%0 Journal Article %T Screening of N-ras Gene Mutations in Urothelial Cell Carcinomas of the Urinary Bladder in the Kashmiri Population %J Asian Pacific Journal of Cancer Prevention %I West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. %Z 1513-7368 %D 2009 %\ 06/01/2009 %V 10 %N 6 %P 1063-1066 %! Screening of N-ras Gene Mutations in Urothelial Cell Carcinomas of the Urinary Bladder in the Kashmiri Population %K Urothelial cell cancer %K N-ras %K missense mutations %K transversions %R %X Background and Aims: The objective of this study was to assess the frequency of specific-point mutations inN-ras of the RAS gene family in a group of Kashmiri patients with bladder cancer and to observe any associationwith clinicopathological parameters. Methods: Paired tumor and normal tissue specimens of 55 consecutivepatients with urothelial cell carcinoma were screened and DNA was extracted for detection of N-ras activatingmutations in exons 1 and 2. In addition, blood was also collected from all the cases to rule out any germ linemutation. Results: Specific point mutations of activated N-ras were detected in 9% (5 of 55) of the bladdercancer patients, all being missense. The base substitutions identified included three transversions (two G toTand one A to T) and two transitions ( A-G). Sixty % of the mutations were detected in codon 61 and 40% incodon 12. No significant correlations were found between the mutations and clinical features. Conclusion:Although N-ras gene mutation might be one of the mechanisms underlying oncogenesis of urothelial cancer, itseems to be a relatively rare event in Kasmiris, pointing to involvement of different etiological factors in theinduction of bladder tumor in this population %U https://journal.waocp.org/article_25059_20a245a5100a3192eb2b720960bd95ea.pdf