme, University of Manitoba, Winnipeg, Canada.
The present study aimed to assess any associations between resistin gene (RETN) polymorphisms and cancersusceptibility by conducting a meta-analysis. A comprehensive literature search was performed with PubMed, Web ofScience, Scopus and Google Scholar for relevant studies published before April 2018. For the rs1862513 polymorphism,data from 9 studies covering 1,951 cancer patients and 2,295 healthy controls were included in this meta-analysis. Pooledodds ratios (ORs) with 95% confidence intervals (CIs) were calculated. Our meta-analysis revealed that this RETNpolymorphism significantly increased the risk of cancer in codominant (OR=1.23, 95% CI= 1.01-1.50, p=0.04, CG vs CC;and OR=1.25, 95% CI= 1.03-1.53, p=0.03, GG vs CC), dominant (OR=1.19, 95% CI= 1.05-1.35, p=0.006, CG+GG vs CC), CI= 1.00-1.30, p=0.04, G vs C) inheritance genetic models. Stratification analysis by cancertype revealed that the rs1862513 variant significantly increased the risk of colorectal and breast cancer, and that canceroverall in Caucasians (OR=1.22, 95% CI= 1.04-1.43, p=0.02, CG+GG vs CC; OR=1.18, 95% CI= 1.04-1.34, p=0.01,G vs C). The data revealed no correlation between the rs3745367 polymorphism and cancer risk. Further well-designedstudies with larger sample sizes and different ethnicities are warranted to validate the present findings.
https://journal.waocp.org/article_69906_f3113b580cf8112856e8d1427c9cc5bc.pdf
Resistin
cancer
Polymorphism
Meta-analysis
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2717
2722
10.22034/APJCP.2018.19.10.2717
70385
Impact of an Educational Intervention Based on the BASNEF Model on Skin Cancer Preventive Behavior of College Students
Morad Ali Zareipour
1
Hassan Mahmoodi
2
Rohollah Valizadeh
rohvali4@gmail.com
3
Mousa Ghelichi Ghojogh
4
Monireh Rezaie Moradali
5
Fatemeh Zare
6
PhD student in Health Education and Health Promotion, Urmia Health Center, Urmia University of Medical Sciences, Urmia, Iran.
School of Health, Tabriz University of Medical Science, Tabriz, Iran.
Student Research Committee, Urmia University of Medical Sciences, Urmia, Iran.
Phd Student in Epidemiology, Department of Epidemiology, Faculty of Public Health, Shiraz University of Medical Sciences, Shiraz, Iran.
School of Nursing and midwifery, Islamic Azad University Urmia, Urmia, Iran.
PhD student in health education and health promotion, School of Health, Shahid Beheshti University of Medical Science, Tehran. Iran.
Background: Skin cancer is one of the most common cancers in the world but is largely preventable throughprotective behavior. The aim of this study was to evaluate the impact of an educational intervention basedon the BASNEF model on skin cancer prevention and protective behavior in midwifery students in Urmia.Methods: In this quasi-experimental study, the subject population comprised 100 midwifery students in two groups,study and control (n = 50 in each). The sample was collected using a stratified random sampling method. The datacollection tool was a multi-section questionnaire which included demographic questions, knowledge and structures(attitude, enabling factors, subjective norms, behavior and attitude). The educational intervention was carried out inthree 45-minute sessions. Data were collected during face-to-face discussions before the educational intervention andthree months thereafter and analyzed using paired t-test and independent t-test statistics. Results: The results showedthat after intervention, mean score of knowledge was significantly higher in the study group compared to the controlgroup. Significant improvement in mean scores for attitude, enabling factors, mental norms, and intent of behaviorwas limited to the study group Also, behavior for prevention of skin cancer was significantly better in the interventiongroup. Conclusions: The results of this study showed that the BASNEF model is effective for promotion of skin cancerprevention behavior.
https://journal.waocp.org/article_70385_9fe8c6ae93f7eaadb88786b8ccf844a1.pdf
BASNEF
cancer
educational
intervention
skin
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2723
2730
10.22034/APJCP.2018.19.10.2723
69112
Association of Elevated Peripheral Blood Micronucleus Frequency and Bmi-1 mRNA Expression with Metastasis in Iranian Breast Cancer Patients
Mahdieh Salimi
salimi@nigeb.ac.ir
1
Elaheh Eskandari
elaheeskandari87@yahoo.com
2
Department of Medical Genetics, Institute of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
Department of Medical Genetics, Institute of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
Background: In order to find cytogenetic and molecular metastasis biomarkers detectable in peripheral blood thespontaneous genomic instability expressed as micronuclei and Bmi-1 expression in peripheral blood of breast cancer(BC) patients were studied in different stages of the disease compared with unaffected first-degree relatives (FDRs)and normal control. Methods: The Cytokinesis Block Micronuclei Cytome (CBMN cyt) and nested real-time ReverseTranscription-Polymerase Chain Reaction (RT-PCR) assays, were respectively used to measure genomic instability andBmi-1 gene expression in 160 Iranian individuals comprised of BC patients in different stages of the disease, unaffectedFDRs and normal control groups. Result: The frequency of micronuclei and Bmi-1 expression were dramatically higherin distant metastasis compared with non-metastatic BC. In spite of micronucleus frequency with no association withlymph node (LN) involvement and hormone receptor status, the Bmi-1 expression level was higher in LN positive andtriple negative patients. Conclusion: Our results indicate that increased genomic instability expressed as micronuclei andhigher Bmi-1 expression in peripheral blood are associated with metastasis in breast cancer. Therefore implementationof micronucleus assay and Bmi-1 expression analysis in blood as possible cytogenetic and molecular biomarkers inclinical level may potentially enhance the quality of management of patients with breast cancer.
https://journal.waocp.org/article_69112_1ca3749ae3a6b03b9ade2dd125ccfd4a.pdf
breast cancer
Gene expression
micronucleus
metastasis biomarkers
oxidative stress
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2731
2735
10.22034/APJCP.2018.19.10.2731
69073
HLA-G Expression in Tumor Tissues and Soluble HLA-G Plasma Levels in Patients with Gastrointestinal Cancer
Shirin Farjadian
farjadsh@sums.ac.ir
1
Maryam Tabebordbar
tabebm15@yahoo.com
2
Maral Mokhtari
mokhtarim@sums.ac.ir
3
Akbar Safaei
asafaei@sums.ac.ir
4
Mahyar Malekzadeh
mahyar.malekzadeh@gmail.com
5
Abbas Ghaderi
ghaderia@sums.ac.ir
6
Department of Immunology, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Immunology, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran.
Institute for Cancer Research, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Immunology, Shiraz University of Medical Sciences, Shiraz, Iran.
Background: Overexpression of human leukocyte antigen G (HLA-G) and increased plasma levels of solubleHLA-G (sHLA-G) have been reported in different human malignancies, and are believed to be involved in tumor immuneevasion. Objectives: This study was designed to evaluate the expression of HLA-G in tumor tissues and the plasmalevels of sHLA-G in patients with gastrointestinal cancer, and to determine their associations with clinicopathologicalfactors. The link between Helicobacter pylori infection and increased HLA-G expression or sHLA-G levels was alsoinvestigated in patients with gastric cancer. Methods: HLA-G expression was investigated in tumor tissues from 100patients with gastric and colorectal adenocarcinoma using immunohistochemistry test, and plasma levels of sHLA-Gwere measured in 82 patients with ELISA method. The presence of H. pylori genome was investigated in tumortissues from 25 patients with gastric cancer by PCR method. Results: HLA-G expression was observed in 43% ofcolorectal cancers and 34.6% of gastric cancers, and was not related with any of the clinicopathological factors. Therewas a significant correlation between increased sHLA-G level and stage I tumors. Eight of 25 (32%) gastric cancerspecimens were positive for H. pylori, of which 3 samples were positive for HLA-G. Soluble HLA-G levels were abovethe cut-off value in all H. pylori-positive patients. Conclusion: Plasma levels of sHLA-G were significantly increasedin our patients with a sensitivity of 89% and a specificity of 62%. Soluble HLA-G level can be considered a usefulindicator for the early diagnosis of gastric and colorectal adenocarcinoma.
https://journal.waocp.org/article_69073_0b81fd0f2fb7f4b2ada248eae6b987f2.pdf
HLA-G
sHLA-G
colorectal cancer
Gastric cancer
Helicobacter pylori
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2737
2744
10.22034/APJCP.2018.19.10.2737
69119
Orthosiphon stamineus Extracts Inhibits Proliferation and Induces Apoptosis in Uterine Fibroid Cells
Norzilawati Pauzi
norzilawatipauzi@yahoo.com
1
Khamsah Suryati Mohd
khamsahmohd@gmail.com
2
Nor Hidayah Abdul Halim
iey_da2412@yahoo.com
3
Zhari Ismail
zhari@usm.my
4
Faculty of Bioresources and Food Industry, University Sultan Zainal Abidin, Besut Campus, Besut, Terengganu, Malaysia.
Faculty of Bioresources and Food Industry, University Sultan Zainal Abidin, Besut Campus, Besut, Terengganu, Malaysia.
Faculty of Bioresources and Food Industry, University Sultan Zainal Abidin, Besut Campus, Besut, Terengganu, Malaysia.
School of Pharmaceutical Science, University Sains Malaysia, Penang, Malaysia.
Objectives: The effects of water and 50% ethanolic-water extracts of Orthosiphon stamineus Benth (OS) on cellproliferation and apoptotic activity against uterine leiomyosarcoma (SK-UT-1) cells were investigated. Methods:Anti-proliferation effect was evaluated through cell cycle analysis whereas apoptotic activity was determined viascreening and quantifying using fluorescence microscopy and flow cytometric analysis, respectively. The effect ofextracts on molecular mechanism was studied using real-time reverse transcription polymerase chain reaction andWestern blotting. Results: Cell cycle flow cytometric analysis showed the induction of cell cycle arrests were behavesin a p53-independent manner. The examination using fluorescence microscopy and Annexin V flow cytometry revealedthe presence of morphological features of apoptotic bodies. Downregulation of anti-apoptotic gene (Bcl-2) supportsthe apoptotic activity of OS extracts although poorly induce PARP-1 cleavage in Western blot analysis. The extractsalso inhibit the SK-UT-1 growth by suppressing VEGF-A, TGF-β1 and PCNA genes, which involved in angiogenesisand cell proliferation. Conclusion: This study demonstrates that O. stamineus extracts are able to inhibit proliferationand induced apoptosis of uterine fibroid cells and is worth further investigation.
https://journal.waocp.org/article_69119_a7e83405738ce760358f177bbbd50571.pdf
Orthosiphon stamineus Benth
SK-UT-1 cells
Apoptosis
growth factor
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2745
2750
10.22034/APJCP.2018.19.10.2745
70390
Concurrent Chemo- Radiobrachytherapy with Cisplatin and Medium Dose Rate Intra- Cavitary Brachytherapy for Locally Advanced Uterine Cervical Cancer
Mahdi Aghili
aghili@tums.ac.ir
1
Bahram Andalib
andalib@yahoo.com
2
Zhaleh Karimi Moghaddam
zhkarimi@zums.ac.ir
3
Afsaneh Maddah Safaei
afsan1980@ymail.com
4
Farnaz Amoozgar Hashemi
amoozfar@tums.ac.ir
5
Nima Mousavi Darzikolaei
mousavi.nima@yahoo.com
6
Radiation Oncology Research Center, Department of Radiation Oncology, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran.
Radiation Oncology Research Center, Department of Radiation Oncology, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran.
Department of Radiation Oncology, Zanjan University of Medical Sciences, Zanjan, Iran.
Radiation Oncology Research Center, Department of Radiation Oncology, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran.
Radiation Oncology Research Center, Department of Radiation Oncology, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran.
Radiation Oncology Research Center, Department of Radiation Oncology, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran.
