%0 Journal Article %T Mesenchymal Stromal Stem Cell-Derived Microvesicles Enhance Tumor Lysate Pulsed Dendritic Cell Stimulated Autologous T lymphocyte Cytotoxicity %J Asian Pacific Journal of Cancer Prevention %I West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. %Z 1513-7368 %A Rahmani Kukia, Nasim %A Alipanah-Moghadam, Reza %A Delirezh, Nowruz %A Mazani, Mohammad %D 2018 %\ 07/01/2018 %V 19 %N 7 %P 1895-1902 %! Mesenchymal Stromal Stem Cell-Derived Microvesicles Enhance Tumor Lysate Pulsed Dendritic Cell Stimulated Autologous T lymphocyte Cytotoxicity %K Glial cells %K Tumor cell lysate %K Dendritic Cells %K MSC1-derived MVs %K cancer immunotherapy %R 10.22034/APJCP.2018.19.7.1895 %X Background: Immunotherapy is one promising therapeutic strategy against glioma, an aggressive form of braincancer. Previous studies have demonstrated that multiple tumor antigens exist and can be used to induce tumor specificT cell responses. Furthermore, recently it was shown that TLR4-primed mesenchymal stem cells (MSCs), also knownas MSC1, mostly elaborate pro-inflammatory mediators. Compared to MSCs, MSC-derived microvesicles (MVs) haveadvantageous properties that present them as stable, long lasting effectors with no risk of immune rejection. Therefore,peripheral blood monocyte derived dendritic cells (MoDCs) have been used to load tumor antigens and stimulate Tcell mediated responses in the presence of MSC1-derived MVs in vitro. Methods: The B92 tumor cell line was heatedto 43°C for 90 min prior to preparation of tumor cell lysates. MVs were purified by differential ultracentrifugationafter isolation, stimulation of proliferation and treatment of MSCs. Autologous T cells isolated from non-adherentcells were harvested during the procedure to generate MoDCs and then incubated with heat stressed tumor cell lysatepulsed DCs in the presence of MSC1-derived MVs. T cells were then co-cultured with tumor cells in 96-well plates ata final volume of 200 μl CM at an effector: target ratio of 100:1 to determine their specific cytotoxic activity. Results:Flow cytometric analysis, T cell mediated cytotoxicity showed that heat stressed tumor antigen pulsed MoDCs andMSC1-derived MVs primed T cells elicited non-significantly enhanced cytotoxic activity toward B92 tumor cells(P≥0.05). Conclusion: These findings may offer new insights into tumor antigen presenting technology involvingdendritic cells and MSC1-derived MVs. Further exploration of the potential of such nanoscale particles in immunotherapyand in novel cancer vaccine settings appears warranted. %U https://journal.waocp.org/article_65367_a7231c8a732b0bd27a47bde4c6565ee9.pdf