%0 Journal Article %T Jaceosidin Induces P53-Dependent G2/M Phase Arrest in U87 Glioblastoma Cells %J Asian Pacific Journal of Cancer Prevention %I West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. %Z 1513-7368 %D 2011 %\ 12/01/2011 %V 12 %N 12 %P 3235-3238 %! Jaceosidin Induces P53-Dependent G2/M Phase Arrest in U87 Glioblastoma Cells %K Jaceosidin %K U87 cells %K G2/M arrest %K p53 %K cyclin B1-CDK1 %R %X Flavonoid compounds have been shown to trigger cell cycle arrest at G0/G1, S and G2/M checkpoints, allowing cells to repair DNA damage before entry into mitosis. Jaceosidin, a flavonoid compound, has been reported to induce apoptosis in various cancer cell lines. In our previous study, we established that jaceosidin induces apoptosis in U87 glioblastoma cells through G2/M phase arrest. However the molecular mechanisms oremained unclear. In the present study, mRNA and protein expression levels of major cell cycle regulatory genes were analyzed by semi-quantitative RT-PCR and Western blot studies respectively. The results demonstrated that jaceosidin-induced G2/M phase arrest in U87 cells is associated with DNA fragmentation, up-regulation of p53 and p21 and subsequent down-regulation of cyclin B1 and CDK1 expression at mRNA as well as at protein level. These findings provide insights into jaceosidin-induced G2/M phase arrest in U87 glioblastoma cells. %U https://journal.waocp.org/article_26044_d8b07494f16455f9a44109015c16b13c.pdf