%0 Journal Article %T Evaluation of Osteopontin as a Biomarker in Hepatocellular Carcinomas in Egyptian Patients with Chronic HCV Cirrhosis %J Asian Pacific Journal of Cancer Prevention %I West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. %Z 1513-7368 %A Abdel-Hafiz, Samah Mamdouh %A Hamdy, Hussam EM %A Khorshed, Fatma M %A Aboushousha, Tarek S %A Safwat, Gehan %A Saber, mohamed A %A Seleem, Mohamed %A Soliman, Amira H %D 2018 %\ 04/01/2018 %V 19 %N 4 %P 1021-1027 %! Evaluation of Osteopontin as a Biomarker in Hepatocellular Carcinomas in Egyptian Patients with Chronic HCV Cirrhosis %K Osteopontin %K Alpha-fetoprotein %K Hepatocellular carcinoma %K Real-Time PCR %R 10.22034/APJCP.2018.19.4.1021 %X Background: Hepatocellular carcinoma (HCC) is a high incidence disease in Egypt with a poor prognosis andsurvival. Biomarkers are important for diagnosis of HCC at an early stage. Osteopontin (OPN), a glycoprotein secreted bymacrophages, osteoblasts, and T cells, is also highly expressed in a variety of tumors, such as examples in the breast, colon,and stomach. The present study aimed to correlate the serum level of OPN in HCV-positive hepatocellular carcinomapatients, with OPN expression in tumor and non-tumor liver tissues in order to identify its efficacy as a biomarkerfor diagnosis. Material and Methods: Out of total of 146 patients, 80 were selected for inclusion in the study. Bloodsamples as well as specimens of tumor and non-tumor liver tissue were collected. In addition, blood samples from 20healthy volunteers were obtained as controls. Serum OPN and alpha-fetoprotein (AFP) were evaluated by ELISA forHCC and control groups. OPN and AFP gene expression were examined by real-time PCR, after homogenization andDNA extraction from serum samples and liver tissues. Results: It was found that serum OPN levels were significantlyhigher in the HCC group compared to normal group (P=0.009), with a strong positive correlation with AFP expression.However, there was no significant difference between OPN expression in tumor and non-tumor liver tissue. Conclusion:Serum OPN is highly suggested to be a professional candidate for HCC early diagnosis, with a diagnostic ability andaccuracy equal or higher than for AFP. %U https://journal.waocp.org/article_59746_c023a6d546c7c7a17632fcd6807bec9d.pdf