%0 Journal Article %T Differential microRNA Expression by Solexa Sequencing in the Sera of Ovarian Cancer Patients %J Asian Pacific Journal of Cancer Prevention %I West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. %Z 1513-7368 %D 2014 %\ 04/01/2014 %V 15 %N 4 %P 1739-1743 %! Differential microRNA Expression by Solexa Sequencing in the Sera of Ovarian Cancer Patients %K Ovarian Cancer %K Serum %K MicroRNAs %K Solexa sequencing %K diagnostic biomarkers %R %X MicroRNAs are a class of small noncoding RNA which play important regulatory roles in a variety of cancers.MiRNA-specific expression profiles have been reported for several pathological conditions. In this study, wecombined large scale parallel Solexa sequencing to identify 11 up-regulated miRNAs and 19 down-regulatedmiRNAs with computational techniques in the sera of ovarian cancer patients while using healthy serum as thecontrol. Among the above, four miRNAs (miR-22, miR-93, miR-106b, miR-451) were validated by quantitativeRT-PCR and found to be significantly aberrantly expressed in the serum of ovarian cancer patients (P<0.05).There were no significant differences between samples from cancer stage Ⅰ/Ⅱ and Ⅲ/Ⅳ. However, the levelsof miR-106b (p=0.003) and miR-451 (p=0.007) were significantly different in those patients under and over 51yearsof age. MiR-451 and miR-93 were also specific when analyzed with reference to different levels of CA125.This study shows that Solexa sequencing provides a promising method for cancer-related miRNA profiling, andselectively expressed miRNAs could be used as potential serum-based biomarkers for ovarian cancer diagnosis. %U https://journal.waocp.org/article_28822_8d95fcdbddf2ea04efdf3d1f644eb94c.pdf