%0 Journal Article %T Experimental Study of Endostar Injection Concomitant with Cryoablation on Lung Adenocarcinoma A549 Xenografts %J Asian Pacific Journal of Cancer Prevention %I West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. %Z 1513-7368 %D 2013 %\ 11/01/2013 %V 14 %N 11 %P 6697-6701 %! Experimental Study of Endostar Injection Concomitant with Cryoablation on Lung Adenocarcinoma A549 Xenografts %K Endostar %K cryoablation %K lung adenocarcinoma A549 xenografts %K VEGF %K micro-vessel density %R %X Objective: To explore the inhibiting effect and mechanism of Endostar injection concomitant with cryoablationon lung adenocarcinoma A549 xenografts in nude mice. Materials and Methods: A total of 24 nude mice withsubcutaneous xenografts of the A549 cell line were established and divided into 4 groups when the maximaldiameters of tumors became 1 cm: control group, Endostar group, cryoablation group and combination group(Endostar concomitant with cryoablation). The nude mice were sacrificed after 21-days treatment, tumourtissues were removed to measure their volume, in situ test of TdT-mediated dUTP nick end labeling (TUNEL)was adopted to determine the cellular apoptosis around freezing injury zones, and immunohistochemical SPtest was applied for the detection of micro-vessel density (MVD) and vascular endothelial growth factor (VEGF)expression levels. Results: At 21-days after treatment, the growth velocities of control group, Endostar group,cryoablation group and combination group were 236.7±51.2%, 220.0±30.6%, 159.5±29.3% and 103.3±25.5%(P<0.01), while cellular apoptosis rates of tumors were 21.7±2.34%, (22.17±1.47)%, 38.3±1.37% and 49.2±1.72%,(P<0.01), respectively, according to the immunohistochemical test. MVD and VEGF expression levels in thecombination group were both lower than in other groups (P<0.01), also being positively related (r=0.925, P<0.01).Conclusions: Endostar can significantly improve the inhibitory effects of cryoablation on xenografts of lungadenocarcinoma A549, and the mechanism is probably associated with its function as an inhibitor of tumourneo-angiogenesis through down-regulating VEGF expression. %U https://journal.waocp.org/article_28357_b73d5aa2390c1644e5b58640f2cd6c3b.pdf