%0 Journal Article %T Triptolide Inhibits Proliferation and Induces Apoptosis of Human Melanoma A375 Cells %J Asian Pacific Journal of Cancer Prevention %I West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. %Z 1513-7368 %D 2012 %\ 04/01/2012 %V 13 %N 4 %P 1611-1615 %! Triptolide Inhibits Proliferation and Induces Apoptosis of Human Melanoma A375 Cells %K Triptolide %K melanoma A375 cells %K Apoptosis %K caspase %K NF-KB %R %X Triptolide, a diterpenoid obtained from Tripteryglum wilfordii Hook.f, has attracted interest for its antitumoractivities against human tumor cell lines in recent years. This report focuses on anti-proliferative andpro-apoptotic activities in human melanoma A375 cells assessed by CCK8 assay, Hoechst 33258 staining andflow cytometry. In addition, triptolide-induced arrest in the S phase was also observed. Caspase assays showedthe apoptosis induced by triptolide was caspase-dependent and probably through intrinsic apoptotic pathways.Furthermore, expression of NF-kB (p65) and its downstream factors such as Bcl-2, Bcl-XL was down-regulated.Taken together, the data indicate that triptolide inhibits A375 cells proliferation and induces apoptosis by acaspase-dependent pathway and through a NF-kB-mediated mechanism. %U https://journal.waocp.org/article_26381_e0e7068f4dd8356d9dd57751c8b2d966.pdf