%0 Journal Article %T Anti-tumor Efficacy of a Hepatocellular Carcinoma Vaccine Based on Dendritic Cells Combined with Tumor-derived Autophagosomes in Murine Models %J Asian Pacific Journal of Cancer Prevention %I West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. %Z 1513-7368 %D 2013 %\ 05/01/2013 %V 14 %N 5 %P 3109-3116 %! Anti-tumor Efficacy of a Hepatocellular Carcinoma Vaccine Based on Dendritic Cells Combined with Tumor-derived Autophagosomes in Murine Models %K Hepatocellular carcinoma %K Dendritic Cells %K Autophagy %K Immunotherapy %K tumor antigen %K cross-presentation %R %X The majority of hepatocellular carcinoma (HCC) patients have a poor prognosis with current therapies, andnew approaches are urgently needed. We have developed a novel therapeutic cancer vaccine platform based ontumor cell derived autophagosomes (DRibbles) for cancer immunotherapy. We here evaluated the effectivenessof DRibbles-pulsed dendritic cell (DC) immunization to induce anti-tumor immunity in BALB/c mouse HCCand humanized HCC mouse models generated by transplantation of human HCC cells (HepG2) into BALB/c-numice. DRibbles were enriched from H22 or BNL cells, BALB/c-derived HCC cell lines, by inducing autophagyand blocking protein degradation. DRibbles-pulsed DC immunization induced a specific T cell response againstHCC and resulted in significant inhibition of tumor growth compared to mice treated with DCs alone. Antitumorefficacy of the DCs-DRibbles vaccine was also demonstrated in a humanized HCC mouse model. Theresults indicated that HCC/DRibbles-pulsed DCs immunotherapy might be useful for suppressing the growthof residual tumors after primary therapy of human HCC. %U https://journal.waocp.org/article_27752_167d61d256f31f76573ee568384b3907.pdf