%0 Journal Article %T CCNA1 Promoter Methylation: a Potential Marker for Grading Papanicolaou Smear Cervical Squamous Intraepithelial Lesions %J Asian Pacific Journal of Cancer Prevention %I West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. %Z 1513-7368 %D 2014 %\ 12/01/2014 %V 15 %N 18 %P 7971-7975 %! CCNA1 Promoter Methylation: a Potential Marker for Grading Papanicolaou Smear Cervical Squamous Intraepithelial Lesions %K CCNA1 promoter methylation %K cervical cancer %K HPV %K duplex MS-PCR %K Pap smear %R %X Background: From our previous study, we established that cyclin A1 (CCNA1) promoter methylation isstrongly correlated with multistep progression of HPV-associated cervical cancer, suggesting potential use as adiagnostic maker of disease. Objectives: The purpose of the present study was to assess the prevalence of CCNA1promoter methylation in residual cervical cells isolated from liquid-based cytology that underwent hrHPV DNAscreening for cervical cancer, and then to evaluate this marker for diagnostic accuracy using parameters likesensitivity, specificity, predictive values and likelihood ratio. Methods: In this retrospective study, histopathologywas used as the gold standard method with specimens separated into the following groups: negative (n=31), lowgradesquamous intraepithelial lesions (LSIL, n=34) and high-grade squamous intraepithelial lesions or worse(HSIL+, n=32). The hrHPV was detected by Hybrid Capture 2 (HC2) and CCNA1 promoter methylation wasexamined by CCNA1 duplex methylation specific PCR. Results: The results showed the frequencies of CCNA1promoter methylation were 0%, 5.88% and 83.33%, while the percentages of hrHPV were 66.67%, 82.35% and100% in the negative, LSIL and HSIL+ groups, respectively. Although hrHPV infection showed high frequencyin all three groups, it could not differentiate between the different groups and grades of precancerous lesions. Incontrast, CCNA1 promoter methylation clearly distinguished between negative/LSIL and HSIL+, with high levelsof all statistic parameters. Conclusion: CCNA1 promoter methylation is a potential marker for distinguishingbetween histologic negative/LSIL and HSIL+using cervical cytology samples. %U https://journal.waocp.org/article_29868_b69d7a13735058ad499082f49f8bab0a.pdf