%0 Journal Article %T Novel Mutations of the CHRNA3 Gene in Non-Small Cell Lung Cancer in an Iranian Population %J Asian Pacific Journal of Cancer Prevention %I West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. %Z 1513-7368 %A Mehrabi, Nahid %A Moshtaghioun, Seyed Mohammad %A Neamatzadeh, Hossein %D 2017 %\ 01/01/2017 %V 18 %N 1 %P 253-255 %! Novel Mutations of the CHRNA3 Gene in Non-Small Cell Lung Cancer in an Iranian Population %K non-small cell lung cancer %K CHRNA3 %K SSCPs %K Polymorphism %K rs8040868 %R 10.22034/APJCP.2017.18.1.253 %X   Background: Lung cancer, the leading cause of cancer-related worldwide deaths, largely results from the combined effects of smoking exposure and genetic susceptibility. CHRNA3, a nicotinic acetylcholine receptor gene, is associated with lung cancer risk. This study sought to identify variations in exon 3 of CHRNA3 in an Iranian population with non-small cell lung cancer (NSCLC). Materials and methods: A case-control study including 147 individuals with lung cancer and 145 healthy individuals was conducted. As mobility shift caused by nucleotide substitutions might be due to a conformational change of single-stranded DNAs, we designated these as single-strand conformation polymorphisms (SSCPs). PCR amplified products with SSCP were subjected to DNA sequencing. Results: The sequencing results showed 3 polymorphisms in exon 3 of CHRNA3, rs8040868, rs763384023 and rs2869547 , the latter two of which have not been reported in NSCLC, previously. Conclusion: It appears that the rs8040868 may be considered as a pathogenic mutation associated with the clinical phenotype. Polymorphisms are important factors for development of cancers and may provide additional insights into mechanisms underlying NSCLC. %U https://journal.waocp.org/article_42937_e465638db6a883ac3f47a3f4aa19088f.pdf