%0 Journal Article %T Prognostic Significance of Hes-1, a Downstream Target of Notch Signaling in Hepatocellular Carcinoma %J Asian Pacific Journal of Cancer Prevention %I West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. %Z 1513-7368 %D 2015 %\ 09/01/2015 %V 16 %N 9 %P 3811-3816 %! Prognostic Significance of Hes-1, a Downstream Target of Notch Signaling in Hepatocellular Carcinoma %K Hepatocellular carcinoma %K lung metastasis %K notch-1 %K Hes-1 %K Shh %R %X Background: Hairy and enhancer of split 1 (Hes-1) protein is a downstream target of Notch signaling andis a basic helix-loop-helix transcriptional repressor. However, definitive evidence for a role in hepatocellularcarcinoma (HCC) cells has not been reported. Here, Hes-1 was revealed to an important component of the Notchsignaling cascade in HCC cell lines possessing different potential for lung metastasis. Materials and Methods:RNAi mediated by plasmid constructs was used to analyze the role of Hes-1 in MHCC-97L HCC cells by assessingproliferation, apoptosis, cell migration and matrigel invasion following transfection. Hes-1 protein expressionanalysis in HCC tissue was also conducted by immunohistochemistry. Results: Our studies revealed that Hes-1was decreased in HCC cell lines with higher lung metastasis potential at both the mRNA and protein levels.Down-regulation of the Hes-1 gene in MHCC-97L cells resulted in increased cell proliferation, reduced apoptosisand increased migration and invasion. Conclusions: Hes-1 has potential prognostic value in post-surgical HCCpatients and may be an independent prognostic indicator for overall survival and tumor recurrence. Thesefindings have important implications for understanding the mechanisms by which Hes-1 participates in tumorproliferation and invasion. %U https://journal.waocp.org/article_30993_136ecd3853ae1236d60fceb1a546b295.pdf