Objective: Cervical carcinoma is the third most common gynecologic cancer, after ovarian and uterine cancersin Iran. The aim of this study was to evaluate the efficacy (response rate) and toxicity of adding Medium Dose Rate(MDR) brachytherapy with concurrent chemotherapy to External Beam Radiotherapy (EBRT) for the treatment oflocally advanced uterine cervical carcinoma. Methods: This phase I-II study was conducted in 2007-2008 at the cancerinstitute, Tehran University of Medical Sciences. Patients were treated with pelvic EBRT (50 Gy in 25 Fraction) withconcomitant chemotherapy to obtain tumor shrinkage and permit optimal intra-cavitary placement. One week after thecompletion of EBRT, patients were treated by 12 Gy MDR Intra-cavitary brachytherapy for two periods of one daywith a one week interval and concomitant platinum based chemotherapy. Response rate was evaluated by gynecologicphysical examination and pelvic MRI +- GD within three months of treatment. Acute and late toxicity were assessedusing Radiation Therapy Oncology Group criteria. Results: A total of 33 patients with locally advanced cervical cancerwere treated according to the above described treatment protocol. The patients mean age was 53.2 (range 31–78) years.Three months after the completion of treatment, the complete clinical, pathologic and radiologic response rate accordingto WHO-criteria was 81.8% (27 patients). Six cases had a partial response or stable disease. After 48 months, averagedisease free survival periods were 45.1, 23.0, 33.4 and 8 months for stage IIB, IIIA, IIIB and: IVA lesions (according toThe International Federation of Gynecology and Obstetrics staging system). The most frequently observed side effectswere leukopenia, anemia, proctitis, cystitis, nausea and vomiting (mostly grade 1 and 2). Conclusion: Concomitantbrachytherapy and chemotherapy with platinum compounds can be well tolerated and is effective in the treatment oflocally advanced cervical cancer.
https://journal.waocp.org/article_70390_4346eadb688062bee03912c3ec969c82.pdf
Uterin Cervical Cancer
MDR
Brachytherapy
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2751
2755
10.22034/APJCP.2018.19.10.2751
70479
Mediating Factors in Early Diagnosis of Breast Cancer: from Initial Changes in Health to Breast Cancer Detection
Nasrin Fouladi
foladi_n@yahoo.com
1
Farhad Pourfarzi
f.pourfarzi@arums.ac.ir
2
Aida Daneshian
aidadaneshian@yahoo.com
3
Sara Alimohammadi
sarahalimohammadi@yahoo.com
4
Community Medicine, Faculty of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
Community Medicine, Faculty of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
Community Medicine, Faculty of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Background: Breast cancer is one of the most frequently occurring cancers in women throughout the world. InIran, according to available reports, 70% of patients with breast cancer are detected at the advanced stages of thedisease. Materials and Methods: This study is of descriptive-analytic cross-sectional type.160 female patients wasselected. The data in this study was collected via face to face interview using a questionnaire based on the Anderson’sdelay model. Data were analyzed using SPSS 22 and the significance level was considered to be 0.05. Results: Theresults showed that presence of mass was the first symptom in many of women, i.e., 76 cases (47.5%). not only thepresence of mass in the breast, but also any other symptom, hadn’t been taken as a serious problem initially by women.Non-improvement of the symptoms and exacerbation of the symptoms was considered as a symptom of illness bypatients. After considering the changes as the symptoms of illness, patients had tried to treat the disease through differentmethods of self-treatment. The failure of the self-treatment in controlling the symptoms, had directed the patientstoward seeking for medical services. Out of 160 patients, 49 patients (39.6%) changed the time of their appointmentwith doctor. 110 cases (68%) out of 160 patients, rather than initiating relevant diagnostic procedure related to thedisease, had received non-specific and non-related therapeutic measures. Conclusion: Pondering on the results yieldsthat patient-related delays, resulting from their lack of awareness of the disease, cultural factors, and fear, can onlyplay an important role in postponement of visiting a physician, but in the diagnosis of disease it is the inability ofgeneral practitioners in performing differential diagnoses, and making request for related diagnostic measures whichcan significantly increase the lag time until the onset of the main treatment.
https://journal.waocp.org/article_70479_ba8ea64fd0588df064efdd5a974d20c5.pdf
breast cancer
delay
Anderson model
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2757
2764
10.22034/APJCP.2018.19.10.2757
70489
Effects of ABCC2 and SLCO1B1 Polymorphisms on Treatment Responses in Thai Metastatic Colorectal Cancer Patients Treated with Irinotecan-Based Chemotherapy
Apatsara Treenert
yoyok4913@gmail.com
1
Nutthada Areepium
nutthada.a@pharm.chula.ac.th
2
Suebpong Tanasanvimon
surbpong.@yahoo.com
3
Department of Pharmacy Practice, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand.
Department of Pharmacy Practice, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand.
Department of Medical Oncology Unit, Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Purpose: Irinotecan is an anticancer medicine which is used mostly in metastatic colorectal cancer (mCRC) treatmentas second or third line chemotherapy. Several factors affect its efficacy and toxicity, including pharmacogenomics.This study aimed to investigate the impacts of ABCC2 and SLCO1B1 polymorphisms on treatment responses inirinotecan-based chemotherapy in 49 Thai mCRC patients. Materials and Methods: Forty-nine participants withmCRC enrolled in this study received irinotecan-based chemotherapy from January to June 2017. Genotypic analyses ofABCC2 (C>T, rs717620) and SLCO1B1 (A>G, rs2306283) were performed. Treatment responses were evaluated afterat least three cycles of chemotherapy were given. Results: Allele frequencies of ABCC2 (C>T) and SLCO1B1 (A>G)were found at 18.37% and 78.57%, respectively. Neither was associated with treatment responses. However, combinedgenotypes of ABCC2 and SLCO1B1 tended to be associated with clinical benefits in terms of partial responses (PR) andstable disease (SD). All patients (100%) with at least one variant allele of SLCO1B1 and ABCC2 were in a PR or SDgroup, while patients with other genotypes had progressive disease (PD) at 45.5% to 70%, (p = 0.059). Conclusion:The combined effect of ABCC2 and SLCO1B1 polymorphisms tended to be associated with treatment responses inirinotecan-based treated mCRC patients. Therefore, such polymorphisms could be factors impacting inter-individualvariation of irinotecan efficacy in Thai mCRC patients.
https://journal.waocp.org/article_70489_72108f996ed84e8975e1610f97e26a29.pdf
Keywords: Metastatic Colorectal cancer
ABCC2
SLCO1B1
Irinotecan
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2765
2770
10.22034/APJCP.2018.19.10.2765
69087
Two Different Methods of Region-of-Interest Placement for Differentiation of Benign and Malignant Breast Lesions by Apparent Diffusion Coefficient Value
Masoumeh Gity
p_gity@yahoo.com
1
Behnaz Moradi
b.moradi80@gmail.com
2
Rasool Arami
dr.arami93@gmail.com
3
Ali Kheradmand
kheradmand@tums.ac.ir
4
Mohammad Ali Kazemi
ma-kazemi@sina.tums.ac.ir
5
Department of Radiology, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran.
Department of Radiology, Women’s Yas Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Department of Radiology, Sina Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Department of surgery, Cancer institute, Breast cancer research center, Tehran University of Medical Sciences, Tehran, Iran.
Department of Radiology, Amiralam Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Purpose: We aimed to investigate the influence of different methods of region-of-interest (ROI) placement onapparent diffusion coefficient (ADC) values in breast tumours and their accuracy in differentiating benign versusmalignant tumors in mass and nonmass lesions. Methods and Materials: In this prospective study, 79 patients with98 breast lesions, from 2015 until 2017, were investigated by 1.5-T breast MRI. Histopathology evaluation were donefor all malignant lesions and most of the benign ones. ADC values were measured in normal breast tissue and by twoways of ROI placement in the breast lesions (mass and non-mass): 1- ROI covering the whole lesion, 2- ROI in thehighest part (most restricted area) of the lesion in DWI images. The accuracy of these two approaches were compared.Results: The age range was 17-68 years with mean age 43.3 ± 9.9 years. 49% of the lesions were benign and 51% oftumors were malignant. Our results revealed that the measured ADC values in normal breast tissue were higher thanbreast lesions (P≤0.01). Appropriate cut off determination in non-mass was not valid by both methods, but in mass inthe first way was 1.45×10 -³mm²/s and in the most restricted part was 1.16×10-³ mm²/s. ADC values differed significantlybetween the two ways of ROI placement in mass lesions (P<.001). Most restricted part ADC showed the best diagnosticperformance in mass lesions with area under curve 0.88 versus 0.82. Conclusion: ROI placement has significant impacton the meseaured ADC values of breast lesions and ROIs in most restricted parts were more accurate than whole-lesionROIs. Cut-off values differed significantly based on the methods of measurement.
https://journal.waocp.org/article_69087_5f0bbae599526f0b7ce72ae579d87822.pdf
breast cancer
Magnetic resonance imaging
Diffusion magnetic resonance imaging
Apparent diffusion coefficient
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2771
2775
10.22034/APJCP.2018.19.10.2771
73833
Automatic Segmentation of MRI Images in Dynamic Programming Mode
Mariya Y Marusina
marusina_m@mail.ru
1
Elizaveta A Karaseva
lzvt.krsv@gmail.com
2
ITMO University, Saint-Petersburg, Russia.
ITMO University, Saint-Petersburg, Russia.
Objective: Purpose of this work was to develop methods contour and volume of areas of interest definition intomographic images of the breast. Methods: The study included images of the breast of 13 patients obtained on an openelectronic resource The Breast-MRI-NACT-Pilot image collection. Statistical processing of the data was carried out, thereliability of the results of calculating the volumes of the breast areas was estimated, a visual evaluation of the obtainednumerical values was provided – a linear graph. Result: A program for automatic determination of breast volume andvolume of pathology has been developed and tested. For segmenting areas of the breast, a threshold segmentation anda managed watershed method programs were written in Matlab package. The developed programs allowed to obtainreliable data when processing MRI images of the breast of 13 patients. Results of using Hurst exponent show that inthe case of a pathology, the exponent is less than 0.4, and for the tissue without pathology the Hurst index is greaterthan 0.4. This method is implemented in dynamic programming mode, which allows to adjust the algorithm for a taskof examining images. Conclusion: The developed methods of definition of contours and calculating volumes allow anautomatic quantitative evaluation of the ratio of the volumes of different identified areas of interest in the postprocessingof MRI images. Also, the results have established that it is possible to use the Hurst exponent as an additional tool foridentifying pathologies in areas of interest.
https://journal.waocp.org/article_73833_a69d87c0d3222b1d59798880f15772d1.pdf
Segmentation
MRI
Postprocessing
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2777
2787
10.22034/APJCP.2018.19.10.2777
69069
The Effect of Educational Intervention Based on Health Belief Model and Social Support on the Rate of Participation of Individuals in Performing Fecal Occult Blood Test for Colorectal Cancer Screening
Yousef Gholampour
yousefgholampour@gmail.com
1
Atefeh Jaderipour
ajaderipour@gmail.com
2
Ali Khani Jeihooni
khani_1512@yahoo.com
3
Seyyed Mansour Kashfi
smkashfi@yahoo.com
4
Pooyan Afzali Harsini
pooyanafzali@gmail.com
5
Department of Internal Medicine, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran.
Student of Medicine, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran.
Department of Public Health, School of Health, Fasa University of Medical Sciences, Fasa,Iran.
Department of Public Health, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Public Health, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Background and Aim: Among the screening tests for colorectal cancer, fecal occult blood test (FOBT) is important incomparison other methods due to its ease of use and low cost.The aim of this study is to survey the effect of educationalintervention based on health belief model and social support on the rate of participation of individuals in performingfecal occult blood test for colorectal cancer screening among men who referred to the health centers in FasaCity, Farsprovince, Iran. Materials and Methods: In this quasi-experimental study, 200 men (100 in experimental group and100 in control group) in FasaCity, Fars province, Iranwere selected in 2017. A questionnaire consisting of demographicinformation, knowledge, HBM constructs (perceived susceptibility, severity, benefits, barriers, self- efficacy and cuesto action) and social support was used to measure the rate of participation of individuals in performing Fecal OccultBlood Test for colorectal cancer screening before and three months after the intervention. Data were analyzed usingSPSS22 viadescriptive and inferential statistics, paired t-test, Mann-Whitney, Chi-square, and independent t-test ata significance level of 0.5. Results: The mean age of the men was 63.18 ± 8.25 years in the experimental group and65.11 ± 7.66 years in the control group. Three months after the intervention, the experimental group showed a significantincrease in the knowledge, perceived susceptibility, perceived severity, perceived benefits, Self-efficacy, cues to action,social support and the level of referrals (participation) of subjects for FOBTcompared to the control group. Conclusion:This study showed the effectiveness of HBM constructs and social support in adoption of the level of participation ofsubjects for FOBTin men. Hence, these models can act as a framework for designing and implementing educationalinterventions for undergoing FOBT.
https://journal.waocp.org/article_69069_89468dac21726e1efd7af4f582701402.pdf
Fecal Occult Blood Test
Men
Health Belief Model
Social Support
colorectal cancer
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2789
2794
10.22034/APJCP.2018.19.10.2789
69798
An Efficient Method for Brain Tumor Detection Using Texture Features and SVM Classifier in MR Images
Kavin Kumar K
kavinece07@gmail.com
1
Meera Devi T
tmeeradevi@gmail.com
2
Maheswaran S
mmaheswaraneie@gmail.com
3
Department of Electronics and communication Engineering, Kongu Engineering College, Perundurai, Erode -638 060, Tamil Nadu, India.
Department of Electronics and communication Engineering, Kongu Engineering College, Perundurai, Erode -638 060, Tamil Nadu, India.
Department of Electronics and communication Engineering, Kongu Engineering College, Perundurai, Erode -638 060, Tamil Nadu, India.
