%0 Journal Article %T Enhanced Calreticulin Expression Triggers Apoptosis in the MCF-7 Cell Line %J Asian Pacific Journal of Cancer Prevention %I West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. %Z 1513-7368 %D 2010 %\ 04/01/2010 %V 11 %N 4 %P 1133-1136 %! Enhanced Calreticulin Expression Triggers Apoptosis in the MCF-7 Cell Line %K Calreticulin %K cancer %K Apoptosis %K MTT assay %R %X Calreticulin, since its discovery as a ubiquitous protein of endoplasmic reticulum, has become recognized as a multifunctional luminal resident protein affecting many cellular functions ranging from development to death of a cell. However its role in cancer and significance for therapy remains to be clarified. Here to examine its effects, the calreticulin gene was over expressed in MCF-7 human breast cancer cell lines and influence on apoptosis was examined through an MTT assay. Upon culture in a suitable medium, cells transfected with the calreticulin gene were significantly more susceptible to apoptosis compared to controls. These findings therefore render a novel mechanism by which cancerous cells can be triggered to undergo death by upregulation of a protein resident in the lumen of the endoplasmic reticulum. %U https://journal.waocp.org/article_25338_387128d99d4d492a3ec92029db8a9b91.pdf