%0 Journal Article %T Comparison Between Two Detection Methods for HPV16,HPV18 and P16Ink4a Biomarkers in Diagnosis of Abnormal Cervical Cytology %J Asian Pacific Journal of Cancer Prevention %I West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. %Z 1513-7368 %A Khazaei, Sdigheh %A Izadi, Babak %A Mirbahari, Seyed Ghasem %A Madani, Seyed Hamid %A Malek Khosravi, Shohre %A Emami Alagha, Mohsen %A Sajadimajd, Soraya %D 2016 %\ 12/01/2016 %V 17 %N 12 %P 5223-5227 %! Comparison Between Two Detection Methods for HPV16,HPV18 and P16Ink4a Biomarkers in Diagnosis of Abnormal Cervical Cytology %K dysplasia %K Cervix %K HPV 16 %K HPV 18 %R 10.22034/APJCP.2016.17.12.5223 %X   Background: Cervical cancer is the second most common cancer among women in many populations. While the Pap smear is a well established screening test it suffers from both false-positive and false-negative results in diagnosis of cancers and precancerous states. In this study, immunocytochemistry of the P16 biomarker and HPV-PCR were compared for their diagnostic potential. Materials and methods: In the study, we obtained pairs of specimens from 45 women with cervical dysplasia. One sample was placed in a liquid-based solution, and processed for staining of sections with antibodies to P16. HPV-PCR was performed on the other and the results obtained were analyzed by T-test using SPSS v. 15. Results: Using HPV-PCR 71% of the samples were found to be infected with either HPV 16 or HPV 18, and the rate of infection did not have a statistically significant relationship with higher grades of dysplasia (p= 0.253). In contrast, with immunocytochemistry evaluation of P16, 64% of the specimens were positive, but the percentage of positive results significantly increased with higher grades of dysplasia (p= 0.0001). Conclusion: Employment of the P16 marker as an optional test might be preferable over HPV-PCR for cervical dysplasia in our geographical region. %U https://journal.waocp.org/article_42105_8bc06b737b09d67b04bf3b62e0b13c83.pdf