Objective: Detection and classification of abnormalities in Magnetic Resonance (MR) brain images in medical fieldis very much needed. The proposed brain tumor classification system composed of denoising, feature extraction andclassification. Noise is one of the major problems in the medical image and due to that retrieval of useful informationfrom the image is difficult. The proposed method for denoising an image is PURE-LET transform. Methods: Thismethod preserves the diagnostic property of the images. In feature extraction, combination of Modified Multi-TextonHistogram (MMTH) and Multi-Texton Microstructure Descriptor (MTMD) is used and then Gray Level Co-occurrenceMatrix (GLCM) and Gray Level Run Length Matrix (GLRLM)are used to extract the feature from the image tocompare performance. In classification, classifiers like Support Vector Machine (SVM), K Nearest Neighbors (KNN)and Extreme Learning Machine (ELM)are trained by the extracted features and are used to classify the images. Result:The performance of feature extraction methods with three different classifiers are compared in terms of the performancemetrics like sensitivity, specificity, and accuracy. Conclusion: The result shows that the combination of MMTH andMTMD with SVM shows the highest accuracy of 95%.
https://journal.waocp.org/article_69798_9a9e7d28160b324a63df709626b2b4cc.pdf
Denoising
PURE-LET
Feature extraction - MMTH
MTMD
GLCM
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2795
2802
10.22034/APJCP.2018.19.10.2795
69799
Clinical, Epidemiological and Histopathological Aspects in Patients with Hepatocellular Carcinoma Undergoing Liver Transplantation
Nathalia Martines Tunissiolli
nathaliatunissiolli@yahoo.com
1
Márcia Maria Urbanin Castanhole-Nunes
marcinha.uc@gmail.com
2
Érika Cristina Pavarino
erika@famerp.br
3
Renato Ferreira da Silva
renatosilva@famerp.br
4
Rita de Cássia Martins Alves da Silva
ritasilva50@gmail.com
5
Eny Maria Goloni Bertollo
eny.goloni@famerp.br
6
Research Unit of Genetics and Molecular Biology (UPGEM),Sao Jose do Rio Preto Medical School (FAMERP), Sao Jose do Rio Preto, Brazil.
Research Unit of Genetics and Molecular Biology (UPGEM),Sao Jose do Rio Preto Medical School (FAMERP), Sao Jose do Rio Preto, Brazil.
Research Unit of Genetics and Molecular Biology (UPGEM),Sao Jose do Rio Preto Medical School (FAMERP), Sao Jose do Rio Preto, Brazil.
Department of Surgery and Liver Transplantation, Hospital de Base/ FUNFARME, Sao Jose do Rio Preto Medical School (FAMERP), Sao Jose do Rio Preto, Brazil.
Department of Surgery and Liver Transplantation, Hospital de Base/ FUNFARME, Sao Jose do Rio Preto Medical School (FAMERP), Sao Jose do Rio Preto, Brazil.
Research Unit of Genetics and Molecular Biology (UPGEM),Sao Jose do Rio Preto Medical School (FAMERP), Sao Jose do Rio Preto, Brazil.
Background: Hepatocellular Carcinoma (HCC) is the primary liver cancer with high incidence and mortality rates.Currently one of the major etiologies for liver disease, HCC and liver transplantation is nonalcoholic fatty liver disease(NAFLD). The aim of the present study was to evaluate the epidemiological, histopathological and clinical aspects ofHCC transplant patients, with emphasis on NAFLD etiology. Methods: This study included all HCC patients submittedto liver transplantation from 2010 to 2016 of the University Reference Center. The analyzed variables were age, gender,ethnicity, causes that led to liver transplantation, alpha-fetoprotein (AFP) dosage, histological aspects, recurrence,survival and NAFLD. Results: A total of 60 patients were included in the study being 80% men with a mean age of58.3 ± 10.6 years. All patients were cirrhotic. The causes that led to the transplantation were the presence of the hepatitisC virus (HCV) (56.6% of the patients), an association of the virus with alcohol (20%), the presence of the hepatitis Bvirus (HBV) (20%), alcoholic liver disease (ALD) (50.9%) and NAFLD (25%). Of the latter, eight were diagnosedpre-transplantation and seven were NAFLD carriers without a previous diagnosis. Regarding the Edmondson-Steinerhistological classification, 58.5% of the patients were classified as grade ≤ II. Conclusions: There is predominance ofmale patients with a mean age of 58.3 years. Degree ≤ II is the most frequent to the Edmondson-Steiner histologicalclassification in the evaluated casuistic. HCV, ALD and NAFLD is the most common etiological agents found in thestudy. The (high) underestimated prevalence of NAFLD in the pre-transplanted patients is due to the fact that all patientspresented cirrhosis, masking NAFLD signals.
https://journal.waocp.org/article_69799_bae54b20a182cc5ce864d19baa8bf5a6.pdf
Liver cancer
non-alcoholic fatty liver disease
liver transplantation
Epidemiology
risk factors
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2803
2806
10.22034/APJCP.2018.19.10.2803
69800
Hematologic Toxicity of Conformal Radiotherapy and Intensity Modulated Radiotherapy in Prostate and Bladder Cancer Patients
Marcin Miszczyk
marcinmmiszczyk@gmail.com
1
Wojciech Majewski
wmajewski1@poczta.onet.pl
2
Department of Radiotherapy Planning, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Poland.
Department of Radiotherapy, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Poland.
Background: The purpose of this study was to compare hematologic adverse effects and hematologic toxicity(HT) of pelvic irradiation in patients treated with conformal radiotherapy (CRT) and intensity modulated radiotherapy(IMRT) for radical treatment of prostate and bladder cancer. Methods: A group of 115 patients with prostate or bladdercancer treated with definitive radical radiotherapy was evaluated retrospectively. Blood test were taken before andafter treatment comprising of following indices: white blood cells (WBC) hemoglobin (HGB), red blood cell (RBC),lymphocyte (LC), neutrophil (NC) and platelet (PLT) count. Patients were divided into several subgroups and the datawas evaluated statistically using absolute and relative values. Results: There was a statistically significant differencein WBC (p=0.007), NC (p=0.031) and PLT (p=0.026) count decrease (absolute values) after treatment, between twotreatment methods (CRT and IMRT), all in favor of IMRT. The relationship still proves to be significant regardingWBC (p=0,02) and (NC) (p=0,049) after presenting the data as relative percentage loss of starting value. Howeverusing Common Terminology Criteria for Adverse Effects (CTCAE), PLT count toxicity was more common in IMRTgroup (p=0.045). Conclusion: IMRT in comparison to CRT in bladder and prostate cancer patients is associated with alesser absolute and relative decrease of hematologic indices. The hematologic effect of radiation was observed mainlyregarding LC. Patients treated with IMRT suffered from significantly lesser decrease in relative and absolute valuesof WBC and NC. The mean of absolute PLT decrease count was lower in IMRT group; however, toxicity according toCTCAE was slightly more prevalent in IMRT group.
https://journal.waocp.org/article_69800_35e528dd333a640f62d5328ae31a88be.pdf
Prostatic Neoplasms
Urinary Bladder Neoplasms
Radiotherapy Intensity-Modulated
Radiotherapy Conformal
Hematologic Toxicity
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2807
2813
10.22034/APJCP.2018.19.10.2807
69066
High Prevalence of Human Papillomavirus Types 56 and 70 Identified in the Native Populations of Sabah, Malaysia
Nur Ezzah Sainei
ezzahsainei@hotmail.com
1
Vijay Subbiah Kumar
vijay@ums.edu.my
2
Yeung Sing Chin
acys77@hotmail.com
3
Falah A M Salih
muhamadsalih.falah@gmail.com
4
Department of Biomedical Sciences and Therapeutics, Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, Kota Kinabalu, Malaysia.
Biotechnology Research Institute, Universiti Malaysia Sabah, Kota Kinabalu, Malaysia.
Department of Obstetrics and Gynaecology, Sabah Women and Children Hospital, Kota Kinabalu, Malaysia.
Department of Biomedical Sciences and Therapeutics, Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, Kota Kinabalu, Malaysia.
Background: Cervical cancer is currently the third most common female cancer in Malaysia , with the humanpapillomavirus (HPV) considered as one of the important contributory factors. This study was conducted to determineHPV prevalence, its genotype distribution, and other potential risk factors among women in Kota Kinabalu, Sabahin order to evaluate the likely efficacy of current HPV vaccines in the local population. Methods: A total of 240cervical samples were collected and subjected to DNA extraction, PCR amplification using the MY09/MY11 primerpair, and restriction fragment length polymorphism (RFLP) for HPV detection and genotyping. Sociodemographic,clinical, and behavioural data were also collected via questionnaires. Results: The prevalence of HPV infection was9.6%. The most common HPVs among 13 genotypes were high-risk HPV-56 (16.7%) and probable high-risk HPV-70(16.7%) followed by HPV-16, -58, -53, -61, -33, -59, and -66 (in decreasing order of prevalence) including the raregenotypes: HPV-62, -81, -82 and -84. Statistical analyses using logistic regression models showed that HPV infectionwas significantly associated with employment (OR 4.94; CI 1.58-15.40) and education at secondary/high school level(OR 0.13; CI 0.03-0.62). Conclusion: Distribution of HPV genotypes in Sabah indicated a high prevalence of HPV-56and -70 which are among the rare HPV types in West Malaysia and merit consideration in future strategies for HPVvaccination specifically for local Sabahan women.
https://journal.waocp.org/article_69066_8b18628d06c2f590c0d32a55c794339d.pdf
Cervical cancer
human papillomavirus
Prevalence
risk factor
Sabah
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2815
2820
10.22034/APJCP.2018.19.10.2815
70491
Reliability and Construct Validity of the Malay Version of Theory of Planned Behaviour (TPB) for Smoking Cessation
Nur-Hasanah Ruslan
nur_hasanahruslan@yahoo.com
1
Siti Munira Yasin
drsitimy@salam.uitm.edu.my
2
Mohamad Rodi Isa
mrodi@salam.uitm.edu.my
3
Nadzimah Mohd Nasir
nadzimah@salam.uitm.edu.my
4
Theresa Ahing
theresaahing@sarawak.uitm.edu.my
5
Mohd Hamim Rajikin
hamim400@salam.uitm.edu.my
6
Faculty of Sport Science and Recreation, Universiti Teknologi MARA Cawangan Pahang, Bandar Pusat Jengka, Malaysia.
Faculty of Medicine, Universiti Teknologi Mara Sg. Buloh, Selangor, Malaysia.
Faculty of Medicine, Universiti Teknologi Mara Sg. Buloh, Selangor, Malaysia.
Faculty of Medicine, Universiti Teknologi Mara Sg. Buloh, Selangor, Malaysia.
Faculty of Sport Science and Recreation, Universiti Teknologi MARA Kota Samarahan, Sarawak, Malaysia.
Faculty of Medicine, Universiti Teknologi Mara Sg. Buloh, Selangor, Malaysia.
Objective: The aim of this study was to evaluate the psychometric properties of the Malay translated version ofthe Theory of Planned Behavior (TPB) intention to quit smoking questionnaire. Methods: A cross-sectional studywas performed involving 185 male smokers. The forward-backward translation procedure was adopted to translate thequestionnaire from English to Malay. The internal consistency and stability were assessed using Cronbach’s alpha and acorrelation analysis and Exploratory Factor Analysis was conducted. Result: The translated questionnaire showed goodinternal consistency with Cronbach’s alpha values of 0.86, 0.64, 0.74 and 0.90 for each of the four respective factors.The test-retest reliability revealed acceptable stability, with Spearman’s correlation coefficients ranging from low tomoderate (r>0.30-0.50) and a satisfactory inter class correlation coefficient (ICCs). The construct validity achieved anacceptable factor loading for each construct which ranged from 0.40 to 0.90. Conclusion: The current study providedpsychometric evidence for an appropriate, reliable and valid tool of TPB Malay version. This questionnaire could beapplied in evaluating smoking cessation interventions in Malaysia.
https://journal.waocp.org/article_70491_8155dd136f80dfbc05af6c443c06fb43.pdf
Theory of Planned Behavior
translation
Validation
smoking
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2821
2829
10.22034/APJCP.2018.19.10.2821
69070
Bio-Effects of TiO2 Nanoparticles on Human Colorectal Cancer and Umbilical Vein Endothelial Cell Lines
Nasim Rahmani Kukia
nasimrahmani86@gmail.com
1
Yousef Rasmi
yrasmi@gmail.com
2
Ardashir Abbasi
abbasi.ardashir1987@gmail.com
3
Nana Koshoridze
nana.koshoridze@yahoo.com
4
Alireza Shirpoor
ashirpoor@gmail.com
5
Giorgi Burjanadze
burjanadze.giorgi@gmail.com
6
Ehsan Saboory
esaboory@gmail.com
7
Department of Biochemistry, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
Department of Biochemistry, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Department of Biology, Faculty of Exact and Natural Sciences, Ivane Javakhishvili Tbilisi State University, Georgia.
Department of Physiology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
Department of Biology, Faculty of Exact and Natural Sciences, Ivane Javakhishvili Tbilisi State University, Georgia.
Neuroscience Research Center, Urmia University of Medical Sciences, Urmia, Iran.
