%0 Journal Article %T miR-205 in Situ Expression and Localization in Head and Neck Tumors - a Tissue Array Study %J Asian Pacific Journal of Cancer Prevention %I West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. %Z 1513-7368 %D 2014 %\ 12/01/2014 %V 15 %N 21 %P 9071-9075 %! miR-205 in Situ Expression and Localization in Head and Neck Tumors - a Tissue Array Study %K MiR-205 %K Head and neck cancers %K FFPE %K locked nucleic acid %K in situ hybridization %K tumor tissue array %R %X Background: microRNAs are small non-coding RNA that control gene expression by mRNA degradation ortranslational inhibition. These molecules are known to play essential roles in many biological and physiologicalprocesses. miR-205 may be differentially expressed in head and neck cancers; however, there are conflictingdata and localization of expression has yet to be determined. Materials and Methods: miR-205 expression wasinvestigated in 48 cases of inflammatory, benign and malignant tumor tissue array of the neck, oronasopharynx,larynx and salivary glands by Locked Nucleic Acid in situ hybridization (LNA-ISH) technology. Results: miR-205expression was significantly differentially expressed across all of the inflammatory, benign and malignant tumortissues of the neck. A significant increase in miR-205 staining intensity (p<0.05) was observed from inflammationto benign and malignant tumors in head and neck tissue array, suggesting that miR-205 could be a biomarkerto differentiate between cancer and non-cancer tissues. Conclusions: LNA-ISH revealed that miR-205 exhibitedsignificant differential cytoplasmic and nuclear staining among inflammation, benign and malignant tumors ofhead and neck. miR-205 was not only exclusively expressed in squamous epithelial malignancy. This study offersinformation and a basis for a comprehensive study of the role of miR-205 that may be useful as a biomarkerand/or therapeutic target in head and neck tumors. %U https://journal.waocp.org/article_30052_daa7f9c95c6cd84e700b07d5841ee3dd.pdf