%0 Journal Article %T siRNA-mediated Silencing of Notch-1 Enhances Docetaxel Induced Mitotic Arrest and Apoptosis in Prostate Cancer Cells %J Asian Pacific Journal of Cancer Prevention %I West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. %Z 1513-7368 %D 2012 %\ 06/01/2012 %V 13 %N 6 %P 2485-2489 %! siRNA-mediated Silencing of Notch-1 Enhances Docetaxel Induced Mitotic Arrest and Apoptosis in Prostate Cancer Cells %K Notch signaling - prostate cancer - siRNA - cell cycle %K apoptosis - p21waf1/cip1 - Akt %R %X Purpose: Notch is an important signaling pathway that regulates cell fate, stem cell maintenance and theinitiation of differentiation in many tissues. It has been reported that activation of Notch-1 contributes totumorigenesis. However, whether Notch signaling might have a role in chemoresistance of prostate cancer isunclear. This study aimed to investigate the effects of Notch-1 silencing on the sensitivity of prostate cancercells to docetaxel treatment. Methods: siRNA against Notch-1 was transfected into PC-3 prostate cancer cells.Proliferation, apoptosis and cell cycle distribution were examined in the presence or absence of docetaxel byMTT and flow cytometry. Expression of p21waf1/cip1 and Akt as well as activation of Akt in PC-3 cells were detectedby Western blot and Real-time PCR. Results: Silencing of Notch-1 promoted docetaxel induced cell growthinhibition, apoptosis and cell cycle arrest in PC-3 cells. In addition, these effects were associated with increasedp21waf1/cip1 expression and decreased Akt expression and activation in PC-3 cells. Conclusion: Notch-1 promoteschemoresistance of prostate cancer and could be a potential therapeutic target. %U https://journal.waocp.org/article_26528_f408cbef6c7b424dac02477211caf29a.pdf