Background: Due to the possible biomedical potential of nanoparticles, titanium dioxide nanoparticles (TiO2 NPs)have received great attention in cancer research. Although selectivity of cytotoxicity with TiO2 NPs in various cells isclinically significant comparisons of cancer and non-cancer cells have been limited. Therefore, we here studied exposureto TiO2 NPs in colorectal cancer cells (CRCs) and human umbilical vein endothelial cells (HUVECs). Methods: Aftercharacterization of TiO2 NPs, culture and treatment of cells (HCT116, HT29 and HUVEC), viability was assessed byMTT assay and in terms of morphological features. Acridine orange (AO) and propidium iodide (PI) assays were carriedout to estimate the incidence of apoptosis. The RT-PCR method was also employed to evaluate the expression of P53,Bax, Bcl-2 and Caspase 3. Results: Exposure to increasing concentrations of TiO2 NPs enhanced overall cell survivalof HCT116 cells and reduced the Bcl-2 and Caspase 3 expression while the ratio of Bax/Bcl-2 was down-regulated.TiO2 NPs at 400 and 50 μg/ml concentrations suppressed cell proliferation and induced apoptosis of HT29 cells andalso up-regulated P53 and Bax at the mRNA level, enhanced the Bax/Bcl-2 ratio and eventually up-regulated Caspase3 mRNA. Although, inhibition of cell proliferation in HUVECs was seen at 200 and 400 μg/ml TiO2 NPs, it was notmarked. Conclusion: TiO2 NPs have selective bio-effects on exposed cells with dose- and cell-dependent influence onviability. Cell proliferation in HCT116 as a metastatic colorectal cancer cell line appeared to be stimulated via multiplesignaling pathways, with promotion of apoptosis in less metastatic cells at 50 and 400 μg/ml concentrations. This wasassociated with elevated P53, Bax and Caspase 3 mRNA and reduced Bcl-2 expression. However, TiO2 NPs did notexert any apparent significant effects on HUVECs as hyperproliferative angiogenic cells.
https://journal.waocp.org/article_69070_b146c89bf8ae70c63fa8ad8e224dd769.pdf
Colorectal cancer cells
HUVEC
TiO2 NPs
Cytotoxicity
Apoptosis
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2831
2838
10.22034/APJCP.2018.19.10.2831
69802
Posttraumatic Growth in Patients with Malignant Bone Tumor: Relationships with Psychological Adjustment
Ling Wang
wangling5333@csu.edu.cn
1
Siyao Chen
125144538@qq.com
2
Ping Liu
923287609@qq.com
3
Chun Zhu
252691751@qq.com
4
Muli Hu
humuli001@csu.edu.cn
5
Yanqian Li
siyaosiyao@126.com
6
Yan Tao
lusent6088@gmail.com
7
Zhe Huang
lusent126@126.com
8
Yi Zhou
278726436@qq.com
9
Tao Xiao
xiaotaoxyl@csu.edu.cn
10
Xiongzhao Zhu
xiongzhaozhu@163.com
11
Department of Orthopaedics, The Second Xiangya Hospital of Central South University, Changsha, China.
Department of Orthopaedics, The Second Xiangya Hospital of Central South University, Changsha, China.
Department of Orthopaedics, The Second Xiangya Hospital of Central South University, Changsha, China.
Department of Pheumology, The Second Xiangya Hospital of Central South University, Changsha, China.
Research Department, The Second Xiangya Hospital of Central South University, Changsha, China.
Hu Cancer Hospital, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, China.
Department of Orthopaedics, Xiangya Hospital of Central South University, Changsha, China.
Department of Orthopaedics, The Third Xiangya Hospital of Central South University, Changsha, China.
Department of Orthopaedics, Hunan Provincial People’s Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha, China.
Department of Orthopaedics, The Second Xiangya Hospital of Central South University, Changsha, China.
The Second Xiangya Hospital of Central South University, Changsha, China.
Aim: Owing to the inadequate data to support the valid instrument for assessing the positive changes among patientswith malignant bone tumor, the present study was designed to provide such valid evidence through examining thepsychometric properties of a Chinese version of the Posttraumatic Growth Inventory (PTGI-C) among these patients,and to evaluate the effects of posttraumatic growth on positive and negative symptoms in malignant bone tumor patients.Methods: Potential patients with malignant bone tumor from five tertiary hospitals were admitted to the hospital duringthe period from January 2013 to October 2017. At the baseline assessment (T1), all patients completed a demographicform, PTGI-C, and Positive and Negative Affect Scale (PANAS). After 4 weeks later (T2), all the patients finishedPANAS and PTGI-C again, and the PTGI-C was re-administered to patients who were simple randomly selected fromthe total sample. Results: The PTGI-C exhibited moderate reliability and validity. The Cronbach’s α coefficient of thetotal scale was 0.91. Confirmatory factor analysis supported the five-factor model and the convergent validity resultsobtained were distinct but correlated. The multiple linear regression analyses showed that posttraumatic growth hada significant prediction on positive affect (F=16.445, p<0.001), accounting for 69.4 % of the variance and as well asthe negative affect (F=8.707, p<0.001), accounting for 48.3 % of the variance. Personal strength at T1 was positivelyassociated with positive affect at T2, and more personal strength and spiritual change at T1 were associated with lessnegative affect at T2. Conclusions: PTGI-C has demonstrated sufficient psychometric properties which indicate thatit is appropriate to measure posttraumatic growth in patients with malignant bone tumor. Relating to others, spiritualgrowth, personal strength and appreciate to life are important factors which contribute to predicting positive affectand negative affect.
https://journal.waocp.org/article_69802_f5a2a3ba984eefc2633e6be4a430d5a2.pdf
Posttraumatic growth
Positive affect
Negative affect
Chinese patients
Malignant bone tumor
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2839
2844
10.22034/APJCP.2018.19.10.2839
70492
Effects of Grape Juice in Superoxide Dismutase and Catalase in Colorectal Cancer Carcinogenesis Induced by Azoxymethane
Carla Caroline Dias Ribeiro
carla.caroldi@gmail.com
1
Roseane Mendes Silva
rose_mendessilva@hotmail.com
2
Vanessa Maria de Lima Pazine Campanholo
vanessapazine@gmail.com
3
Daniel Araki Ribeiro
daribeiro@unifesp.br
4
Ana Paula Ribeiro Paiotti
a.paiotti@unifesp.br
5
Nora Manoukian Forones
noraforones@gmail.com
6
Department of Medicine – Discipline of Gastroenterology – Universidade Federal de São Paulo – Escola Paulista de Medicina, UNIFESP/EPM, SP, Brazil.
Department of Medicine – Discipline of Gastroenterology – Universidade Federal de São Paulo – Escola Paulista de Medicina, UNIFESP/EPM, SP, Brazil.
Department of Medicine – Discipline of Gastroenterology – Universidade Federal de São Paulo – Escola Paulista de Medicina, UNIFESP/EPM, SP, Brazil.
Department of Medicine – Discipline of Gastroenterology – Universidade Federal de São Paulo – Escola Paulista de Medicina, UNIFESP/EPM, SP, Brazil.
Department of Medicine – Discipline of Gastroenterology – Universidade Federal de São Paulo – Escola Paulista de Medicina, UNIFESP/EPM, SP, Brazil.
Department of Medicine – Discipline of Gastroenterology – Universidade Federal de São Paulo – Escola Paulista de Medicina, UNIFESP/EPM, SP, Brazil.
Background: The intestinal mucosa is commonly exposed to oxidant nutrients and carcinogens, which can lead tothe generation of free radicals. The antioxidants present in the diet assume great importance as possible protectiveagents, reducing the oxidative damage. In this way, we evaluated the antioxidant action of grape juice on preneoplasticlesions induced by azoxymethane (AOM) in Wistar rats. Methods: The colorectal carcinogenesis was induced by twointraperitoneal injections of 15mg/kg of AOM in Wistar rats. The animals were divided in 7 groups and treated with1 and 2% concentrations of grape juice before and after carcinogen administration. After euthanasia, the expression ofantioxidant enzymes catalase (CAT), copper-zinc superoxide dismutase (Cu/Zn-SOD) and manganese superoxidedismutase (Mn-SOD) CAT, SOD1 and SOD2 were evaluated by immunohistochemistry. Results: AOM decreased theexpression of CAT and Mn-SOD enzymes, but not for Cu/Zn-SOD. We observed an increase expression of CAT andMn-SOD after grape juice administration in some concentrations according to the time of administration of the grapejuice before the carcinogen or just after the carcinogen. Conclusion: Our results suggest an independent action of eachenzyme and a possible antioxidant action of the grape juice components in the diet being able to balance the body toneutralize the superoxide radicals and not leave them in the cell-damaging form.
https://journal.waocp.org/article_70492_2cb01edce2cd983637982c97b8394c8c.pdf
colorectal cancer
aberrant crypt foci
grape
antioxidants
free radicals
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2845
2851
10.22034/APJCP.2018.19.10.2845
69805
Dermal Lesions and Skin Cancer in Patients with Inflammatory Bowel Disease Receiving Immunosuppressive Therapy
Eftychia Giagkou
efigiagkou@yahoo.gr
1
Maria Saridi
sarmar32@windowslive.com
2
Eleni Albani
lenaalmpa@yahoo.gr
3
Georgios Gaitanis
ggaitan@cc.uoi.gr
4
Andreas Katsanos
andreas.katsanos@hotmail.com
5
Aris Bechlioulis
md02798@yahoo.gr
6
Athanasios-Dimitrios Bacasis
th.bacasis@icloud.com
7
Dimitrios K Christodoulou
dchristodoulou@gmail.com
8
Konstantinos H Katsanos
khkostas@hotmail.com
9
Department of Cardiology, University Hospital of Ioannina, Greece.
University of Peloponnese, Corinth, Greece.
Department of Nursing, TEI of Western Greece, Greece.
Department of Dermatology, Medical School of Ioannina, Greece.
Department of Ophthalmology, Medical School of Ioannina, Greece.
2nd Department of Cardiology University of Ioannina, Greece.
Department of Gastroenterology, Medical School of Ioannina, Greece.
Department of Gastroenterology, Medical School of Ioannina, Greece.
Department of Gastroenterology, Medical School of Ioannina, Greece.
Background: Anti-TNFa medications represent the first effective biologic therapy for IBD that has largelyrevolutionized treatment. The aim of this study was to quantify the risk of MM and other skin cancers among patientsfrom Northern Greece area with IBD who take immunosuppressive or biologic anti-TNF medications. Methods:The current study was conducted during a 3-year period (2014-2016). Clinical history and metabolic data of all patientswere extracted from the IBD database that is kept since 1980. 101 patients with IBD from Northwestern Greece,were studied. Results: The mean age of enrolled patients was 44.2±15.9 years old ranging from 17 years to 77 yearsold. No sun burn was reported from the 44.6% of the patients, 53.5% presented mild reticular veins in the face, andlack of any elastosis was noticed in 60.4%. The occurrence of two cases with squamous and basal cell carcinoma isan important finding. The absence of any case with MM should not quiet down but should strengthen our efforts forfurther implementation of preventive measures. Conclusions: Furthermore, education of patients to avoid deleterioussun exposure may help decrease MM incidence.
https://journal.waocp.org/article_69805_135549d721f54562ff3f34bd92cb6ea8.pdf
Skin Cancer
IBD
Anti-TNFa
Malignant Melanoma
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2853
2858
10.22034/APJCP.2018.19.10.2853
69072
GSTM1 and GSTT1 Polymorphisms and Susceptibility to Prostate Cancer: A Case-Control Study of the Algerian Population
Maroua Benabdelkrim
maroua_benabdelkrim@yahoo.fr
1
Omar Djeffal
omar_djeffal@yaho.fr
2
Hajira Berredjem
hajira.berredjem@univ-annaba.dz
3
Laboratory of Applied Biochemistry and Microbiology, Department of Biochemistry, Faculty of Sciences, University of Badji Mokhtar, Algeria.
Private Medical Cabinet of Uro-Surgery, Annaba, Algeria.
Laboratory of Applied Biochemistry and Microbiology, Department of Biochemistry, Faculty of Sciences, University of Badji Mokhtar, Algeria.
Objective: Prostate cancer (PCa) is a major public health problem worldwide, with high morbidity and mortality levels. Advanced age, androgen stimulation, and ethnicity have been reported to be possible risk factors. It has been suggested that particular genetic polymorphisms in glutathione S-transferases (GST), xenobiotic-metabolising enzymes, could predispose to prostate cancer through heritable deficiency in detoxification of environmental carcinogens. Conflicts in the published results and the absence of similar in depth studies in Algeria prompted us to perform the present case-control study of GSTM1 and GSTT1 polymorphisms and their possible association with PCa in an Algerian population. Methods: We determined GSTM1 and GSTT1 genotypes for 49 histologically verified prostate cancer patients and in 41 age-matched healthy controls by multiplex polymerase chain reaction (PCR) using peripheral blood DNA samples. Result: While an association between the GSTM1 null genotype and PCa risk (OR= 3.69, 95% CI= 1.30-10.44; P = 0.01) was evident, the GSTT1 null genotype (OR= 0.92, 95% IC= 0.32-2.62; P = 0.49) appeared without influence. Furthermore, no statistically significant differences between the double null genotype and PCa is detected, also no statistically significant differences between smoking status and PCa is detected. Conclusion: The GSTM1 null genotype may increase individual susceptibility to prostate cancer. On the other hand, the null-activity genotype of GSTT1 did not appear to contribute to the risk of prostate cancer in our population.
https://journal.waocp.org/article_69072_82cfcd66a67cf8e8f86a7bf6b928f376.pdf
Prostate Cancer
GSTM1
GSTT1
Genetic polymorphism
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2859
2866
10.22034/APJCP.2018.19.10.2859
69903
PAX6 Promoter Methylation Correlates with MDA-MB-231 Cell Migration, and Expression of MMP2 and MMP9
Guillermo Urrutia
gurrutia@mcw.edu
1
Sergio Laurito
slaurito@mendoza-conicet.gob.ar
2
Emanuel Campoy
ecampoy@mendoza-conicet.gob.ar
3
Daniela Nasif
nasifdaniela@gmail.com
4
María Teresita Branham
mbranham@mendoza-conicet.gob.ar
5
María Roqué
mroque@mendoza-conicet.gob.ar
6
IHEM, National University of Cuyo, CONICET, Mendoza, Argentina.
