%0 Journal Article %T Association of Elevated Peripheral Blood Micronucleus Frequency and Bmi-1 mRNA Expression with Metastasis in Iranian Breast Cancer Patients %J Asian Pacific Journal of Cancer Prevention %I West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. %Z 1513-7368 %A Salimi, Mahdieh %A Eskandari, Elaheh %D 2018 %\ 10/01/2018 %V 19 %N 10 %P 2723-2730 %! Association of Elevated Peripheral Blood Micronucleus Frequency and Bmi-1 mRNA Expression with Metastasis in Iranian Breast Cancer Patients %K breast cancer %K Gene expression %K micronucleus %K metastasis biomarkers %K oxidative stress %R 10.22034/APJCP.2018.19.10.2723 %X Background: In order to find cytogenetic and molecular metastasis biomarkers detectable in peripheral blood thespontaneous genomic instability expressed as micronuclei and Bmi-1 expression in peripheral blood of breast cancer(BC) patients were studied in different stages of the disease compared with unaffected first-degree relatives (FDRs)and normal control. Methods: The Cytokinesis Block Micronuclei Cytome (CBMN cyt) and nested real-time ReverseTranscription-Polymerase Chain Reaction (RT-PCR) assays, were respectively used to measure genomic instability andBmi-1 gene expression in 160 Iranian individuals comprised of BC patients in different stages of the disease, unaffectedFDRs and normal control groups. Result: The frequency of micronuclei and Bmi-1 expression were dramatically higherin distant metastasis compared with non-metastatic BC. In spite of micronucleus frequency with no association withlymph node (LN) involvement and hormone receptor status, the Bmi-1 expression level was higher in LN positive andtriple negative patients. Conclusion: Our results indicate that increased genomic instability expressed as micronuclei andhigher Bmi-1 expression in peripheral blood are associated with metastasis in breast cancer. Therefore implementationof micronucleus assay and Bmi-1 expression analysis in blood as possible cytogenetic and molecular biomarkers inclinical level may potentially enhance the quality of management of patients with breast cancer. %U https://journal.waocp.org/article_69112_1ca3749ae3a6b03b9ade2dd125ccfd4a.pdf