%0 Journal Article %T Evaluation of Anticancer Activity of Camellia Sinensis in the Caco-2 Colorectal Cancer Cell Line %J Asian Pacific Journal of Cancer Prevention %I West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. %Z 1513-7368 %A Esghaei, Maryam %A Ghaffari, Hadi %A Rahimi Esboei, Bahman %A Ebrahimi Tapeh, Zienab %A Bokharaei-Salim, Farah %A Motevalian, Manijeh %D 2018 %\ 06/01/2018 %V 19 %N 6 %P 1697-1701 %! Evaluation of Anticancer Activity of Camellia Sinensis in the Caco-2 Colorectal Cancer Cell Line %K Tea %K Camellia sinensis %K Caco-2 Cells %K Cisplatin %K Aquaporin 5 %R 10.22034/APJCP.2018.19.6.1697 %X Background: Colorectal cancer (CRC) is widespread across the world. While conventional anticancer treatmentscan help the affected patients, cells of vital organs such as the kidney, lungs, bladder and nervous system maysuffer from side effects of chemotherapeutic drugs, so that it is necessary to search for alternatives. From ancienttimes, attention has focused on medicinal plants and natural products. In the current work, Camellia sinensis, whoseleaves are used to produce green tea was evaluated for anticancer effects in cell culture. Materials and Methods:A hydroalcoholic extract of Camellia sinensis young leaves was prepared by percolation and compared with Cisplatinas a known anticancer drug for effects on two cell lines: Caco-2, colon carcinoma cells, and mouse normal fibroblasts(L929). Cytotoxicity of 50, 100, 200, 400 and 800 μg/ml of Camellia sinensis extract was evaluated by MTT assay andaquaporin 5 (AQP5), detected as a biomarker for surviving cells using immunofluorescence microscopy. Results: MTTassays with hydroalcoholic extract of Camellia sinensis showed considerable inhibition of growth of Caco-2 cells,significant at 800 μg/ml (P<0.05), with little effect on L929 cells. Levels of aquaporin 5 protein decreased in Caco-2cell culture following green tea extract treatment. Conclusion: According to the results of the current study, Camelliasinensis is a medicinal plant with potent anticancer influence which might be specific. %U https://journal.waocp.org/article_63279_c71d684550a1b46e1c8d3f96c8656773.pdf