IHEM, National University of Cuyo, CONICET, Faculty of Exact and Natural Sciences, Mendoza, Argentina.
IHEM, National University of Cuyo, CONICET, Faculty of Medical Sciences, Mendoza, Argentina.
IHEM, National University of Cuyo, CONICET, Mendoza, Argentina.
IHEM, National University of Cuyo, CONICET, Mendoza, Argentina.
IHEM, National University of Cuyo, CONICET, Faculty of Exact and Natural Sciences, Mendoza, Argentina.
Objective: Breast cancer is a heterogeneous disease characterized by an accumulation of genetic and epigeneticalterations that lead tumor cells to acquire characteristics like the capacity for invasion and metastasis. Metastasisremains a major challenge in cancer management and understanding of its molecular basis should result in improvedprevention, diagnosis, and treatment of breast cancer patients. The aim of this study was to investigate how promoterDNA methylation regulates PAX6 gene expression and influences breast carcinoma cell migration. Methods: PAX6promoter methylation was detected by Methyl Specific-Multiplex Ligation Probe Amplification (MS-MLPA). Geneexpression was evaluated using qRT-PCR, while the effect of PAX6 on migration was ssessed by wound healing assay.In addition, MMP2 and MMP9 genes were studied using different bioinformatic tools. Results: The PAX6 promoter ismethylated in breast cancer cell lines and methylation in this region impacts on its expression. Migration assays revealedthat PAX6 overexpression promotes cell migration, while PAX6 inhibition decreases it. More importantly, we foundthat migration is affected by PAX6 methylation status. Employing bioinformatic analysis, binding sites for PAX6 onthe regulatory regions of the MMP2 and MMP9 genes were established, PAX6 overexpression increasing MMP2 andMMP9 expression at the mRNA level. Conclusion: Our study provides novel insights into epigenetic events that regulatePAX6 expression and molecular mechanisms by which PAX6 modifies the migration capacity of breast cancer cells.
https://journal.waocp.org/article_69903_1459356ca0bf062e32e822d1a247d04c.pdf
breast cancer
Metastasis
DNA Methylation
PAX6
Matrix Metalloproteinases
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2867
2870
10.22034/APJCP.2018.19.10.2867
69121
The Inflammatory Prognostic Index Predicts Cancer-Specific Outcomes of Patients with Resected Non-Small Cell Lung Cancer
Masaki Tomita
mtomita@med.miyazaki-u.ac.jp
1
Takanori Ayabe
2
Ryo Maeda
3
Kunihide Nakamura
4
Department of Thoracic and Breast Surgery, Faculty of Medicine, University of Miyazaki, Kihara 5200, Kiyotake, Miyazaki, 889-1692, Japan.
Department of Thoracic and Breast Surgery, Faculty of Medicine, University of Miyazaki, Kihara 5200, Kiyotake, Miyazaki, 889-1692, Japan.
Department of Thoracic and Breast Surgery, Faculty of Medicine, University of Miyazaki, Kihara 5200, Kiyotake, Miyazaki, 889-1692, Japan.
Department of Cardiovascular Surgery, Faculty of Medicine, University of Miyazaki, Kihara 5200, Kiyotake, Miyazaki, 889-1692, Japan.
Background: Previous study developed a new inflammatory prognostic index (IPI) and found the prognostic valueof IPI for all stage non-small cell lung cancer (NSCLC). To the best of our knowledge, however, no studies regardingIPI in patients with resected NSCLC are available. Methods: Three hundred forty-one NSCLC patients who underwentsurgery at our institution were included. The IPI was calculated as C-reactive protein × neutrophil-to-lymphocyte ratio(NLR)/serum albumin. The optimal cut-off value was calculated by the Cutoff Finder. Univariate and multivariateanalyses were calculated by the Cox proportional hazards regression model. Results: The optimal cut-off value was5.237 for IPI. The IPI was associated with age, gender, smoking status, histology, pT status and serum CYFRA21-1level, but not pStage, pN status and serum carcinoembryonic antigen level. The 5-year cancer-specific survival ofpatients with low IPI was significantly better than that with high IPI (84.8% vs. 57.9%, p< 0.001). Furthermore, low IPIwas significantly associated with favorable cancer-specific survival in univariate (HR =0.326, 95% CI =0.212-0.494;p<0.001) and multivariate (HR =0.438, 95% CI =0.276-0.690; p=0.001) analyses. Conclusion: This is the first studyto demonstrate that IPI might serve as an efficient prognostic indicator in resected NSCLC.
https://journal.waocp.org/article_69121_142bff32b1d4dc79716dd829a3d55b79.pdf
inflammatory prognostic index
non-small cell lung cancer
Surgery
cancer-specific survival
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2871
2876
10.22034/APJCP.2018.19.10.2871
70493
Spatio-Temporal Study of Gastric Cancer Incidence in Kermanshah Province, Iran During the Years 2009-2014
Abozar Solimany
abozarsolimany@yahoo.com
1
Malihe Khoramdad
m.khoramdad2016@gmail.com
2
Nahid Khademi
nahidkhademi2003@yahoo.com
3
Ali Delpisheh
alidelpisheh@yahoo.com
4
Faculty of Health, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Student Research Committee, Faculty of Public Health Branch, Iran University of Medical Sciences, Tehran, Iran.
Faculty of Health, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Department of Epidemiology, Ilam University of Medical Sciences, Ilam, Iran.
Background and objective: Stomach cancer is the second common and the most deadly type of cancer in Iranianpopulations. The pattern of this cancer varies in different populations; demonstrating association with environmental,racial, and geographical factors. The focus of this study was to identify the clustering and the high-risk and low-riskregions for stomach cancer by using spatio-temporal analysis in Kermanshah province during 2009-2014. Method:All new cases of stomach cancer were studied by census method in 2009 to end 2014 in Kermanshah province.The required information and statistics (address, age, and sex) of patients were extracted from the cancer registrysystem of Vice-Chancellor in health affairs, Kermanshah University of Medical Sciences. Also, with havingthe location, diagnosis time, and counting information of all age groups of stomach cancer patients, the spatiallyand temporally abnormal places of clustering were identified with the use of saTScan and GIS. Results: the totalnumber of stomach cancer subjects during the period of study was 1040, with a mean age of 66.5± 1. in addition, 11regions (located in 1st, 2nd, and 3rd municipal districts) demonstrated abnormal Spatio-temporal pattern of stomachcancer incidence (hot spots) and clustered disease, with 5 regions (in 4th and 5th districts) representing lower chance ofclustering (cold spots, p value˂0.05). Conclusion: Given the growing rate of stomach cancer incidence in specificgeographical areas and, its high potential of mortality, and the possible relationship with environmental variables(e.g. climate variables); the efforts need to be focused on the identification of hot/cold spots, the predisposing factors,and the possible clusters in the affected areas.
https://journal.waocp.org/article_70493_8783bf32bae4a6d14bdcc21386baab87.pdf
Gastric cancer
disease clustering
hot spots
cold spots
Spatio-temporal analysis
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2877
2884
10.22034/APJCP.2018.19.10.2877
69904
Effect of a Copper (II) Complex on The Induction of Apoptosis in Human Hepatocellular Carcinoma Cells
Azadeh Rezaei
a.rezaee2012a@yahoo.com
1
Soudeh Khanamani Falahati-pour
s.falahatipour@rums.ac.ir
2
Fatemeh Mohammadizadeh
fateme.mzh@gmail.com
3
Mohammad Reza Hajizadeh
hajizadehus@yahoo.com
4
Mohammad Reza Mirzaei
mirzaeemr@gmail.com
5
Alireza Khoshdel
alireza.khoshdel@gmail.com
6
Mohammad Ali Fahmidehkar
fahmidehkarma@gmail.com
7
Mehdi Mahmoodi
mahmoodi1338@gmail.com
8
Department of Clinical Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
Pistachio Safety Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
Department of Clinical Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
Department of Clinical Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
Department of Clinical Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
Department of Clinical Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
Research Center of Advanced Technologies in Medicine, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran.
Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
Objectives: In the present study, we aimed to identify the anti-proliferative potential of [Cu(L)(2imi)] complex[L = 2-(((5-chloro-2-oxyphenyl)imino)methyl)phenolato) and 2imi = 2-methyl imidazole] against HepG2 cells as anin vitro model of human hepatocellular carcinoma and normal mouse fibroblast L929 cells. Methods: The cytotoxicand apoptotic effects of [Cu(L)(2imi)] complex on HepG2 cells and normal fibroblasts (L929) were examined by MTTassay and flow cytometry, respectively. Results: Cytotoxicity induced by [Cu(L)(2imi)] complex was time dependent.Also, there was a positive correlation between cytotoxicity and an increase in Cu complex concentration. For HepG2cells, the cell viability percentage was 50% at 58 μg/mL after 24 h treatment, whereas in the same concentration andconditions, the viability percentage was surprisingly higher (about 100%) for L929 cells. Also, after 48 h treatment,the viability percentage of HepG2 cells at 55 μg/mL concentration was 50% in contrast with 89.3% for L929 cells inthe same conditions. Flow cytometry findings suggest that [Cu(L)(2imi)] complex is capable of decreasing cancer cellviability through apoptosis and did not efficiently activate the necrosis process. Conclusions: Finally, we found that[Cu(L)(2imi)] complex possess the potential for development as an anti-cancer drug for human hepatocellular carcinoma.
https://journal.waocp.org/article_69904_531a2d8bddc07a4bf128db39d3160c10.pdf
Apoptosis
[Cu(L)(2imi)] complex
Cytotoxicity
Hepatocellular carcinoma
Mouse fibroblast L929 cells
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2885
2889
10.22034/APJCP.2018.19.10.2885
69804
Population Based Cancer Registry of India – the Challenges and Opportunities
Priyamadhaba Behera
priya.madhaba@gmail.com
1
Binod Kumar Patro
patrobinod@aiimsbhubaneswar.edu.in
2
Department of Community and Family Medicine, All India Institute of Medical Sciences, AIIMS, Bhubaneswar, Odisha, India.
Department of Community and Family Medicine, All India Institute of Medical Sciences, AIIMS, Bhubaneswar, Odisha, India.
Cancer is one of the important causes of morbidity and mortality in India. Globally, out of 14 million diagnosednew cancer cases slightly more than 1 million were from India. Population Based Cancer Registries (PBCRs) playsa vital role in formulating cancer control plans as well as in monitoring their success. The article identifies challengesand opportunities for the PBCRs in India. Major challenges of PBCRs in India are-low coverage, urban dominance,quality assurance in data, less awareness among rural people, lack of follow-up and survival data, timeliness, highcost of registration, non-linkage of PBCR with other PBCRs and Hospital Based Cancer Registries (HBCRs) andgeneralization of estimates at country level. Expansion of cancer atlas project, real time data collection, enteringAadhar number (UID) during registration and establishing linkage among various PBCRs and PBCRs with HBCRswill improve cancer registration and its quality on a short-term basis. However, the opportunity of development newPBCRs in linkage with existing Health and Demographic Surveillance System (HDSS) will increase coverage as wellas sustainability of PBCRs in India.
https://journal.waocp.org/article_69804_dcce90833c4a8ca028f564f0834b3499.pdf
cancer
Population-Based Cancer Registries (PBCRs)
India
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2891
2895
10.22034/APJCP.2018.19.10.2891
69129
The Role of Single Voxel MR Spectroscopy, T2 Relaxation Time and Apparent Diffusion Coefficient in Determining the Cellularity of Brain Tumors by MATLAB Software
Jamil Abdolmohammadi
abdolmohammadi.jamil@gmail.com
1
Fariborz Faeghi
f_faeghi@sbmu.ac.ir
2
Dooman Arefan
d.arefan139@yahoo.com
3
Alireza Zali
dr_a_zali@yahoo.com
4
Hamidreza Haghighatkhah
drhrhaghighatkhah@yahoo.com
5
Jamal Amiri
amirijamal123@gmail.com
6
Department of Radiology, Faculty of Paramedical Sciences, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Department of Radiology Technology, School of Allied Medical Sciences, Shahid Beheshti University of Medical Science, Tehran, Iran.
Department of Radiation Medicine Engineering, Shahid Beheshti University, Evin, Tehran, Iran.
Department of Neurosurgery, Shohada-E Tajrish Hospital, Chairman of the Medical Council of Iran, Tehran, Iran.
