%0 Journal Article %T Cytotoxic Activity of Propolis Extracts from the Stingless Bee Trigona Sirindhornae Against Primary and Metastatic Head and Neck Cancer Cell Lines %J Asian Pacific Journal of Cancer Prevention %I West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. %Z 1513-7368 %A Utispan, Kusumawadee %A Chitkul, Bordin %A Koontongkaew, Sittichai %D 2017 %\ 04/01/2017 %V 18 %N 4 %P 1051-1055 %! Cytotoxic Activity of Propolis Extracts from the Stingless Bee Trigona Sirindhornae Against Primary and Metastatic Head and Neck Cancer Cell Lines %K Cytotoxic activity %K HNSCC cell lines %K Trigona sirindhornae propolis %R 10.22034/APJCP.2017.18.4.1051 %X Background: Propolis, a resinous substance produced by the honeybee, has a wide spectrum of potent biologicalactivities. However, anti-cancer activity of propolis obtained from Trigona sirindhornae, a new species of stinglessbee, has not yet been reported. This study concerned cytotoxicity of propolis extracts from T. sirindhornae against twohead and neck squamous cell carcinoma (HNSCC) cell lines. Materials and Methods: A dichloromethane extractof propolis (DMEP) was prepared generating 3 fractions: DMEP-A, DMEP-B, and DMEP-C. Genetically-matchedHNSCC cell lines derived from primary (HN30) and metastatic sites (HN31) in the same patient were used to studycytotoxic effects of the DMEPs by MTT assays. The active compounds in the DMEPs were analyzed by reversephasehigh performance liquid chromatography. Results: DMEP-A exhibited cytotoxic activity on HN30 cells withsignificantly decreased viability at 200 μg/ml compared with the control (p<0.05). However, no significant cytotoxiceffect was evident in HN31 cells. DMEP-B and DMEP-C significantly decreased the viability of both cell lines from100–200 μg/ml and 50–200 μg/ml, respectively (p<0.05). Interestingly, HN31 cells were more toxically sensitivecompared with the HN30 cells when treated with DMEP-B and DMEP-C. IC50 values for DMEP-B with HN30 andHN31 cells were more than 200 μg/ml and 199.8±1.05 μg/ml, respectively. The IC50 of DMEP-C to HN30 and HN31cells was found to be 114.3±1.29 and 76.33±1.24 μg/ml, respectively. Notably, apigenin, pinocembrin, p-coumaric acid,and caffeic acid were not detected in our propolis extracts. Conclusion: T. sirindhornae produced propolis displayscytotoxic effects against HNSCC cells s. Moreover, DMEP-B and DMEP-C differentially inhibited the proliferationof a metastatic HNSCC cell line. %U https://journal.waocp.org/article_46095_cbb9c5d50131e5f7851c639f933abea6.pdf