%0 Journal Article %T Extract of Saccharina japonica Induces Apoptosis companied by Cell Cycle Arrest and Endoplasmic Reticulum Stress in SK-Hep1 Human Hepatocellular Carcinoma Cells %J Asian Pacific Journal of Cancer Prevention %I West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. %Z 1513-7368 %D 2014 %\ 07/01/2014 %V 15 %N 7 %P 2993-2999 %! Extract of Saccharina japonica Induces Apoptosis companied by Cell Cycle Arrest and Endoplasmic Reticulum Stress in SK-Hep1 Human Hepatocellular Carcinoma Cells %K Caspase-dependent/independent apoptosis %K Hep1 cells %K cell cycle arrest %K ER-stress %K Saccharina japonica %R %X Saccharina japonica is a family member of Phaeophyceae (brown macro-alga) and extensively cultivatedin China, Japan and Korea. Here, the potential anti-cancer effect of n-hexane fraction of S. japonica wasevaluated in SK-Hep1 human hepatocellular carcinoma cells. The N-hexane fraction reduced cell viability andincreased the numbers of apoptotic cells in a both dose- and time-dependent manner. Apoptosis was activatedby both caspase-dependent and independent pathways. The caspase-dependent cell death pathway is mediatedby cell surface death receptors and activated caspase-8 amplified the apoptotic signal either through directactivation of downstream caspase-3 or pro-apoptotic proteins (Bad, Bax and Bak) subsequently leading to therelease of cytochrome c. On the other hand, caspase-independent apoptosis appeared mediated by disruptionof mitochondrial membrane potential and translocation of AIF to the nucleus where they induced chromatincondensation and/or large-scale DNA fragmentation. In addition, the n-hexane fraction induced endoplasmicreticulum (ER)-stress and cell cycle arrest. The results suggested that potential anti-cancer effects of n-hexaneextract from S. japonica on SK-Hep1 cells. %U https://journal.waocp.org/article_29032_a17512254e843383b614e07f9c8198c8.pdf