Department of Radiology, Shohada-E Tajrish Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Radiotherapy, Faculty of Paramedical Sciences, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Introduction: Brain tumors if timely diagnosed are sure to be treated through shorter processes. MRI amongstothers is of Para clinical methods greatly effective in diagnosis phase. Diffusion-weighted imaging (DWI) and apparentdiffusion coefficient (ADC) maps provide some information that could reflect tissue cellularity. Neurosurgeons, inparticular to detect the tumor cellularity, must send the specimens taken through biopsy to the pathology unit. Thisstudy is aimed at determining the tumor cellularity in brain. Materials and Methods: In this cross-sectional study, 32patients (18 males and 14 females of the range 18 – 77 y/o) between April 2014 and February 2016 who were referredto the neurosurgery department of Shohada-E Tajrish Hospital of Tehran participated. Imaging was made using singlevoxel MR Spectroscopy, ADC and T2W Multi Echo Pulse Sequence in addition to routine pulse sequences and theimages were analyzed using MATLAB software to determine the cellularity of brain tumors in comparison to the biopsy.Results: findings showed that by decreasing T2 relaxation time, the amount of ADC, N-Acetyl Aspartate (NAA) andalso, increase Choline metabolite, lead to registering tumors in the lower class on the designed table and these tumorshave a higher degree of consistency and cellularity. T2 Relaxation time, the tumors will stand at higher class on thedesigned table. Also the results indicated that 85% diagnostic weight of T2 relaxation time and 83% diagnostic weightof ADC compared with biopsy could reveal the brain tumor cellularity (P>0.05). Conclusion: some cellular metabolitechanges such as NAA and Choline, ADC value and T2 relaxation time feature could effectively be used to distinguishand illustrate the degree of cellularity of brain tumors especially Intra-axial brain tumors (with about 85%. vs. biopsy).We recommend to more data should be used to increase the accuracy percentage of this technique.
https://journal.waocp.org/article_69129_1407f2a1692ae3def2fef1eeeb21ca80.pdf
MATLAB software
T2 relaxation time
Brain tumor
MRI
MRS
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2897
2903
10.22034/APJCP.2018.19.10.2897
69088
Meta-Analysis of Association between PALB2 Polymorphisms and Breast Cancer
Ali Dianatopur
ali.dianatpur@gmail.com
1
Sepideh Faramarzi
s.faramarzi2008@gmail.com
2
Soudeh Ghafouri-Fard
s.ghafourifard@sbmu.ac.ir
3
Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Background: Previous studies have assessed associations between single nucleotide polymorphisms (SNPs) ofthe Partner and localizer of BRCA2 (PALB2) gene and risk of breast cancer. However, the results of these studiesare not consistent. Materials and Methods: We designed a meta-analysis to obtain a more reliable appraisal ofthe association between SNPs in the PALB2 gene and the susceptibility to breast cancer. We searched PubMed, Googlescholar and Embase databases and selected six studies with sufficient data to estimate the pooled odds ratios (ORs)and 95% confidence intervals (CIs). Results: Statistical analyses showed that the rs120963 was associated with breastcancer risk in allelic (OR (95% CI) = 1.33 (1.18-1.49)), homozygous (OR (95% CI) = 1.74 (1.31-2.32)), dominant(OR (95% CI) = 1.42 (1.22, 1.65)) and recessive (OR (95% CI) = 1.54 (1.17, 2.03)) models. The rs249954 andrs16940342 were associated with breast cancer risk in allelic (OR (95% CI) = 1.13 (1.04, 1.23) and 1.12 (1.01, 1.24)respectively) and dominant (OR (95% CI) = 1.23 (1.09, 1.39) and 1.18 (1.04, 1.33) respectively) models. The rs249935and rs447529 SNPs were associated with breast cancer in homozygous (OR (95% CI) = 0.67 (0.46, 0.97) and 0.51(0.30, 0.89) respectively) and recessive (OR (95% CI) = 0.65 (0.45, 0.95) and 0.51 (0.30, 0.88) respectively) models.Conclusions: The current meta-analysis shows the associations between five SNPs of PALB2 and breast cancer riskand confirms the results of previous studies regarding the role of this gene in the pathogenesis of breast cancer.
https://journal.waocp.org/article_69088_5d88c9c8106a2dded5be54616844f5a3.pdf
PALB2
breast cancer
Meta-analysis
Polymorphism
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2905
2910
10.22034/APJCP.2018.19.10.2905
69131
Spectrum of the KIT Gene Mutations in Gastrointestinal Stromal Tumors in Arab Patients
Muhammad Faiyaz-Ul-Haque
mhaque2@hamad.qa
1
Fouad Al-Dayel
dayelf@kfshrc.edu.sa
2
Asma Tulba
tulbah@kfshrc.edu.sa
3
Halah Abalkhail
habaalkhail@kfshrc.edu.sa
4
Hussa Alhussaini
hfalhussaini@hotmail.com
5
Muhammad Memon
memon@kfshrc.edu.sa
6
Shouki Bazarbashi
bazarbashi@kfshrc.edu.sa
7
Tarek Amin
tamin@kfshrc.edu.sa
8
Mohamed B Satti
sattimb@ngha.med.sa
9
Iskra Peltekova
ipeltekova@hollandbloorview.ca
10
Zafar Nawaz
znawaz@hamad.qa
11
Syed H E Zaidi
hassan.zaidi@utoronto.ca
12
Department of Pathology, College of Medicine, King Saud University, Riyadh, Saudi Arabia.
Department of Pathology and Laboratory Medicine, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
Department of Pathology and Laboratory Medicine, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
Department of Pathology and Laboratory Medicine, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
Department of Pathology and Laboratory Medicine, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
King Faisal Cancer Centre, King Faisal Specialist Hospital & Research Centre, Riyadh, Saudi Arabia.
King Faisal Cancer Centre, King Faisal Specialist Hospital & Research Centre, Riyadh, Saudi Arabia.
King Faisal Cancer Centre, King Faisal Specialist Hospital & Research Centre, Riyadh, Saudi Arabia.
Department of Pathology, King Abdulaziz Medical City, Jeddah, Saudi Arabia.
Department of Pediatrics, University of Toronto, Holland Bloorview Kids Rehabilitation Hospital, Toronto, Canada.
Diagnostic Genomic Division, Department of Laboratory Medicine and Pathology, Hamad Medical Corporation, Doha, Qatar.
Genomics, Ontario Institute for Cancer Research, Toronto, Canada.
Background: Gastrointestinal stromal tumors are the most common mesenchymal tumors of the gastrointestinaltract, which originate from the interstitial cells of Cajal. These tumors are characterized by expression of CD117 andCD34 antigens and activating mutations in the KIT and PDGFRA genes. While KIT and PDGFRA mutations have beenextensively studied in other populations, the spectrum of mutations in Arab patients remains unknown. The study aimedat determining the distribution of KIT and PDGFRA mutations and phenotypic characterization of the gastrointestinalstromal tumors in Arab patients. Methods: Sanger sequencing was used to analyze 52 archived gastrointestinal stromaltumors for mutations in the KIT and the PDGFRA genes. Tumor descriptions were obtained from the clinical reportsof patients. Results: In these patients, most tumors occur in the stomach, followed by the rest of the digestive tract. Avast majority of tumors express the CD117 and CD34 antigens. Sequencing of the KIT and PDGFRA genes identifiedfive non-synonymous mutations and 26 deletions (25 novel) in exon 11 of the KIT gene. All non-synonymous mutationsand deletions affect the juxta-membrane domain, which is known to inhibit ligand-independent activation of the KITreceptor. No mutations were found in the PDGFRA gene. Conclusions: Molecular profiling of the gastrointestinalstromal tumors in Arab patients identified a unique spectrum of mutations in exon 11 of the KIT gene. These data areimportant for the diagnosis and management of patients of Arab ethnic origin.
https://journal.waocp.org/article_69131_87f1bc39d4463b82aee5eb9ca9053adc.pdf
Gastrointestinal stromal tumors
KIT mutations
Arab patients
CD117
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2911
2916
10.22034/APJCP.2018.19.10.2911
70494
Apoptotic Effects of Linum album Extracts on AGS Human Gastric Adenocarcinoma Cells and ZNF703 Oncogene Expression
Elham Akbari Asl
elhamakbari_66@yahoo.com
1
Jalil Fallah Mehrabadi
jalil.fallah@gmail.com
2
Davood Afshar
davoodafshar@yahoo.com
3
Hassan Noorbazargan
h.noorbazargan@gmail.com
4
Hossein Tahmasebi
milad_tahmasbi_h@yahoo.com
5
Arian Rahimi
arianrahimi1988@gmail.com
6
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
The Lister Laboratory of Microbiology, Tehran, Iran.
Department of Microbiology and Virology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Cellular and Molecular Biology, Ahar Branch, Islamic Azad University, Tabriz, Iran.
Young Researchers and Elite Club, East Tehran Branch, Islamic Azad University, Tehran, Iran.
Introduction: Linum album is a medicinal plant endemic in Iran that is very important pharmaceutically. The presentstudy concerns the effect of different extracts of L. album on ZNF703 gene expression and apoptosis in human gastriccarcinoma AGS cells. Method and material: Hydro alchoholic L. album extracts from various plant sources wereproduced by Maceration. AGS cells were treated with different concentrations (200, 400, 600, 800 and 1000 μg/ml)and the cytotoxicity potency was assessed after 24 h by MTT assay. Then, quantitative real time PCR was conductedfor ZNF703 gene expression in AGS cells. Also, cell apoptosis/necrosis was assessed with the aid of Annexin V/PIstaining and quantification by flow cytometry. Results: L. album extracts exerted dose-dependent toxicity in the AGScell line. The mRNA levels of ZNF703 gene expression were significantly decreased with rhizome, fruit at fruiting,leaf and stem at anthesis (P<0.001), and leaf and stem at fruiting extracts as compared to the controls (P<0.01). Also,the number of apoptotic cells was increased from 2.70% (statistically significant; p<0.05) in untreated AGS cells to 44%,following treatment with the leaf and stem at anthesis example. Discussion: Our findings revealed that the L. albumextracts can induce apoptosis and might modulate cytotoxicity by down regulating ZNF703 gene expression in AGScells. Therefore, this extract could be a good candidate for inhibiting cancer cell growth, especially that of gastriccancer. In addition, ZNF703 may have potential as a therapeutic target.
https://journal.waocp.org/article_70494_d7e97ebed63a39fffd488f7145872e2c.pdf
Linum album
Gastric cancer
ZNF703
Apoptosis
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2917
2920
10.22034/APJCP.2018.19.10.2917
69123
Comparison of Bayes Classifiers for Breast Cancer Classification
Bazila Banu A
bazilabanu@bitsathy.ac.in
1
Ponniah Thirumalaikolundusubramanian
ponniah.tks@gmail.com
2
Department of Computer Science and Engineering, Bannari Amman Institute of Technology, Sathyamangalam, India.
Department of Medicine, Professor, Chennai Medical College Hospital and Research Centre, Irungatur, Trichy, Tamilnadu, India.
Data analytics play vital roles in diagnosis and treatment in the health care sector. To enable practitioner decisionmaking,huge volumes of data should be processed with machine learning techniques to produce tools for predictionand classification. Diseases like breast cancer can be classified based on the nature of the tumor. Finding an effectivealgorithm for classification should help resolve the challenges present in analyzing large volume of data. The objectivewith this paper was to present a report on the performance of Bayes classifiers like Tree Augmented Naive Bayes(TAN), Boosted Augmented Naive Bayes (BAN) and Bayes Belief Network (BBN). Among the three approaches, TANproduced the best performance regarding classification and accuracy. The results obtained provide clear evidence forbenefits of TAN usage in breast cancer classification. Applications of various machine learning algorithms could clearlyassist breast cancer control efforts for identification, prediction, prevention and health care planning.
https://journal.waocp.org/article_69123_998d1e424189e63fbb8a29240cb03ebd.pdf
Tree Augmented Naive Bayes (TAN)
Boosted Augmented Naive Bayes (BAN)
Bayes Network (BN)
Gradient Boosting (GB)
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2921
2927
10.22034/APJCP.2018.19.10.2921
69076
Glutathione S-transferase M1 (GSTM1) and T1 (GSTT1) Polymorphisms and Lung Cancer Risk among a Select Group of Iranian People
Glavizh Adibhesami
glavizh.adib@gmail.com
1
Gholam Reza Shahsavari
reza13sh@gmail.com
2
Ali Amiri
ali_pul_amiri@yahoo.com
3
Amir Nader Emami Razavi
razavinader@gmail.com
4
Masoud Shamaei
dr_shamaei@yahoo.com
5
Mehdi Birjandi
mehdibirjandi@yahoo.com
6
Department of Biochemistry and Genetics, Lorestan University of Medical Sciences, Khorramabad, Iran.
Department of Biochemistry and Genetics, Lorestan University of Medical Sciences, Khorramabad, Iran.
Pulmonary Department, Lorestan University of Medical Science, Khorramabad, Iran.
Iran National Tumor Bank, Cancer Biology Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran.
Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Nutritional Health Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
Objective(s): Lung cancer, caused primarily by smoking, is one of the leading determinants of mortality throughoutthe world. Here we investigated the effects of polymorphisms in two enzymes, i.e., GSTT1 and GSTM1, related tothe antioxidant defense line against carcinogens associated with lung cancer among a select group of Iranian people.Materials and Methods: One hundred and twenty lung cancer patients from two referral centers in Tehran, Iran, wererecruited for comparison with 120 healthy controls. Genomic DNA was extracted from the FFPE tumor tissues ofthe select cases and peripheral blood buffy coats of healthy controls. The polymorphisms of GSTT1 and GSTM1 wereinvestigated by multiplex polymerase chain reaction. Results: With the 240 samples studied, no specific relationshipwith lung cancer was discerned for the GSTM1 (P=0.35; OR=1/33; 95% CI=0.79-2.25) polymorphism, but the GSTT1(P=0.005; OR=2.4; CI=1.32-4.35) gene polymorphism revealed a notable association on logistic regression, takinginto account age and sex factors. Furthermore, the GSTT1 genotype distribution in patients with LSCC was differentfrom that of healthy cases (P=0.006; OR=3.11; CI=1.38-7.04). The risk of developing lung cancer with the T0M1genotype was 3.46 times higher than with T1M1 genotype (P=0.002; OR=3.46; CI=1.61-7.46). Moreover, the risk ofdeveloping LSCC cancer in people with T0M1 genotypes was significantly elevated (P=0.004; OR=4.5; CI=1.62-12.52).Conclusion: Unlike GSTM1, the GSTT1 genotype distribution is associated with the incidence of lung cancer in Iranianpeople. Different types of lung cancer appear to show various correlations with GST polymorphisms in this regard.
https://journal.waocp.org/article_69076_53a93b8add7562e23178872da0f37e6f.pdf
Glutathione S-transferases T1 (GSTT1)
Glutathione S-transferases M1 (GSTM1)
Lung cancer
Multiplex PCR
Polymorphism
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2929
2934
10.22034/APJCP.2018.19.10.2929
67431
Comparision of Different Radiotherapy Planning Techniques for Breast Cancer after Breast Conserving Surgery
Narudom Supakalin
oakblackbird@gmail.com
1
Montien Pesee
pmonti@kku.ac.th
2
Komsan Thamronganantasakul
komsth@kku.ac.th
3
Kiattisak Promsensa
4
Chunsri Supaadirek
5
Srichai Krusun
6
Division of Radiotherapy, Department of Radiology, Srinagarind Hospital, Faculty of Medicine, Khon Kaen University, Thailand.
Division of Radiotherapy, Department of Radiology, Srinagarind Hospital, Faculty of Medicine, Khon Kaen University, Thailand.
Division of Radiotherapy, Department of Radiology, Srinagarind Hospital, Faculty of Medicine, Khon Kaen University, Thailand.
Division of Radiotherapy, Department of Radiology, Srinagarind Hospital, Faculty of Medicine, Khon Kaen University, Thailand.
Division of Radiotherapy, Department of Radiology, Srinagarind Hospital, Faculty of Medicine, Khon Kaen University, Thailand.
Division of Radiotherapy, Department of Radiology, Srinagarind Hospital, Faculty of Medicine, Khon Kaen University, Thailand.
Objectives:To compare different radiotherapy planning techniques for breast cancer after breast conserving surgery.Materials and methods: Eighteen patients with breast cancer who underwent breast conserving surgery were selected.For each patient four different whole breast irradiation techniques including Tan, fIMRT, iIMRT and VMAT werecompared to the conventional tangential technique (Tan). Results: Mean maximum point dose (Dmax) for Tan, fIMRT,iIMRT and VMAT were 110.17% (±1.87), 105.89% (±1.13), 106.47% (±0.92) and 106.99% (±1.16) (p<0.001). Meanminimum point dose (Dmin) from Tan was 84.02% (±3.68) which was significantly higher than those from fIMRT,iIMRT and VMAT which were 76.57% (±11.4), 67.69 %( ±19.20) and 80.69% (±7.06) (p<0.001). Only the meanV95 of fIMRT was significantly less than Tan (p=0.01). Mean percentage of volume receiving ≥ 20 Gy (V20Gy) andmean doses of the ipsilateral lung were 17.09% and 953.05 cGy, 16.60% and 879.20 cGy, 14.79% and 772.26 cGy,15.32% and 984.34 cGy for Tan, fIMRT, iIMRT and VMAT. Only iIMRT had a significantly lower mean V20Gy andthe mean dose to ipsilateral lung in comparison with Tan. Significantly, high mean doses to the contralateral breast(498.07 cGy, p<0.001) were observed in VMAT. Conclusion: The conventional tangential technique provides adequatedose coverage but resulted in high dose-volumes. The iIMRT and fIMRT had significantly smaller high dose-volumesand better conformity. VMAT demonstrated excellent dose homogeneity and conformity but an increased low-dosevolume outside the target should be of concern.
https://journal.waocp.org/article_67431_2d91645c7c05cd95e256c4ccaf45c631.pdf
breast cancer
Radiotherapy planning technique
IMRT
VMAT
Conventional tangential technique
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2935
2941
10.22034/APJCP.2018.19.10.2935
69124
Health-Seeking Behaviour and Delayed Presentation of Oral Cancer Patients in a Developing Country: A Qualitative Study based on the Self-Regulatory Model
Nurizyani Azhar
izyani2orange@gmail.com
1
Jennifer Geraldine Doss
jendoss@um.edu.my
2
Department of Community Oral Health and Clinical Prevention, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia.
Oral Cancer Research, Coordinating Centre (OCRCC), Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia.
Background: The aim of this study was to explore reasons for delayed health-seeking for late stage oral cancerpatients. Methods: Semi-structured in-depth interviews were conducted with 35 oral cancer patients with TNM stageIII to IV disease, who were treated at six tertiary regional centres managing oral cancer throughout Malaysia. Interviewswere audio-recorded, transcribed verbatim, coded using NVivo (version 10.0) qualitative software and analysedusing framework analysis. Results: Participants interpreted their early symptoms as a minor condition and did notconsider it as requiring immediate attention. Four types of coping strategies causing delayed help-seeking emerged:1) self-remedy 2) self-medication 3) seeking traditional healers and 4) consulting general medical practitioners (GPs)instead of dentists. Socio-economic factors, cultural beliefs and religious practices have some influence on diagnosticdelay. Conclusion: Low levels of public knowledge and awareness regarding early signs and symptoms of oral canceras well as GPs’ misdiagnosis of early lesions results in delayed diagnosis.
https://journal.waocp.org/article_69124_f1650f64b36658b7bf984d74899cfd27.pdf
oral cancer
health-seeking behaviour
awareness
self-regulatory model
patient related-factors
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2943
2946
10.22034/APJCP.2018.19.10.2943
69125
Breast Cancer in Basra Oncology Center: A Clinico- Epidemiological Analysis
Rafid A Abood
rafidalkhalidy79@gmail.com
1
Basra College of Medicine, Basra, Iraq.
Background: Breast cancer is the most common cancer affecting women, and the leading cause of cancer-relateddeaths. Objective: This study was performed to evaluate clinico-epidemiological features of breast cancer from Iraqduring a five-year period. Methodology: This is a retrospective descriptive study. Medical notes and histopathologicalreports of patients with confirmed diagnosis of breast cancer between January 2011 and December 2015 were reviewedfor age, gender, site, laterality, histopathological type, grade of differentiation and TNM stage at diagnosis. Results:A total of 1,000 patients were included in the study. Mean age at diagnosis was 50 years (range 22-85 years), andfemales constituted 99.2% of cases. Most cases (98.7%) were unilateral and most common (85.5%) histological subtypewas invasive ductal carcinoma. Majority of the cases (58%) were moderately differentiated (grade II), wherein 45%belonged to stage II in TNM system, and nearly half (49%) of patients had locally advanced or metastatic cancer.Conclusion: Breast cancer presents at least a decade earlier and at a more advanced stage in Iraqi women whencompared to the Western World. Steps for early detection are essential for initiation of prompt therapy and reductionof mortality.
https://journal.waocp.org/article_69125_d589358a33ad0493917221f04abe5c5e.pdf
KEYWORDS: Breast cancer
TNM staging
NGS histological grading
Epidemiology
Iraq
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2947
2954
10.22034/APJCP.2018.19.10.2947
69126
A Spatial Survival Model in Presence of Competing Risks for Iranian Gastrointestinal Cancer Patients
Saeed Hesam
saeed_hesam65@yahoo.com
1
Mahmood Mahmoudi
mahmoodim@tums.ac.ir
2
Abbas Rahimi Foroushani
abbas_37@yahoo.com
3
Mehdi Yaseri
m.yaseri@gmail.com
4
Mohammad Ali Mansournia
mansournia_ma@yahoo.com
5
Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Background: Gastrointestinal cancer is one of the common causes of death from cancer in Iran. Survival analysisis usually used to detect prognostic factors of time to death from gastrointestinal cancers. The use of ordinary survivalmodels, in the presence of competing risks and/or when data is collected within geographic areas, may lead to distortingthe results. Therefore, the aim of this study is to use the spatial survival models in the presence of competing risks toassess the risk factors affecting the survival time of gastrointestinal cancer patients. Methods: The data in this studywas collected from 602 patients who were diagnosed with gastrointestinal cancer in Golestan and Mazandaran provincesregistered in Iran’s National Institute of Health Research from 2002 through 2007 and were followed up to July 2017.The data was analyzed using the cause-specific hazard frailty model with multivariate conditional autoregressivedistribution for frailties in the presence of competing risks (death from gastrointestinal cancer, heart disease, and othercauses) via OpenBUGS software. Results: The hazard of death from gastrointestinal cancer in men patients, patientswho lived in rural areas, patients whose relatives did not have a history of cancer, patients who did not undergo surgery,and patients with gastric cancer was significantly higher than others. Based on the deviance information criterion (DIC),frailty models and spatial frailty models seemed better than no-frailty model and non-spatial frailty model, respectively.Conclusions: This study showed that the use of the spatial frailty term in the model helps better fit the model. Also,the spatial pattern in the figures suggests the necessity of presence of some still missing, spatially varying covariatesrelevant for time to death from gastrointestinal cancer, heart disease, or other causes.
https://journal.waocp.org/article_69126_caa2dd9dd0392359c17fc0975e754be6.pdf
Gastrointestinal cancer
Survival Analysis
Competing risks
Spatial survival model
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2955
2962
10.22034/APJCP.2018.19.10.2955
69801
Synergistic Action of 1,2-Epoxy-3 (3- (3,4-dimethoxyphenyl)-4H-1-benzopiyran-4-on) Propane with Doxorubicin and Cisplatin through Increasing of p53, TIMP-3, and MicroRNA-34a in Cervical Cancer Cell Line (HeLa)
Lelly Yuniarti
lelly.yuniarti@unisba.ac.id
1
Mustofa Mustofa
mustofafk@ugm.ac.id
2
Teguh Aryandono
teguharyandono@yahoo.com
3
Sofia Mubarika Haryana
sofia.mubarika@gmail.com
4
Department of Biochemistry, Faculty of Medicine Universitas Islam Bandung, Bandung, Indonesia.
Department of Pharmacology and Therapy, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Department of Surgery, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Department of Histology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Objective: Cervical cancer is the second most common cancer among women worldwide, with a high mortality rateespecially in developing countries. Insufficient treatment for cervical cancer, multiple side effects, and high drug pricesencourage researchers to look for effective and selective cancer drugs with appropriate molecular targets. This studyexplored the cytotoxicity of (1,2-epoxy-3(3-(3,4-dimethoxyphenyl)-4H-1-benzopyran-4-on) propane (EPI) synthesizedfrom clove leaves oil on HeLa cells, its combination with doxorubicin (DOX) and cisplatin (CIS), and also their influenceon p53, TIMP-3, and miR-34a as therapeutic targets. Materials and Methods: This research was an experimentalin vitro study on cervical cancer uteri culture. The cytotoxicity was analyzed by MTT assay. The drug combinationsynergisms were indicated by the combination index (CI) (using CompuSyn 1.4). HeLa cells in 32 wells were dividedinto eight groups as negative control, which were given EPI ½IC50, EPI IC50, EPI 2IC50, DOX IC50, combination ofEPI+DOX, CIS, and the combination of EPI+CIS. The p53 and TIMP-3 concentrations were measured using ELISA,and expressions of miR-34a with qRT-PCR. One-way ANOVA and post hoc Tukey tests were performed to determinethe mean difference of all variables between the study groups. Results: IC50 for EPI was 33.24 (±3.01) μg/ml, while DOXand CIS were 4.8 μg/ml (±0.1), and 23.34 μg/ml (±3.01), respectively, while CI values for EPI-DOX were EPI-CIS and 8 (2.93±0.42) μg/ml were significantly higher compared to the control group (p<0.05). All treatment groups showedsignificantly increased miR-34a expressions compared to the control group (p<0.05). Conclusion: The combinationsshowed a very strong synergism and a moderate slight synergism for EPI-DOX and EPI-CIS. Both combinations wereable to increase the expressions of p53, TIMP-3 proteins, and MiR-34a in the HeLa cells.
https://journal.waocp.org/article_69801_6593501283ee67f0d7293006e590b447.pdf
Cervical cancer
Epoxy
MicroRNA-34a
p53
TIMP-3
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2963
2972
10.22034/APJCP.2018.19.10.2963
70495
BRCA1/BRCA2 Mutations Shaped by Ancient Consanguinity Practice in Southern Mediterranean Populations
Fadoua Belaiba
fadoua.belaiba@yahoo.com
1
Imen Medimegh
imen.medimegh@gmail.com
2
Yannick Bidet
yannick.bidet@udamail.fr
3
Sami Boussetta
sami-boussetta@hotmail.com
4
Olfa Baroudi
olfa.baroudi@yahoo.fr
5
Amel Mezlini
amel.mezline@rns.tn
6
Yves Jean Bignon
yves-jean.bignon@cjp.fr
7
Amel Benammar El gaaied
amel.benammarelgaaied@gmail.com
8
Laboratory of Genetics, Immunology and Human Pathology, Faculty of Sciences of Tunis, Université de Tunis El Manar, Campus universitaire1060 El Manar I Tunis, Tunisia.
Laboratory of Genetics, Immunology and Human Pathology, Faculty of Sciences of Tunis, Université de Tunis El Manar, Campus universitaire1060 El Manar I Tunis, Tunisia.
Salah Azaiez Institute of Carcinology of Tunis, Boulevard 09 Avril, 1006 Bab Saadoun, Tunisia.
Laboratory of Genetics, Immunology and Human Pathology, Faculty of Sciences of Tunis, Université de Tunis El Manar, Campus universitaire1060 El Manar I Tunis, Tunisia.
Laboratory of Genetics, Immunology and Human Pathology, Faculty of Sciences of Tunis, Université de Tunis El Manar, Campus universitaire1060 El Manar I Tunis, Tunisia.
Laboratoire Diagnostic Génétique et Moléculaire, Centre Jean-Perrin, 63011 Clermont-Ferrand Cedex 01, France.
Salah Azaiez Institute of Carcinology of Tunis, Boulevard 09 Avril, 1006 Bab Saadoun, Tunisia.
Laboratory of Genetics, Immunology and Human Pathology, Faculty of Sciences of Tunis, Université de Tunis El Manar, Campus universitaire1060 El Manar I Tunis, Tunisia.
The aim of this study is to investigate the involvement of consanguinity on BRCA1/2 mutation incidence in SouthernMediterranean populations and to confirm their low penetrance by comparison of their recurrence in sporadic and familialbreast cancer in a context of ancient consanguinity practice. Our study comprises of two parts: First, a comparison ofthe consanguinity rates of the South Mediterranean countries in a relationship with the frequency of BRCA1 deleteriousmutations in breast cancer families and the recurrence of these mutations. Second, we investigated 23patients with afamily history of breast cancer, 51 patients without a family history of breast cancer using next-generation sequencingof BRCA2 and then confirmed by Sanger sequencing for the novel mutation. As results, we clearly show a strongrelationship between the frequency of BRCA1 deleterious mutations in breast cancer families and rate of consanguinity,since they are significantly inversely correlated. Four deleterious mutations were found in BRCA2 gene including anovel frame-shift mutationc.9382_9383dup in a patient with familial breast cancer and three other frame-shift mutationsc.6591_6592del, c.1310_1313del and c.7654dup in patients with sporadic breast cancer.These results are discussedin a context of selective pressure of ancient consanguinity practice. In conclusion, the study of BRCA1/2 gene inSouthern Mediterranean countries revealed low penetrance recurrent mutations in sporadic and familial breast cancer.These mutations have been selected in a context of ancient consanguinity practice along with protective genetic andenvironmental factors.
https://journal.waocp.org/article_70495_688e7c0a76ddcb90bf32e6b64233500f.pdf
breast cancer
BRCA2/1Mutation analysis
Consanguinity
Southern Mediterranean populations
Recurrence
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2973
2978
10.22034/APJCP.2018.19.10.2973
69127
A Phase IV Efficacy Study of Formeta Plus Carboplatin as First-Line Treatment of Advanced Non-Squamous, Non-Small Cell Lung Cancer in Iran: An Affordable Price with Clinical Benefit
Adnan Khosravi
adkhosravi@yahoo.com
1
Babak Salimi
babak_salmd@yahoo.com
2
Zahra Esfahani-Monfared
yeganenoruzi@yahoo.com
3
Sharareh Seifi
sh_seifi@sbmu.ac.ir
4
Hanifeh Mirtavoos-Mahyari
hanifehmirmah@yahoo.com
5
Tobacco Prevention and Control Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Background: This study performed to assess the efficacy and safety of Formeta (generic form of Pemetrexed) plusCarboplatin as first-line chemotherapy in advanced stage, non- squamous, non small cell lung cancer ( NSCLC) in Iran.Methods: This was a post marketing single-arm phase IV efficacy study of Formeta (manufactured by Oncomed.,CzechRepublic ) and Carboplatin in chemo-naive advanced non-squamous NSCLC Iranian patients. Patients received up to sixcycles of Formeta (500 mg/m2) combined with Carboplatin (area under the curve: AUC 5) every 3 weeks. The primaryendpoint was the progression free survival (PFS) and secondary endpoints were safety and overall survival (OS).Results: Fifty-two patients were enrolled between June 2014 to January 2016, and 44 patients were evaluable for bothsafety and efficacy. Partial and complete responses were achieved in 19 (36.5 %) and 2 (3.8%) patients, respectivelyas well as stable disease in 8 patients (15.3 %). Median of PFS and OS were 7.9 ± 1.1 months and 12.43±0.6 months,respectively. Anemia was the most prevalent adverse events of this regimen. Grades 3 or 4 of adverse events were notobserved in any patients. Non-hematologic and other grades of hematologic toxicities were generally mild, and therewere no treatment-related deaths. Conclusion: The combination of Formeta and Carboplatin was effective in advancednon-squamous NSCLC and can be a suitable candidate as first-line treatment in these patient’s population.
https://journal.waocp.org/article_69127_33070ef0de591ee0c8220c9c4a2f77af.pdf
Non Small Cell Lung Cancer
Carboplatin
Pemetrexed
Chemotherapy
safety
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2979
2984
10.22034/APJCP.2018.19.10.2979
69128
The Effect of Gluten Free Diet on Components of Metabolic Syndrome: A Randomized Clinical Trial
Mehdi Ehteshami
ehteshami.nut@gmail.com
1
Rahebeh Shakerhosseini
rshakerhosseini@yahoo.com
2
Fatemeh Sedaghat
sedaqat_fateme@yahoo.com
3
Mehdi Hedayati
hedayati@endocrine.ac.ir
4
Hassan Eini-Zinab
5
Azita Hekmatdoost
a_hekmat2000@yahoo.com
6
Department of Clinical Nutrition and Dietetics,, Faculty of Nutrition Sciences and Food Technology, National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Clinical Nutrition and Dietetics,, Faculty of Nutrition Sciences and Food Technology, National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Basic Medical Sciences, Faculty of Nutrition Sciences and Food Technology, National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Cellular and Molecular Endocrine Research Center, Research Institute of Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Community Nutrition, Faculty of Nutrition Sciences and Food Technology, National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Clinical Nutrition and Dietetics,, Faculty of Nutrition Sciences and Food Technology, National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Background: This study aimed to assess the effects of Gluten free diet (GFD) on components of metabolic syndrome(MES). Materials and Methods: In this randomized clinical trial, 50 subjects diagnosed with MES were randomlydivided into two groups (n=25). The first group received a GFD and the second group continued their regular diet.Biochemical markers of MES and blood pressure were measured before and after 8-week intervention. Results: Fortyfive subjects completed the study. A post-hoc comparison of the groups showed no effects of the GFD and controldiet on LDL cholesterol, total cholesterol, fasting insulin, HOMA-IR, systolic and diastolic blood pressure levels. TheGFD reduced fasting blood glucose, waist circumference (WC) and serum triglyceride concentration significantlycompared with the control diet (p<0.05). Conclusion: Short-term GFD reduced WC and improved glycemic controland Triglyceride level in subjects with the metabolic syndrome.
https://journal.waocp.org/article_69128_412d1414621884a6053c06f3d5550340.pdf
Gluten free diet
Metabolic Syndrome
Obesity
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2985
2989
10.22034/APJCP.2018.19.10.2985
69806
Anxiety and Depression in Thai Women with Abnormal Cervical Cytology who Attended Colposcopic Unit in Thammasat University Hospital
Sasiwimon Phuakpoolpol
pansasiwimon@gmail.com
1
Komsun Suwannarurk
k_suwannarurk@yahoo.com
2
Kankamol Jaisin
kjaisin@gmail.com
3
Awassada Punyashthira
awassada.pu@gmail.com
4
Junya Pattaraarchachai
dr.junya@gmail.com
5
Kornkarn Bhamarapravatana
drkornkarn@gmail.com
6
Department of Obstetrics and Gynecology, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand.
Department of Obstetrics and Gynecology, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand.
Department of Psychiatry, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand.
Department of Obstetrics and Gynecology, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand.
Chulabhorn International College of Medicine, Thammasat University, Pathum Thani, Thailand.
Department of preclinical Sciences, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand.
Background: Colposcopy is the standard investigation for women with abnormal cervical cytology. Waiting timeto colposcopy could impact to psychological distress. Data about anxiety and depression in eastern countries patientswere limited. Aim of this investigation was to evaluate anxiety and depression status in Thai women who were waitingfor colposcopy compared to women who attended gynecology clinic for cervical cancer screening. Materials andmethods: This study was conducted at the outpatient gynecology clinic of Thammasat University Hospital betweenJanuary 2017 to March 2018. A Total of 200 women were recruited into the study. The cases were divided into the studyand control group. Study group consisted of one hundred women with abnormal cervical cytology referred to colposcopy.One hundred of women who attended gynecology clinic for cervical cancer screening was classified as control group.The Hospital Anxiety and Depression Scale (HADS) was used to determine anxiety in the participants. Results: Totalof 200 women who met criteria of the study were recruited. The mean ages of both groups were 40 years old. Bothgroups were significantly different when it came to their education, 30 and 59 percent of study and control group hadeducation level more than bachelor (p=0.003). The prevalence of anxiety was statistically significantly higher in studygroup than the control group (15% and 6%, respectively). There was only one case of depression in study. Waiting timeand abnormal cervical cytology severity did not affected to anxiety level. Conclusions: Colposcopy and appointmentcaused anxiety. However anxiety was not associated with waiting time to colposcopy or Pap result’s severity.
https://journal.waocp.org/article_69806_32ad0e23010db313c68e938a31da6271.pdf
Abnormal cervical cytology
colposcopy
Anxiety
eng
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
2018-10-01
19
10
2991
2999
10.22034/APJCP.2018.19.10.2991
76497
Metformin Reshapes the Methylation Profile in Breast and Colorectal Cancer Cells
Hussein Sabit
hhsabit@iau.edu.sa
1
Shaimaa E Abdel-Ghany
shaimaa.ibraheem@must.edu.eg
2
Osama A M Said
osama.said@must.edu.eg
3
Mohamed A Mostafa
mohamed.mostafa@must.edu.eg
4
Mokhtar El-Zawahry
mokhtar.elzawahry@must.edu.eg
5
Department of Genetics, Institute for Research and Medical Consultations, Imam Abdulrahman Bin Faisal University, P. O. Box 1982, Dammam, 31441 Saudi Arabia.
College of Biotechnology, Misr University for Science and Technology, Giza, Egypt.
College of Biotechnology, Misr University for Science and Technology, Giza, Egypt.
Center of Basic Science, Misr University for Science and Technology, Giza, Egypt.
College of Biotechnology, Misr University for Science and Technology, Giza, Egypt.
With no sharp cure, breast cancer still be the major and the most serious life-threatening disease worldwide. Colorectalis the third most commonly occurring cancer in men and the second most commonly occurring cancer in women. In thepresent investigation, colon cancer cells (CaCo-2) and breast cancer cells (MCF-7) were treated with elevated dosesof metformin (MET) for 48h. Cell count was assessed using trypan blue test, and the cytotoxicity was evaluated usingMTT assay. Methylation-specific PCR was performed on the bisulfite-treated DNA against two tumor suppressor genes;RASSF1A and RB. Results indicated that: in breast cancer, the cell count was decreased significantly (P>0.005) afterbeing treated with 5, 10, 20, 50, and 100 mM of MET. The elevated concentration had increased reduction percentageson the MCF-7 cells, as 5 mM and 100 mM have yielded 35% and 93.3% reduction in cell viability, respectively. Coloncancer cells have responded to the doses of MET differently, as for the 5 mM and the 100 mM, it gave 88% and 60%reduction in cells viability, respectively. Cytotoxicity assay revealed that 5 mM and 100 mM of MET caused breastcancer cells to loss 61.53% and 85.16% of its viability, respectively, whereas colon cancer cells have responded to the5 mM and 100 mM of MET by reducing the cells viability with 96.91% and 96.24%, respectively. No RB promotermethylation was detected in colon cells, while RASSF1A was partially methylated. In the MCF-7 breast cancer cells,both RASSF1A and RB were partially methylated.
https://journal.waocp.org/article_76497_7a3c179ce8e00317a0d761a74546d3ff.pdf
Metformin
Breast
Colon
RASSF1A
RB